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UniProtKB - Q8LB17 (RBL15_ARATH)
Protein
Rhomboid-like protein 15
Gene
RBL15
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Probable rhomboid-type serine protease that catalyzes intramembrane proteolysis. May function in senescence.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 145 | NucleophileBy similarity | 1 | |
Active sitei | 197 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- misfolded protein binding Source: GO_Central
- serine-type endopeptidase activity Source: InterPro
- ubiquitin-specific protease binding Source: GO_Central
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: GO_Central
- ubiquitin-dependent ERAD pathway Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease |
Names & Taxonomyi
Protein namesi | Recommended name: Rhomboid-like protein 151 Publication (EC:3.4.21.-Curated)Short name: AtRBL151 Publication |
Gene namesi | Name:RBL151 Publication Synonyms:RBL111 Publication Ordered Locus Names:At3g58460Imported ORF Names:F14P22.50Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G58460 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- Hrd1p ubiquitin ligase ERAD-L complex Source: GO_Central
- integral component of endoplasmic reticulum membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 70 – 90 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 103 – 123 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 141 – 161 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 176 – 196 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 198 – 218 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220607 | 1 – 403 | Rhomboid-like protein 15Add BLAST | 403 |
Proteomic databases
PaxDbi | Q8LB17 |
ProteomicsDBi | 236512 [Q8LB17-1] |
PTM databases
iPTMneti | Q8LB17 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q8LB17, baseline and differential |
Genevisiblei | Q8LB17, AT |
Interactioni
GO - Molecular functioni
- misfolded protein binding Source: GO_Central
- ubiquitin-specific protease binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 10330, 8 interactors |
IntActi | Q8LB17, 8 interactors |
STRINGi | 3702.AT3G58460.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8LB17 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8LB17 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 361 – 401 | UBAPROSITE-ProRule annotationAdd BLAST | 41 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 282 – 316 | DisorderedSequence analysisAdd BLAST | 35 |
Sequence similaritiesi
Belongs to the peptidase S54 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2632, Eukaryota |
HOGENOMi | CLU_036445_1_0_1 |
InParanoidi | Q8LB17 |
OMAi | QWWENIP |
PhylomeDBi | Q8LB17 |
Family and domain databases
Gene3Di | 1.20.1540.10, 1 hit |
InterProi | View protein in InterPro IPR022764, Peptidase_S54_rhomboid_dom IPR035952, Rhomboid-like_sf IPR015940, UBA IPR009060, UBA-like_sf |
Pfami | View protein in Pfam PF01694, Rhomboid, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00165, UBA, 1 hit |
SUPFAMi | SSF144091, SSF144091, 1 hit SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS50030, UBA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8LB17-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPNIVTEAG VQTRVGQWWN AIPFLTSSVV VVCGVIYLIC LLTGYDTFYE
60 70 80 90 100
VCFLPSAIIS RFQVYRFYTA IIFHGSLLHV LFNMMALVPM GSELERIMGS
110 120 130 140 150
VRLLYLTVLL ATTNAVLHLL IASLAGYNPF YQYDHLMNEC AIGFSGILFS
160 170 180 190 200
MIVIETSLSG VTSRSVFGLF NVPAKLYPWI LLIVFQLLMT NVSLLGHLCG
210 220 230 240 250
ILSGFSYSYG LFNFLMPGSS FFTTIESASW MSSFIRRPKF IMCTGGNPSS
260 270 280 290 300
YIPTYSAQNT TSSGFSTGNA WRSLSSWLPQ REASNQSSED SRFPGRGRTL
310 320 330 340 350
STARDPTAPA GETDPNLHAR LLEDSSSPDR LSDATVNTVA DSRQAPIANA
360 370 380 390 400
AVLPQSQGRV AASEEQIQKL VAMGFDRTQV EVALAAADDD LTVAVEILMS
QQA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4J5V3 | F4J5V3_ARATH | RHOMBOID-like protein 15 | RBL15 ATRBL11, ATRBL15, RBL11, rhomboid-like protein 11, RHOMBOID-like protein 15 | 426 | Annotation score: |
Sequence cautioni
The sequence CAB68184 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 237 | R → Q in AAM65024 (PubMed:14593172).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057725 | 358 – 362 | GRVAA → VTFLL in isoform 2. | 5 | |
Alternative sequenceiVSP_057726 | 363 – 403 | Missing in isoform 2. Add BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL137082 Genomic DNA Translation: CAB68184.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79784.1 AY136386 mRNA Translation: AAM97052.1 BT000186 mRNA Translation: AAN15505.1 AK318905 mRNA Translation: BAH57020.1 AY087480 mRNA Translation: AAM65024.1 |
PIRi | T45666 |
RefSeqi | NP_567064.1, NM_115708.4 [Q8LB17-1] |
Genome annotation databases
EnsemblPlantsi | AT3G58460.1; AT3G58460.1; AT3G58460 [Q8LB17-1] |
GeneIDi | 825015 |
Gramenei | AT3G58460.1; AT3G58460.1; AT3G58460 [Q8LB17-1] |
KEGGi | ath:AT3G58460 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL137082 Genomic DNA Translation: CAB68184.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79784.1 AY136386 mRNA Translation: AAM97052.1 BT000186 mRNA Translation: AAN15505.1 AK318905 mRNA Translation: BAH57020.1 AY087480 mRNA Translation: AAM65024.1 |
PIRi | T45666 |
RefSeqi | NP_567064.1, NM_115708.4 [Q8LB17-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1VG5 | NMR | - | A | 342-401 | [»] | |
SMRi | Q8LB17 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 10330, 8 interactors |
IntActi | Q8LB17, 8 interactors |
STRINGi | 3702.AT3G58460.2 |
PTM databases
iPTMneti | Q8LB17 |
Proteomic databases
PaxDbi | Q8LB17 |
ProteomicsDBi | 236512 [Q8LB17-1] |
Genome annotation databases
EnsemblPlantsi | AT3G58460.1; AT3G58460.1; AT3G58460 [Q8LB17-1] |
GeneIDi | 825015 |
Gramenei | AT3G58460.1; AT3G58460.1; AT3G58460 [Q8LB17-1] |
KEGGi | ath:AT3G58460 |
Organism-specific databases
Araporti | AT3G58460 |
Phylogenomic databases
eggNOGi | KOG2632, Eukaryota |
HOGENOMi | CLU_036445_1_0_1 |
InParanoidi | Q8LB17 |
OMAi | QWWENIP |
PhylomeDBi | Q8LB17 |
Miscellaneous databases
EvolutionaryTracei | Q8LB17 |
PROi | PR:Q8LB17 |
Gene expression databases
ExpressionAtlasi | Q8LB17, baseline and differential |
Genevisiblei | Q8LB17, AT |
Family and domain databases
Gene3Di | 1.20.1540.10, 1 hit |
InterProi | View protein in InterPro IPR022764, Peptidase_S54_rhomboid_dom IPR035952, Rhomboid-like_sf IPR015940, UBA IPR009060, UBA-like_sf |
Pfami | View protein in Pfam PF01694, Rhomboid, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00165, UBA, 1 hit |
SUPFAMi | SSF144091, SSF144091, 1 hit SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS50030, UBA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBL15_ARATH | |
Accessioni | Q8LB17Primary (citable) accession number: Q8LB17 Secondary accession number(s): C0Z2U1, Q8L7A1, Q9M2H0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | August 30, 2005 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families