UniProtKB - Q8K4P8 (HECW1_MOUSE)

BLAST

>sp|Q8K4P8|HECW1_MOUSE E3 ubiquitin-protein ligase HECW1 OS=Mus musculus OX=10090 GN=Hecw1 PE=1 SV=3
MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDA
ITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEV
SSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVK
NSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSI
FPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGK
LSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPE
SSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEA
AGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTA
LGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPV
SELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDG
LSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESD
SSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVD
SAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARS
TEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHG
RVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGN
QNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPE
FFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPR
GWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEV
SRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARN
HTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSP
CSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEG
TFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDT
YTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEY
LDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIER
MVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYR
GGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKW
GKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

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History
Entry version 124 (05 Dec 2018)
Sequence version 3 (27 Jul 2011)
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Protein

E3 ubiquitin-protein ligase HECW1

Gene

Hecw1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1.By similarity1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	1572	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ubiquitin protein ligase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of sodium ion transmembrane transporter activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
positive regulation of protein catabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
proteasome-mediated ubiquitin-dependent protein catabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein polyubiquitination
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein ubiquitination
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of dendrite morphogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ubiquitin-dependent protein catabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway

Enzyme and pathway databases

Reactomeⁱ	R-MMU-4641258 Degradation of DVL
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 ubiquitin-protein ligase HECW1 (EC:2.3.2.26) Alternative name(s): HECT, C2 and WW domain-containing protein 1 HECT-type E3 ubiquitin transferase HECW1 NEDD4-like E3 ubiquitin-protein ligase 1 Short name: mNEDL1
Gene namesⁱ	Name:Hecw1 Synonyms:Kiaa0322, Nedl1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 13

Organism-specific databases

MGIⁱ	MGI:2444115 Hecw1

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000277666	1 – 1604	E3 ubiquitin-protein ligase HECW1Add BLAST		1604

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	871	PhosphoserineCombined sources	1
Modified residueⁱ	935	PhosphoserineCombined sources	1
Modified residueⁱ	937	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q8K4P8
PeptideAtlas More...PeptideAtlasⁱ	Q8K4P8
PRIDEⁱ	Q8K4P8

PTM databases

iPTMnetⁱ	Q8K4P8
PhosphoSitePlusⁱ	Q8K4P8

Expressionⁱ

Tissue specificityⁱ

Predominantly expressed in neurons of the spinal cord.

Gene expression databases

Bgeeⁱ	ENSMUSG00000021301 Expressed in 112 organ(s), highest expression level in retina
ExpressionAtlasⁱ	Q8K4P8 baseline and differential
Genevisibleⁱ	Q8K4P8 MM

Interactionⁱ

Subunit structureⁱ

Interacts with DVL1 and SSR3.By similarity

Protein-protein interaction databases

BioGridⁱ	220495, 2 interactors
IntActⁱ	Q8K4P8, 1 interactor
STRINGⁱ	10090.ENSMUSP00000106145

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8K4P8
SMRⁱ	Q8K4P8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	192 – 302	C2PROSITE-ProRule annotationAdd BLAST	111
Domainⁱ	826 – 859	WW 1PROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	1016 – 1049	WW 2PROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	1269 – 1604	HECTPROSITE-ProRule annotationAdd BLAST	336

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	871 – 898	Sequence analysisAdd BLAST		28

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	407 – 524	Glu-richAdd BLAST		118
Compositional biasⁱ	671 – 703	Cys-richAdd BLAST		33

Keywords - Domainⁱ

Coiled coil, Repeat

Phylogenomic databases

eggNOGⁱ	KOG0940 Eukaryota COG5021 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000158294
HOVERGENⁱ	HBG057414
InParanoidⁱ	Q8K4P8
KEGG Orthology (KO) More...KOⁱ	K12167
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0A54
TreeFamⁱ	TF313938

