UniProtKB - Q8K4P8 (HECW1_MOUSE)
Protein
E3 ubiquitin-protein ligase HECW1
Gene
Hecw1
Organism
Mus musculus (Mouse)
Status
Functioni
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1.By similarity1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1572 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- negative regulation of sodium ion transmembrane transporter activity Source: GO_Central
- positive regulation of protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
- protein ubiquitination Source: GO_Central
- regulation of dendrite morphogenesis Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-4641258, Degradation of DVL |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase HECW1 (EC:2.3.2.26)Alternative name(s): HECT, C2 and WW domain-containing protein 1 HECT-type E3 ubiquitin transferase HECW1 NEDD4-like E3 ubiquitin-protein ligase 1 Short name: mNEDL1 |
Gene namesi | Name:Hecw1 Synonyms:Kiaa0322, Nedl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2444115, Hecw1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000277666 | 1 – 1604 | E3 ubiquitin-protein ligase HECW1Add BLAST | 1604 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 871 | PhosphoserineCombined sources | 1 | |
Modified residuei | 935 | PhosphoserineCombined sources | 1 | |
Modified residuei | 937 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8K4P8 |
PeptideAtlasi | Q8K4P8 |
PRIDEi | Q8K4P8 |
PTM databases
iPTMneti | Q8K4P8 |
PhosphoSitePlusi | Q8K4P8 |
Expressioni
Tissue specificityi
Predominantly expressed in neurons of the spinal cord.
Gene expression databases
Bgeei | ENSMUSG00000021301, Expressed in lumbar subsegment of spinal cord and 133 other tissues |
Genevisiblei | Q8K4P8, MM |
Interactioni
Subunit structurei
Interacts with DVL1 and SSR3.
By similarityProtein-protein interaction databases
BioGRIDi | 220495, 2 interactors |
IntActi | Q8K4P8, 1 interactor |
STRINGi | 10090.ENSMUSP00000106145 |
Miscellaneous databases
RNActi | Q8K4P8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 182 – 318 | C2PROSITE-ProRule annotationAdd BLAST | 137 | |
Domaini | 826 – 859 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 1016 – 1049 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 1269 – 1604 | HECTPROSITE-ProRule annotationAdd BLAST | 336 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 871 – 898 | Sequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 407 – 524 | Glu-richAdd BLAST | 118 | |
Compositional biasi | 671 – 703 | Cys-richAdd BLAST | 33 |
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0940, Eukaryota |
GeneTreei | ENSGT00940000158294 |
HOGENOMi | CLU_002173_14_0_1 |
InParanoidi | Q8K4P8 |
OrthoDBi | 117748at2759 |
TreeFami | TF313938 |
Family and domain databases
CDDi | cd08691, C2_NEDL1-like, 1 hit cd00078, HECTc, 1 hit cd00201, WW, 2 hits |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR037795, C2_HECW IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR040524, HECW1_helix IPR032348, HECW_N IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF00632, HECT, 1 hit PF18436, HECW1_helix, 1 hit PF16562, HECW_N, 1 hit PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00119, HECTc, 1 hit SM00456, WW, 2 hits |
SUPFAMi | SSF51045, SSF51045, 2 hits SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS50237, HECT, 1 hit PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketNote: Named isoforms=2.
