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Protein

E3 ubiquitin-protein ligase HECW1

Gene

Hecw1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1572Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-4641258 Degradation of DVL
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HECW1 (EC:2.3.2.26)
Alternative name(s):
HECT, C2 and WW domain-containing protein 1
HECT-type E3 ubiquitin transferase HECW1
NEDD4-like E3 ubiquitin-protein ligase 1
Short name:
mNEDL1
Gene namesi
Name:Hecw1
Synonyms:Kiaa0322, Nedl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2444115 Hecw1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776661 – 1604E3 ubiquitin-protein ligase HECW1Add BLAST1604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei871PhosphoserineCombined sources1
Modified residuei935PhosphoserineCombined sources1
Modified residuei937PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K4P8
PeptideAtlasiQ8K4P8
PRIDEiQ8K4P8

PTM databases

iPTMnetiQ8K4P8
PhosphoSitePlusiQ8K4P8

Expressioni

Tissue specificityi

Predominantly expressed in neurons of the spinal cord.

Gene expression databases

BgeeiENSMUSG00000021301
ExpressionAtlasiQ8K4P8 baseline and differential
GenevisibleiQ8K4P8 MM

Interactioni

Subunit structurei

Interacts with DVL1 and SSR3.By similarity

Protein-protein interaction databases

BioGridi220495, 1 interactor
IntActiQ8K4P8, 1 interactor
STRINGi10090.ENSMUSP00000106145

Structurei

3D structure databases

ProteinModelPortaliQ8K4P8
SMRiQ8K4P8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 302C2PROSITE-ProRule annotationAdd BLAST111
Domaini826 – 859WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini1016 – 1049WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini1269 – 1604HECTPROSITE-ProRule annotationAdd BLAST336

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili871 – 898Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi407 – 524Glu-richAdd BLAST118
Compositional biasi671 – 703Cys-richAdd BLAST33

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0940 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00760000118966
HOVERGENiHBG057414
InParanoidiQ8K4P8
KOiK12167
OMAiTHYIRIH
OrthoDBiEOG091G0A54
TreeFamiTF313938

Family and domain databases

CDDicd08691 C2_NEDL1-like, 1 hit
cd00078 HECTc, 1 hit
cd00201 WW, 2 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037795 C2_HECW
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR032348 HECW_N
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00632 HECT, 1 hit
PF16562 HECW_N, 1 hit
PF00397 WW, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00119 HECTc, 1 hit
SM00456 WW, 2 hits
SUPFAMiSSF51045 SSF51045, 2 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50237 HECT, 1 hit
PS01159 WW_DOMAIN_1, 2 hits
PS50020 WW_DOMAIN_2, 2 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Named isoforms=2.
Isoform 1 (identifier: Q8K4P8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN
60 70 80 90 100
IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW
110 120 130 140 150
DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY
160 170 180 190 200
FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG
210 220 230 240 250
SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE
260 270 280 290 300
RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK
310 320 330 340 350
LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHADD
360 370 380 390 400
EEISLSAEPE SSAETQDSIM NSMVGNSNGE PSGDATEFCK DAKPESPSEG
410 420 430 440 450
NGVNSSENQN QEHAGPVEEA AGAMEARDGS NVSEAPEEPG ELQDPEQHDT
460 470 480 490 500
QPTLSAEEVA EGLPLDEDSP SSLLPEENTA LGSKVEEETV PENGAREEEM
510 520 530 540 550
QKGKDEEEEE EDVSTLEQGE PGLELRVSVR KKSRPCSLPV SELETVIASA
560 570 580 590 600
CGDAETPRTH YIRIHTLLHS MPSAQRGSTT EEEDGLEEES TLKESSEKDG
610 620 630 640 650
LSEVDTIAAD PQSMEDGESD GATLCMAPSD CSGGHFSSLS KGIGAGQDGE
660 670 680 690 700
AHPSTGSESD SSPQQGADHS CEGCDASCCS PSCYSTSCYS SSCYSSSCYS
710 720 730 740 750
SSCYNGNNRF ASHTRFSSVD SAKISESTVF SSQEDEEEEN SAFESVPDSV
760 770 780 790 800
QSPELDPEST NGAGPWQDEL AAPGGNAARS TEGLESPMAG PSNRREGECP
810 820 830 840 850
ILHNSQPISQ LPSLRPEHHH YPTIDEPLPP NWEARIDSHG RVFYVDHINR
860 870 880 890 900
TTTWQRPSMA PTPDGMIRSG SVHQMEQLNR RYQNIQRTMA TERAEEDSGN
910 920 930 940 950
QNSEQIPDGG GGGGGGSDSE AESSQSSLDL RREGSLSPVN SQKVTLLLQS
960 970 980 990 1000
PAVKFITNPE FFTVLHANYS AYRVFTSSTC LKHMILKVRR DARNFERYQH
1010 1020 1030 1040 1050
NRDLVNFINM FADTRLELPR GWEIKTDHQG KSFFVDHNSR ATTFIDPRIP
1060 1070 1080 1090 1100
LQNGRLPNHL THRQHLQRLR SYSAGEASEV SRNRGASLLA RPGHSLIAAI
1110 1120 1130 1140 1150
RSQHQHESLP LAYNDKIVAF LRQPNIFEML QERQPSLARN HTLREKIHYI
1160 1170 1180 1190 1200
RTEGNHGLDK LSCDADLVIL LSLFEEEIMS YVPLQSAFHP GYSFSPRCSP
1210 1220 1230 1240 1250
CSSPQNSPGL QRASARAPSP YRRDFEAKLR NFYRKLEAKG FGQGPGKIKL
1260 1270 1280 1290 1300
IIRRDHLLEG TFNQVMAYSR KELQRNKLYI TFVGEEGLDY SGPSREFFFL
1310 1320 1330 1340 1350
LSQELFNPYY GLFEYSANDT YTVQISPMSA FVENYLEWFR FSGRILGLAL
1360 1370 1380 1390 1400
IHQYLLDAFF TRPFYKGLLK LPCDLSDLEY LDEEFHQSLQ WMKDNNITDI
1410 1420 1430 1440 1450
LDLTFTVNEE VFGQVTEREL KSGGANTQVT EKNKKEYIER MVKWRVERGV
1460 1470 1480 1490 1500
VQQTEALLRG FYEVVDSRLV SVFDARELEL VIAGTAEIDL NDWRNNTEYR
1510 1520 1530 1540 1550
GGYHDGHLVI RWFWAAVERF NNEQRLRLLQ FVTGTSSVPY EGFAALRGSN
1560 1570 1580 1590 1600
GLRRFCIEKW GKITSLPRAH TCFNRLDLPP YPSYSMLYEK LLTAVEETST

