UniProtKB - Q8K4J6 (MRTFA_MOUSE)

BLAST

>sp|Q8K4J6|MRTFA_MOUSE Myocardin-related transcription factor A OS=Mus musculus OX=10090 GN=Mrtfa PE=1 SV=2
MTLLEPEMLMMAVQSVLQLKLQQRRTREELVSQGIMPPLKSPAAFHEQRRSLERARTEDY
LKRKIRSRPERAELVRMHILEETSAEPSLQAKQLKLKRARLADDLNEKIAQRPGPMELVE
KNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSPLESRV
SDPLPSATSISPTQVLSQLPMAPDPGETLFLAEQPPLPPAPLLPPSLANGSIVPTAKPAP
TLIKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDKGAPAMDSSYAKILQQ
QQLFLQLQILNQQQQQQQQQHYNYQAILPAPPKPSAETPGSSAPTPSRSLSTSSSPSSGT
PGPSGLARQSSTALAAKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQD
QVSPAPGAPKAPATTSVLSKAGEVVVAFPAALLSTGSALVTAGLAPAEMVVATVTSNGMV
KFGSTGSTPPVSPTPSERSLLSTGDENSTPGDAFGEMVTSPLTQLTLQASPLQIVKEEGA
RAASCCLSPGARAELEGLDKDQMLQEKDKQIEELTRMLQQKQQLVELLRLQLEQQKRAQQ
PAPASSPVKRESGFSSCQLSCQPQGSAHAFGSGLVVPTTNHGDTQAPAPESPPVVVKQEA
GPPEPDLAPSSQLLLGSQGTSFLKRVSPPTLVTDSTGTHLILTVTNKSADGPGLPAGSPQ
QPLSQPGSPAPGPPAQMDLEHPPQPPFATPTSLLKKEPPGYEETVTQQPKQQENGSSSQH
MDDLFDILIQSGEISADFKEPPSLPGKEKSPPAAAAYGPPLTPQPSPLSELPQAAPPPGS
PTLPGRLEDFLESSTGLPLLTSGHEGPEPLSLIDDLHSQMLSSSAILDHPPSPMDTSELH
FAPEPSSGMGLDLAVGHLDSMDWLELSSGGPVLSLAPLSTAAPSLFSMDFLDGHDLQLHW
DSCL

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History
Entry version 148 (07 Nov 2018)
Sequence version 2 (27 Jun 2003)
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Protein

Myocardin-related transcription factor A

Gene

Mrtfa

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381).7 Publications

Caution

Some publications use a protein sequence that is longer at the N-terminus and is based on an artificial construct (PubMed:12732141, PubMed:27304076). The sequence used in these publications modifies a non-canonical CTG leucine codon upstream of the initiator codon into ATG, generating a protein of 1021 residues (PubMed:12732141, PubMed:27304076). The existence of this form has not been confirmed in vivo and is therefore unsure (PubMed:12732141, PubMed:27304076). Similarly, the existence of the S33 ('Ser-33') phosphorylation site described in Panayiotou et al. is unsure (PubMed:27304076).2 Publications

GO - Molecular functionⁱ

actin binding
Source: MGI
Inferred from direct assayⁱ
- 15798203
actin monomer binding
Source: UniProtKB
Inferred from direct assayⁱ
DNA-binding transcription factor activity
Source: MGI
Inferred from direct assayⁱ
- 17991879
leucine zipper domain binding Source: MGI
transcription coactivator activity
Source: UniProtKB
Inferred from direct assayⁱ
transcription regulatory region sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 17991879