This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8K4P8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN
60 70 80 90 100
IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW
110 120 130 140 150
DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY
160 170 180 190 200
FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG
210 220 230 240 250
SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE
260 270 280 290 300
RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK
310 320 330 340 350
LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHADD
360 370 380 390 400
EEISLSAEPE SSAETQDSIM NSMVGNSNGE PSGDATEFCK DAKPESPSEG
410 420 430 440 450
NGVNSSENQN QEHAGPVEEA AGAMEARDGS NVSEAPEEPG ELQDPEQHDT
460 470 480 490 500
QPTLSAEEVA EGLPLDEDSP SSLLPEENTA LGSKVEEETV PENGAREEEM
510 520 530 540 550
QKGKDEEEEE EDVSTLEQGE PGLELRVSVR KKSRPCSLPV SELETVIASA
560 570 580 590 600
CGDAETPRTH YIRIHTLLHS MPSAQRGSTT EEEDGLEEES TLKESSEKDG
610 620 630 640 650
LSEVDTIAAD PQSMEDGESD GATLCMAPSD CSGGHFSSLS KGIGAGQDGE
660 670 680 690 700
AHPSTGSESD SSPQQGADHS CEGCDASCCS PSCYSTSCYS SSCYSSSCYS
710 720 730 740 750
SSCYNGNNRF ASHTRFSSVD SAKISESTVF SSQEDEEEEN SAFESVPDSV
760 770 780 790 800
QSPELDPEST NGAGPWQDEL AAPGGNAARS TEGLESPMAG PSNRREGECP
810 820 830 840 850
ILHNSQPISQ LPSLRPEHHH YPTIDEPLPP NWEARIDSHG RVFYVDHINR
860 870 880 890 900
TTTWQRPSMA PTPDGMIRSG SVHQMEQLNR RYQNIQRTMA TERAEEDSGN
910 920 930 940 950
QNSEQIPDGG GGGGGGSDSE AESSQSSLDL RREGSLSPVN SQKVTLLLQS
960 970 980 990 1000
PAVKFITNPE FFTVLHANYS AYRVFTSSTC LKHMILKVRR DARNFERYQH
1010 1020 1030 1040 1050
NRDLVNFINM FADTRLELPR GWEIKTDHQG KSFFVDHNSR ATTFIDPRIP
1060 1070 1080 1090 1100
LQNGRLPNHL THRQHLQRLR SYSAGEASEV SRNRGASLLA RPGHSLIAAI
1110 1120 1130 1140 1150
RSQHQHESLP LAYNDKIVAF LRQPNIFEML QERQPSLARN HTLREKIHYI
1160 1170 1180 1190 1200
RTEGNHGLDK LSCDADLVIL LSLFEEEIMS YVPLQSAFHP GYSFSPRCSP
1210 1220 1230 1240 1250
CSSPQNSPGL QRASARAPSP YRRDFEAKLR NFYRKLEAKG FGQGPGKIKL
1260 1270 1280 1290 1300
IIRRDHLLEG TFNQVMAYSR KELQRNKLYI TFVGEEGLDY SGPSREFFFL
1310 1320 1330 1340 1350
LSQELFNPYY GLFEYSANDT YTVQISPMSA FVENYLEWFR FSGRILGLAL
1360 1370 1380 1390 1400
IHQYLLDAFF TRPFYKGLLK LPCDLSDLEY LDEEFHQSLQ WMKDNNITDI
1410 1420 1430 1440 1450
LDLTFTVNEE VFGQVTEREL KSGGANTQVT EKNKKEYIER MVKWRVERGV
1460 1470 1480 1490 1500
VQQTEALLRG FYEVVDSRLV SVFDARELEL VIAGTAEIDL NDWRNNTEYR
1510 1520 1530 1540 1550
GGYHDGHLVI RWFWAAVERF NNEQRLRLLQ FVTGTSSVPY EGFAALRGSN
1560 1570 1580 1590 1600
GLRRFCIEKW GKITSLPRAH TCFNRLDLPP YPSYSMLYEK LLTAVEETST
FGLE
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VJ05 | A0A1Y7VJ05_MOUSE | HECT-type E3 ubiquitin transferase | Hecw1 | 1,177 | Annotation score: | ||
A0A1Y7VJ41 | A0A1Y7VJ41_MOUSE | E3 ubiquitin-protein ligase HECW1 | Hecw1 | 132 | Annotation score: | ||
A0A1Y7VJB7 | A0A1Y7VJB7_MOUSE | E3 ubiquitin-protein ligase HECW1 | Hecw1 | 74 | Annotation score: | ||
A0A1Y7VMF2 | A0A1Y7VMF2_MOUSE | E3 ubiquitin-protein ligase HECW1 | Hecw1 | 23 | Annotation score: |
Sequence cautioni
The sequence BAC28516 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 167 | S → R in BAB97389 (PubMed:14684739).Curated | 1 | |
Sequence conflicti | 823 | T → A in BAB97389 (PubMed:14684739).Curated | 1 | |
Sequence conflicti | 1036 | D → G in BAB97389 (PubMed:14684739).Curated | 1 | |
Sequence conflicti | 1248 | I → V in BAB97389 (PubMed:14684739).Curated | 1 | |
Sequence conflicti | 1356 | L → P in BAB97389 (PubMed:14684739).Curated | 1 | |
Sequence conflicti | 1538 | V → M in BAB97389 (PubMed:14684739).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023074 | 1 – 8 | MLLHLCSV → MICAQLEAA in isoform 3. 1 Publication | 8 | |
Alternative sequenceiVSP_023075 | 316 – 1604 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 1289 | |
