Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoamylase 2, chloroplastic

Gene

ISA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.3 Publications

Caution

Amino acids thought to be required for catalysis are not conserved in ISA2, suggesting that it may not be an active debranching enzyme and acts via its interaction with ISA1.Curated

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • isoamylase activity Source: TAIR

GO - Biological processi

  • amylopectin biosynthetic process Source: TAIR
  • starch biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Biological processCarbohydrate metabolism, Starch biosynthesis

Enzyme and pathway databases

BioCyciARA:AT1G03310-MONOMER
ARA:GQT-2133-MONOMER
MetaCyc:AT1G03310-MONOMER
BRENDAi3.2.1.68 399
UniPathwayi
UPA00152

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase 2, chloroplastic
Short name:
AtISA2
Alternative name(s):
Protein DEBRANCHING ENZYME 1
Gene namesi
Name:ISA2
Synonyms:DBE1
Ordered Locus Names:At1g03310
ORF Names:F15K9.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03310
TAIRilocus:2014500 AT1G03310

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Strong reduction of the starch level in leaves, but 50-fold increase of water-soluble polysaccharides. No alteration of the amylase-to-amylopectin ratio.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastCuratedAdd BLAST70
ChainiPRO_000037952871 – 882Isoamylase 2, chloroplasticAdd BLAST812

Proteomic databases

PaxDbiQ8L735
PRIDEiQ8L735

Expressioni

Gene expression databases

ExpressionAtlasiQ8L735 baseline and differential
GenevisibleiQ8L735 AT

Interactioni

Subunit structurei

Associates with ISA1 to form the heteromultimeric complex Iso1 required for amylopectin synthesis.

Protein-protein interaction databases

STRINGi3702.AT1G03310.1

Structurei

3D structure databases

ProteinModelPortaliQ8L735
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi788 – 793Poly-Glu6

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N Eukaryota
COG1523 LUCA
HOGENOMiHOG000239197
InParanoidiQ8L735
KOiK01214
OMAiWHVSLAS
OrthoDBiEOG093602VB
PhylomeDBiQ8L735

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02922 CBM_48, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L735-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAWSPSVGI GSCCLNNGIT RTWKFPSARL FTGRKNKIKL GSETLMFTRK
60 70 80 90 100
RFMGDLVTSA LQSYQFSKIC ASKTSIELRE ALSSRRAEAD DLKKVTSYSF
110 120 130 140 150
RTKAGALVKV KVEKKREKYS ILVYVSSLEL SGDDKSRLVM VWGVYRSDSS
160 170 180 190 200
CFLPLDFENS SQDSQTHTTE TTFVKSSLSE LMLGLEFDGK ESPFYLSFHL
210 220 230 240 250
KLVSGRDPDG QEMLTHRDTD FCIPVGFTAG HPLPLGLSSG PDDDSWNFSF
260 270 280 290 300
FSRSSTNVVL CLYDDSTTDK PALELDLDPY VNRTGDVWHA SVDNTWDFVR
310 320 330 340 350
YGYRCKETAH SKEDVDVEGE PIVLDPYATV VGKSVSQKYL GSLSKSPSFD
360 370 380 390 400
WGEDVSPNIP LEKLLVYRLN VKGFTQHRSS KLPSNVAGTF SGVAEKVSHL
410 420 430 440 450
KTLGTNAVLL EPIFSFSEQK GPYFPFHFFS PMDIYGPSNS LESAVNSMKV
460 470 480 490 500
MVKKLHSEGI EVLLEVVFTH TADSGALRGI DDSSYYYKGR ANDLDSKSYL
510 520 530 540 550
NCNYPVVQQL VLESLRYWVT EFHVDGFCFI NASSLLRGVH GEQLSRPPLV
560 570 580 590 600
EAIAFDPLLA ETKLIADCWD PLEMMPKEVR FPHWKRWAEL NTRYCRNVRN
610 620 630 640 650
FLRGRGVLSD LATRICGSGD VFTDGRGPAF SFNYISRNSG LSLVDIVSFS
660 670 680 690 700
GPELASELSW NCGEEGATNK SAVLQRRLKQ IRNFLFIQYI SLGVPVLNMG
710 720 730 740 750
DECGISTRGS PLLESRKPFD WNLLASAFGT QITQFISFMT SVRARRSDVF
760 770 780 790 800
QRRDFLKPEN IVWYANDQTT PKWEDPASKF LALEIKSESE EEETASLAEP
810 820 830 840 850
NEPKSNDLFI GFNASDHPES VVLPSLPDGS KWRRLVDTAL PFPGFFSVEG
860 870 880
ETVVAEEPLQ QLVVYEMKPY SCTLFETINT TA
Length:882
Mass (Da):98,884
Last modified:July 7, 2009 - v2
Checksum:i277940176F03B06D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211Q → R in AAM98123 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005278 Genomic DNA Translation: AAC72113.1
CP002684 Genomic DNA Translation: AEE27557.1
CP002684 Genomic DNA Translation: AEE27558.1
AY139980 mRNA Translation: AAM98123.1
PIRiF86164
RefSeqiNP_171830.1, NM_100213.4
NP_973751.1, NM_202022.3
UniGeneiAt.24041

Genome annotation databases

EnsemblPlantsiAT1G03310.1; AT1G03310.1; AT1G03310
AT1G03310.2; AT1G03310.2; AT1G03310
GeneIDi839531
GrameneiAT1G03310.1; AT1G03310.1; AT1G03310
AT1G03310.2; AT1G03310.2; AT1G03310
KEGGiath:AT1G03310

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005278 Genomic DNA Translation: AAC72113.1
CP002684 Genomic DNA Translation: AEE27557.1
CP002684 Genomic DNA Translation: AEE27558.1
AY139980 mRNA Translation: AAM98123.1
PIRiF86164
RefSeqiNP_171830.1, NM_100213.4
NP_973751.1, NM_202022.3
UniGeneiAt.24041

3D structure databases

ProteinModelPortaliQ8L735
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G03310.1

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Proteomic databases

PaxDbiQ8L735
PRIDEiQ8L735

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G03310.1; AT1G03310.1; AT1G03310
AT1G03310.2; AT1G03310.2; AT1G03310
GeneIDi839531
GrameneiAT1G03310.1; AT1G03310.1; AT1G03310
AT1G03310.2; AT1G03310.2; AT1G03310
KEGGiath:AT1G03310

Organism-specific databases

AraportiAT1G03310
TAIRilocus:2014500 AT1G03310

Phylogenomic databases

eggNOGiENOG410IN7N Eukaryota
COG1523 LUCA
HOGENOMiHOG000239197
InParanoidiQ8L735
KOiK01214
OMAiWHVSLAS
OrthoDBiEOG093602VB
PhylomeDBiQ8L735

Enzyme and pathway databases

UniPathwayi
UPA00152

BioCyciARA:AT1G03310-MONOMER
ARA:GQT-2133-MONOMER
MetaCyc:AT1G03310-MONOMER
BRENDAi3.2.1.68 399

Miscellaneous databases

PROiPR:Q8L735

Gene expression databases

ExpressionAtlasiQ8L735 baseline and differential
GenevisibleiQ8L735 AT

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02922 CBM_48, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISOA2_ARATH
AccessioniPrimary (citable) accession number: Q8L735
Secondary accession number(s): Q9ZVT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 7, 2009
Last modified: October 10, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again