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin cytoskeleton organization
Source: UniProtKB
Inferred from direct assayⁱ
- 24732378
forebrain development
Source: MGI
Inferred from genetic interactionⁱ
- 20534669
negative regulation of apoptotic signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 12019265
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
Source: MGI
Inferred from direct assayⁱ
- 12019265
neuron migration
Source: MGI
Inferred from genetic interactionⁱ
- 20534669
neuron projection development
Source: MGI
Inferred from genetic interactionⁱ
- 20534669
positive regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 12019265
- 15798203
positive regulation of transcription by RNA polymerase II
Source: UniProtKB
Inferred from direct assayⁱ
- 21984612
positive regulation of transcription via serum response element binding
Source: UniProtKB
Inferred from direct assayⁱ
regulation of transcription by RNA polymerase II Source: MGI
smooth muscle cell differentiation Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Actin-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-3899300 SUMOylation of transcription cofactors R-MMU-5663220 RHO GTPases Activate Formins

Reactomeⁱ

R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Myocardin-related transcription factor ACurated Short name: MRTF-A1 Publication Alternative name(s): Basic SAP coiled-coil transcription activator1 Publication MKL/myocardin-like protein 1 Megakaryoblastic leukemia 1 protein homolog Megakaryocytic acute leukemia protein homolog2 Publications
Gene namesⁱ	Name:Mrtfa Synonyms:Bsac1 Publication, Mal2 Publications, Mkl1Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 15

Organism-specific databases

MGIⁱ	MGI:2384495 Mkl1

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	24	R → A in 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-68 and A-112. 1 Publication	1
Mutagenesisⁱ	45	F → A or D: Induces a nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	48	Q → A or D: Induces a nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	52	L → A or D: Induces a nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	54	R → A: Impaired interaction with G-actin, leading to nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	56	R → A: Impaired interaction with G-actin, leading to nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	68	R → A in 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-24 and A-112. 1 Publication	1
Mutagenesisⁱ	89	L → A or D: Does not induce a nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	92	K → A or D: Does not induce a nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	96	L → A: Induces a nuclear accumulation in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	96	L → D: Induces a nuclear decrease in unstimulated cells. 1 Publication	1
Mutagenesisⁱ	98	R → A: Impaired interaction with G-actin, leading to cytoplasmic accumulation. 1 Publication	1
Mutagenesisⁱ	100	R → A: Impaired interaction with G-actin, leading to cytoplasmic accumulation. 1 Publication	1
Mutagenesisⁱ	112	R → A in 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-24 and A-68. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000126626	1 – 964	Myocardin-related transcription factor AAdd BLAST		964

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	41	Phosphoserine1 Publication	1
Modified residueⁱ	159	Phosphoserine1 Publication	1
Modified residueⁱ	174	Phosphoserine1 Publication	1
Modified residueⁱ	191	Phosphoserine1 Publication	1
Modified residueⁱ	349	Phosphoserine1 Publication	1
Modified residueⁱ	351	Phosphoserine1 Publication	1
Modified residueⁱ	352	Phosphothreonine1 Publication	1
Modified residueⁱ	355	Phosphoserine1 Publication	1
Modified residueⁱ	358	Phosphoserine1 Publication	1
Modified residueⁱ	360	Phosphothreonine1 Publication	1
Modified residueⁱ	371	PhosphoserineBy similarity	1
Modified residueⁱ	423	Phosphoserine1 Publication	1
Modified residueⁱ	484	Phosphoserine1 Publication	1
Modified residueⁱ	485	Phosphothreonine1 Publication	1
Modified residueⁱ	487	Phosphoserine1 Publication	1
Modified residueⁱ	488	Phosphothreonine1 Publication	1
Modified residueⁱ	492	Phosphoserine1 Publication	1
Modified residueⁱ	494	Phosphothreonine1 Publication	1
Modified residueⁱ	496	Phosphoserine1 Publication	1
Modified residueⁱ	520	Phosphoserine1 Publication	1
Modified residueⁱ	530	Phosphoserine1 Publication	1
Modified residueⁱ	544	Phosphoserine1 Publication	1
Modified residueⁱ	548	Phosphoserine1 Publication	1
Modified residueⁱ	605	Phosphoserine1 Publication	1
Modified residueⁱ	606	Phosphoserine1 Publication	1
Modified residueⁱ	651	Phosphoserine1 Publication	1
Modified residueⁱ	687	Phosphoserine1 Publication	1
Modified residueⁱ	718	Phosphoserine1 Publication	1
Modified residueⁱ	724	Phosphoserine1 Publication	1
Modified residueⁱ	728	Phosphoserine1 Publication	1
Modified residueⁱ	810	Phosphoserine1 Publication	1
Modified residueⁱ	822	Phosphothreonine1 Publication	1
Modified residueⁱ	826	Phosphoserine1 Publication	1
Modified residueⁱ	840	Phosphoserine1 Publication	1
Modified residueⁱ	842	Phosphothreonine1 Publication	1
Modified residueⁱ	892	Phosphoserine1 Publication	1