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd08691 C2_NEDL1-like, 1 hit cd00078 HECTc, 1 hit cd00201 WW, 2 hits
Gene3Dⁱ	2.60.40.150, 1 hit
InterProⁱ	View protein in InterPro IPR000008 C2_dom IPR035892 C2_domain_sf IPR037795 C2_HECW IPR000569 HECT_dom IPR035983 Hect_E3_ubiquitin_ligase IPR032348 HECW_N IPR001202 WW_dom IPR036020 WW_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00168 C2, 1 hit PF00632 HECT, 1 hit PF16562 HECW_N, 1 hit PF00397 WW, 1 hit
SMARTⁱ	View protein in SMART SM00239 C2, 1 hit SM00119 HECTc, 1 hit SM00456 WW, 2 hits
SUPFAMⁱ	SSF51045 SSF51045, 2 hits SSF56204 SSF56204, 1 hit
PROSITEⁱ	View protein in PROSITE PS50004 C2, 1 hit PS50237 HECT, 1 hit PS01159 WW_DOMAIN_1, 2 hits PS50020 WW_DOMAIN_2, 2 hits

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: Named isoforms=2.

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q8K4P8-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN 
        60         70         80         90        100
IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW 
       110        120        130        140        150
DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY 
       160        170        180        190        200
FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG 
       210        220        230        240        250
SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE 
       260        270        280        290        300
RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK 
       310        320        330        340        350
LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHADD 
       360        370        380        390        400
EEISLSAEPE SSAETQDSIM NSMVGNSNGE PSGDATEFCK DAKPESPSEG 
       410        420        430        440        450
NGVNSSENQN QEHAGPVEEA AGAMEARDGS NVSEAPEEPG ELQDPEQHDT 
       460        470        480        490        500
QPTLSAEEVA EGLPLDEDSP SSLLPEENTA LGSKVEEETV PENGAREEEM 
       510        520        530        540        550
QKGKDEEEEE EDVSTLEQGE PGLELRVSVR KKSRPCSLPV SELETVIASA 
       560        570        580        590        600
CGDAETPRTH YIRIHTLLHS MPSAQRGSTT EEEDGLEEES TLKESSEKDG 
       610        620        630        640        650
LSEVDTIAAD PQSMEDGESD GATLCMAPSD CSGGHFSSLS KGIGAGQDGE 
       660        670        680        690        700
AHPSTGSESD SSPQQGADHS CEGCDASCCS PSCYSTSCYS SSCYSSSCYS 
       710        720        730        740        750
SSCYNGNNRF ASHTRFSSVD SAKISESTVF SSQEDEEEEN SAFESVPDSV 
       760        770        780        790        800
QSPELDPEST NGAGPWQDEL AAPGGNAARS TEGLESPMAG PSNRREGECP 
       810        820        830        840        850
ILHNSQPISQ LPSLRPEHHH YPTIDEPLPP NWEARIDSHG RVFYVDHINR 
       860        870        880        890        900
TTTWQRPSMA PTPDGMIRSG SVHQMEQLNR RYQNIQRTMA TERAEEDSGN 
       910        920        930        940        950
QNSEQIPDGG GGGGGGSDSE AESSQSSLDL RREGSLSPVN SQKVTLLLQS 
       960        970        980        990       1000
PAVKFITNPE FFTVLHANYS AYRVFTSSTC LKHMILKVRR DARNFERYQH 
      1010       1020       1030       1040       1050
NRDLVNFINM FADTRLELPR GWEIKTDHQG KSFFVDHNSR ATTFIDPRIP 
      1060       1070       1080       1090       1100
LQNGRLPNHL THRQHLQRLR SYSAGEASEV SRNRGASLLA RPGHSLIAAI 
      1110       1120       1130       1140       1150
RSQHQHESLP LAYNDKIVAF LRQPNIFEML QERQPSLARN HTLREKIHYI 
      1160       1170       1180       1190       1200
RTEGNHGLDK LSCDADLVIL LSLFEEEIMS YVPLQSAFHP GYSFSPRCSP 
      1210       1220       1230       1240       1250
CSSPQNSPGL QRASARAPSP YRRDFEAKLR NFYRKLEAKG FGQGPGKIKL 
      1260       1270       1280       1290       1300
IIRRDHLLEG TFNQVMAYSR KELQRNKLYI TFVGEEGLDY SGPSREFFFL 
      1310       1320       1330       1340       1350
LSQELFNPYY GLFEYSANDT YTVQISPMSA FVENYLEWFR FSGRILGLAL 
      1360       1370       1380       1390       1400
IHQYLLDAFF TRPFYKGLLK LPCDLSDLEY LDEEFHQSLQ WMKDNNITDI 
      1410       1420       1430       1440       1450
LDLTFTVNEE VFGQVTEREL KSGGANTQVT EKNKKEYIER MVKWRVERGV 
      1460       1470       1480       1490       1500
VQQTEALLRG FYEVVDSRLV SVFDARELEL VIAGTAEIDL NDWRNNTEYR 
      1510       1520       1530       1540       1550
GGYHDGHLVI RWFWAAVERF NNEQRLRLLQ FVTGTSSVPY EGFAALRGSN 
      1560       1570       1580       1590       1600
GLRRFCIEKW GKITSLPRAH TCFNRLDLPP YPSYSMLYEK LLTAVEETST 

