UniProtKB - Q8K4B2 (IRAK3_MOUSE)
Protein
Interleukin-1 receptor-associated kinase 3
Gene
Irak3
Organism
Mus musculus (Mouse)
Status
Functioni
Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.1 Publication2 Publications
Caution
Asn-306 is present instead of the conserved Asp which is expected to be an active site residue. Low level autophosphorylation activity has been reported in PubMed:12054681, while other authors describe this as an inactive kinase.Curated
Catalytic activityi
Cofactori
Mg2+1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 205 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 324 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 184 – 192 | ATPPROSITE-ProRule annotationBy similarity | 9 | |
Nucleotide bindingi | 308 – 311 | ATPPROSITE-ProRule annotation | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- protein kinase activity Source: MGI
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- cytokine-mediated signaling pathway Source: UniProtKB
- interleukin-1-mediated signaling pathway Source: MGI
- intracellular signal transduction Source: GO_Central
- MyD88-dependent toll-like receptor signaling pathway Source: InterPro
- negative regulation of cytokine-mediated signaling pathway Source: BHF-UCL
- negative regulation of innate immune response Source: BHF-UCL
- negative regulation of interleukin-12 production Source: BHF-UCL
- negative regulation of interleukin-6 production Source: BHF-UCL
- negative regulation of macrophage cytokine production Source: BHF-UCL
- negative regulation of MAP kinase activity Source: BHF-UCL
- negative regulation of NF-kappaB transcription factor activity Source: MGI
- negative regulation of protein catabolic process Source: BHF-UCL
- negative regulation of protein-containing complex disassembly Source: MGI
- negative regulation of toll-like receptor signaling pathway Source: BHF-UCL
- negative regulation of tumor necrosis factor production Source: BHF-UCL
- positive regulation of macrophage tolerance induction Source: BHF-UCL
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- protein autophosphorylation Source: MGI
- protein phosphorylation Source: UniProtKB
- regulation of protein-containing complex disassembly Source: UniProtKB
- response to exogenous dsRNA Source: BHF-UCL
- response to interleukin-1 Source: MGI
- response to lipopolysaccharide Source: BHF-UCL
- response to peptidoglycan Source: BHF-UCL
- response to virus Source: BHF-UCL
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin-1 receptor-associated kinase 3 (EC:2.7.11.1)Short name: IRAK-3 Alternative name(s): IL-1 receptor-associated kinase M Short name: IRAK-M |
Gene namesi | Name:Irak3Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921164, Irak3 |
Subcellular locationi
Nucleus
- nucleus Source: BHF-UCL
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: BHF-UCL
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086034 | 1 – 609 | Interleukin-1 receptor-associated kinase 3Add BLAST | 609 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 480 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8K4B2 |
MaxQBi | Q8K4B2 |
PaxDbi | Q8K4B2 |
PRIDEi | Q8K4B2 |
ProteomicsDBi | 267005 |
PTM databases
iPTMneti | Q8K4B2 |
PhosphoSitePlusi | Q8K4B2 |
Expressioni
Tissue specificityi
Highly expressed in liver and thymus and at lower levels in heart, brain, spleen and kidney.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000020227, Expressed in granulocyte and 126 other tissues |
Genevisiblei | Q8K4B2, MM |
Interactioni
Binary interactionsi
Hide detailsQ8K4B2
With | #Exp. | IntAct |
---|---|---|
Irak4 [Q8R4K2] | 7 | EBI-646179,EBI-3842721 |
Traf6 [P70196] | 3 | EBI-646179,EBI-448028 |
IRAK4 [Q9NWZ3] from Homo sapiens. | 4 | EBI-646179,EBI-448378 |
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 216351, 3 interactors |
IntActi | Q8K4B2, 19 interactors |
MINTi | Q8K4B2 |
STRINGi | 10090.ENSMUSP00000020448 |
Miscellaneous databases
RNActi | Q8K4B2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 106 | DeathPROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 178 – 463 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 286 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1187, Eukaryota |
GeneTreei | ENSGT00940000161222 |
HOGENOMi | CLU_029530_0_0_1 |
InParanoidi | Q8K4B2 |
OrthoDBi | 684563at2759 |
PhylomeDBi | Q8K4B2 |
TreeFami | TF328924 |
Family and domain databases
CDDi | cd08796, Death_IRAK-M, 1 hit cd14160, PK_IRAK3, 1 hit |
InterProi | View protein in InterPro IPR011029, DEATH-like_dom_sf IPR000488, Death_domain IPR033610, IRAK3 IPR042747, IRAK3_death IPR042698, IRAK3_PK IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom |
PANTHERi | PTHR24419:SF7, PTHR24419:SF7, 1 hit |