Post-translational modificationⁱ

Phosphorylation at Ser-41 by Erk inhibits binding of globular actin (G-actin), unmasking the nuclear localization signal (NLS) and promoting nuclear import.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q8K4J6
PaxDbⁱ	Q8K4J6
PRIDEⁱ	Q8K4J6

PTM databases

iPTMnetⁱ	Q8K4J6
PhosphoSitePlusⁱ	Q8K4J6

Expressionⁱ

Tissue specificityⁱ

Expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis.1 Publication

Developmental stageⁱ

Detected throughout the embryo at 10.5 dpc; higher expression is found at 13.5 dpc in neural mesenchymal cells, skeletal muscle of the tongue, and epithelial cells of the colon and small intestine; at 15.5 dpc, expression in epithelial cells of lung, kidney, bladder, and colon is also detected.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000042292 Expressed in 277 organ(s), highest expression level in dentate gyrus granule cell layer
CleanExⁱ	MM_MKL1
ExpressionAtlasⁱ	Q8K4J6 baseline and differential
Genevisibleⁱ	Q8K4J6 MM

Interactionⁱ

Subunit structureⁱ

Interacts with SRF, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF (PubMed:12732141, PubMed:19350017). Interacts (via RPEL repeats) with globular actin (G-actin), thereby regulating its subcellular location and activity of the complex formed with SRF (PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:19008859, PubMed:27304076, PubMed:21673315). Either forms a trivalent (by binding three G-actin monomers) or pentavalent (by binding five G-actin monomers) complex with G-actin (PubMed:21673315). Forms a nuclear ternary complex with SCAI and SRF, leading to suppress MRTFA-induced SRF transcriptional activity (PubMed:19350017). Interacts with beta-actin (ACTB); interaction with ACTB prevents interaction with SCAI (PubMed:19350017). Interacts with MRTFB (By similarity).By similarity6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ACTA1	P68135	15	EBI-8291665,EBI-367540	From Oryctolagus cuniculus.
ACTB	P60709	3	EBI-8291665,EBI-353944	From Homo sapiens.
KPNA4	O00629	4	EBI-8291665,EBI-396343	From Homo sapiens.
KPNB1	Q14974	2	EBI-8291665,EBI-286758	From Homo sapiens.

GO - Molecular functionⁱ

actin binding
Source: MGI
Inferred from direct assayⁱ
- 15798203
actin monomer binding
Source: UniProtKB
Inferred from direct assayⁱ
leucine zipper domain binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	230180, 2 interactors
Database of interacting proteins More...DIPⁱ	DIP-60884N
ELMⁱ	Q8K4J6
IntActⁱ	Q8K4J6, 6 interactors
Molecular INTeraction database More...MINTⁱ	Q8K4J6
STRINGⁱ	10090.ENSMUSP00000105207

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	15 – 23	Combined sources	9
Helixⁱ	27 – 32	Combined sources	6
Beta strandⁱ	39 – 41	Combined sources	3
Helixⁱ	59 – 66	Combined sources	8
Helixⁱ	71 – 76	Combined sources	6
Helixⁱ	87 – 110	Combined sources	24
Helixⁱ	115 – 120	Combined sources	6

Show more details

3D structure databases

ProteinModelPortalⁱ	Q8K4J6
SMRⁱ	Q8K4J6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q8K4J6