FGLE

Length:1,604

Mass (Da):179,467

Last modified:July 27, 2011 - v3

Checksum:ⁱ0643EE0129A08E0D

Isoform 2 (identifier: Q8K4P8-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
782-795: Missing.

« Hide

        10         20         30         40         50
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN 
        60         70         80         90        100
IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW 
       110        120        130        140        150
DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY 
       160        170        180        190        200
FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG 
       210        220        230        240        250
SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE 
       260        270        280        290        300
RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK 
       310        320        330        340        350
LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHADD 
       360        370        380        390        400
EEISLSAEPE SSAETQDSIM NSMVGNSNGE PSGDATEFCK DAKPESPSEG 
       410        420        430        440        450
NGVNSSENQN QEHAGPVEEA AGAMEARDGS NVSEAPEEPG ELQDPEQHDT 
       460        470        480        490        500
QPTLSAEEVA EGLPLDEDSP SSLLPEENTA LGSKVEEETV PENGAREEEM 
       510        520        530        540        550
QKGKDEEEEE EDVSTLEQGE PGLELRVSVR KKSRPCSLPV SELETVIASA 
       560        570        580        590        600
CGDAETPRTH YIRIHTLLHS MPSAQRGSTT EEEDGLEEES TLKESSEKDG 
       610        620        630        640        650
LSEVDTIAAD PQSMEDGESD GATLCMAPSD CSGGHFSSLS KGIGAGQDGE 
       660        670        680        690        700
AHPSTGSESD SSPQQGADHS CEGCDASCCS PSCYSTSCYS SSCYSSSCYS 
       710        720        730        740        750
SSCYNGNNRF ASHTRFSSVD SAKISESTVF SSQEDEEEEN SAFESVPDSV 
       760        770        780        790        800
QSPELDPEST NGAGPWQDEL AAPGGNAARS TEGECPILHN SQPISQLPSL 
       810        820        830        840        850
RPEHHHYPTI DEPLPPNWEA RIDSHGRVFY VDHINRTTTW QRPSMAPTPD 
       860        870        880        890        900
GMIRSGSVHQ MEQLNRRYQN IQRTMATERA EEDSGNQNSE QIPDGGGGGG 
       910        920        930        940        950
GGSDSEAESS QSSLDLRREG SLSPVNSQKV TLLLQSPAVK FITNPEFFTV 
       960        970        980        990       1000
LHANYSAYRV FTSSTCLKHM ILKVRRDARN FERYQHNRDL VNFINMFADT 
      1010       1020       1030       1040       1050
RLELPRGWEI KTDHQGKSFF VDHNSRATTF IDPRIPLQNG RLPNHLTHRQ 
      1060       1070       1080       1090       1100
HLQRLRSYSA GEASEVSRNR GASLLARPGH SLIAAIRSQH QHESLPLAYN 
      1110       1120       1130       1140       1150
DKIVAFLRQP NIFEMLQERQ PSLARNHTLR EKIHYIRTEG NHGLDKLSCD 
      1160       1170       1180       1190       1200
ADLVILLSLF EEEIMSYVPL QSAFHPGYSF SPRCSPCSSP QNSPGLQRAS 
      1210       1220       1230       1240       1250
ARAPSPYRRD FEAKLRNFYR KLEAKGFGQG PGKIKLIIRR DHLLEGTFNQ 
      1260       1270       1280       1290       1300
VMAYSRKELQ RNKLYITFVG EEGLDYSGPS REFFFLLSQE LFNPYYGLFE 
      1310       1320       1330       1340       1350
YSANDTYTVQ ISPMSAFVEN YLEWFRFSGR ILGLALIHQY LLDAFFTRPF 
      1360       1370       1380       1390       1400
YKGLLKLPCD LSDLEYLDEE FHQSLQWMKD NNITDILDLT FTVNEEVFGQ 
      1410       1420       1430       1440       1450
VTERELKSGG ANTQVTEKNK KEYIERMVKW RVERGVVQQT EALLRGFYEV 
      1460       1470       1480       1490       1500
VDSRLVSVFD ARELELVIAG TAEIDLNDWR NNTEYRGGYH DGHLVIRWFW 
      1510       1520       1530       1540       1550
AAVERFNNEQ RLRLLQFVTG TSSVPYEGFA ALRGSNGLRR FCIEKWGKIT 
      1560       1570       1580       1590
SLPRAHTCFN RLDLPPYPSY SMLYEKLLTA VEETSTFGLE

