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Protein

MKL/myocardin-like protein 1

Gene

Mkl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • actin monomer binding Source: UniProtKB
  • DNA binding transcription factor activity Source: MGI
  • leucine zipper domain binding Source: MGI
  • transcription coactivator activity Source: UniProtKB
  • transcription regulatory region sequence-specific DNA binding Source: MGI

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • forebrain development Source: MGI
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • neuron migration Source: MGI
  • neuron projection development Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • positive regulation of transcription via serum response element binding Source: UniProtKB
  • regulation of transcription by RNA polymerase II Source: MGI
  • smooth muscle cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActin-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
MKL/myocardin-like protein 1
Alternative name(s):
Basic SAP coiled-coil transcription activator
Megakaryoblastic leukemia 1 protein homolog
Myocardin-related transcription factor A
Short name:
MRTF-A
Gene namesi
Name:Mkl1
Synonyms:Bsac
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2384495 Mkl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266261 – 964MKL/myocardin-like protein 1Add BLAST964

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineBy similarity1
Modified residuei191PhosphoserineBy similarity1
Modified residuei371PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei488PhosphothreonineCombined sources1
Modified residuei492PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K4J6
PaxDbiQ8K4J6
PRIDEiQ8K4J6

PTM databases

iPTMnetiQ8K4J6
PhosphoSitePlusiQ8K4J6

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis.

Developmental stagei

Detected throughout the embryo at 10.5 dpc; higher expression is found at 13.5 dpc in neural mesenchymal cells, skeletal muscle of the tongue, and epithelial cells of the colon and small intestine; at 15.5 dpc, expression in epithelial cells of lung, kidney, bladder, and colon is also detected.

Gene expression databases

BgeeiENSMUSG00000042292
CleanExiMM_MKL1
ExpressionAtlasiQ8K4J6 baseline and differential
GenevisibleiQ8K4J6 MM

Interactioni

Subunit structurei

Forms with SCAI and SRF a nuclear ternary complex which binds the CArG consensus motif (CArG box) on DNA via SRF. Some authors (PubMed:12019265) have found contradictory results, they could not demonstrate that it forms a complex with the CArG boxes, even in the presence of SRF. Interacts with ACTB; interaction with ACTB prevents interaction with SCAI. Interacts with MKL2.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: MGI
  • actin monomer binding Source: UniProtKB
  • leucine zipper domain binding Source: MGI

Protein-protein interaction databases

BioGridi230180, 2 interactors
DIPiDIP-60884N
ELMiQ8K4J6
IntActiQ8K4J6, 6 interactors
MINTiQ8K4J6
STRINGi10090.ENSMUSP00000105207

Structurei

Secondary structure

1964
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 23Combined sources9
Helixi27 – 32Combined sources6
Beta strandi39 – 41Combined sources3
Helixi59 – 66Combined sources8
Helixi71 – 76Combined sources6
Helixi87 – 110Combined sources24
Helixi115 – 120Combined sources6

3D structure databases

ProteinModelPortaliQ8K4J6
SMRiQ8K4J6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4J6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati15 – 40RPEL 1Add BLAST26
Repeati59 – 84RPEL 2Add BLAST26
Repeati103 – 128RPEL 3Add BLAST26
Domaini385 – 419SAPPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 291Mediates interaction with SCAI and ACTB1 PublicationAdd BLAST291

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili552 – 600Sequence analysisAdd BLAST49

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi299 – 325Gln-richAdd BLAST27
Compositional biasi712 – 844Pro-richAdd BLAST133

Domaini

The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
The RPEL repeats mediate binding to globular actin.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IFJX Eukaryota
ENOG41101AM LUCA
GeneTreeiENSGT00530000063195
HOGENOMiHOG000038001
HOVERGENiHBG036493
InParanoidiQ8K4J6
KOiK22525
OMAiAQMDLEH
OrthoDBiEOG091G01ZY
PhylomeDBiQ8K4J6
TreeFamiTF326024