Alternative sequenceiVSP_023076 | 782 – 795 | Missing in isoform 2. 1 PublicationAdd BLAST | 14 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK033922 mRNA Translation: BAC28516.1 Different initiation. AK047678 mRNA Translation: BAC33122.1 AK051569 mRNA Translation: BAC34677.1 AK053694 mRNA Translation: BAC35477.1 AC092710 Genomic DNA No translation available. AC154511 Genomic DNA No translation available. AC154615 Genomic DNA No translation available. AC161249 Genomic DNA No translation available. CT025604 Genomic DNA No translation available. AB083710 mRNA Translation: BAB97389.1 AK172932 mRNA Translation: BAD32210.1 |
CCDSi | CCDS49207.1 [Q8K4P8-1] |
RefSeqi | NP_001074817.3, NM_001081348.3 [Q8K4P8-1] XP_006516873.1, XM_006516810.3 [Q8K4P8-1] XP_006516874.1, XM_006516811.1 [Q8K4P8-1] XP_006516875.1, XM_006516812.3 [Q8K4P8-1] |
Genome annotation databases
Ensembli | ENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301 [Q8K4P8-1] |
GeneIDi | 94253 |
KEGGi | mmu:94253 |
UCSCi | uc007pnd.2, mouse [Q8K4P8-1] uc007png.2, mouse [Q8K4P8-4] uc007pnh.2, mouse [Q8K4P8-3] uc011ywk.1, mouse [Q8K4P8-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK033922 mRNA Translation: BAC28516.1 Different initiation. AK047678 mRNA Translation: BAC33122.1 AK051569 mRNA Translation: BAC34677.1 AK053694 mRNA Translation: BAC35477.1 AC092710 Genomic DNA No translation available. AC154511 Genomic DNA No translation available. AC154615 Genomic DNA No translation available. AC161249 Genomic DNA No translation available. CT025604 Genomic DNA No translation available. AB083710 mRNA Translation: BAB97389.1 AK172932 mRNA Translation: BAD32210.1 |
CCDSi | CCDS49207.1 [Q8K4P8-1] |
RefSeqi | NP_001074817.3, NM_001081348.3 [Q8K4P8-1] XP_006516873.1, XM_006516810.3 [Q8K4P8-1] XP_006516874.1, XM_006516811.1 [Q8K4P8-1] XP_006516875.1, XM_006516812.3 [Q8K4P8-1] |
3D structure databases
SMRi | Q8K4P8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 220495, 2 interactors |
IntActi | Q8K4P8, 1 interactor |
STRINGi | 10090.ENSMUSP00000106145 |
PTM databases
iPTMneti | Q8K4P8 |
PhosphoSitePlusi | Q8K4P8 |
Proteomic databases
PaxDbi | Q8K4P8 |
PeptideAtlasi | Q8K4P8 |
PRIDEi | Q8K4P8 |
Protocols and materials databases
Antibodypediai | 1996, 44 antibodies |
DNASUi | 94253 |
Genome annotation databases
Ensembli | ENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301 [Q8K4P8-1] |
GeneIDi | 94253 |
KEGGi | mmu:94253 |
UCSCi | uc007pnd.2, mouse [Q8K4P8-1] uc007png.2, mouse [Q8K4P8-4] uc007pnh.2, mouse [Q8K4P8-3] uc011ywk.1, mouse [Q8K4P8-2] |
Organism-specific databases
CTDi | 23072 |
MGIi | MGI:2444115, Hecw1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0940, Eukaryota |
GeneTreei | ENSGT00940000158294 |
HOGENOMi | CLU_002173_14_0_1 |
InParanoidi | Q8K4P8 |
OrthoDBi | 117748at2759 |
TreeFami | TF313938 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-4641258, Degradation of DVL |
Miscellaneous databases
BioGRID-ORCSi | 94253, 1 hit in 17 CRISPR screens |
PROi | PR:Q8K4P8 |
RNActi | Q8K4P8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021301, Expressed in lumbar subsegment of spinal cord and 133 other tissues |
Genevisiblei | Q8K4P8, MM |
Family and domain databases
CDDi | cd08691, C2_NEDL1-like, 1 hit cd00078, HECTc, 1 hit cd00201, WW, 2 hits |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR037795, C2_HECW IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR040524, HECW1_helix IPR032348, HECW_N IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF00632, HECT, 1 hit PF18436, HECW1_helix, 1 hit PF16562, HECW_N, 1 hit PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00119, HECTc, 1 hit SM00456, WW, 2 hits |
SUPFAMi | SSF51045, SSF51045, 2 hits SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS50237, HECT, 1 hit PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HECW1_MOUSE | |
Accessioni | Q8K4P8Primary (citable) accession number: Q8K4P8 Secondary accession number(s): D3YZ67 Q8BKZ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways