UniProtKB - Q8K448 (ABCA5_MOUSE)
Protein
ATP-binding cassette sub-family A member 5
Gene
Abca5
Organism
Mus musculus (Mouse)
Status
Functioni
May play a role in the processing of autolysosomes.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 514 – 521 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1333 – 1340 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- lipid transporter activity Source: GO_Central
GO - Biological processi
- cholesterol efflux Source: BHF-UCL
- high-density lipoprotein particle remodeling Source: BHF-UCL
- lipid transport Source: GO_Central
- negative regulation of macrophage derived foam cell differentiation Source: BHF-UCL
- reverse cholesterol transport Source: BHF-UCL
Keywordsi
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1369062, ABC transporters in lipid homeostasis |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-binding cassette sub-family A member 5 |
Gene namesi | Name:Abca5 Synonyms:Kiaa1888 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2386607, Abca5 |
Subcellular locationi
Lysosome
- Lysosome membrane 1 Publication; Multi-pass membrane protein 1 Publication
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity
Endosome
- Late endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endosome
- late endosome Source: BHF-UCL
- late endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: BHF-UCL
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 32 – 52 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 220 – 240 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 264 – 284 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 297 – 317 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 328 – 348 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 355 – 375 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 396 – 416 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 864 – 884 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 967 – 987 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1021 – 1041 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1071 – 1091 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1102 – 1122 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1138 – 1158 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1164 – 1184 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1207 – 1227 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endosome, Golgi apparatus, Lysosome, MembranePathology & Biotechi
Disruption phenotypei
Mice display hypothyroidism and lethal heart abnormalities that may be due to altered autolysosomes processing.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000250670 | 1 – 1642 | ATP-binding cassette sub-family A member 5Add BLAST | 1642 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 86 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 388 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 458 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 919 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 996 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q8K448 |
PaxDbi | Q8K448 |
PeptideAtlasi | Q8K448 |
PRIDEi | Q8K448 |
PTM databases
GlyGeni | Q8K448, 5 sites |
iPTMneti | Q8K448 |
PhosphoSitePlusi | Q8K448 |
Expressioni
Tissue specificityi
Expressed in cardiomyocytes, oligodendrocytes and astrocytes in brain, alveolar type 2 cells in lung and follicular cells in the thyroid gland (at protein level). Detected in brain, testis, lung, heart, liver, kidney, skeletal muscle and placenta.2 Publications
Developmental stagei
Expressed during embryogenesis.1 Publication
Inductioni
Down-regulated by digoxin.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000018800, Expressed in utricle of membranous labyrinth and 224 other tissues |
ExpressionAtlasi | Q8K448, baseline and differential |
