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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Gene

Plce1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.4 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.1 Publication

Cofactori

Ca2+By similarity

Activity regulationi

Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1388PROSITE-ProRule annotation1
Active sitei1433PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor, Hydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.11 3474
ReactomeiR-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-epsilon-1
Phospholipase C-epsilon-1
Short name:
PLC-epsilon-1
Gene namesi
Name:Plce1
Synonyms:Kiaa1516, Plce
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1921305 Plce1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice exhibit delayed onset and markedly reduced incidence of chemically induced skin squamous tumors. They also display cardiac malformations which mainly affects aortic and pulmonary valves and enhanced susceptibility to cardiac hypertrophy and fibrosis in response to chronic stress.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002562391 – 22821-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1Add BLAST2282

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1093PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K4S1
PaxDbiQ8K4S1
PRIDEiQ8K4S1

PTM databases

iPTMnetiQ8K4S1
PhosphoSitePlusiQ8K4S1

Expressioni

Tissue specificityi

Highly expressed in neurons and to a lower extent in skin, skeletal muscle and heart (at protein level). Expressed in the epidermis.2 Publications

Developmental stagei

Specifically expressed in cells committed to the neuronal lineage (at protein level). Weakly expressed at E7, expression strongly increases at later embryonic stages. Expressed abundantly in almost all neural tissues at E12.5 and also detected in tongue muscles, genital tubercle and hand plate. At E15.5 a strong expression in skeletal muscles is detected together with the strong expression in neural tissues.1 Publication

Inductioni

Up-regulated during the differentiation of neural precursor cells into neurons but not glial cells. Up-regulated in heart upon induced hypertrophy.2 Publications

Gene expression databases

BgeeiENSMUSG00000024998 Expressed in 261 organ(s), highest expression level in stria vascularis of cochlear duct
CleanExiMM_PLCE1
ExpressionAtlasiQ8K4S1 baseline and differential
GenevisibleiQ8K4S1 MM

Interactioni

Subunit structurei

Interacts with GTP-bound HRAS, RAP1A, RAP2A, RAP2B and RHOA (By similarity). Interacts with IQGAP1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Ryr2E9Q4012EBI-6902760,EBI-643628

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60738N
IntActiQ8K4S1, 5 interactors
MINTiQ8K4S1
STRINGi10090.ENSMUSP00000130604

Structurei

3D structure databases

ProteinModelPortaliQ8K4S1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini528 – 781Ras-GEFPROSITE-ProRule annotationAdd BLAST254
Domaini1373 – 1521PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST149
Domaini1710 – 1826PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini1836 – 1936C2PROSITE-ProRule annotationAdd BLAST101
Domaini1992 – 2094Ras-associating 1PROSITE-ProRule annotationAdd BLAST103
Domaini2115 – 2218Ras-associating 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1667 – 1744Required for activation by RHOA, RHOB, GNA12, GNA13 and G-beta gammaBy similarityAdd BLAST78

Domaini

The Ras-associating domain 1 is degenerated and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane (By similarity).By similarity
The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00730000110782
HOVERGENiHBG059220
InParanoidiQ8K4S1
KOiK05860
TreeFamiTF314432

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR028398 PLC-epsilon1
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
IPR000159 RA_dom
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF6 PTHR10336:SF6, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF00788 RA, 1 hit
PF00617 RasGEF, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SM00147 RasGEF, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF48366 SSF48366, 1 hit
SSF51695 SSF51695, 1 hit
SSF54236 SSF54236, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
PS50200 RA, 1 hit
PS50009 RASGEF_CAT, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8K4S1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSEEMAASV LIPVTQRKVA SAQSVAEERS VKVSDAGIPR ARAGRQGALI
60 70 80 90 100
PPTISQWNKH KEESSRSDLS KVFSIARGEL VCDENSNEEG WEENAPDSPE
110 120 130 140 150
NHAMNGNSLV QSHQHQFPRS QLCEARDSVT EDPCLQPGIP SPLERKVLPG
160 170 180 190 200
IQLEMEDSPM DVSPAGSQPR IMESSGPHSD RNTAVFHFHY EADRTMSDAF
210 220 230 240 250
HTLSENLILD DCANCVTLPG GQQNKNCMAY ACKLVELTRT CGSKNGQVQC
260 270 280 290 300
EHCTSLRDEY LCFESSCSKA DEVCSGGGFC EDGFAHGPAA KTFLNPLEDF
310 320 330 340 350
SDNCEDVDDF FKSKKERSTL LVRRFCKNDR EVKKSVYTGT RAIMRTLPSG
360 370 380 390 400
CIGPAAWNYV DQKKAGLLWP CGNVMGTLSA MDIRQSGSQR LSEAQWCLIY
410 420 430 440 450
SAVRRGEEIE DTVGSLLHCS TQLPNSETAH GRIEDGPCLK QCVRDTECEF
460 470 480 490 500
RATLQRTSIA QYITGSLLEA TTSLGARSGL LSSFGGSTGR IMLKERQLGT
510 520 530 540 550
SMANSNPVPS SSAGISKELI DLQPLIQFPE EVASILTEQE QNIYRRVLPM
560 570 580 590 600
DYLCFLTRDL SSPECQRSLP RLKASISESI LTSQSGEHNA LEDLVMRFNE
610 620 630 640 650
VSSWVTWLIL TAGSMEEKRE VFSYLVHVAK CCWNMGNYNA VMEFLAGLRS
660 670 680 690 700
RKVLKMWQFM DQSDIETMRS LKDAMAQHES SVEYKKVVTR ALHIPGCKVV
710 720 730 740 750
PFCGVFLKEL CEVLDGASGL LKLCPRYSSQ EEALEFVADY SGQDNFLQRV
760 770 780 790 800
GQNGLKNSEK ELTVNSIFQV IRSCSRSLEM EEEDSASEGS GSRKNSLKDK
810 820 830 840 850
ARWQFIIGDL LDSENDIFEK SKECDPHGSE ESQKAFDHGT ELIPWYVLSI
860 870 880 890 900
QADVHQFLLQ GATVIHYDQD THLSARCFLQ LQPDNSTLTW MKPPTASPAG
910 920 930 940 950
ARPKLGVLSN MAEPGKFPSP GNAGVSGLAE GILDLFSVKA VYMGHPGIDI
960 970 980 990 1000
HTVCVQNKLS SMLLSETGVT LLYGLQTTDN RLLHFVAPKH TAEMLFSGLL
1010 1020 1030 1040 1050
ELTTAVRKIR RFPDQRQQWL RKQYVSLYQE DGRYEGPTLA HAVELFGGRR
1060 1070 1080 1090 1100
WSTRNPSPGM SAKNAEKPNM QRNNTLGIST TKKKKKMLMR GESGEVTDDE
1110 1120 1130 1140 1150
MATRKAKMYR ECRSRSGSDP QDVNEQEESE ANVITNPPNP LHSRRAYSLT
1160 1170 1180 1190 1200
TAGSPNLATG MSSPISAWSS SSWHGRIRGG MQGFQSFMVS DSNMSFVEFV
1210 1220 1230 1240 1250
ELFKSFSIRS RKDLKDIFDI YSVPCNRSAS ESAPLYTNLT IEENTSDLQP
1260 1270 1280 1290 1300
DLDLLTRNVS DLGLFIKSKQ QLSDNQRQIS DAIAAASIVT NGTGIESTSL
1310 1320 1330 1340 1350
GIFGVGILQL NDFLVNCQGE HCTYDEILSI IQKFEPSVSM CHQGLLSFEG
1360 1370 1380 1390 1400
FARFLMDKDN FASKNDESRE NKKELQLPLS YYYIESSHNT YLTGHQLKGE
1410 1420 1430 1440 1450
SSVELYSQVL LQGCRSIELD CWDGDDGMPI IYHGHTLTTK IPFKEVVEAI
1460 1470 1480 1490 1500
DRSAFITSDL PIIISIENHC SLPQQRKMAE IFKSVFGEKL VAKFLFETDF
1510 1520 1530 1540 1550
SDDPMLPSPD QLRRKVLLKN KKLKAHQTPV DILKQKAHQL ASMQAQAFTG
1560 1570 1580 1590 1600
GNANPPPASN EEEEDEEDEY DYDYESLSDD NILEDRPENK SCADKLQFEY
1610 1620 1630 1640 1650
NEEVPKRIKK ADNSSGNKGK VYDMELGEEF YLPQNKKESR QIAPELSDLV
1660 1670 1680 1690 1700
IYCQAVKFPG LSTLNSSGSS RGKERKSRKS IFGNNPGRMS PGETAPFNRT
1710 1720 1730 1740 1750
SGKGSCEGMR HTWEESSPLS PSTSLSAIIR TPKCYHISSL NENAAKRLCR
1760 1770 1780 1790 1800
RGSQKLIQHT AYQLLRTYPA ATRIDSSNPN PIMFWLHGIQ LVALNYQTDD
1810 1820 1830 1840 1850
LPLHLNAAMF EANGGCGYVL KPPVLWDKSC PMYQKFSPLE RDLDNLDPAI
1860 1870 1880 1890 1900
YSLTIISGQN VCPSNSTGSP CIEVDVLGMP LDSCHFRTKP IHRNTLNPMW
1910 1920 1930 1940 1950
NEQFLFRVHF EDLVFLRFAV VENNSSAITA QRIIPLRALK RGYRHLQLRN
1960 1970 1980 1990 2000
LHNEILEISS LFINSRRMEE NPSGSSMPAS LMFNTEERKC SQTHKVTVHG
2010 2020 2030 2040 2050
VPGPEPFAVF TINEGTKAKQ LLQQVLAVDQ DTKCTATDYF LMEEKHFISK
2060 2070 2080 2090 2100
EKNECRKQPF QRAVGPEEDI VQILNSWFPE EGYVGRIVLK PQQETLEEKS
2110 2120 2130 2140 2150
IVFDDKEVIL SSEEESFFVQ VHDVSPEQPR TVIKAPRVST AQDVIQQTLC
2160 2170 2180 2190 2200
KAKYSYSILN NPNPCDYVLL EEVLKDAANK KSSTPKSSQR ILLDQECVFQ
2210 2220 2230 2240 2250
AQSKWKGAGK FILKLKEQVQ ASREDKRRGI SFASELKKLT KSTKQSRGLP
2260 2270 2280
SPPQLVASES VQSKEEKPVG ALSSSDTVGY QQ
Length:2,282
Mass (Da):255,047
Last modified:July 27, 2011 - v3
Checksum:iAA288A76304E43ED
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R1Q8S4R1Q8_MOUSE
Phosphoinositide phospholipase C
Plce1
2,296Annotation score:
S4R168S4R168_MOUSE
1-phosphatidylinositol 4,5-bisphosp...
Plce1
74Annotation score:

Sequence cautioni

The sequence BAC29099 differs from that shown. Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti295N → S in BAC00906 (PubMed:12752375).Curated1
Sequence conflicti295N → S in AAI38350 (PubMed:15489334).Curated1
Sequence conflicti295N → S in AAI38351 (PubMed:15489334).Curated1
Sequence conflicti1798T → A in AAF40208 (PubMed:11146508).Curated1
Sequence conflicti1829S → N in AAF40208 (PubMed:11146508).Curated1
Sequence conflicti2215 – 2217LKE → VKD in BAC29099 (PubMed:16141072).Curated3
Sequence conflicti2233A → G in BAC29099 (PubMed:16141072).Curated1
Sequence conflicti2236L → V in BAC29099 (PubMed:16141072).Curated1
Sequence conflicti2239L → V in BAC29099 (PubMed:16141072).Curated1
Sequence conflicti2275S → T in BAC29099 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076247 mRNA Translation: BAC00906.1
AC111023 Genomic DNA No translation available.
AC158905 Genomic DNA No translation available.
BC138349 mRNA Translation: AAI38350.1
BC138350 mRNA Translation: AAI38351.1
AK122521 mRNA Translation: BAC65803.1
AK035546 mRNA Translation: BAC29099.1 Sequence problems.
AK162236 mRNA Translation: BAE36807.1
AF233885 mRNA Translation: AAF40208.1
CCDSiCCDS37973.1
RefSeqiNP_062534.2, NM_019588.2
XP_011245682.1, XM_011247380.1
UniGeneiMm.34031

Genome annotation databases

EnsembliENSMUST00000169713; ENSMUSP00000130604; ENSMUSG00000024998
ENSMUST00000182481; ENSMUSP00000138360; ENSMUSG00000024998
GeneIDi74055
KEGGimmu:74055
UCSCiuc008hjp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076247 mRNA Translation: BAC00906.1
AC111023 Genomic DNA No translation available.
AC158905 Genomic DNA No translation available.
BC138349 mRNA Translation: AAI38350.1
BC138350 mRNA Translation: AAI38351.1
AK122521 mRNA Translation: BAC65803.1
AK035546 mRNA Translation: BAC29099.1 Sequence problems.
AK162236 mRNA Translation: BAE36807.1
AF233885 mRNA Translation: AAF40208.1
CCDSiCCDS37973.1
RefSeqiNP_062534.2, NM_019588.2
XP_011245682.1, XM_011247380.1
UniGeneiMm.34031

3D structure databases

ProteinModelPortaliQ8K4S1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60738N
IntActiQ8K4S1, 5 interactors
MINTiQ8K4S1
STRINGi10090.ENSMUSP00000130604

PTM databases

iPTMnetiQ8K4S1
PhosphoSitePlusiQ8K4S1

Proteomic databases

MaxQBiQ8K4S1
PaxDbiQ8K4S1
PRIDEiQ8K4S1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000169713; ENSMUSP00000130604; ENSMUSG00000024998
ENSMUST00000182481; ENSMUSP00000138360; ENSMUSG00000024998
GeneIDi74055
KEGGimmu:74055
UCSCiuc008hjp.1 mouse

Organism-specific databases

CTDi51196
MGIiMGI:1921305 Plce1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00730000110782
HOVERGENiHBG059220
InParanoidiQ8K4S1
KOiK05860
TreeFamiTF314432

Enzyme and pathway databases

BRENDAi3.1.4.11 3474
ReactomeiR-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol

Miscellaneous databases

PROiPR:Q8K4S1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024998 Expressed in 261 organ(s), highest expression level in stria vascularis of cochlear duct
CleanExiMM_PLCE1
ExpressionAtlasiQ8K4S1 baseline and differential
GenevisibleiQ8K4S1 MM

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR028398 PLC-epsilon1
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
IPR000159 RA_dom
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF6 PTHR10336:SF6, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF00788 RA, 1 hit
PF00617 RasGEF, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SM00147 RasGEF, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF48366 SSF48366, 1 hit
SSF51695 SSF51695, 1 hit
SSF54236 SSF54236, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
PS50200 RA, 1 hit
PS50009 RASGEF_CAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCE1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4S1
Secondary accession number(s): B9EHS1
, E9Q5G0, Q3TS68, Q80TC4, Q8BZF3, Q9JKM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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