EvolutionaryTraceⁱ

Q8K4J6

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	15 – 40	RPEL 1Add BLAST	26
Repeatⁱ	59 – 84	RPEL 2Add BLAST	26
Repeatⁱ	103 – 128	RPEL 3Add BLAST	26
Domainⁱ	385 – 419	SAPPROSITE-ProRule annotationAdd BLAST	35

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 291	Mediates interaction with SCAI and ACTB1 PublicationAdd BLAST	291
Regionⁱ	41 – 58	Intervening spacer sequence 11 PublicationAdd BLAST	18
Regionⁱ	85 – 102	Intervening spacer sequence 21 PublicationAdd BLAST	18

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	552 – 600	Sequence analysisAdd BLAST		49

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	299 – 325	Gln-richAdd BLAST		27
Compositional biasⁱ	712 – 844	Pro-richAdd BLAST		133

Domainⁱ

The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.1 Publication

The RPEL repeats mediate binding to globular actin (G-actin); each RPEL repeat-binding to one G-actin monomer (PubMed:19008859, PubMed:21673315). In addition, each intervening spacer sequence region can bind one G-actin monomer, to reach a pentavalent complex (PubMed:21673315).2 Publications

Keywords - Domainⁱ

Coiled coil, Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410IFJX Eukaryota ENOG41101AM LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063195
HOGENOMⁱ	HOG000038001
HOVERGENⁱ	HBG036493
InParanoidⁱ	Q8K4J6
KEGG Orthology (KO) More...KOⁱ	K22525
OMAⁱ	SELHFAH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G01ZY
PhylomeDBⁱ	Q8K4J6
TreeFamⁱ	TF326024

Family and domain databases

Gene3Dⁱ	1.10.720.30, 1 hit
InterProⁱ	View protein in InterPro IPR029992 MRTF-A IPR004018 RPEL_repeat IPR003034 SAP_dom IPR036361 SAP_dom_sf
PANTHERⁱ	PTHR22793:SF6 PTHR22793:SF6, 2 hits
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02755 RPEL, 3 hits PF02037 SAP, 1 hit
SMARTⁱ	View protein in SMART SM00707 RPEL, 3 hits SM00513 SAP, 1 hit
SUPFAMⁱ	SSF68906 SSF68906, 1 hit
PROSITEⁱ	View protein in PROSITE PS51073 RPEL, 3 hits PS50800 SAP, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q8K4J6-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLLEPEMLM MAVQSVLQLK LQQRRTREEL VSQGIMPPLK SPAAFHEQRR 
        60         70         80         90        100
SLERARTEDY LKRKIRSRPE RAELVRMHIL EETSAEPSLQ AKQLKLKRAR 
       110        120        130        140        150
LADDLNEKIA QRPGPMELVE KNILPVESSL KEAIIVGQVN YPKVADSSSF 
       160        170        180        190        200
DEDSSDALSP EQPASHESQG SVPSPLESRV SDPLPSATSI SPTQVLSQLP 
       210        220        230        240        250
MAPDPGETLF LAEQPPLPPA PLLPPSLANG SIVPTAKPAP TLIKQSQPKS 
       260        270        280        290        300
ASEKSQRSKK AKELKPKVKK LKYHQYIPPD QKQDKGAPAM DSSYAKILQQ 
       310        320        330        340        350
QQLFLQLQIL NQQQQQQQQQ HYNYQAILPA PPKPSAETPG SSAPTPSRSL 
       360        370        380        390        400
STSSSPSSGT PGPSGLARQS STALAAKPGA LPANLDDMKV AELKQELKLR 
       410        420        430        440        450
SLPVSGTKTE LIERLRAYQD QVSPAPGAPK APATTSVLSK AGEVVVAFPA 
       460        470        480        490        500
ALLSTGSALV TAGLAPAEMV VATVTSNGMV KFGSTGSTPP VSPTPSERSL 
       510        520        530        540        550
LSTGDENSTP GDAFGEMVTS PLTQLTLQAS PLQIVKEEGA RAASCCLSPG 
       560        570        580        590        600
ARAELEGLDK DQMLQEKDKQ IEELTRMLQQ KQQLVELLRL QLEQQKRAQQ 
       610        620        630        640        650
PAPASSPVKR ESGFSSCQLS CQPQGSAHAF GSGLVVPTTN HGDTQAPAPE 
       660        670        680        690        700
SPPVVVKQEA GPPEPDLAPS SQLLLGSQGT SFLKRVSPPT LVTDSTGTHL 
       710        720        730        740        750
ILTVTNKSAD GPGLPAGSPQ QPLSQPGSPA PGPPAQMDLE HPPQPPFATP 
       760        770        780        790        800
TSLLKKEPPG YEETVTQQPK QQENGSSSQH MDDLFDILIQ SGEISADFKE 
       810        820        830        840        850
PPSLPGKEKS PPAAAAYGPP LTPQPSPLSE LPQAAPPPGS PTLPGRLEDF 
       860        870        880        890        900
LESSTGLPLL TSGHEGPEPL SLIDDLHSQM LSSSAILDHP PSPMDTSELH 
       910        920        930        940        950
FAPEPSSGMG LDLAVGHLDS MDWLELSSGG PVLSLAPLST AAPSLFSMDF 
       960 
LDGHDLQLHW DSCL