Note: No experimental confirmation available.

Show »

Length:1,590

Mass (Da):177,984

Checksum:ⁱ51C7D0DA9432CED2

Isoform 3 (identifier: Q8K4P8-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-8: MLLHLCSV → MICAQLEAA
316-1604: Missing.

« Hide

        10         20         30         40         50
MICAQLEAAK NLYQNRFLGL AAMASPSRNS QSRRRCKEPL RYSYNPDQFH 
        60         70         80         90        100
NIDIRNGAHD AITIPRSTSD TDLVTSDSRS TLMVSSSYYS IGHSQDLVIH 
       110        120        130        140        150
WDIKEEVDAG DWIGMYLIGE VSSENFLDYK NRGVNGSHRG QIIWKIDASS 
       160        170        180        190        200
YFVESETKIC FKYYHGVSGA LRATTPSVTV KNSAAPIFKG IGSEETAQSQ 
       210        220        230        240        250
GSRRLISFSL SDFQAMGLKK GMFFNPDPYL KISIQPGKHS IFPALPHHGQ 
       260        270        280        290        300
ERRSTIIGNT VNPIWQAEHF SFVSLPTDVL EIEVKDKFAK SRPIIKRFLG 
       310 
KLSMPVQRLL ERHAIG

Note: No experimental confirmation available.

Show »

Length:316

Mass (Da):35,504

Checksum:ⁱE3336039B30263B4

Isoform 4 (identifier: Q8K4P8-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
316-1604: Missing.

« Hide

        10         20         30         40         50
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN 
        60         70         80         90        100
IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW 
       110        120        130        140        150
DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY 
       160        170        180        190        200
FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG 
       210        220        230        240        250
SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE 
       260        270        280        290        300
RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK 
       310 
LSMPVQRLLE RHAIG

Note: No experimental confirmation available.

Show »

Length:315

Mass (Da):35,470

Checksum:ⁱ2D5E366B748ED3E2

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A1Y7VJ05	A0A1Y7VJ05_MOUSE	E3 ubiquitin-protein ligase HECW1 E3 ubiquitin-protein ligase HECW1	Hecw1	1,177	Annotation score:
A0A1Y7VJB7	A0A1Y7VJB7_MOUSE	E3 ubiquitin-protein ligase HECW1 E3 ubiquitin-protein ligase HECW1	Hecw1	74	Annotation score:
A0A1Y7VJ41	A0A1Y7VJ41_MOUSE	E3 ubiquitin-protein ligase HECW1 E3 ubiquitin-protein ligase HECW1	Hecw1	132	Annotation score:
A0A1Y7VMF2	A0A1Y7VMF2_MOUSE	E3 ubiquitin-protein ligase HECW1 E3 ubiquitin-protein ligase HECW1	Hecw1	23	Annotation score:

