UniProtKB - Q8K4I4 (BPIA1_RAT)
Protein
BPI fold-containing family A member 1
Gene
Bpifa1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Lipid-binding protein which shows high specificity for the surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC). Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils.By similarity
Caution
Reported to bind to bacterial lipopolysaccharide (LPS) in vitro. However, the in vivo significance of this is uncertain since other studies indicate little or no specificity for LPS.By similarity
GO - Molecular functioni
- lipid binding Source: UniProtKB-KW
GO - Biological processi
- antibacterial humoral response Source: UniProtKB
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: RGD
- defense response to virus Source: RGD
- immune response in nasopharyngeal-associated lymphoid tissue Source: RGD
- innate immune response Source: UniProtKB
- multicellular organismal water homeostasis Source: UniProtKB
- negative regulation of single-species biofilm formation in or on host organism Source: UniProtKB
- regulation of liquid surface tension Source: UniProtKB
- regulation of sodium ion transmembrane transport Source: UniProtKB
Keywordsi
Molecular function | Antibiotic, Antimicrobial |
Biological process | Immunity, Innate immunity |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-RNO-6803157, Antimicrobial peptides |
Names & Taxonomyi
Protein namesi | Recommended name: BPI fold-containing family A member 1Alternative name(s): Palate lung and nasal epithelium clone protein |
Gene namesi | Name:Bpifa1 Synonyms:Plunc |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619818, Bpifa1 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Note: Apical side of airway epithelial cells. Detected in airway surface liquid, nasal mucus and sputum.
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: UniProtKB
Other locations
- microvillus Source: RGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000017178 | 20 – 270 | BPI fold-containing family A member 1Add BLAST | 251 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 174 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 196 ↔ 238 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q8K4I4 |
PTM databases
GlyGeni | Q8K4I4, 1 site |
Expressioni
Tissue specificityi
Detected in adult nasal epithelium, heart, lung, spleen, testis and salivary gland, and in embryonic nasal epithelium, lung, salivary gland and thymus.1 Publication
Inductioni
After bulbectomy or lesion of the olfactory bulb.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000013859, Expressed in testis and 8 other tissues |
Genevisiblei | Q8K4I4, RN |
Interactioni
Subunit structurei
Monomer.
Interacts (via N-terminus) with SCNN1B, a subunit of the heterotrimeric epithelial sodium channel (ENaC); this inhibits proteolytic activation of ENaC (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000018581 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 104 – 109 | Important for surfactant activity and antibacterial propertiesBy similarity | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 20 – 156 | Gly/Leu/Pro-richAdd BLAST | 137 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SR58, Eukaryota |
GeneTreei | ENSGT00970000193386 |
HOGENOMi | CLU_095915_0_0_1 |
InParanoidi | Q8K4I4 |
OMAi | LAVRDKQ |
OrthoDBi | 1275829at2759 |
PhylomeDBi | Q8K4I4 |
TreeFami | TF337052 |
Family and domain databases
InterProi | View protein in InterPro IPR017943, Bactericidal_perm-incr_a/b_dom IPR034307, BPIFA1 IPR017942, Lipid-bd_serum_glycop_N |
PANTHERi | PTHR47015:SF1, PTHR47015:SF1, 1 hit |
Pfami | View protein in Pfam PF01273, LBP_BPI_CETP, 1 hit |
SUPFAMi | SSF55394, SSF55394, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8K4I4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFLVGSLVVL CGLLAQSTAQ LAGLPLPLGQ GLPLPLGQGL PLPLGQGLPL
60 70 80 90 100
AVSPALPSNP TDLLAGNFAN ALSGGLLSGG LLGILENIPL LDVIKSGGGS
110 120 130 140 150
SNGLVGGLLG KLTSSVPLLN NILDIKITDP RLLELGLVQS PDGHRLYATI
160 170 180 190 200
PLSLKLQVNM PVVGSFLQLA VKLNITAEIV AMKDNQGRIH LVLGDCTHSP
210 220 230 240 250
GSLQITLLNG VTPVQSSLDS LTGILTKVLP ELIQGKVCPL INGILSGLDV
260 270
TLVHNIAELL IHGIQFVIKV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF393750 mRNA Translation: AAM73687.1 |
RefSeqi | NP_742028.1, NM_172031.1 |
Genome annotation databases
Ensembli | ENSRNOT00000018581; ENSRNOP00000018581; ENSRNOG00000013859 |
GeneIDi | 246238 |
KEGGi | rno:246238 |
UCSCi | RGD:619818, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF393750 mRNA Translation: AAM73687.1 |
RefSeqi | NP_742028.1, NM_172031.1 |
3D structure databases
SMRi | Q8K4I4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000018581 |
PTM databases
GlyGeni | Q8K4I4, 1 site |
Proteomic databases
PaxDbi | Q8K4I4 |
Genome annotation databases
Ensembli | ENSRNOT00000018581; ENSRNOP00000018581; ENSRNOG00000013859 |
GeneIDi | 246238 |
KEGGi | rno:246238 |
UCSCi | RGD:619818, rat |
Organism-specific databases
CTDi | 51297 |
RGDi | 619818, Bpifa1 |
Phylogenomic databases
eggNOGi | ENOG502SR58, Eukaryota |
GeneTreei | ENSGT00970000193386 |
HOGENOMi | CLU_095915_0_0_1 |
InParanoidi | Q8K4I4 |
OMAi | LAVRDKQ |
OrthoDBi | 1275829at2759 |
PhylomeDBi | Q8K4I4 |
TreeFami | TF337052 |
Enzyme and pathway databases
Reactomei | R-RNO-6803157, Antimicrobial peptides |
Miscellaneous databases
PROi | PR:Q8K4I4 |
Gene expression databases
Bgeei | ENSRNOG00000013859, Expressed in testis and 8 other tissues |
Genevisiblei | Q8K4I4, RN |
Family and domain databases
InterProi | View protein in InterPro IPR017943, Bactericidal_perm-incr_a/b_dom IPR034307, BPIFA1 IPR017942, Lipid-bd_serum_glycop_N |
PANTHERi | PTHR47015:SF1, PTHR47015:SF1, 1 hit |
Pfami | View protein in Pfam PF01273, LBP_BPI_CETP, 1 hit |
SUPFAMi | SSF55394, SSF55394, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BPIA1_RAT | |
Accessioni | Q8K4I4Primary (citable) accession number: Q8K4I4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families