UniProtKB - Q8K4H1 (KFA_MOUSE)
Protein
Kynurenine formamidase
Gene
Afmid
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation1 Publication
Miscellaneous
The Afmid gene shares a bidirectional promoter region with Tk1 gene.
Catalytic activityi
- EC:3.5.1.9UniRule annotation
Kineticsi
- KM=0.18 mM for N-formyl-L-kynurenine1 Publication
- Vmax=42 µmol/min/mg enzyme1 Publication
: L-tryptophan degradation via kynurenine pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Tryptophan 2,3-dioxygenase (Tdo2), Tryptophan 2,3-dioxygenase (Tdo2), Tryptophan 2,3-dioxygenase (Tdo2), Indoleamine 2,3-dioxygenase 2 (Ido2)
- Kynurenine formamidase (Afmid)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 162 | NucleophileCurated | 1 | |
Active sitei | 247 | Curated | 1 | |
Active sitei | 279 | Curated | 1 |
GO - Molecular functioni
- arylformamidase activity Source: GO_Central
- hydrolase activity Source: GO_Central
GO - Biological processi
- 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-UniRule
- NAD biosynthetic process Source: GO_Central
- tryptophan catabolic process to kynurenine Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Tryptophan catabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-15003 |
BRENDAi | 3.5.1.9, 3474 |
SABIO-RKi | Q8K4H1 |
UniPathwayi | UPA00333;UER00454 |
Protein family/group databases
ESTHERi | mouse-KFA, Kynurenine-formamidase |
MEROPSi | S09.977 |
Names & Taxonomyi
Protein namesi | Recommended name: Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)Short name: KFAUniRule annotation Short name: KFaseUniRule annotation Alternative name(s): ArylformamidaseUniRule annotation N-formylkynurenine formamidaseUniRule annotation Short name: FKFUniRule annotation |
Gene namesi | Name:Afmid |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2448704, Afmid |
Pathology & Biotechi
Disruption phenotypei
Mice display sclerosis of kidney glomeruli, possibly due to failures in the elimination of toxic metabolites.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 162 | S → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 247 | D → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 279 | H → A: Loss of enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248309 | 1 – 305 | Kynurenine formamidaseAdd BLAST | 305 |
Post-translational modificationi
The N-terminus is blocked.
Proteomic databases
jPOSTi | Q8K4H1 |
PaxDbi | Q8K4H1 |
PRIDEi | Q8K4H1 |
PTM databases
iPTMneti | Q8K4H1 |
PhosphoSitePlusi | Q8K4H1 |
Expressioni
Tissue specificityi
Highly expressed in liver. Expressed in kidney. Weakly or not expressed in other tissues.1 Publication
Inductioni
Down-regulated upon IL2-mediated activation. Transcriptional activation correlates with reduced histone acetylation.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000017718, Expressed in cortex of kidney and 120 other tissues |
ExpressionAtlasi | Q8K4H1, baseline and differential |
Genevisiblei | Q8K4H1, MM |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
IntActi | Q8K4H1, 2 interactors |
MINTi | Q8K4H1 |
STRINGi | 10090.ENSMUSP00000073102 |
Miscellaneous databases
RNActi | Q8K4H1, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 93 – 97 | HGGXW | 5 |
Domaini
The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation
Sequence similaritiesi
Belongs to the kynurenine formamidase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG4627, Eukaryota |
GeneTreei | ENSGT00390000011093 |
HOGENOMi | CLU_012494_4_7_1 |
InParanoidi | Q8K4H1 |
OMAi | WSHRMAA |
OrthoDBi | 992858at2759 |
PhylomeDBi | Q8K4H1 |
TreeFami | TF315112 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
HAMAPi | MF_03014, KFase, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR013094, AB_hydrolase_3 IPR027519, KFase_ver/fungi-typ |
Pfami | View protein in Pfam PF07859, Abhydrolase_3, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8K4H1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAFPSLSAGQ NPWRNLSSEE LEKQYSPSRW VIHTKPEEVV GNFVQIGSQA