Sequence cautionⁱ

The sequence BAC28516 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	167	S → R in BAB97389 (PubMed:14684739).Curated	1
Sequence conflictⁱ	823	T → A in BAB97389 (PubMed:14684739).Curated	1
Sequence conflictⁱ	1036	D → G in BAB97389 (PubMed:14684739).Curated	1
Sequence conflictⁱ	1248	I → V in BAB97389 (PubMed:14684739).Curated	1
Sequence conflictⁱ	1356	L → P in BAB97389 (PubMed:14684739).Curated	1
Sequence conflictⁱ	1538	V → M in BAB97389 (PubMed:14684739).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_023074	1 – 8	MLLHLCSV → MICAQLEAA in isoform 3. 1 Publication	8
Alternative sequenceⁱVSP_023075	316 – 1604	Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST	1289
Alternative sequenceⁱVSP_023076	782 – 795	Missing in isoform 2. 1 PublicationAdd BLAST	14

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK033922 mRNA Translation: BAC28516.1 Different initiation. AK047678 mRNA Translation: BAC33122.1 AK051569 mRNA Translation: BAC34677.1 AK053694 mRNA Translation: BAC35477.1 AC092710 Genomic DNA No translation available. AC154511 Genomic DNA No translation available. AC154615 Genomic DNA No translation available. AC161249 Genomic DNA No translation available. CT025604 Genomic DNA No translation available. AB083710 mRNA Translation: BAB97389.1 AK172932 mRNA Translation: BAD32210.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS49207.1 [Q8K4P8-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001074817.3, NM_001081348.3 [Q8K4P8-1] XP_006516873.1, XM_006516810.3 [Q8K4P8-1] XP_006516874.1, XM_006516811.1 [Q8K4P8-1] XP_006516875.1, XM_006516812.3 [Q8K4P8-1]
UniGeneⁱ	Mm.125298

Genome annotation databases

Ensemblⁱ	ENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301 [Q8K4P8-1]
GeneIDⁱ	94253
KEGGⁱ	mmu:94253
UCSC genome browser More...UCSCⁱ	uc007pnd.2 mouse [Q8K4P8-1] uc007png.2 mouse [Q8K4P8-4] uc007pnh.2 mouse [Q8K4P8-3] uc011ywk.1 mouse [Q8K4P8-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Protein	Similar proteins		Species	Score	Length	Source
Q8K4P8-3	E3 ubiquitin-protein ligase HECW1 (Fragment)		MOUSE		132	UniRef100_Q8K4P8-3
E3 ubiquitin-protein ligase HECW1 (Fragment)		MOUSE		74
UPI00001E8455		MOUSE		1605
UPI00001936C6		MOUSE		1583

Protein	Similar proteins		Species	Score	Length	Source
Q8K4P8	Isoform 4 of E3 ubiquitin-protein ligase HECW1		MOUSE		315	UniRef90_Q8K4P8
HECT, C2 and WW domain-containing E3 ubiquitin protein ligase 1		RAT		1594
UPI000A30A81A		MUSPA		1603
UPI000EAB0C16		MICOH		1597
UPI000B4EB281		MERUN		1339
+14

Protein	Similar proteins		Species	Score	Length	Source
Q8K4P8	E3 ubiquitin-protein ligase HECW2	)	HUMAN		1572	UniRef50_Q9P2P5
E3 ubiquitin-protein ligase HECW2		MOUSE		1578
E3 ubiquitin-protein ligase HECW2 isoform X1		DIPOR		1579
HECT, C2 and WW domain-containing E3 ubiquitin protein ligase 2		RAT		1578
E3 ubiquitin-protein ligase HECW2 isoform X1		MESAU		1577
+708

