Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Light-independent protochlorophyllide reductase subunit B

Gene

bchB

Organism
uncultured marine proteobacterium
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (BchN-BchB) is the catalytic component of the complex.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at the heterodimer interface by residues from both subunits.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation1
Active sitei274Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processBacteriochlorophyll biosynthesisUniRule annotation, Chlorophyll biosynthesis, PhotosynthesisUniRule annotation
Ligand4Fe-4SUniRule annotation, ATP-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00671

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase subunit BUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit BUniRule annotation
Short name:
LI-POR subunit BUniRule annotation
Gene namesi
Name:bchBUniRule annotationImported
ORF Names:MBMO_EBAC000-60D04.48Imported
Organismiuncultured marine proteobacteriumImported
Taxonomic identifieri482892 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaenvironmental samples

Interactioni

Subunit structurei

Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB. Forms a heterotetramer of two BchB and two BchN subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8KYZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 406Oxidored_nitroInterPro annotationAdd BLAST394
Domaini465 – 509PCP_redInterPro annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni409 – 410Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ChlB/BchB/BchZ family.UniRule annotation

Family and domain databases

HAMAPiMF_00353 ChlB_BchB, 1 hit
InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR013580 P-CP/CP_red_C
IPR005969 Protochl_reductB
IPR016209 Protochlorophyllide_Rdtase
PfamiView protein in Pfam
PF00148 Oxidored_nitro, 1 hit
PF08369 PCP_red, 1 hit
PIRSFiPIRSF000163 PCP_ChlB, 1 hit
TIGRFAMsiTIGR01278 DPOR_BchB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KYZ3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLTVWTYEG PPHVGAMRVA TGMKKLHYVL HAPQGDTYAD LLFTMIERRD
60 70 80 90 100
HRPPVTYTTF QARDLGADTA GLFKKACQDA YDRFQPEAII VGASCTAELI
110 120 130 140 150
QDDPGGLAET MGLPIPVIPL ELPSYQRKEN FGADETFFQL VKHLAKPQAK
160 170 180 190 200
TERVSCNIIG PTALGFRHRD DVAEITNLLF ELGIEVNVTA PMGATPSDIA
210 220 230 240 250
QMGKAHFNVL LYPETGETAA RYCEKEFDQP YTKIVPIGAG ATHDFITEVR
260 270 280 290 300
ALSGSTAPLD SQALRQTWWS RSVDSTYLTG KRVFIFGDGT HVKAAARIAR
310 320 330 340 350
DEMGFEVAGL GCYNREYARD IRTIAKEFGV EALITDDYLL VEKTIEALQP
360 370 380 390 400
EMILGTQMER HIGKRLGIPC AVISAPVHVQ DFPARYSPQM GFEGANVIFD
410 420 430 440 450
TWIHPLVMGL EEHLLHMFRE DFEFNDAAGP SHHGGHQPSG TASVQVPAAQ
460 470 480 490 500
EQSLALSTGG DVAIWLAEAE KELKKIPFFV RGKARRNTET YATSQGLSEI
510
SVDTLYEAKA FYAR
Length:514
Mass (Da):56,812
Last modified:October 1, 2002 - v1
Checksum:iF66AC8198341CA11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008921 Genomic DNA Translation: AAM48680.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008921 Genomic DNA Translation: AAM48680.1

3D structure databases

ProteinModelPortaliQ8KYZ3
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00671

Family and domain databases

HAMAPiMF_00353 ChlB_BchB, 1 hit
InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR013580 P-CP/CP_red_C
IPR005969 Protochl_reductB
IPR016209 Protochlorophyllide_Rdtase
PfamiView protein in Pfam
PF00148 Oxidored_nitro, 1 hit
PF08369 PCP_red, 1 hit
PIRSFiPIRSF000163 PCP_ChlB, 1 hit
TIGRFAMsiTIGR01278 DPOR_BchB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ8KYZ3_9PROT
AccessioniPrimary (citable) accession number: Q8KYZ3
Entry historyiIntegrated into UniProtKB/TrEMBL: October 1, 2002
Last sequence update: October 1, 2002
Last modified: September 27, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again