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UniProtKB - Q8L7W8 (FUCO2_ARATH)
Protein
Alpha-L-fucosidase 2
Gene
FUC95A
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. No activity with 3-fucosyllactose, p-nitrophenyl-alpha-I-fucopyranoside, lacto-N-fucopentaose II, lacto-N-fucopentaose III or alpha 1,6-fucosylated chitopentaose. Involved in apoplastic xyloglucan metabolism.
2 PublicationsCatalytic activityi
- EC:3.2.1.511 Publication
Kineticsi
- KM=0.65 mM for 2-fucosyllactose1 Publication
- KM=1.5 mM for polymeric xyloglucan1 Publication
pH dependencei
Optimum pH is 5.0.1 Publication
GO - Molecular functioni
- 1,2-alpha-L-fucosidase activity Source: TAIR
- alpha-L-fucosidase activity Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | ARA:AT4G34260-MONOMER |
Protein family/group databases
CAZyi | GH95, Glycoside Hydrolase Family 95 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-L-fucosidase 2 (EC:3.2.1.51)Alternative name(s): Alpha-1,2-fucosidase 2 Alpha-L-fucosidase 95A Short name: AtFuc95A Alpha-L-fucoside fucohydrolase 2 Protein ALTERED XYLOGLUCAN 8 |
Gene namesi | Name:FUC95A Synonyms:AXY8 Ordered Locus Names:At4g34260 ORF Names:F10M10.30 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G34260 |
TAIRi | locus:2116154, AT4G34260 |
Subcellular locationi
Extracellular region or secreted
- apoplast 1 Publication
Extracellular region or secreted
- apoplast Source: TAIR
Keywords - Cellular componenti
Apoplast, SecretedPathology & Biotechi
Disruption phenotypei
No visible phenotype. Higher abundance of fucosylated oligosaccharides and presence of unusual oligosaccharides.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 511 | C → Y in axy8-4; Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 562 | P → L in axy8-3; Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
ChainiPRO_0000289877 | 28 – 843 | Alpha-L-fucosidase 2Add BLAST | 816 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 62 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 253 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 365 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 605 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q8L7W8 |
PRIDEi | Q8L7W8 |
ProteomicsDBi | 230554 |
Expressioni
Tissue specificityi
Ubiquitous. Highest expression in vascular tissues, leaf trichomes, root elongation zone and emerging lateral roots.1 Publication
Gene expression databases
ExpressionAtlasi | Q8L7W8, baseline and differential |
Genevisiblei | Q8L7W8, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 95 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QQ9E, Eukaryota |
HOGENOMi | CLU_004617_2_2_1 |
InParanoidi | Q8L7W8 |
OMAi | MGAAWLC |
OrthoDBi | 614028at2759 |
PhylomeDBi | Q8L7W8 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR016518, Alpha-L-fucosidase IPR027414, GH95_N_dom |
Pfami | View protein in Pfam PF14498, Glyco_hyd_65N_2, 1 hit |
PIRSFi | PIRSF007663, UCP007663, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8L7W8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEKSSFFVH FSCLLLLLTI IITCGEGVRN PVRPRSSERR ALMDGQDLSR
60 70 80 90 100
PLKLTFGGPS RNWTDAIPIG NGRLGATIWG GVSSEILNIN EDTIWTGVPA
110 120 130 140 150
DYTNQKAPEA LAEVRRLVDE RNYAEATSEA VKLSGQPSDV YQIVGDLNLE
160 170 180 190 200
FDSSHRKYTQ ASYRRELDLE TAVAKVSYSV GAVDFSREFF ASNPDQVIIA
210 220 230 240 250
