UniProtKB - Q8L7W5 (SMO11_ARATH)
Methylsterol monooxygenase 1-1
SMO1-1
Functioni
Non-heme iron oxygenase involved in sterols biosynthesis by catalyzing the removal of the first methyl group at the C-4 position (PubMed:14653780).
4,4-dimethyl-9-beta,19-cyclopropylsterols such as 24-methylenecycloartanol are the preferred substrates (PubMed:14653780).
Acts as a rate-limiting enzyme in the sterol pathway via interaction with ACBP1; sterols serve as lipid modulators for gene expression of homeodomain-leucine zipper IV transcription factors (PubMed:28500265).
Together with SMO1-2, involved in the maintenance of sterol composition to balance auxin and cytokinin activities during embryogenesis (PubMed:31341004).
3 PublicationsMiscellaneous
Catalytic activityi
- 4,4-dimethyl-5α-cholest-7-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β-methyl-5α-cholest-7-ene-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2O1 PublicationEC:1.14.18.91 Publication
- 24-methylenecycloartanol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β,14α-dimethyl-9β,19-cyclo-5α-ergost-24(241)-en-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2O1 PublicationEC:1.14.18.101 Publication
Cofactori
GO - Molecular functioni
- C-4 methylsterol oxidase activity Source: TAIR
- iron ion binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- 4,4-dimethyl-9beta,19-cyclopropylsterol oxidation Source: TAIR
- sterol biosynthetic process Source: TAIR
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Iron |
Enzyme and pathway databases
BioCyci | ARA:AT4G12110-MONOMER |
BRENDAi | 1.14.18.10, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Methylsterol monooxygenase 1-11 Publication (EC:1.14.18.101 Publication, EC:1.14.18.91 Publication)Alternative name(s): Sterol 4-alpha-methyl-oxidase 1-11 Publication Short name: AtSMO1-11 Publication |
Gene namesi | Name:SMO1-11 Publication Ordered Locus Names:At4g12110Imported ORF Names:F16J13.180Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G12110 |
TAIRi | locus:2118121, AT4G12110 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 42 – 62 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 96 – 116 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 118 – 138 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413161 | 1 – 298 | Methylsterol monooxygenase 1-1Add BLAST | 298 |
Proteomic databases
PaxDbi | Q8L7W5 |
PRIDEi | Q8L7W5 |
ProteomicsDBi | 228445 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
ExpressionAtlasi | Q8L7W5, baseline and differential |
Genevisiblei | Q8L7W5, AT |
Interactioni
Subunit structurei
Interacts with ACBP1.
1 PublicationProtein-protein interaction databases
STRINGi | 3702.AT4G12110.1 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 132 – 267 | Fatty acid hydroxylaseSequence analysisAdd BLAST | 136 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 147 – 151 | Histidine box-1By similarity | 5 | |
Motifi | 160 – 164 | Histidine box-2By similarity | 5 | |
Motifi | 239 – 245 | Histidine box-3By similarity | 7 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0873, Eukaryota |
HOGENOMi | CLU_047036_5_3_1 |
InParanoidi | Q8L7W5 |
OMAi | YGGVAHH |
OrthoDBi | 1493916at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MIPYATVEEA SIALGRNLTR LETLWFDYSA TKSDYYLYCH NILFLFLVFS
60 70 80 90 100
LVPLPLVFVE LARSASGLFN RYKIQPKVNY SLSDMFKCYK DVMTMFILVV
110 120 130 140 150
GPLQLVSYPS IQMIEIRSGL PLPTITEMLS QLVVYFLIED YTNYWVHRFF
160 170 180 190 200
HSKWGYDKIH RVHHEYTAPI GYAAPYAHWA EVLLLGIPTF MGPAIAPGHM
210 220 230 240 250
ITFWLWIALR QMEAIETHSG YDFPWSPTKY IPFYGGAEYH DYHHYVGGQS
260 270 280 290
QSNFASVFTY CDYIYGTDKG YRFQKKLLEQ IKESSKKSNK HNGGIKSD
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 59 | V → I in AAM64961 (Ref. 5) Curated | 1 | |
Sequence conflicti | 140 | D → E in AAQ13424 (PubMed:14653780).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF039199 mRNA Translation: AAQ13424.1 AL049638 Genomic DNA Translation: CAB40952.1 Sequence problems. AL161533 Genomic DNA Translation: CAB78254.1 Sequence problems. CP002687 Genomic DNA Translation: AEE83097.1 AY125499 mRNA Translation: AAM78091.1 BT000546 mRNA Translation: AAN18115.1 AY087412 mRNA Translation: AAM64961.1 |
PIRi | T06618 |
RefSeqi | NP_192948.1, NM_117281.3 |
Genome annotation databases
EnsemblPlantsi | AT4G12110.1; AT4G12110.1; AT4G12110 |
GeneIDi | 826819 |
Gramenei | AT4G12110.1; AT4G12110.1; AT4G12110 |
KEGGi | ath:AT4G12110 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF039199 mRNA Translation: AAQ13424.1 AL049638 Genomic DNA Translation: CAB40952.1 Sequence problems. AL161533 Genomic DNA Translation: CAB78254.1 Sequence problems. CP002687 Genomic DNA Translation: AEE83097.1 AY125499 mRNA Translation: AAM78091.1 BT000546 mRNA Translation: AAN18115.1 AY087412 mRNA Translation: AAM64961.1 |
PIRi | T06618 |
RefSeqi | NP_192948.1, NM_117281.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 3702.AT4G12110.1 |
Proteomic databases
PaxDbi | Q8L7W5 |
PRIDEi | Q8L7W5 |
ProteomicsDBi | 228445 |
Genome annotation databases
EnsemblPlantsi | AT4G12110.1; AT4G12110.1; AT4G12110 |
GeneIDi | 826819 |
Gramenei | AT4G12110.1; AT4G12110.1; AT4G12110 |
KEGGi | ath:AT4G12110 |
Organism-specific databases
Araporti | AT4G12110 |
TAIRi | locus:2118121, AT4G12110 |
Phylogenomic databases
eggNOGi | KOG0873, Eukaryota |
HOGENOMi | CLU_047036_5_3_1 |
InParanoidi | Q8L7W5 |
OMAi | YGGVAHH |
OrthoDBi | 1493916at2759 |
Enzyme and pathway databases
BioCyci | ARA:AT4G12110-MONOMER |
BRENDAi | 1.14.18.10, 399 |
Miscellaneous databases
PROi | PR:Q8L7W5 |
Gene expression databases
ExpressionAtlasi | Q8L7W5, baseline and differential |
Genevisiblei | Q8L7W5, AT |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMO11_ARATH | |
Accessioni | Q8L7W5Primary (citable) accession number: Q8L7W5 Secondary accession number(s): Q71V05, Q8LB57, Q9SZ76 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families