Length:964

Mass (Da):102,546

Last modified:June 27, 2003 - v2

Checksum:ⁱAFAEA328A1860CE5

Isoform 2 (identifier: Q8K4J6-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-35: Missing.

« Hide

        10         20         30         40         50
MPPLKSPAAF HEQRRSLERA RTEDYLKRKI RSRPERAELV RMHILEETSA 
        60         70         80         90        100
EPSLQAKQLK LKRARLADDL NEKIAQRPGP MELVEKNILP VESSLKEAII 
       110        120        130        140        150
VGQVNYPKVA DSSSFDEDSS DALSPEQPAS HESQGSVPSP LESRVSDPLP 
       160        170        180        190        200
SATSISPTQV LSQLPMAPDP GETLFLAEQP PLPPAPLLPP SLANGSIVPT 
       210        220        230        240        250
AKPAPTLIKQ SQPKSASEKS QRSKKAKELK PKVKKLKYHQ YIPPDQKQDK 
       260        270        280        290        300
GAPAMDSSYA KILQQQQLFL QLQILNQQQQ QQQQQHYNYQ AILPAPPKPS 
       310        320        330        340        350
AETPGSSAPT PSRSLSTSSS PSSGTPGPSG LARQSSTALA AKPGALPANL 
       360        370        380        390        400
DDMKVAELKQ ELKLRSLPVS GTKTELIERL RAYQDQVSPA PGAPKAPATT 
       410        420        430        440        450
SVLSKAGEVV VAFPAALLST GSALVTAGLA PAEMVVATVT SNGMVKFGST 
       460        470        480        490        500
GSTPPVSPTP SERSLLSTGD ENSTPGDAFG EMVTSPLTQL TLQASPLQIV 
       510        520        530        540        550
KEEGARAASC CLSPGARAEL EGLDKDQMLQ EKDKQIEELT RMLQQKQQLV 
       560        570        580        590        600
ELLRLQLEQQ KRAQQPAPAS SPVKRESGFS SCQLSCQPQG SAHAFGSGLV 
       610        620        630        640        650
VPTTNHGDTQ APAPESPPVV VKQEAGPPEP DLAPSSQLLL GSQGTSFLKR 
       660        670        680        690        700
VSPPTLVTDS TGTHLILTVT NKSADGPGLP AGSPQQPLSQ PGSPAPGPPA 
       710        720        730        740        750
QMDLEHPPQP PFATPTSLLK KEPPGYEETV TQQPKQQENG SSSQHMDDLF 
       760        770        780        790        800
DILIQSGEIS ADFKEPPSLP GKEKSPPAAA AYGPPLTPQP SPLSELPQAA 
       810        820        830        840        850
PPPGSPTLPG RLEDFLESST GLPLLTSGHE GPEPLSLIDD LHSQMLSSSA 
       860        870        880        890        900
ILDHPPSPMD TSELHFAPEP SSGMGLDLAV GHLDSMDWLE LSSGGPVLSL 
       910        920 
APLSTAAPSL FSMDFLDGHD LQLHWDSCL