60 70 80 90 100
TQKARATRRN QLDVPYGDGE GEKLDIYFPD EDSKAFPLFL FLHGGYWQSG
110 120 130 140 150
SKDDSAFMVN PLTAQGIVVV IVAYDIAPKG TLDQMVDQVT RSVVFLQRRY
160 170 180 190 200
PSNEGIYLCG HSAGAHLAAM VLLARWTKHG VTPNLQGFLL VSGIYDLEPL
210 220 230 240 250
IATSQNDPLR MTLEDAQRNS PQRHLDVVPA QPVAPACPVL VLVGQHDSPE
260 270 280 290 300
FHRQSKEFYE TLLRVGWKAS FQQLRGVDHF DIIENLTRED DVLTQIILKT
VFQKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB1AQL0 | B1AQL0_MOUSE | Kynurenine formamidase | Afmid | 363 | Annotation score: |
Sequence cautioni
The sequence AAH24452 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | H → R in AAH43309 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 33 | H → R in AAH24452 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020237 | 296 – 305 | IILKTVFQKL → VGLTPTTVFLNGQRGSHAHT WAWPGRQEQRERLAVPRWKS QSRLHRRACGECLLFIIVVA ETMSHCITLAGLGLKI in isoform 2. 1 Publication | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF399717 mRNA Translation: AAM62284.1 AY099479 mRNA Translation: AAM44406.1 AL591433 Genomic DNA No translation available. BC024452 mRNA Translation: AAH24452.1 Different initiation. BC043309 mRNA Translation: AAH43309.1 BC066780 mRNA Translation: AAH66780.1 |
CCDSi | CCDS25693.1 [Q8K4H1-1] |
RefSeqi | NP_082103.1, NM_027827.3 [Q8K4H1-1] XP_006534309.1, XM_006534246.1 |
Genome annotation databases
Ensembli | ENSMUST00000073388; ENSMUSP00000073102; ENSMUSG00000017718 [Q8K4H1-1] |
GeneIDi | 71562 |
KEGGi | mmu:71562 |
UCSCi | uc007moa.2, mouse [Q8K4H1-2] uc007mob.2, mouse [Q8K4H1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF399717 mRNA Translation: AAM62284.1 AY099479 mRNA Translation: AAM44406.1 AL591433 Genomic DNA No translation available. BC024452 mRNA Translation: AAH24452.1 Different initiation. BC043309 mRNA Translation: AAH43309.1 BC066780 mRNA Translation: AAH66780.1 |
CCDSi | CCDS25693.1 [Q8K4H1-1] |
RefSeqi | NP_082103.1, NM_027827.3 [Q8K4H1-1] XP_006534309.1, XM_006534246.1 |
3D structure databases
SMRi | Q8K4H1 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8K4H1, 2 interactors |
MINTi | Q8K4H1 |
STRINGi | 10090.ENSMUSP00000073102 |
Protein family/group databases
ESTHERi | mouse-KFA, Kynurenine-formamidase |
MEROPSi | S09.977 |
PTM databases
iPTMneti | Q8K4H1 |
PhosphoSitePlusi | Q8K4H1 |
Proteomic databases
jPOSTi | Q8K4H1 |
PaxDbi | Q8K4H1 |
PRIDEi | Q8K4H1 |
Protocols and materials databases
Antibodypediai | 19735, 78 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000073388; ENSMUSP00000073102; ENSMUSG00000017718 [Q8K4H1-1] |
GeneIDi | 71562 |
KEGGi | mmu:71562 |
UCSCi | uc007moa.2, mouse [Q8K4H1-2] uc007mob.2, mouse [Q8K4H1-1] |
Organism-specific databases
CTDi | 125061 |
MGIi | MGI:2448704, Afmid |
Phylogenomic databases
eggNOGi | KOG4627, Eukaryota |
GeneTreei | ENSGT00390000011093 |
HOGENOMi | CLU_012494_4_7_1 |
InParanoidi | Q8K4H1 |
OMAi | WSHRMAA |
OrthoDBi | 992858at2759 |
PhylomeDBi | Q8K4H1 |
TreeFami | TF315112 |
Enzyme and pathway databases
UniPathwayi | UPA00333;UER00454 |
BioCyci | MetaCyc:MONOMER-15003 |
BRENDAi | 3.5.1.9, 3474 |
SABIO-RKi | Q8K4H1 |
Miscellaneous databases
BioGRID-ORCSi | 71562, 2 hits in 17 CRISPR screens |
ChiTaRSi | Afmid, mouse |
PROi | PR:Q8K4H1 |
RNActi | Q8K4H1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000017718, Expressed in cortex of kidney and 120 other tissues |
ExpressionAtlasi | Q8K4H1, baseline and differential |
Genevisiblei | Q8K4H1, MM |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
HAMAPi | MF_03014, KFase, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR013094, AB_hydrolase_3 IPR027519, KFase_ver/fungi-typ |
Pfami | View protein in Pfam PF07859, Abhydrolase_3, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KFA_MOUSE | |
Accessioni | Q8K4H1Primary (citable) accession number: Q8K4H1 Secondary accession number(s): B1AQK9, Q80XP3, Q8R1K6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families