UniProtKB - Q8K4F6 (NSUN5_MOUSE)
Protein
28S rRNA (cytosine-C(5))-methyltransferase
Gene
Nsun5
Organism
Mus musculus (Mouse)
Status
Functioni
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 3438 (m5C3438) in 28S rRNA (PubMed:31722427). m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity (By similarity). Required for corpus callosum and cerebral cortex development (PubMed:31174389, PubMed:31462248).By similarity3 Publications
Catalytic activityi
- a cytidine in 28S rRNA + S-adenosyl-L-methionine = a 5-methylcytidine in 28S rRNA + H+ + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 258 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 263 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 305 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Active sitei | 359 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- rRNA (cytosine-C5-)-methyltransferase activity Source: UniProtKB
GO - Biological processi
- cerebral cortex development Source: UniProtKB
- cognition Source: UniProtKB
- corpus callosum development Source: UniProtKB
- oligodendrocyte development Source: UniProtKB
- positive regulation of translation Source: UniProtKB
- regulation of myelination Source: UniProtKB
- RNA methylation Source: GO_Central
- rRNA base methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: 28S rRNA (cytosine-C(5))-methyltransferaseCurated (EC:2.1.1.-1 Publication)Alternative name(s): NOL1/NOP2/Sun domain family member 52 Publications Williams-Beuren syndrome chromosomal region 20A protein homolog1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2140844, Nsun5 |
Subcellular locationi
Nucleus
- nucleolus By similarity
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Decreased body weight and lean mass without alterations in food intake (PubMed:31722427). Adult mice show spatial cognitive deficits, possibly caused by defects in development and function of oligodendrocyte precursor cells (PubMed:30485550). Mice display a reduction of the corpus callosum with a decline in the number of myelinated axons and loose myelin sheath (PubMed:31174389). They also show impaired development of the cerebral cortex, characterized by impaired growth of radial glial scaffold (PubMed:31462248).4 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000261670 | 2 – 465 | 28S rRNA (cytosine-C(5))-methyltransferaseAdd BLAST | 464 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylglycineBy similarity | 1 | |
Modified residuei | 167 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8K4F6 |
MaxQBi | Q8K4F6 |
PaxDbi | Q8K4F6 |
PeptideAtlasi | Q8K4F6 |
PRIDEi | Q8K4F6 |
PTM databases
iPTMneti | Q8K4F6 |
PhosphoSitePlusi | Q8K4F6 |
Expressioni
Tissue specificityi
In the hippocampus, specifically expressed in adult hippocampal NG2-positive oligodendrocyte precursor cells (at protein level).1 Publication
Developmental stagei
Present in the developing cerebral cortex from embryonic day (E) 12.5, with a peak at E14.5 followed by a decrease from E18.5 (at protein level) (PubMed:31462248). Selectively expressed in radial glial cells of cerebral cortex from E12.5 to E16.5, but not in intermediate progenitor cells (IPCs) or neocortical neurons (at protein level) (PubMed:31462248). Highly expressed in callosal oligodendrocyte precursor cells (OPCs) and oligodendrocytes (OLs) from postnatal day 7 to postnatal day 28 (PubMed:31174389).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000000916, Expressed in entire extraembryonic component and 252 other tissues |
Genevisiblei | Q8K4F6, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 221497, 1 interactor |
IntActi | Q8K4F6, 1 interactor |
MINTi | Q8K4F6 |
STRINGi | 10090.ENSMUSP00000000940 |
Miscellaneous databases
RNActi | Q8K4F6, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 234 – 240 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 7 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG2360, Eukaryota |
GeneTreei | ENSGT00940000155974 |
HOGENOMi | CLU_005316_7_4_1 |
InParanoidi | Q8K4F6 |
OMAi | GKGLKCG |
OrthoDBi | 1128973at2759 |
PhylomeDBi | Q8K4F6 |
TreeFami | TF314285 |
Family and domain databases
InterProi | View protein in InterPro IPR001678, MeTrfase_RsmB/NOP2 IPR023267, RCMT IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit |
PRINTSi | PR02008, RCMTFAMILY |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51686, SAM_MT_RSMB_NOP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8K4F6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLYAAAAAV LAGVESRQGS LKGLVYSSNF QNLKQLYALV CETQRYSAVL
60 70 80 90 100
DAVIASAGLL RAEKKLRPHL AKVLVYELLL GKGFRGGGGR WKALLGRHQA
110 120 130 140 150
RLKAELARLK VHRGVSRNED LLQESSRPGQ AYQVPRFVRV NTLKTRPEDA
160 170 180 190 200
IDYFKRQGFS YQGRASSLED LRALKGQHFL LDPLLPELLV FPAQTDLHEH
210 220 230 240 250
PLYRAGHLIL QDKASCLPAM LLSPPPGSHV IDACAAPGNK TSYIAALLKN
260 270 280 290 300
QGKIFAFDQD AKRLAAMATL VARAGVSCCE LAEKDFLTVS PSDQRYSQVQ
310 320 330 340 350
YILLDPSCSG SGMLSRQLEE HGEGTPSKER LQALAGFQQR ALCHALRFPS
360 370 380 390 400
LQRLVYSTCS LCQEENEDVV QEALQHNSGT FRLAPVLPTW PHRGLSTFPG
410 420 430 440 450
SEHCLRASPE TTLTGGFFIA VFERAEVVPT PAPQTDAMDP EPLSQVPKRK
460
RRRKAAVGAS MQPST
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YTU6 | A0A0J9YTU6_MOUSE | 28S rRNA (cytosine-C(5))-methyltran... | Nsun5 | 142 | Annotation score: | ||
E9QAY1 | E9QAY1_MOUSE | 28S rRNA (cytosine-C(5))-methyltran... | Nsun5 | 91 | Annotation score: |
Sequence cautioni
The sequence AAH51209 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | V → M in BAE33793 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 130 | Q → H in AAM62311 (PubMed:12073013).Curated | 1 | |
Sequence conflicti | 396 | S → N in BAE33793 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 443 | L → F in AAH51209 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF412029 mRNA Translation: AAM62311.1 AK079400 mRNA Translation: BAC37634.1 AK149521 mRNA Translation: BAE28935.1 AK156651 mRNA Translation: BAE33793.1 BC051209 mRNA Translation: AAH51209.1 Different initiation. |
CCDSi | CCDS19740.1 |
RefSeqi | NP_663389.2, NM_145414.2 |
Genome annotation databases
Ensembli | ENSMUST00000000940; ENSMUSP00000000940; ENSMUSG00000000916 |
GeneIDi | 100609 |
KEGGi | mmu:100609 |
UCSCi | uc008zyh.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF412029 mRNA Translation: AAM62311.1 AK079400 mRNA Translation: BAC37634.1 AK149521 mRNA Translation: BAE28935.1 AK156651 mRNA Translation: BAE33793.1 BC051209 mRNA Translation: AAH51209.1 Different initiation. |
CCDSi | CCDS19740.1 |
RefSeqi | NP_663389.2, NM_145414.2 |
3D structure databases
SMRi | Q8K4F6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221497, 1 interactor |
IntActi | Q8K4F6, 1 interactor |
MINTi | Q8K4F6 |
STRINGi | 10090.ENSMUSP00000000940 |
PTM databases
iPTMneti | Q8K4F6 |
PhosphoSitePlusi | Q8K4F6 |
Proteomic databases
EPDi | Q8K4F6 |
MaxQBi | Q8K4F6 |
PaxDbi | Q8K4F6 |
PeptideAtlasi | Q8K4F6 |
PRIDEi | Q8K4F6 |
Protocols and materials databases
Antibodypediai | 14275, 80 antibodies |
DNASUi | 100609 |
Genome annotation databases
Ensembli | ENSMUST00000000940; ENSMUSP00000000940; ENSMUSG00000000916 |
GeneIDi | 100609 |
KEGGi | mmu:100609 |
UCSCi | uc008zyh.1, mouse |
Organism-specific databases
CTDi | 55695 |
MGIi | MGI:2140844, Nsun5 |
Phylogenomic databases
eggNOGi | KOG2360, Eukaryota |
GeneTreei | ENSGT00940000155974 |
HOGENOMi | CLU_005316_7_4_1 |
InParanoidi | Q8K4F6 |
OMAi | GKGLKCG |
OrthoDBi | 1128973at2759 |
PhylomeDBi | Q8K4F6 |
TreeFami | TF314285 |
Miscellaneous databases
BioGRID-ORCSi | 100609, 4 hits in 18 CRISPR screens |
ChiTaRSi | Nsun5, mouse |
PROi | PR:Q8K4F6 |
RNActi | Q8K4F6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000916, Expressed in entire extraembryonic component and 252 other tissues |
Genevisiblei | Q8K4F6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001678, MeTrfase_RsmB/NOP2 IPR023267, RCMT IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit |
PRINTSi | PR02008, RCMTFAMILY |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51686, SAM_MT_RSMB_NOP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NSUN5_MOUSE | |
Accessioni | Q8K4F6Primary (citable) accession number: Q8K4F6 Secondary accession number(s): Q3U0Q8, Q80WG3, Q8C568 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | November 28, 2006 | |
Last modified: | February 10, 2021 | |
This is version 120 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families