FGLE
Length:1,604
Mass (Da):179,467
Last modified:July 27, 2011 - v3
Checksum:i0643EE0129A08E0D
GO
Isoform 2 (identifier: Q8K4P8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     782-795: Missing.

Note: No experimental confirmation available.
Show »
Length:1,590
Mass (Da):177,984
Checksum:i51C7D0DA9432CED2
GO
Isoform 3 (identifier: Q8K4P8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MLLHLCSV → MICAQLEAA
     316-1604: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):35,504
Checksum:iE3336039B30263B4
GO
Isoform 4 (identifier: Q8K4P8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-1604: Missing.

Note: No experimental confirmation available.
Show »
Length:315
Mass (Da):35,470
Checksum:i2D5E366B748ED3E2
GO

Sequence cautioni

The sequence BAC28516 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167S → R in BAB97389 (PubMed:14684739).Curated1
Sequence conflicti823T → A in BAB97389 (PubMed:14684739).Curated1
Sequence conflicti1036D → G in BAB97389 (PubMed:14684739).Curated1
Sequence conflicti1248I → V in BAB97389 (PubMed:14684739).Curated1
Sequence conflicti1356L → P in BAB97389 (PubMed:14684739).Curated1
Sequence conflicti1538V → M in BAB97389 (PubMed:14684739).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0230741 – 8MLLHLCSV → MICAQLEAA in isoform 3. 1 Publication8
Alternative sequenceiVSP_023075316 – 1604Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST1289
Alternative sequenceiVSP_023076782 – 795Missing in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033922 mRNA Translation: BAC28516.1 Different initiation.
AK047678 mRNA Translation: BAC33122.1
AK051569 mRNA Translation: BAC34677.1
AK053694 mRNA Translation: BAC35477.1
AC092710 Genomic DNA No translation available.
AC154511 Genomic DNA No translation available.
AC154615 Genomic DNA No translation available.
AC161249 Genomic DNA No translation available.
CT025604 Genomic DNA No translation available.
AB083710 mRNA Translation: BAB97389.1
AK172932 mRNA Translation: BAD32210.1
CCDSiCCDS49207.1 [Q8K4P8-1]
RefSeqiNP_001074817.3, NM_001081348.3 [Q8K4P8-1]
XP_006516873.1, XM_006516810.3 [Q8K4P8-1]
XP_006516874.1, XM_006516811.1 [Q8K4P8-1]
XP_006516875.1, XM_006516812.3 [Q8K4P8-1]
UniGeneiMm.125298

Genome annotation databases

EnsembliENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301 [Q8K4P8-1]
GeneIDi94253
KEGGimmu:94253
UCSCiuc007pnd.2 mouse [Q8K4P8-1]
uc007png.2 mouse [Q8K4P8-4]
uc007pnh.2 mouse [Q8K4P8-3]
uc011ywk.1 mouse [Q8K4P8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHECW1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4P8
Secondary accession number(s): D3YZ67
, Q6A086, Q8BIA6, Q8BKC2, Q8BKL3, Q8BKZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 121 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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