Show »

Length:929

Mass (Da):98,463

Checksum:ⁱB13D0985013B6E49

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

D3YUG5	D3YUG5_MOUSE	MKL (megakaryoblastic leukemia)/myo... MKL (megakaryoblastic leukemia)/myocardin-like 1	Mkl1	705	Annotation score:
D3YUI2	D3YUI2_MOUSE	MKL (megakaryoblastic leukemia)/myo... MKL (megakaryoblastic leukemia)/myocardin-like 1	Mkl1	879	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	53	E → Q in BAC31809 (PubMed:16141072).Curated	1
Sequence conflictⁱ	724	S → D in BAC40873 (PubMed:16141072).Curated	1
Sequence conflictⁱ	728	S → F in AAM94258 (PubMed:12019265).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_007652	1 – 35	Missing in isoform 2. 3 PublicationsAdd BLAST		35

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF385582 mRNA Translation: AAM94258.1 AF532597 mRNA Translation: AAN33041.1 AK044188 mRNA Translation: BAC31809.1 AK089416 mRNA Translation: BAC40873.1 BC050941 mRNA Translation: AAH50941.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37147.1 [Q8K4J6-1] CCDS49674.1 [Q8K4J6-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001076005.1, NM_001082536.1 [Q8K4J6-2] NP_694629.2, NM_153049.3 [Q8K4J6-1]
UniGeneⁱ	Mm.439814

Genome annotation databases

Ensemblⁱ	ENSMUST00000109579; ENSMUSP00000105207; ENSMUSG00000042292 [Q8K4J6-1] ENSMUST00000134469; ENSMUSP00000119530; ENSMUSG00000042292 [Q8K4J6-2] ENSMUST00000149582; ENSMUSP00000117745; ENSMUSG00000042292 [Q8K4J6-2]
GeneIDⁱ	223701
KEGGⁱ	mmu:223701
UCSC genome browser More...UCSCⁱ	uc007wwc.2 mouse [Q8K4J6-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8K4J6	Isoform 2 of Myocardin-related transcription factor A		MOUSE		929	UniRef100_Q8K4J6
MKL (megakaryoblastic leukemia)/myocardin-like 1 (Fragment)		MOUSE		705

Protein	Similar proteins		Species	Score	Length	Source
Q8K4J6	Isoform 2 of Myocardin-related transcription factor A		MOUSE		929	UniRef90_Q8K4J6
MKL/myocardin-like protein 1		CRIGR		1047
Megakaryoblastic leukemia (translocation) 1		RAT		1038
MKL/myocardin-like protein 1 isoform X1		MESAU		961
UPI00042AC22B		PERMB		1013
+20

Protein	Similar proteins		Species	Score	Length	Source
Q8K4J6	Myocardin-related transcription factor A	)	HUMAN		931	UniRef50_Q8K4J6
Isoform 2 of Myocardin-related transcription factor A		MOUSE		929
MKL/myocardin-like protein 1		CRIGR		1047
Megakaryoblastic leukemia (translocation) 1		RAT		1038
MKL/myocardin-like protein 1 isoform X1		MESAU		961
+294

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF385582 mRNA Translation: AAM94258.1 AF532597 mRNA Translation: AAN33041.1 AK044188 mRNA Translation: BAC31809.1 AK089416 mRNA Translation: BAC40873.1 BC050941 mRNA Translation: AAH50941.1
CCDSⁱ	CCDS37147.1 [Q8K4J6-1] CCDS49674.1 [Q8K4J6-2]
RefSeqⁱ	NP_001076005.1, NM_001082536.1 [Q8K4J6-2] NP_694629.2, NM_153049.3 [Q8K4J6-1]
UniGeneⁱ	Mm.439814

