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Protein

S-formylglutathione hydrolase

Gene

SFGH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde.1 Publication

Miscellaneous

The conserved Cys-59, implicated in catalysis in cysteine hydrolases, lies in close proximity to the serine hydrolase triad, serving a gate-keeping function in regulating access to the active site via disulfide formation with glutathione.

Caution

Was originally classified as an esterase D due to its apparent insensitivity to serine hydrolase inhibitors.1 Publication

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.

Activity regulationi

Activity toward p-nitrophenyl acetate inhibited by N-ethylmaleimide, 10-(fluoroethoxyphosphinyl)-N-(biotinamidopentyl)decanamide (FP-biotin), iodoacetamide, CuCl2 and ZnSO4, but not by phenylmethylsulfonyl fluoride, EDTA, Mg2+, Mn2+, Ca2+ or paraoxon, an organo-phosphate inhibitor of serine hydrolases.1 Publication

Kineticsi

  1. KM=0.13 mM for S-formylglutathione
  2. KM=0.15 mM for S-acetylglutathione
  3. KM=1.02 mM for p-nitrophenyl acetate
  4. KM=0.57 mM for alpha-naphthyl acetate
  5. KM=0.54 mM for beta-naphthyl acetate
  6. KM=0.03 mM for fluorescein diacetate
  7. KM=0.12 mM for 4-methylumbelliferyl acetate
  1. Vmax=219 nmol/sec/mg enzyme for S-formylglutathione
  2. Vmax=311 nmol/sec/mg enzyme for S-acetylglutathione
  3. Vmax=185 nmol/sec/mg enzyme for p-nitrophenyl acetate
  4. Vmax=350 nmol/sec/mg enzyme for alpha-naphthyl acetate
  5. Vmax=22 nmol/sec/mg enzyme for beta-naphthyl acetate
  6. Vmax=3 nmol/sec/mg enzyme for fluorescein diacetate
  7. Vmax=714 nmol/sec/mg enzyme for 4-methylumbelliferyl acetate

pH dependencei

Optimum pH is 7.6-8 for carboxyesterase activity.

Temperature dependencei

Fully active up to 55 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63Substrate1
Binding sitei67Substrate1
Active sitei152Charge relay systemBy similarity1
Active sitei229Charge relay systemBy similarity1
Active sitei262Charge relay systemBy similarity1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • S-formylglutathione hydrolase activity Source: TAIR

GO - Biological processi

  • formaldehyde catabolic process Source: InterPro
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

BioCyciARA:AT2G41530-MONOMER
BRENDAi3.1.2.12 399
ReactomeiR-ATH-156590 Glutathione conjugation

Protein family/group databases

ESTHERiarath-SFGH A85-EsteraseD-FGH

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase (EC:3.1.2.12)
Short name:
AtSFGH
Alternative name(s):
Esterase D
Gene namesi
Name:SFGH
Ordered Locus Names:At2g41530
ORF Names:T32G6.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41530
TAIRilocus:2062729 AT2G41530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59C → S: Loss of catalysis regulation. 1 Publication1
Mutagenesisi152S → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002481562 – 284S-formylglutathione hydrolaseAdd BLAST283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8LAS8
PRIDEiQ8LAS8

PTM databases

iPTMnetiQ8LAS8

Expressioni

Gene expression databases

ExpressionAtlasiQ8LAS8 baseline and differential
GenevisibleiQ8LAS8 AT

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4088, 2 interactors
IntActiQ8LAS8, 1 interactor
STRINGi3702.AT2G41530.1

Structurei

3D structure databases

ProteinModelPortaliQ8LAS8
SMRiQ8LAS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

eggNOGiKOG3101 Eukaryota
COG0627 LUCA
HOGENOMiHOG000263929
InParanoidiQ8LAS8
KOiK01070
OMAiEDHLRHH
OrthoDBiEOG09360HPP
PhylomeDBiQ8LAS8