Genevisiblei | Q8K448, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 229877, 4 interactors |
STRINGi | 10090.ENSMUSP00000047927 |
Miscellaneous databases
RNActi | Q8K448, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 478 – 713 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 236 | |
Domaini | 1290 – 1533 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 244 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0059, Eukaryota |
GeneTreei | ENSGT00940000158172 |
HOGENOMi | CLU_000604_19_1_1 |
InParanoidi | Q8K448 |
OMAi | RIRTWTK |
OrthoDBi | 131191at2759 |
PhylomeDBi | Q8K448 |
TreeFami | TF105192 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR026082, ABCA IPR030367, ABCA5 IPR027417, P-loop_NTPase |
PANTHERi | PTHR19229, PTHR19229, 1 hit PTHR19229:SF100, PTHR19229:SF100, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8K448-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATAIRDVGV WRQTRTLLLK NYLIKCRTKK SSVQEILFPL FFLFWLILVS
60 70 80 90 100
MMHPNKKYEE VSDIELSPMD KFSLSNVILG YTPVTNITSS IMQRVSTDHL
110 120 130 140 150
PKVIVTEEYA NEKELVAASL SKSSNFVGVV FKDTMSYELR FFPEMIPVSS
160 170 180 190 200
IYMNSREGCS KTCDAAQYWS LGFTVLQASI DAAIIQLKTN VSVWSELEST
210 220 230 240 250
KAVIMGEAAV VEIDTFPRGV ILIYLVIAFS PFGYFLAIHI VAEKEKKLKE
260 270 280 290 300
FLKIMGLHDT AFWLSWVLLY ASLIFLMSLL MAVIATASSL FPQSSSIVIF
310 320 330 340 350
LLFFLYGLSS VFFALMLTPL FKKSKHVGVV EFFVTVVFGF VGLLIVLIES
360 370 380 390 400
FPRSLVWLFS PLCQCAFLIG IAQVMHLEDF NEGALFSNLT EGPYPLIITI
410 420 430 440 450
IMLALDSVFY VLLAVYLDQV IPGEFGLRRS SLYFLKPSYW SKNKRNYKEL
460 470 480 490 500
SEGNINGNIS LNEIVEPVSS EFIGKEAIRI SGIQKSYRKK TENVEALRNL
510 520 530 540 550
SFDIYEGQIT ALLGHSGTGK STLMNILCGL CPPSDGFASI YGHRVSEIDE
560 570 580 590 600
MFEARKMIGI CPQSDINFDV LTVEENLSIL ASIKGIPANN IIQEVQKVLL
610 620 630 640 650
DLDMQAIKDN QAKKLSGGQK RKLSVGIAVL GNPKILLLDE PTAGMDPCSR
660 670 680 690 700
HIVWNLLKYR KANRVTVFST HFMDEADILA DRKAVISQGM LKCVGSSIFL
710 720 730 740 750
KSKWGIGYRL SMYIDRYCAT ESLSSLVRQH IPAAALLQQN DQQLVYSLPF
760 770 780 790 800
KDMDKFSGLF SALDIHSNLG VISYGVSMTT LEDVFLKLEV EAEIDQADYS
810 820 830 840 850
VFTQQPREEE TDSKSFDEME QSLLILSETK ASSVSTMSLW KQQVSTIAKF
860 870 880 890 900
HFLSLKRESK SVRAVLLLLL IFFAVQIFMF FLHHSFKNAV VPIKLVPDLY
910 920 930 940 950
FLKPGDKPHK YKTSLLLQNS TDSDINGLIE FFAHQNIMVA MFNDSDYVSA
960 970 980 990 1000
APHSAALNVV RSEKDYVFSA VFNSTMVYCL PVMMNIISNY YLYHLNVTEA
1010 1020 1030 1040 1050
IQTWSTPFIQ EITDIVFKIE LYFQAALLGI IVTAMPPYFA MENAENHKIK
1060 1070 1080 1090 1100
AYTQLKLSGL LPSAYWVGQA VVDIPLFFVV LILMLGSLFA FHHGLYFYPA
1110 1120 1130 1140 1150
KFLAVVFCLI AYVPSVILFT YIASFTFKKI LNTKEFWSFI YSVTALACVA
1160 1170 1180 1190 1200
ITETTFFLQY AVTAVFHYTF CIAIPIYPLL GCLISFIKGS WKNMPKNENT
1210 1220 1230 1240 1250
YNPWDRLLVA VIMPYLQCIL WIFLLQHYEK IHGGRSIRKD PFFRALSQKA
1260 1270 1280 1290 1300
KNKKFPEPPI NEDEDEDVKA ERLKVKELMG CQCCEEKPAI MVCNLHKEYD
1310 1320 1330 1340 1350
DKKDFLHSRK TTKVATKYIS FCVKKGEILG LLGPNGAGKS TVINTLVGDV
1360 1370 1380 1390 1400
EPTSGKIFLG DYGSHSSEDD ESIKCMGYCP QTNPLWPDLT LQEHFEIYGA
1410 1420 1430 1440 1450
VKGMSPGDMK EVISRITKAL DLKEHLQKTV KKLPAGIKRK LCFALSMLGN
1460 1470 1480 1490 1500
PQVTLLDEPS TGMDPRAKQH MWRAIRTAFK NKKRAALLTT HYMEEAEAVC
1510 1520 1530 1540 1550
DRVAIMVSGQ LRCIGTVQHL KSKFGKGYFL EIKLKDWIEN LEIDRLQREI
1560 1570 1580 1590 1600
QYIFPNASRQ ESFSSILAFK IPKEDVQSLS QSFAKLEEAK RTFAIEEYSF
1610 1620 1630 1640
SQATLEQVFV ELTKEQEEED NSCGTLASTL WWERTQEDRV VF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AEP5 | A2AEP5_MOUSE | ATP-binding cassette sub-family A m... | Abca5 | 1,194 | Annotation score: | ||
A2AEP6 | A2AEP6_MOUSE | ATP-binding cassette sub-family A m... | Abca5 | 181 | Annotation score: |
Sequence cautioni