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2V51	X-ray	2.35	E/F	16-41	[»]
	2V52	X-ray	1.45	M	54-85	[»]
	2YJE	X-ray	3.10	M	16-142	[»]
	2YJF	X-ray	3.50	M	16-142	[»]
ProteinModelPortalⁱ	Q8K4J6
SMRⁱ	Q8K4J6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	230180, 2 interactors
DIPⁱ	DIP-60884N
ELMⁱ	Q8K4J6
IntActⁱ	Q8K4J6, 6 interactors
MINTⁱ	Q8K4J6
STRINGⁱ	10090.ENSMUSP00000105207

PTM databases

iPTMnetⁱ	Q8K4J6
PhosphoSitePlusⁱ	Q8K4J6

Proteomic databases

EPDⁱ	Q8K4J6
PaxDbⁱ	Q8K4J6
PRIDEⁱ	Q8K4J6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000109579; ENSMUSP00000105207; ENSMUSG00000042292 [Q8K4J6-1] ENSMUST00000134469; ENSMUSP00000119530; ENSMUSG00000042292 [Q8K4J6-2] ENSMUST00000149582; ENSMUSP00000117745; ENSMUSG00000042292 [Q8K4J6-2]
GeneIDⁱ	223701
KEGGⁱ	mmu:223701
UCSCⁱ	uc007wwc.2 mouse [Q8K4J6-1]

Organism-specific databases

CTDⁱ	223701
MGIⁱ	MGI:2384495 Mkl1

Phylogenomic databases

eggNOGⁱ	ENOG410IFJX Eukaryota ENOG41101AM LUCA
GeneTreeⁱ	ENSGT00530000063195
HOGENOMⁱ	HOG000038001
HOVERGENⁱ	HBG036493
InParanoidⁱ	Q8K4J6
KOⁱ	K22525
OMAⁱ	SELHFAH
OrthoDBⁱ	EOG091G01ZY
PhylomeDBⁱ	Q8K4J6
TreeFamⁱ	TF326024

Enzyme and pathway databases

Reactomeⁱ	R-MMU-3899300 SUMOylation of transcription cofactors R-MMU-5663220 RHO GTPases Activate Formins

Reactomeⁱ

R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-5663220 RHO GTPases Activate Formins

Miscellaneous databases

ChiTaRSⁱ	Mkl1 mouse
EvolutionaryTraceⁱ	Q8K4J6
Protein Ontology More...PROⁱ	PR:Q8K4J6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000042292 Expressed in 277 organ(s), highest expression level in dentate gyrus granule cell layer
CleanExⁱ	MM_MKL1
ExpressionAtlasⁱ	Q8K4J6 baseline and differential
Genevisibleⁱ	Q8K4J6 MM

Family and domain databases

Gene3Dⁱ	1.10.720.30, 1 hit
InterProⁱ	View protein in InterPro IPR029992 MRTF-A IPR004018 RPEL_repeat IPR003034 SAP_dom IPR036361 SAP_dom_sf
PANTHERⁱ	PTHR22793:SF6 PTHR22793:SF6, 2 hits
Pfamⁱ	View protein in Pfam PF02755 RPEL, 3 hits PF02037 SAP, 1 hit
SMARTⁱ	View protein in SMART SM00707 RPEL, 3 hits SM00513 SAP, 1 hit
SUPFAMⁱ	SSF68906 SSF68906, 1 hit
PROSITEⁱ	View protein in PROSITE PS51073 RPEL, 3 hits PS50800 SAP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MRTFA_MOUSE
Accessionⁱ	Q8K4J6Primary (citable) accession number: Q8K4J6 Secondary accession number(s): Q642U1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
	Last sequence update:	June 27, 2003
	Last modified:	November 7, 2018
	This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - Q8K4J6 (MRTFA_MOUSE)

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Myocardin-related transcription factor A

Mrtfa

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

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Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