KIYASKPGSL SFKVSFDSEL HHHSETNPKA NQILMRGSCR PKRLPVNLKK
260 270 280 290 300
SINATNIPYD DHKGLQFASI LEVRVSNGGS VSSLGGKKLS VEKADWAVLL
310 320 330 340 350
LAASSNFDGP FTMPVDSKID PAKECVNRIS SVQKYSYSDL YARHLGDYQK
360 370 380 390 400
LFNRVSLHLS GSSTNETVQQ ATSTAERVRS FKTDQDPSLV ELLFQYGRYL
410 420 430 440 450
LISSSRPGTQ VANLQGIWNR DIQPPWDGAP HLNINLQMNY WHSLPGNIRE
460 470 480 490 500
CQEPLFDYMS ALAINGRKTA QVNYGASGWV AHQVSDIWAK TSPDRGEAVW
510 520 530 540 550
ALWPMGGAWL CTHAWEHYTY TMDKEFLKKK GYPLLEGCTS FLLDWLIKGK
560 570 580 590 600
DGFLQTNPST SPEHMFTAPI GKPASVSYSS TMDIAIIKEV FADIVSASEI
610 620 630 640 650
LGKTNDTLIG KVIAAQAKLP PTRISKDGSI REWAEDFEDP EVHHRHVSHL
660 670 680 690 700
FGLFPGHTIT VEKSPELAKA VEATLKKRGE EGPGWSTTWK AALWARLHNS
710 720 730 740 750
EHAYRMVTHI FDLVDPLNER NYEGGLYSNM FTAHPPFQID ANFGFAAAVA
760 770 780 790 800
EMLVQSTTKD LYLLPALPAD KWPNGIVNGL RARGGVTVSI KWMEGNLVEF
810 820 830 840
GLWSEQIVST RIVYRGISAA AELLPGKVFT FDKDLRCIRT DKL
Sequence cautioni
The sequence CAB36703 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80143 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL035521 Genomic DNA Translation: CAB36703.1 Sequence problems. AL161585 Genomic DNA Translation: CAB80143.1 Sequence problems. CP002687 Genomic DNA Translation: AEE86349.1 AY125494 mRNA Translation: AAM78086.1 BT002722 mRNA Translation: AAO11638.1 |
PIRi | T04772 |
RefSeqi | NP_195152.2, NM_119590.4 |
Genome annotation databases
EnsemblPlantsi | AT4G34260.1; AT4G34260.1; AT4G34260 |
GeneIDi | 829575 |
Gramenei | AT4G34260.1; AT4G34260.1; AT4G34260 |
KEGGi | ath:AT4G34260 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL035521 Genomic DNA Translation: CAB36703.1 Sequence problems. AL161585 Genomic DNA Translation: CAB80143.1 Sequence problems. CP002687 Genomic DNA Translation: AEE86349.1 AY125494 mRNA Translation: AAM78086.1 BT002722 mRNA Translation: AAO11638.1 |
PIRi | T04772 |
RefSeqi | NP_195152.2, NM_119590.4 |
3D structure databases
SMRi | Q8L7W8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G34260.1 |
Protein family/group databases
CAZyi | GH95, Glycoside Hydrolase Family 95 |
Proteomic databases
PaxDbi | Q8L7W8 |
PRIDEi | Q8L7W8 |
ProteomicsDBi | 230554 |
Genome annotation databases
EnsemblPlantsi | AT4G34260.1; AT4G34260.1; AT4G34260 |
GeneIDi | 829575 |
Gramenei | AT4G34260.1; AT4G34260.1; AT4G34260 |
KEGGi | ath:AT4G34260 |
Organism-specific databases
Araporti | AT4G34260 |
TAIRi | locus:2116154, AT4G34260 |
Phylogenomic databases
eggNOGi | ENOG502QQ9E, Eukaryota |
HOGENOMi | CLU_004617_2_2_1 |
InParanoidi | Q8L7W8 |
OMAi | MGAAWLC |
OrthoDBi | 614028at2759 |
PhylomeDBi | Q8L7W8 |
Enzyme and pathway databases
BioCyci | ARA:AT4G34260-MONOMER |
Miscellaneous databases
PROi | PR:Q8L7W8 |
Gene expression databases
ExpressionAtlasi | Q8L7W8, baseline and differential |
Genevisiblei | Q8L7W8, AT |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR016518, Alpha-L-fucosidase IPR027414, GH95_N_dom |
Pfami | View protein in Pfam PF14498, Glyco_hyd_65N_2, 1 hit |
PIRSFi | PIRSF007663, UCP007663, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FUCO2_ARATH | |
Accessioni | Q8L7W8Primary (citable) accession number: Q8L7W8 Secondary accession number(s): Q9SYZ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | September 29, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families