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Protein

Probable 28S rRNA (cytosine-C(5))-methyltransferase

Gene

Nsun5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of a cytosine in 28S rRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine in 28S rRNA = S-adenosyl-L-homocysteine + C5-methylcytosine in 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei258S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei263S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei305S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei359NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 28S rRNA (cytosine-C(5))-methyltransferase (EC:2.1.1.-)
Alternative name(s):
NOL1/NOP2/Sun domain family member 5
Williams-Beuren syndrome chromosomal region 20A protein homolog
Gene namesi
Name:Nsun5
Synonyms:Wbscr20, Wbscr20a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140844 Nsun5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002616702 – 465Probable 28S rRNA (cytosine-C(5))-methyltransferaseAdd BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei167PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8K4F6
MaxQBiQ8K4F6
PaxDbiQ8K4F6
PeptideAtlasiQ8K4F6
PRIDEiQ8K4F6

PTM databases

iPTMnetiQ8K4F6
PhosphoSitePlusiQ8K4F6

Expressioni

Gene expression databases

BgeeiENSMUSG00000000916 Expressed in 234 organ(s), highest expression level in entire extraembryonic component
CleanExiMM_NSUN5
ExpressionAtlasiQ8K4F6 baseline and differential
GenevisibleiQ8K4F6 MM

Interactioni

Protein-protein interaction databases

BioGridi221497, 1 interactor
IntActiQ8K4F6, 1 interactor
MINTiQ8K4F6
STRINGi10090.ENSMUSP00000000940

Structurei

3D structure databases

ProteinModelPortaliQ8K4F6
SMRiQ8K4F6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni234 – 240S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2360 Eukaryota
COG0144 LUCA
GeneTreeiENSGT00550000074709
HOGENOMiHOG000203203
HOVERGENiHBG062278
InParanoidiQ8K4F6
KOiK15264
OMAiLVCETQR
OrthoDBiEOG091G0TW9
PhylomeDBiQ8K4F6
TreeFamiTF314285

Family and domain databases

InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8K4F6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLYAAAAAV LAGVESRQGS LKGLVYSSNF QNLKQLYALV CETQRYSAVL
60 70 80 90 100
DAVIASAGLL RAEKKLRPHL AKVLVYELLL GKGFRGGGGR WKALLGRHQA
110 120 130 140 150
RLKAELARLK VHRGVSRNED LLQESSRPGQ AYQVPRFVRV NTLKTRPEDA
160 170 180 190 200
IDYFKRQGFS YQGRASSLED LRALKGQHFL LDPLLPELLV FPAQTDLHEH
210 220 230 240 250
PLYRAGHLIL QDKASCLPAM LLSPPPGSHV IDACAAPGNK TSYIAALLKN
260 270 280 290 300
QGKIFAFDQD AKRLAAMATL VARAGVSCCE LAEKDFLTVS PSDQRYSQVQ
310 320 330 340 350
YILLDPSCSG SGMLSRQLEE HGEGTPSKER LQALAGFQQR ALCHALRFPS
360 370 380 390 400
LQRLVYSTCS LCQEENEDVV QEALQHNSGT FRLAPVLPTW PHRGLSTFPG
410 420 430 440 450
SEHCLRASPE TTLTGGFFIA VFERAEVVPT PAPQTDAMDP EPLSQVPKRK
460
RRRKAAVGAS MQPST
Length:465
Mass (Da):51,030
Last modified:November 28, 2006 - v2
Checksum:iE0545B5290D46D9A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QAY1E9QAY1_MOUSE
Probable 28S rRNA (cytosine-C(5))-m...
Nsun5
91Annotation score:
A0A0J9YTU6A0A0J9YTU6_MOUSE
Probable 28S rRNA (cytosine-C(5))-m...
Nsun5
142Annotation score:

Sequence cautioni

The sequence AAH51209 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40V → M in BAE33793 (PubMed:16141072).Curated1
Sequence conflicti130Q → H in AAM62311 (PubMed:12073013).Curated1
Sequence conflicti396S → N in BAE33793 (PubMed:16141072).Curated1
Sequence conflicti443L → F in AAH51209 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF412029 mRNA Translation: AAM62311.1
AK079400 mRNA Translation: BAC37634.1
AK149521 mRNA Translation: BAE28935.1
AK156651 mRNA Translation: BAE33793.1
BC051209 mRNA Translation: AAH51209.1 Different initiation.
CCDSiCCDS19740.1
RefSeqiNP_663389.2, NM_145414.2
UniGeneiMm.281189

Genome annotation databases

EnsembliENSMUST00000000940; ENSMUSP00000000940; ENSMUSG00000000916
GeneIDi100609
KEGGimmu:100609
UCSCiuc008zyh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF412029 mRNA Translation: AAM62311.1
AK079400 mRNA Translation: BAC37634.1
AK149521 mRNA Translation: BAE28935.1
AK156651 mRNA Translation: BAE33793.1
BC051209 mRNA Translation: AAH51209.1 Different initiation.
CCDSiCCDS19740.1
RefSeqiNP_663389.2, NM_145414.2
UniGeneiMm.281189

3D structure databases

ProteinModelPortaliQ8K4F6
SMRiQ8K4F6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221497, 1 interactor
IntActiQ8K4F6, 1 interactor
MINTiQ8K4F6
STRINGi10090.ENSMUSP00000000940

PTM databases

iPTMnetiQ8K4F6
PhosphoSitePlusiQ8K4F6

Proteomic databases

EPDiQ8K4F6
MaxQBiQ8K4F6
PaxDbiQ8K4F6
PeptideAtlasiQ8K4F6
PRIDEiQ8K4F6

Protocols and materials databases

DNASUi100609
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000940; ENSMUSP00000000940; ENSMUSG00000000916
GeneIDi100609
KEGGimmu:100609
UCSCiuc008zyh.1 mouse

Organism-specific databases

CTDi55695
MGIiMGI:2140844 Nsun5

Phylogenomic databases

eggNOGiKOG2360 Eukaryota
COG0144 LUCA
GeneTreeiENSGT00550000074709
HOGENOMiHOG000203203
HOVERGENiHBG062278
InParanoidiQ8K4F6
KOiK15264
OMAiLVCETQR
OrthoDBiEOG091G0TW9
PhylomeDBiQ8K4F6
TreeFamiTF314285

Miscellaneous databases

ChiTaRSiNsun5 mouse
PROiPR:Q8K4F6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000916 Expressed in 234 organ(s), highest expression level in entire extraembryonic component
CleanExiMM_NSUN5
ExpressionAtlasiQ8K4F6 baseline and differential
GenevisibleiQ8K4F6 MM

Family and domain databases

InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNSUN5_MOUSE
AccessioniPrimary (citable) accession number: Q8K4F6
Secondary accession number(s): Q3U0Q8, Q80WG3, Q8C568
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: September 12, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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