The sequence BAC28896 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | S → C in BAC32984 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 113 | K → E in BAE41431 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 132 | K → E in BAC32984 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 188 | K → E in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 303 | F → V in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 384 | A → P in BAC32984 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 530 | L → P in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 551 | M → I in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 809 | E → G in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 839 | L → P in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 856 | K → N in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 880 | F → V in AAM90895 (PubMed:12532264).Curated | 1 | |
Sequence conflicti | 1231 | I → K in AAH64823 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1503 | V → G in AAM90895 (PubMed:12532264).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF491842 mRNA Translation: AAM90895.1 AB097675 mRNA Translation: BAC66658.1 AK034961 mRNA Translation: BAC28896.2 Different initiation. AK047188 mRNA Translation: BAC32984.1 AK138604 mRNA Translation: BAE23713.1 AK169879 mRNA Translation: BAE41431.1 BC064823 mRNA Translation: AAH64823.1 Sequence problems. AK129463 mRNA Translation: BAC98273.1 |
CCDSi | CCDS25591.1 |
RefSeqi | NP_671752.2, NM_147219.2 XP_006533128.1, XM_006533065.3 |
Genome annotation databases
Ensembli | ENSMUST00000043961; ENSMUSP00000047927; ENSMUSG00000018800 |
GeneIDi | 217265 |
KEGGi | mmu:217265 |
UCSCi | uc007mdm.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF491842 mRNA Translation: AAM90895.1 AB097675 mRNA Translation: BAC66658.1 AK034961 mRNA Translation: BAC28896.2 Different initiation. AK047188 mRNA Translation: BAC32984.1 AK138604 mRNA Translation: BAE23713.1 AK169879 mRNA Translation: BAE41431.1 BC064823 mRNA Translation: AAH64823.1 Sequence problems. AK129463 mRNA Translation: BAC98273.1 |
CCDSi | CCDS25591.1 |
RefSeqi | NP_671752.2, NM_147219.2 XP_006533128.1, XM_006533065.3 |
3D structure databases
SMRi | Q8K448 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229877, 4 interactors |
STRINGi | 10090.ENSMUSP00000047927 |
PTM databases
GlyGeni | Q8K448, 5 sites |
iPTMneti | Q8K448 |
PhosphoSitePlusi | Q8K448 |
Proteomic databases
MaxQBi | Q8K448 |
PaxDbi | Q8K448 |
PeptideAtlasi | Q8K448 |
PRIDEi | Q8K448 |
Protocols and materials databases
Antibodypediai | 19335, 172 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043961; ENSMUSP00000047927; ENSMUSG00000018800 |
GeneIDi | 217265 |
KEGGi | mmu:217265 |
UCSCi | uc007mdm.1, mouse |
Organism-specific databases
CTDi | 23461 |
MGIi | MGI:2386607, Abca5 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0059, Eukaryota |
GeneTreei | ENSGT00940000158172 |
HOGENOMi | CLU_000604_19_1_1 |
InParanoidi | Q8K448 |
OMAi | RIRTWTK |
OrthoDBi | 131191at2759 |
PhylomeDBi | Q8K448 |
TreeFami | TF105192 |
Enzyme and pathway databases
Reactomei | R-MMU-1369062, ABC transporters in lipid homeostasis |
Miscellaneous databases
BioGRID-ORCSi | 217265, 2 hits in 17 CRISPR screens |
ChiTaRSi | Abca5, mouse |
PROi | PR:Q8K448 |
RNActi | Q8K448, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000018800, Expressed in utricle of membranous labyrinth and 224 other tissues |
ExpressionAtlasi | Q8K448, baseline and differential |
Genevisiblei | Q8K448, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR026082, ABCA IPR030367, ABCA5 IPR027417, P-loop_NTPase |
PANTHERi | PTHR19229, PTHR19229, 1 hit PTHR19229:SF100, PTHR19229:SF100, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCA5_MOUSE | |
Accessioni | Q8K448Primary (citable) accession number: Q8K448 Secondary accession number(s): Q3TE17 Q8BXG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | October 3, 2006 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families