UniProtKB - Q8K4Z2 (FNDC5_MOUSE)
Protein
Fibronectin type III domain-containing protein 5
Gene
Fndc5
Organism
Mus musculus (Mouse)
Status
Functioni
mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.1 Publication
GO - Molecular functioni
- hormone activity Source: UniProtKB-KW
GO - Biological processi
- positive regulation of brown fat cell differentiation Source: UniProtKB
- response to muscle activity Source: UniProtKB
Keywordsi
Molecular function | Hormone |
Names & Taxonomyi
Protein namesi | Recommended name: Fibronectin type III domain-containing protein 5Alternative name(s): Fibronectin type III repeat-containing protein 2 Peroxisomal protein Short name: PeP Cleaved into the following chain: |
Gene namesi | Name:Fndc5 Synonyms:Frcp2, Pep |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917614, Fndc5 |
Subcellular locationi
Plasma membrane
Extracellular region or secreted
Peroxisome
Note: Imported in peroxisomes through the PEX5 receptor pathway.
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB
Peroxisome
- peroxisomal membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 29 – 149 | ExtracellularSequence analysisAdd BLAST | 121 | |
Transmembranei | 150 – 170 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 171 – 209 | CytoplasmicSequence analysisAdd BLAST | 39 |
Keywords - Cellular componenti
Cell membrane, Membrane, Peroxisome, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | Sequence analysisAdd BLAST | 28 | |
ChainiPRO_0000328972 | 29 – 209 | Fibronectin type III domain-containing protein 5Add BLAST | 181 | |
ChainiPRO_0000415858 | 29 – 140 | IrisinAdd BLAST | 112 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 36 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 81 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.1 Publication
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q8K4Z2 |
PRIDEi | Q8K4Z2 |
PTM databases
GlyGeni | Q8K4Z2, 2 sites |
iPTMneti | Q8K4Z2 |
PhosphoSitePlusi | Q8K4Z2 |
Expressioni
Tissue specificityi
In adult, it is highly expressed in skeletal muscle, heart and brain.2 Publications
Developmental stagei
During embryonic development, it is detected almost exclusively in the skeletal muscle, and at lower level in brain. In skeletal muscle, it is induced after myoblast differentiation, when myotube formation is promoted.1 Publication
Inductioni
Up-regulated by muscular exercise. This effect can be mediated, at least partly, by PPARGC1A.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000001334, Expressed in interventricular septum and 209 other tissues |
Genevisiblei | Q8K4Z2, MM |
Interactioni
GO - Molecular functioni
- hormone activity Source: UniProtKB-KW
Protein-protein interaction databases
IntActi | Q8K4Z2, 1 interactor |
MINTi | Q8K4Z2 |
STRINGi | 10090.ENSMUSP00000099660 |
Miscellaneous databases
RNActi | Q8K4Z2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 124 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 92 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 207 – 209 | Microbody targeting signalSequence analysis | 3 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQCU, Eukaryota |
GeneTreei | ENSGT00390000004923 |
HOGENOMi | CLU_089166_1_0_1 |
InParanoidi | Q8K4Z2 |
OMAi | EMGMKSQ |
OrthoDBi | 1198350at2759 |
PhylomeDBi | Q8K4Z2 |
TreeFami | TF325415 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR032073, FNDC5_C IPR013783, Ig-like_fold |
Pfami | View protein in Pfam PF16066, DUF4808, 1 hit PF00041, fn3, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8K4Z2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPGPCAWPP RAALRLWLGC VCFALVQADS PSAPVNVTVR HLKANSAVVS
60 70 80 90 100
WDVLEDEVVI GFAISQQKKD VRMLRFIQEV NTTTRSCALW DLEEDTEYIV
110 120 130 140 150
HVQAISIQGQ SPASEPVLFK TPREAEKMAS KNKDEVTMKE MGRNQQLRTG
160 170 180 190 200
EVLIIVVVLF MWAGVIALFC RQYDIIKDNE PNNNKEKTKS ASETSTPEHQ
GGGLLRSKI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 – 14 | AL → VV in BAB23815 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 64 | I → L in BAB23815 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 124 | E → A in BAB23815 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 130 | S → P in BAB23815 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 158 | V → C in BAB23815 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 171 | R → A in BAB23815 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ401376 mRNA Translation: CAC82194.1 AK005096 mRNA Translation: BAB23815.1 AL606977 Genomic DNA No translation available. BC109183 mRNA Translation: AAI09184.1 BC109184 mRNA Translation: AAI09185.1 |
CCDSi | CCDS18683.1 |
RefSeqi | NP_081678.1, NM_027402.4 |
Genome annotation databases
Ensembli | ENSMUST00000102600; ENSMUSP00000099660; ENSMUSG00000001334 |
GeneIDi | 384061 |
KEGGi | mmu:384061 |
UCSCi | uc008uwe.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ401376 mRNA Translation: CAC82194.1 AK005096 mRNA Translation: BAB23815.1 AL606977 Genomic DNA No translation available. BC109183 mRNA Translation: AAI09184.1 BC109184 mRNA Translation: AAI09185.1 |
CCDSi | CCDS18683.1 |
RefSeqi | NP_081678.1, NM_027402.4 |
3D structure databases
SMRi | Q8K4Z2 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8K4Z2, 1 interactor |
MINTi | Q8K4Z2 |
STRINGi | 10090.ENSMUSP00000099660 |
PTM databases
GlyGeni | Q8K4Z2, 2 sites |
iPTMneti | Q8K4Z2 |
PhosphoSitePlusi | Q8K4Z2 |
Proteomic databases
PaxDbi | Q8K4Z2 |
PRIDEi | Q8K4Z2 |
Protocols and materials databases
Antibodypediai | 54866, 192 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000102600; ENSMUSP00000099660; ENSMUSG00000001334 |
GeneIDi | 384061 |
KEGGi | mmu:384061 |
UCSCi | uc008uwe.1, mouse |
Organism-specific databases
CTDi | 252995 |
MGIi | MGI:1917614, Fndc5 |
Phylogenomic databases
eggNOGi | ENOG502QQCU, Eukaryota |
GeneTreei | ENSGT00390000004923 |
HOGENOMi | CLU_089166_1_0_1 |
InParanoidi | Q8K4Z2 |
OMAi | EMGMKSQ |
OrthoDBi | 1198350at2759 |
PhylomeDBi | Q8K4Z2 |
TreeFami | TF325415 |
Miscellaneous databases
BioGRID-ORCSi | 384061, 1 hit in 17 CRISPR screens |
ChiTaRSi | Fndc5, mouse |
PROi | PR:Q8K4Z2 |
RNActi | Q8K4Z2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001334, Expressed in interventricular septum and 209 other tissues |
Genevisiblei | Q8K4Z2, MM |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR032073, FNDC5_C IPR013783, Ig-like_fold |
Pfami | View protein in Pfam PF16066, DUF4808, 1 hit PF00041, fn3, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FNDC5_MOUSE | |
Accessioni | Q8K4Z2Primary (citable) accession number: Q8K4Z2 Secondary accession number(s): Q9DB97 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot