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Protein

Endonuclease 8-like 1

Gene

Neil1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.2 Publications

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNACurated1
Active sitei3Proton donorCurated1
Active sitei54Proton donor; for beta-elimination activityCurated1
Binding sitei176DNABy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-110329 Cleavage of the damaged pyrimidine
R-MMU-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 1 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil1
DNA-(apurinic or apyrimidinic site) lyase Neil1
Endonuclease VIII-like 1
Nei homolog 1
Short name:
NEH1
Nei-like protein 1
Gene namesi
Name:Neil1
Synonyms:Nei1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1920024 Neil1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001709062 – 389Endonuclease 8-like 1Add BLAST388

Proteomic databases

EPDiQ8K4Q6
PaxDbiQ8K4Q6
PeptideAtlasiQ8K4Q6
PRIDEiQ8K4Q6

PTM databases

PhosphoSitePlusiQ8K4Q6

Expressioni

Tissue specificityi

Detected in heart, spleen and lung.1 Publication

Inductioni

Up-regulated during S-phase.By similarity

Gene expression databases

BgeeiENSMUSG00000032298 Expressed in 234 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_NEIL1
GenevisibleiQ8K4Q6 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034842

Structurei

3D structure databases

ProteinModelPortaliQ8K4Q6
SMRiQ8K4Q6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IEAW Eukaryota
ENOG410ZTEH LUCA
GeneTreeiENSGT00390000016671
HOGENOMiHOG000067872
HOVERGENiHBG052592
InParanoidiQ8K4Q6
KOiK10567
OMAiHLASHFV
OrthoDBiEOG091G07LM
PhylomeDBiQ8K4Q6
TreeFamiTF333272

Family and domain databases

Gene3Di3.20.190.10, 1 hit
InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR015371 Endonuclease-VIII_DNA-bd
IPR012319 FPG_cat
IPR035937 MutM-like_N-ter
IPR010979 Ribosomal_S13-like_H2TH
PfamiView protein in Pfam
PF01149 Fapy_DNA_glyco, 1 hit
PF06831 H2TH, 1 hit
PF09292 Neil1-DNA_bind, 1 hit
SMARTiView protein in SMART
SM00898 Fapy_DNA_glyco, 1 hit
SM01232 H2TH, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF81624 SSF81624, 1 hit
PROSITEiView protein in PROSITE
PS51068 FPG_CAT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8K4Q6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPEGPELHLA SHFVNETCKG LVFGGCVEKS SVSRNPEVPF ESSAYHISAL
60 70 80 90 100
ARGKELRLTL SPLPGSQPPQ KPLSLVFRFG MSGSFQLVPA EALPRHAHLR
110 120 130 140 150
FYTAPPAPRL ALCFVDIRRF GHWDPGGEWQ PGRGPCVLLE YERFRENVLR
160 170 180 190 200
NLSDKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARTVLEA
210 220 230 240 250
LQQCRPSPEL TLSQKIKAKL QNPDLLELCH LVPKEVVQLG GKGYGPERGE
260 270 280 290 300
EDFAAFRAWL RCYGVPGMSS LRDRHGRTIW FQGDPGPLAP KGGRSQKKKS
310 320 330 340 350
QETQLGAEDR KEDLPLSSKS VSRMRRARKH PPKRIAQQSE GAGLQQNQET
360 370 380
PTAPEKGKRR GQRASTGHRR RPKTIPDTRP REAGESSAS
Length:389
Mass (Da):43,586
Last modified:January 23, 2007 - v3
Checksum:iE3F1A21DDE2CB5AD
GO
Isoform 2 (identifier: Q8K4Q6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-389: GKGYGPERGE...PREAGESSAS → EAWGGQDGRRPLP

Note: No experimental confirmation available.
Show »
Length:253
Mass (Da):28,453
Checksum:i8BE7DE61651A166A
GO

Sequence cautioni

The sequence BAB28790 differs from that shown. Reason: Frameshift at position 344.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012207241 – 389GKGYG…ESSAS → EAWGGQDGRRPLP in isoform 2. 1 PublicationAdd BLAST149

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB079069 mRNA Translation: BAC06477.1
AK013322 mRNA Translation: BAB28790.1 Frameshift.
BC043297 mRNA Translation: AAH43297.1
CCDSiCCDS23217.1 [Q8K4Q6-1]
RefSeqiNP_082623.1, NM_028347.2 [Q8K4Q6-1]
XP_006511548.1, XM_006511485.3 [Q8K4Q6-1]
XP_006511549.1, XM_006511486.2 [Q8K4Q6-1]
XP_011241120.1, XM_011242818.2
UniGeneiMm.35749
Mm.488747

Genome annotation databases

EnsembliENSMUST00000034842; ENSMUSP00000034842; ENSMUSG00000032298 [Q8K4Q6-1]
ENSMUST00000186410; ENSMUSP00000141048; ENSMUSG00000032298 [Q8K4Q6-1]
ENSMUST00000190245; ENSMUSP00000139917; ENSMUSG00000032298 [Q8K4Q6-1]
GeneIDi72774
KEGGimmu:72774
UCSCiuc009puh.1 mouse [Q8K4Q6-1]
uc009puk.1 mouse [Q8K4Q6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNEIL1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Q6
Secondary accession number(s): Q80V58, Q9CYT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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