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK033922 mRNA Translation: BAC28516.1 Different initiation. AK047678 mRNA Translation: BAC33122.1 AK051569 mRNA Translation: BAC34677.1 AK053694 mRNA Translation: BAC35477.1 AC092710 Genomic DNA No translation available. AC154511 Genomic DNA No translation available. AC154615 Genomic DNA No translation available. AC161249 Genomic DNA No translation available. CT025604 Genomic DNA No translation available. AB083710 mRNA Translation: BAB97389.1 AK172932 mRNA Translation: BAD32210.1
CCDSⁱ	CCDS49207.1 [Q8K4P8-1]
RefSeqⁱ	NP_001074817.3, NM_001081348.3 [Q8K4P8-1] XP_006516873.1, XM_006516810.3 [Q8K4P8-1] XP_006516874.1, XM_006516811.1 [Q8K4P8-1] XP_006516875.1, XM_006516812.3 [Q8K4P8-1]
UniGeneⁱ	Mm.125298

3D structure databases

ProteinModelPortalⁱ	Q8K4P8
SMRⁱ	Q8K4P8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	220495, 2 interactors
IntActⁱ	Q8K4P8, 1 interactor
STRINGⁱ	10090.ENSMUSP00000106145

PTM databases

iPTMnetⁱ	Q8K4P8
PhosphoSitePlusⁱ	Q8K4P8

Proteomic databases

PaxDbⁱ	Q8K4P8
PeptideAtlasⁱ	Q8K4P8
PRIDEⁱ	Q8K4P8

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	94253
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301 [Q8K4P8-1]
GeneIDⁱ	94253
KEGGⁱ	mmu:94253
UCSCⁱ	uc007pnd.2 mouse [Q8K4P8-1] uc007png.2 mouse [Q8K4P8-4] uc007pnh.2 mouse [Q8K4P8-3] uc011ywk.1 mouse [Q8K4P8-2]

Organism-specific databases

CTDⁱ	23072
MGIⁱ	MGI:2444115 Hecw1
Rougeⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0940 Eukaryota COG5021 LUCA
GeneTreeⁱ	ENSGT00940000158294
HOVERGENⁱ	HBG057414
InParanoidⁱ	Q8K4P8
KOⁱ	K12167
OrthoDBⁱ	EOG091G0A54
TreeFamⁱ	TF313938

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-MMU-4641258 Degradation of DVL

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q8K4P8
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000021301 Expressed in 112 organ(s), highest expression level in retina
ExpressionAtlasⁱ	Q8K4P8 baseline and differential
Genevisibleⁱ	Q8K4P8 MM

Family and domain databases

CDDⁱ	cd08691 C2_NEDL1-like, 1 hit cd00078 HECTc, 1 hit cd00201 WW, 2 hits
Gene3Dⁱ	2.60.40.150, 1 hit
InterProⁱ	View protein in InterPro IPR000008 C2_dom IPR035892 C2_domain_sf IPR037795 C2_HECW IPR000569 HECT_dom IPR035983 Hect_E3_ubiquitin_ligase IPR032348 HECW_N IPR001202 WW_dom IPR036020 WW_dom_sf
Pfamⁱ	View protein in Pfam PF00168 C2, 1 hit PF00632 HECT, 1 hit PF16562 HECW_N, 1 hit PF00397 WW, 1 hit
SMARTⁱ	View protein in SMART SM00239 C2, 1 hit SM00119 HECTc, 1 hit SM00456 WW, 2 hits
SUPFAMⁱ	SSF51045 SSF51045, 2 hits SSF56204 SSF56204, 1 hit
PROSITEⁱ	View protein in PROSITE PS50004 C2, 1 hit PS50237 HECT, 1 hit PS01159 WW_DOMAIN_1, 2 hits PS50020 WW_DOMAIN_2, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HECW1_MOUSE
Accessionⁱ	Q8K4P8Primary (citable) accession number: Q8K4P8 Secondary accession number(s): D3YZ67 , Q6A086, Q8BIA6, Q8BKC2, Q8BKL3, Q8BKZ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
	Last sequence update:	July 27, 2011
	Last modified:	December 5, 2018
	This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

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UniRef

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Supporting data

UniProtKB - Q8K4P8 (HECW1_MOUSE)

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E3 ubiquitin-protein ligase HECW1

Hecw1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Catalytic activityⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