Family and domain databases

Gene3Di1.10.720.30, 1 hit
InterProiView protein in InterPro
IPR029992 MRTF-A
IPR004018 RPEL_repeat
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR22793:SF6 PTHR22793:SF6, 2 hits
PfamiView protein in Pfam
PF02755 RPEL, 3 hits
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00707 RPEL, 3 hits
SM00513 SAP, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51073 RPEL, 3 hits
PS50800 SAP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4J6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLLEPEMLM MAVQSVLQLK LQQRRTREEL VSQGIMPPLK SPAAFHEQRR
60 70 80 90 100
SLERARTEDY LKRKIRSRPE RAELVRMHIL EETSAEPSLQ AKQLKLKRAR
110 120 130 140 150
LADDLNEKIA QRPGPMELVE KNILPVESSL KEAIIVGQVN YPKVADSSSF
160 170 180 190 200
DEDSSDALSP EQPASHESQG SVPSPLESRV SDPLPSATSI SPTQVLSQLP
210 220 230 240 250
MAPDPGETLF LAEQPPLPPA PLLPPSLANG SIVPTAKPAP TLIKQSQPKS
260 270 280 290 300
ASEKSQRSKK AKELKPKVKK LKYHQYIPPD QKQDKGAPAM DSSYAKILQQ
310 320 330 340 350
QQLFLQLQIL NQQQQQQQQQ HYNYQAILPA PPKPSAETPG SSAPTPSRSL
360 370 380 390 400
STSSSPSSGT PGPSGLARQS STALAAKPGA LPANLDDMKV AELKQELKLR
410 420 430 440 450
SLPVSGTKTE LIERLRAYQD QVSPAPGAPK APATTSVLSK AGEVVVAFPA
460 470 480 490 500
ALLSTGSALV TAGLAPAEMV VATVTSNGMV KFGSTGSTPP VSPTPSERSL
510 520 530 540 550
LSTGDENSTP GDAFGEMVTS PLTQLTLQAS PLQIVKEEGA RAASCCLSPG
560 570 580 590 600
ARAELEGLDK DQMLQEKDKQ IEELTRMLQQ KQQLVELLRL QLEQQKRAQQ
610 620 630 640 650
PAPASSPVKR ESGFSSCQLS CQPQGSAHAF GSGLVVPTTN HGDTQAPAPE
660 670 680 690 700
SPPVVVKQEA GPPEPDLAPS SQLLLGSQGT SFLKRVSPPT LVTDSTGTHL
710 720 730 740 750
ILTVTNKSAD GPGLPAGSPQ QPLSQPGSPA PGPPAQMDLE HPPQPPFATP
760 770 780 790 800
TSLLKKEPPG YEETVTQQPK QQENGSSSQH MDDLFDILIQ SGEISADFKE
810 820 830 840 850
PPSLPGKEKS PPAAAAYGPP LTPQPSPLSE LPQAAPPPGS PTLPGRLEDF
860 870 880 890 900
LESSTGLPLL TSGHEGPEPL SLIDDLHSQM LSSSAILDHP PSPMDTSELH
910 920 930 940 950
FAPEPSSGMG LDLAVGHLDS MDWLELSSGG PVLSLAPLST AAPSLFSMDF
960
LDGHDLQLHW DSCL
Length:964
Mass (Da):102,546
Last modified:June 27, 2003 - v2
Checksum:iAFAEA328A1860CE5
GO
Isoform 2 (identifier: Q8K4J6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Show »
Length:929
Mass (Da):98,463
Checksum:iB13D0985013B6E49
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53E → Q in BAC31809 (PubMed:16141072).Curated1
Sequence conflicti724S → D in BAC40873 (PubMed:16141072).Curated1
Sequence conflicti728S → F in AAM94258 (PubMed:12019265).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0076521 – 35Missing in isoform 2. 3 PublicationsAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385582 mRNA Translation: AAM94258.1
AF532597 mRNA Translation: AAN33041.1
AK044188 mRNA Translation: BAC31809.1
AK089416 mRNA Translation: BAC40873.1
BC050941 mRNA Translation: AAH50941.1
CCDSiCCDS37147.1 [Q8K4J6-1]
CCDS49674.1 [Q8K4J6-2]
RefSeqiNP_001076005.1, NM_001082536.1 [Q8K4J6-2]
NP_694629.2, NM_153049.3 [Q8K4J6-1]
UniGeneiMm.439814

Genome annotation databases

EnsembliENSMUST00000109579; ENSMUSP00000105207; ENSMUSG00000042292 [Q8K4J6-1]
ENSMUST00000134469; ENSMUSP00000119530; ENSMUSG00000042292 [Q8K4J6-2]
ENSMUST00000149582; ENSMUSP00000117745; ENSMUSG00000042292 [Q8K4J6-2]
GeneIDi223701
KEGGimmu:223701
UCSCiuc007wwc.2 mouse [Q8K4J6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMKL1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4J6
Secondary accession number(s): Q642U1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 27, 2003
Last modified: July 18, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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