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UniProtKB - Q8L164 (TREP_THEBR)

Entry version 52 (07 Oct 2020)
Sequence version 1 (01 Oct 2002)
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Protein

Alpha,alpha-trehalose phosphorylase

Gene

treP

Organism
Thermoanaerobacter brockii (Thermoanaerobium brockii)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible phosphorolytic cleavage of trehalose. Phosphorolysis is specific for trehalose, but D-xylose, D-galactose, L-arabinose, D-fucose, L-fucose, D-glucosamine and 2-deoxy D-glucose can act as substitutes for D-glucose in the synthetic reaction.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by Cu2+, Hg2+, Mg2+, Mn2+, Pb2+ and Zn2+.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 110 sec(-1) for trehalose. kcat is 110 sec(-1) for phosphate. kcat is 140 sec(-1) for D-glucose. kcat is 140 sec(-1) for beta-D-glucose 1-phosphate.
  1. KM=0.94 mM for trehalose1 Publication
  2. KM=0.57 mM for phosphate1 Publication
  3. KM=2.4 mM for D-glucose1 Publication
  4. KM=0.75 mM for beta-D-glucose 1-phosphate1 Publication

pH dependencei

Optimum pH is 7.0-7.5 for phosphorolysis, and 6.0-7.0 for synthetic reaction.1 Publication

Temperature dependencei

Optimum temperature is 70 degrees Celsius.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: trehalose degradation

This protein is involved in the pathway trehalose degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway trehalose degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei498Proton donorBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.4.1.64, 1463

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00300

Protein family/group databases

Carbohydrate-Active enZymes

More...CAZyi		GH65, Glycoside Hydrolase Family 65

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha,alpha-trehalose phosphorylase (EC:2.4.1.64)
Short name:
TPase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:treP
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiThermoanaerobacter brockii (Thermoanaerobium brockii)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri29323 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Thermoanaerobacter

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004189782 – 774Alpha,alpha-trehalose phosphorylaseAdd BLAST773

Proteomic databases

PRoteomics IDEntifications database

More...PRIDEi		Q8L164

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q8L164

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni369 – 370Substrate bindingBy similarity2
Regioni610 – 611Substrate bindingBy similarity2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyl hydrolase 65 family.Curated

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.50.10.10, 1 hit
2.70.98.40, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR008928, 6-hairpin_glycosidase_sf
IPR012341, 6hp_glycosidase-like_sf
IPR011013, Gal_mutarotase_sf_dom
IPR005194, Glyco_hydro_65_C
IPR005195, Glyco_hydro_65_M
IPR005196, Glyco_hydro_65_N
IPR037018, Glyco_hydro_65_N_sf
IPR017045, Malt_Pase/Glycosyl_Hdrlase

Pfam protein domain database

More...Pfami		View protein in Pfam
PF03633, Glyco_hydro_65C, 1 hit
PF03632, Glyco_hydro_65m, 1 hit
PF03636, Glyco_hydro_65N, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48208, SSF48208, 1 hit
SSF74650, SSF74650, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L164-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANKTKKPIY PFEDWVIRET QFSIDTNYRN ETIFTLANGY IGMRGTFEER 
        60         70         80         90        100
YSGPKNTSFN GTYINGFYEI HDIVYPEGGY GFAKIGQTML NVADSKIIKL 
       110        120        130        140        150
YVDGEEFDLL QGKILFYERV LDMKKGFVER KVKWESPTGK ILEVKIKRIV 
       160        170        180        190        200
SLNRQHLAAI SFTMQPVNFT GKIRFVSAID GNVSNINDSE DVRVGSNLKG 
       210        220        230        240        250
KVLKTIDKSV EGLKGWIVQK TQKSNFSYAC AIDNVLVADS KYEVSNSLEE 
       260        270        280        290        300
DGVKVIVDLE AEKGTSYTLN KFISYYTSKD FDENKLVALA LEEIEKAKND 
       310        320        330        340        350
GFETIEKEQE EFLNSFWKDA DVIIEGDKAL QQGIRFNEFH LLQSVGRDGK 
       360        370        380        390        400
TNIAAKGLTG GGYEGHYFWD SDIYIMPFFL YTKPEIAKAL VMYRYNLLDA 
       410        420        430        440        450
ARSRAKELGH KGALYPWRTI DGPECSAYFP AGTAQYHINA DIVYALKRYV 
       460        470        480        490        500
EATNDVDFLY DYGCEILFET ARFWEDLGAY IPLKGNKFCI NCVTGPDEYT 
       510        520        530        540        550
ALVDNNAYTN YMAKMNLEYA YDIANKMKKE VPQKYQKVAS KLNLKDEEIV 
       560        570        580        590        600
AWKKAADNMY LPYSKELDII PQDDSFLYKE RITVDEIPED QFPLLLHWHY 
       610        620        630        640        650
LNIYRYQICK QPDVLLLMFL QREKFTKDEL KKNYDYYEPI TTHDSSLSPA 
       660        670        680        690        700
IFSILANEIG YTDKAYKYFM MTARMDLDDY NDNVKDGIHA ASMAGTWSAV 
       710        720        730        740        750
VNGFGGMRVY TNELHFEPRL PKEWNLLSFN VRYKGRKINV KLTKENVVFA 
       760        770 
LLEGEPIEIY YFDKKILLEK GEIK
Length:774
Mass (Da):89,252
Last modified:October 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC136E8FEABE20E47
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AB073930 Genomic DNA Translation: BAB97299.1

Protein sequence database of the Protein Information Resource

More...PIRi		JC7887

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073930 Genomic DNA Translation: BAB97299.1
PIRiJC7887

3D structure databases

SMRiQ8L164
ModBaseiSearch...

Protein family/group databases

CAZyiGH65, Glycoside Hydrolase Family 65

Proteomic databases

PRIDEiQ8L164

Enzyme and pathway databases

UniPathwayiUPA00300
BRENDAi2.4.1.64, 1463

Family and domain databases

Gene3Di1.50.10.10, 1 hit
2.70.98.40, 1 hit
InterProiView protein in InterPro
IPR008928, 6-hairpin_glycosidase_sf
IPR012341, 6hp_glycosidase-like_sf
IPR011013, Gal_mutarotase_sf_dom
IPR005194, Glyco_hydro_65_C
IPR005195, Glyco_hydro_65_M
IPR005196, Glyco_hydro_65_N
IPR037018, Glyco_hydro_65_N_sf
IPR017045, Malt_Pase/Glycosyl_Hdrlase
PfamiView protein in Pfam
PF03633, Glyco_hydro_65C, 1 hit
PF03632, Glyco_hydro_65m, 1 hit
PF03636, Glyco_hydro_65N, 1 hit
PIRSFiPIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit
SUPFAMiSSF48208, SSF48208, 1 hit
SSF74650, SSF74650, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTREP_THEBR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8L164
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2002
Last modified: October 7, 2020
This is version 52 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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