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR014186 S-formylglutathione_hydrol
PANTHERiPTHR10061 PTHR10061, 1 hit
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR02821 fghA_ester_D, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LAS8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGLSEIGS TKMFDGYNKR YKHFSETLGC SMTFSIYFPP SASSSHKSPV
60 70 80 90 100
LYWLSGLTCT DENFIIKSGA QRAASTHGIA LVAPDTSPRG LNVEGEADSY
110 120 130 140 150
DFGVGAGFYL NATQEKWKNW RMYDYVVKEL PKLLSENFSQ LDTTKASISG
160 170 180 190 200
HSMGGHGALT IYLRNLDKYK SVSAFAPITN PINCAWGQKA FTNYLGDNKA
210 220 230 240 250
AWEEYDATCL ISKYNNLSAT ILIDQGENDQ FYPDQLLPSK FEEACKKVNA
260 270 280
PLLLRLHPGY DHSYYFIATF IEDHISHHAQ ALEL
Length:284
Mass (Da):31,656
Last modified:September 5, 2006 - v2
Checksum:i44F69C31F14BAC29
GO

Sequence cautioni

The sequence AAM65175 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3S → N in AAM65175 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347732 mRNA Translation: CAC87877.1
AC002510 Genomic DNA Translation: AAB84335.1
CP002685 Genomic DNA Translation: AEC09995.1
AY044322 mRNA Translation: AAK73263.1
AF446861 mRNA Translation: AAL38594.1
AF378875 mRNA Translation: AAK55678.1
AY087636 mRNA Translation: AAM65175.1 Different initiation.
PIRiT00809
RefSeqiNP_181684.1, NM_129716.4
UniGeneiAt.12399

Genome annotation databases

EnsemblPlantsiAT2G41530.1; AT2G41530.1; AT2G41530
GeneIDi818751
GrameneiAT2G41530.1; AT2G41530.1; AT2G41530
KEGGiath:AT2G41530

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347732 mRNA Translation: CAC87877.1
AC002510 Genomic DNA Translation: AAB84335.1
CP002685 Genomic DNA Translation: AEC09995.1
AY044322 mRNA Translation: AAK73263.1
AF446861 mRNA Translation: AAL38594.1
AF378875 mRNA Translation: AAK55678.1
AY087636 mRNA Translation: AAM65175.1 Different initiation.
PIRiT00809
RefSeqiNP_181684.1, NM_129716.4
UniGeneiAt.12399

3D structure databases

ProteinModelPortaliQ8LAS8
SMRiQ8LAS8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4088, 2 interactors
IntActiQ8LAS8, 1 interactor
STRINGi3702.AT2G41530.1

Protein family/group databases

ESTHERiarath-SFGH A85-EsteraseD-FGH

PTM databases

iPTMnetiQ8LAS8

Proteomic databases

PaxDbiQ8LAS8
PRIDEiQ8LAS8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41530.1; AT2G41530.1; AT2G41530
GeneIDi818751
GrameneiAT2G41530.1; AT2G41530.1; AT2G41530
KEGGiath:AT2G41530

Organism-specific databases

AraportiAT2G41530
TAIRilocus:2062729 AT2G41530

Phylogenomic databases

eggNOGiKOG3101 Eukaryota
COG0627 LUCA
HOGENOMiHOG000263929
InParanoidiQ8LAS8
KOiK01070
OMAiEDHLRHH
OrthoDBiEOG09360HPP
PhylomeDBiQ8LAS8

Enzyme and pathway databases

BioCyciARA:AT2G41530-MONOMER
BRENDAi3.1.2.12 399
ReactomeiR-ATH-156590 Glutathione conjugation

Miscellaneous databases

PROiPR:Q8LAS8

Gene expression databases

ExpressionAtlasiQ8LAS8 baseline and differential
GenevisibleiQ8LAS8 AT

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR014186 S-formylglutathione_hydrol
PANTHERiPTHR10061 PTHR10061, 1 hit
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR02821 fghA_ester_D, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSFGH_ARATH
AccessioniPrimary (citable) accession number: Q8LAS8
Secondary accession number(s): O22215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: September 12, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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