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UniProtKB - Q8KDS8 (AAPAT_CHLTE)
Protein
Aspartate/prephenate aminotransferase
Gene
CT0966
Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Functioni
Catalyzes the reversible conversion of aspartate and 2-oxoglutarate to glutamate and oxaloacetate (PubMed:25070637).
Can also transaminate prephenate in the presence of aspartate (PubMed:25070637).
1 PublicationCatalytic activityi
Cofactori
pyridoxal 5'-phosphate1 Publication
Kineticsi
kcat is 17.2 sec(-1) with 2-oxoglutarate as substrate. kcat is 12.3 sec(-1) with prephenate as substrate.1 Publication
- KM=949 µM for 2-oxoglutarate1 Publication
- KM=464 µM for prephenate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 18 | Important for prephenate aminotransferase activityBy similarity | 1 | |
Binding sitei | 45 | Aspartate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 131 | AspartateBy similarity | 1 | |
Binding sitei | 181 | AspartateBy similarity | 1 | |
Binding sitei | 378 | AspartateBy similarity | 1 |
GO - Molecular functioni
- aspartate-prephenate aminotransferase activity Source: UniProtKB-EC
- L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- biosynthetic process Source: InterPro
Keywordsi
Molecular function | Aminotransferase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | CTEP194439:G1FZE-1002-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:CT0966Imported |
Organismi | Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum) |
Taxonomic identifieri | 194439 [NCBI] |
Taxonomic lineagei | Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000448260 | 1 – 400 | Aspartate/prephenate aminotransferaseAdd BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 243 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 194439.CT0966 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | COG0436, Bacteria |
HOGENOMi | CLU_017584_4_3_10 |
OMAi | SVAMTGW |
OrthoDBi | 554560at2 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8KDS8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVESFERFL SRRVLSMQES QTMKITGLAK KMQAEGKDVV SLSAGEPDFP
60 70 80 90 100
TPENVCEAGI EAIRKGFTRY TANSGIPELK KAIIRKLQRD NGLEYAEDEI
110 120 130 140 150
IVSNGGKQAL ANTFLALCDE GDEVIVPAPY WVSFPEMARL AEATPVIVET
160 170 180 190 200
SIETGYKMTP EQLAAAITPK TRILVLNSPS NPSGAVYNEA EVRALMQVIE
210 220 230 240 250
GKEIFVLSDE MYDMICYGGV RPFSPARIPE MKPWVIVSNG TSKSYSMTGW
260 270 280 290 300
RIGYLAAPKW IINACDKIQS QTTSNANSIA QKAAVAALDG DQSIVEQRRA
310 320 330 340 350
EFEKRRDFMF RELNTISGIE CTLPEGAFYI FPSIKGLLGK TFGGKVMKDS
360 370 380 390 400
TDVAEYLLTE HYVATVPGDA FGAPENLRLS YAASIEELAE AVNRIRKAFS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006470 Genomic DNA Translation: AAM72201.1 |
RefSeqi | NP_661859.1, NC_002932.3 WP_010932646.1, NC_002932.3 |
Genome annotation databases
EnsemblBacteriai | AAM72201; AAM72201; CT0966 |
KEGGi | cte:CT0966 |
PATRICi | fig|194439.7.peg.876 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006470 Genomic DNA Translation: AAM72201.1 |
RefSeqi | NP_661859.1, NC_002932.3 WP_010932646.1, NC_002932.3 |
3D structure databases
SMRi | Q8KDS8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 194439.CT0966 |
Genome annotation databases
EnsemblBacteriai | AAM72201; AAM72201; CT0966 |
KEGGi | cte:CT0966 |
PATRICi | fig|194439.7.peg.876 |
Phylogenomic databases
eggNOGi | COG0436, Bacteria |
HOGENOMi | CLU_017584_4_3_10 |
OMAi | SVAMTGW |
OrthoDBi | 554560at2 |
Enzyme and pathway databases
BioCyci | CTEP194439:G1FZE-1002-MONOMER |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AAPAT_CHLTE | |
Accessioni | Q8KDS8Primary (citable) accession number: Q8KDS8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2019 |
Last sequence update: | October 1, 2002 | |
Last modified: | September 29, 2021 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families