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UniProtKB - Q8K4D6 (CP4X1_RAT)
Protein
Cytochrome P450 4X1
Gene
Cyp4x1
Organism
Rattus norvegicus (Rat)
Status
Functioni
A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
By similarityCatalytic activityi
- N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + O2 + reduced [NADPH—hemoprotein reductase] = H+ + H2O + N-(14,15-epoxy-5Z,8Z,11Z-eicosatrienoyl)-ethanolamine + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 452 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- anandamide 14,15 epoxidase activity Source: UniProtKB
- heme binding Source: InterPro
- iron ion binding Source: InterPro
GO - Biological processi
- lipid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 4X11 Publication (EC:1.14.14.-By similarity)Alternative name(s): CYPIVX1 |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628719, Cyp4x1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
- Microsome membrane By similarity; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051863 | 1 – 507 | Cytochrome P450 4X1Add BLAST | 507 |
Proteomic databases
PaxDbi | Q8K4D6 |
Expressioni
Tissue specificityi
Expressed at high levels in brain, mainly in neurons in different regions, including brain stem, hippocampus, cortex and cerebellum. Also expressed in cerebral vasculature. Not detected in kidney, nor liver.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000043513, Expressed in frontal cortex and 12 other tissues |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000160927 |
HOGENOMi | CLU_001570_5_1_1 |
InParanoidi | Q8K4D6 |
OMAi | SDQRHPY |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q8K4D6 |
TreeFami | TF105088 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8K4D6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEASWLENRW ARPLHLALVF CLALVLMQAV KLYLRRQRLL RDLRPFPGPT
60 70 80 90 100
AHWLLGHQKF LQEDNMEKLD EIVKEYPCAF PCWVGPFQAF FYIYDPDYAK
110 120 130 140 150
IFLSRTDPKT QYLHQLMTPF LGRGLLNLDG PRWFQHRCLL TPAFHQDILK
160 170 180 190 200
PCVDMMAHSV NMMLDKWEKT WTTQETTIEV FEHINLMTLD IIMKCAFGQE
210 220 230 240 250
TNCQINGTYE SYVKATFELG EIISSRLYNF WHHHDIIFKL SPKGHCFQEL
260 270 280 290 300
GKVIHQCTEK IIQDRKKTLK DQVNQDDTQT SQNFLDIVLS AQAGDEKAFS
310 320 330 340 350
DADLRSEVNT FMWAGHDASA ASISWLLYCL ALNPEHQDRC RTEIRSILGD
360 370 380 390 400
GSSITWEQLD EIPYTTMCIK ETLRLIPPIP SISRELSKPL TLPDGHSLPA
410 420 430 440 450
GMTVVLSIWG LHHNPAVWKD PKVFDPLRFT KENSEQRHPC AFLPFSSGPR
460 470 480 490 500
NCIGQQFAML ELKVAIALTL LRFRVAADLT RPPAFSSHTV LRPKHGIYLH
LKKLPEC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF439343 mRNA Translation: AAM73782.1 |
PIRi | JC7883 |
RefSeqi | NP_663708.1, NM_145675.1 |
Genome annotation databases
Ensembli | ENSRNOT00000011985; ENSRNOP00000011985; ENSRNOG00000043513 |
GeneIDi | 246767 |
KEGGi | rno:246767 |
UCSCi | RGD:628719, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF439343 mRNA Translation: AAM73782.1 |
PIRi | JC7883 |
RefSeqi | NP_663708.1, NM_145675.1 |
3D structure databases
SMRi | Q8K4D6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000011985 |
Proteomic databases
PaxDbi | Q8K4D6 |
Genome annotation databases
Ensembli | ENSRNOT00000011985; ENSRNOP00000011985; ENSRNOG00000043513 |
GeneIDi | 246767 |
KEGGi | rno:246767 |
UCSCi | RGD:628719, rat |
Organism-specific databases
CTDi | 260293 |
RGDi | 628719, Cyp4x1 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000160927 |
HOGENOMi | CLU_001570_5_1_1 |
InParanoidi | Q8K4D6 |
OMAi | SDQRHPY |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q8K4D6 |
TreeFami | TF105088 |
Miscellaneous databases
PROi | PR:Q8K4D6 |
Gene expression databases
Bgeei | ENSRNOG00000043513, Expressed in frontal cortex and 12 other tissues |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4X1_RAT | |
Accessioni | Q8K4D6Primary (citable) accession number: Q8K4D6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families