Pfami | View protein in Pfam PF00531, Death, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00005, DEATH, 1 hit |
SUPFAMi | SSF47986, SSF47986, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50017, DEATH_DOMAIN, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8K4B2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGRCGARGA LSPQLLLFDL PPALLGELCG ILDSCDGPLG WRGLAERLSN
60 70 80 90 100
SWLDVRHIEK YVNQGKSGTR ELLWSWAQKN KTIGDLLEVL QDMGHQRAIH
110 120 130 140 150
LIINYGVSWT PSVQTHHELP FPSFPPEVKH ACRENDPGPL EPANVTVDNV
160 170 180 190 200
LVPEHNEKGT LQKTPISFQS ILEGTKHFHK DFLIGEGEIF EVYRVDIRNQ
210 220 230 240 250
AYAVKLFKQE KKMQLKKHWK RFLSELEVLL LFRHPHILEL AAYFTETEKL
260 270 280 290 300
CLVYPYMSNG TLFDRLQCTN GTTPLSWHVR ISVLIGIAKA IQYLHNTQPC
310 320 330 340 350
AVICGNVSSA NILLDDQLQP KLTDFAAAHF RPNLEQQSST INMTGGGRKH
360 370 380 390 400
LWYMPEEYIR QGRLSVKTDV YSFGIVIMEV LTGCKVVLDD PKHVQLRDLL
410 420 430 440 450
MELMEKRGLD SCLSFLDRKI PPCPRNFSAK LFSLAGRCVA TKAKLRPTMD
460 470 480 490 500
EVLSSLESTQ PSLYFAEDPP TSLKSFRCPS PLFLDNVPSI PVEDDENQNN
510 520 530 540 550
HSVPPKEVLG TDRVTQKTPF ECSQSEVTFL GLDRNRGNRG SEADCNVPSS
560 570 580 590 600
SHEECWSPEL VAPSQDLSPT VISLGSSWEV PGHSYGSKPM EKRCSSGLFC
SEHEQSKKQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RCU1 | D6RCU1_MOUSE | Interleukin-1 receptor-associated k... | Irak3 | 309 | Annotation score: | ||
D3Z007 | D3Z007_MOUSE | Interleukin-1 receptor-associated k... | Irak3 | 517 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | C → W in AAM83393 (PubMed:12150927).Curated | 1 | |
Sequence conflicti | 42 | R → W in AAM83393 (PubMed:12150927).Curated | 1 | |
Sequence conflicti | 62 | V → L in AAM83393 (PubMed:12150927).Curated | 1 | |
Sequence conflicti | 126 | P → L in AAM83393 (PubMed:12150927).Curated | 1 | |
Sequence conflicti | 147 | V → M in AAM83393 (PubMed:12150927).Curated | 1 | |
Sequence conflicti | 282 | S → G in BAC33612 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 282 | S → N in AAM83393 (PubMed:12150927).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF461763 mRNA Translation: AAM83393.1 AJ440757 mRNA Translation: CAD29448.2 AK029057 mRNA Translation: BAC26270.1 AK049210 mRNA Translation: BAC33612.1 |
CCDSi | CCDS24204.1 |
RefSeqi | NP_082955.2, NM_028679.3 |
Genome annotation databases
Ensembli | ENSMUST00000020448; ENSMUSP00000020448; ENSMUSG00000020227 |
GeneIDi | 73914 |
KEGGi | mmu:73914 |
UCSCi | uc007het.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF461763 mRNA Translation: AAM83393.1 AJ440757 mRNA Translation: CAD29448.2 AK029057 mRNA Translation: BAC26270.1 AK049210 mRNA Translation: BAC33612.1 |
CCDSi | CCDS24204.1 |
RefSeqi | NP_082955.2, NM_028679.3 |
3D structure databases
SMRi | Q8K4B2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216351, 3 interactors |
IntActi | Q8K4B2, 19 interactors |
MINTi | Q8K4B2 |
STRINGi | 10090.ENSMUSP00000020448 |
PTM databases
iPTMneti | Q8K4B2 |
PhosphoSitePlusi | Q8K4B2 |
Proteomic databases
jPOSTi | Q8K4B2 |
MaxQBi | Q8K4B2 |
PaxDbi | Q8K4B2 |
PRIDEi | Q8K4B2 |
ProteomicsDBi | 267005 |
Protocols and materials databases
Antibodypediai | 16652, 562 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020448; ENSMUSP00000020448; ENSMUSG00000020227 |
GeneIDi | 73914 |
KEGGi | mmu:73914 |
UCSCi | uc007het.1, mouse |
Organism-specific databases
CTDi | 11213 |
MGIi | MGI:1921164, Irak3 |
Phylogenomic databases
eggNOGi | KOG1187, Eukaryota |
GeneTreei | ENSGT00940000161222 |
HOGENOMi | CLU_029530_0_0_1 |
InParanoidi | Q8K4B2 |
OrthoDBi | 684563at2759 |
PhylomeDBi | Q8K4B2 |
TreeFami | TF328924 |
Miscellaneous databases
BioGRID-ORCSi | 73914, 1 hit in 52 CRISPR screens |
ChiTaRSi | Irak3, mouse |
PROi | PR:Q8K4B2 |
RNActi | Q8K4B2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020227, Expressed in granulocyte and 126 other tissues |
Genevisiblei | Q8K4B2, MM |
Family and domain databases
CDDi | cd08796, Death_IRAK-M, 1 hit cd14160, PK_IRAK3, 1 hit |
InterProi | View protein in InterPro IPR011029, DEATH-like_dom_sf IPR000488, Death_domain IPR033610, IRAK3 IPR042747, IRAK3_death IPR042698, IRAK3_PK IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom |
PANTHERi | PTHR24419:SF7, PTHR24419:SF7, 1 hit |
Pfami | View protein in Pfam PF00531, Death, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00005, DEATH, 1 hit |
SUPFAMi | SSF47986, SSF47986, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50017, DEATH_DOMAIN, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IRAK3_MOUSE | |
Accessioni | Q8K4B2Primary (citable) accession number: Q8K4B2 Secondary accession number(s): Q8C7U8, Q8CE40, Q8K1S8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 9, 2004 |
Last sequence update: | October 25, 2004 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families