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UniProtKB - Q8L164 (TREP_THEBR)
Protein
Alpha,alpha-trehalose phosphorylase
Gene
treP
Organism
Thermoanaerobacter brockii (Thermoanaerobium brockii)
Status
Functioni
Catalyzes the reversible phosphorolytic cleavage of trehalose. Phosphorolysis is specific for trehalose, but D-xylose, D-galactose, L-arabinose, D-fucose, L-fucose, D-glucosamine and 2-deoxy D-glucose can act as substitutes for D-glucose in the synthetic reaction.
1 PublicationCatalytic activityi
- EC:2.4.1.641 Publication
Activity regulationi
Inhibited by Cu2+, Hg2+, Mg2+, Mn2+, Pb2+ and Zn2+.1 Publication
Kineticsi
kcat is 110 sec(-1) for trehalose. kcat is 110 sec(-1) for phosphate. kcat is 140 sec(-1) for D-glucose. kcat is 140 sec(-1) for beta-D-glucose 1-phosphate.
- KM=0.94 mM for trehalose1 Publication
- KM=0.57 mM for phosphate1 Publication
- KM=2.4 mM for D-glucose1 Publication
- KM=0.75 mM for beta-D-glucose 1-phosphate1 Publication
pH dependencei
Optimum pH is 7.0-7.5 for phosphorolysis, and 6.0-7.0 for synthetic reaction.1 Publication
Temperature dependencei
Optimum temperature is 70 degrees Celsius.1 Publication
: trehalose degradation Pathwayi
This protein is involved in the pathway trehalose degradation, which is part of Glycan degradation.View all proteins of this organism that are known to be involved in the pathway trehalose degradation and in Glycan degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 498 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- alpha,alpha-trehalose phosphorylase activity Source: UniProtKB
- carbohydrate binding Source: InterPro
GO - Biological processi
- trehalose catabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.64, 1463 |
UniPathwayi | UPA00300 |
Protein family/group databases
CAZyi | GH65, Glycoside Hydrolase Family 65 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:treP |
Organismi | Thermoanaerobacter brockii (Thermoanaerobium brockii) |
Taxonomic identifieri | 29323 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Thermoanaerobacter |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000418978 | 2 – 774 | Alpha,alpha-trehalose phosphorylaseAdd BLAST | 773 |
Proteomic databases
PRIDEi | Q8L164 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 369 – 370 | Substrate bindingBy similarity | 2 | |
Regioni | 610 – 611 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 65 family.Curated
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit 2.70.98.40, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR011013, Gal_mutarotase_sf_dom IPR005194, Glyco_hydro_65_C IPR005195, Glyco_hydro_65_M IPR005196, Glyco_hydro_65_N IPR037018, Glyco_hydro_65_N_sf IPR017045, Malt_Pase/Glycosyl_Hdrlase |
Pfami | View protein in Pfam PF03633, Glyco_hydro_65C, 1 hit PF03632, Glyco_hydro_65m, 1 hit PF03636, Glyco_hydro_65N, 1 hit |
PIRSFi | PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF74650, SSF74650, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8L164-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANKTKKPIY PFEDWVIRET QFSIDTNYRN ETIFTLANGY IGMRGTFEER
60 70 80 90 100
YSGPKNTSFN GTYINGFYEI HDIVYPEGGY GFAKIGQTML NVADSKIIKL
110 120 130 140 150
YVDGEEFDLL QGKILFYERV LDMKKGFVER KVKWESPTGK ILEVKIKRIV
160 170 180 190 200
SLNRQHLAAI SFTMQPVNFT GKIRFVSAID GNVSNINDSE DVRVGSNLKG
210 220 230 240 250
KVLKTIDKSV EGLKGWIVQK TQKSNFSYAC AIDNVLVADS KYEVSNSLEE
260 270 280 290 300
DGVKVIVDLE AEKGTSYTLN KFISYYTSKD FDENKLVALA LEEIEKAKND
310 320 330 340 350
GFETIEKEQE EFLNSFWKDA DVIIEGDKAL QQGIRFNEFH LLQSVGRDGK
360 370 380 390 400
TNIAAKGLTG GGYEGHYFWD SDIYIMPFFL YTKPEIAKAL VMYRYNLLDA
410 420 430 440 450
ARSRAKELGH KGALYPWRTI DGPECSAYFP AGTAQYHINA DIVYALKRYV
460 470 480 490 500
EATNDVDFLY DYGCEILFET ARFWEDLGAY IPLKGNKFCI NCVTGPDEYT
510 520 530 540 550
ALVDNNAYTN YMAKMNLEYA YDIANKMKKE VPQKYQKVAS KLNLKDEEIV
560 570 580 590 600
AWKKAADNMY LPYSKELDII PQDDSFLYKE RITVDEIPED QFPLLLHWHY
610 620 630 640 650
LNIYRYQICK QPDVLLLMFL QREKFTKDEL KKNYDYYEPI TTHDSSLSPA
660 670 680 690 700
IFSILANEIG YTDKAYKYFM MTARMDLDDY NDNVKDGIHA ASMAGTWSAV
710 720 730 740 750
VNGFGGMRVY TNELHFEPRL PKEWNLLSFN VRYKGRKINV KLTKENVVFA
760 770
LLEGEPIEIY YFDKKILLEK GEIK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB073930 Genomic DNA Translation: BAB97299.1 |
PIRi | JC7887 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB073930 Genomic DNA Translation: BAB97299.1 |
PIRi | JC7887 |
3D structure databases
SMRi | Q8L164 |
ModBasei | Search... |
Protein family/group databases
CAZyi | GH65, Glycoside Hydrolase Family 65 |
Proteomic databases
PRIDEi | Q8L164 |
Enzyme and pathway databases
UniPathwayi | UPA00300 |
BRENDAi | 2.4.1.64, 1463 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit 2.70.98.40, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR011013, Gal_mutarotase_sf_dom IPR005194, Glyco_hydro_65_C IPR005195, Glyco_hydro_65_M IPR005196, Glyco_hydro_65_N IPR037018, Glyco_hydro_65_N_sf IPR017045, Malt_Pase/Glycosyl_Hdrlase |
Pfami | View protein in Pfam PF03633, Glyco_hydro_65C, 1 hit PF03632, Glyco_hydro_65m, 1 hit PF03636, Glyco_hydro_65N, 1 hit |
PIRSFi | PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF74650, SSF74650, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TREP_THEBR | |
Accessioni | Q8L164Primary (citable) accession number: Q8L164 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | October 1, 2002 | |
Last modified: | October 7, 2020 | |
This is version 52 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families