UniProtKB - Q8K409 (DPOLB_MOUSE)
Protein
DNA polymerase beta
Gene
Polb
Organism
Mus musculus (Mouse)
Status
Functioni
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity).By similarity
Catalytic activityi
- EC:2.7.7.7
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 72 | Schiff-base intermediate with DNABy similarity | 1 | |
Metal bindingi | 101 | Sodium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 103 | Sodium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 106 | Sodium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 190 | Magnesium 1By similarity | 1 | |
Metal bindingi | 190 | Magnesium 2By similarity | 1 | |
Metal bindingi | 192 | Magnesium 1By similarity | 1 | |
Metal bindingi | 192 | Magnesium 2By similarity | 1 | |
Metal bindingi | 256 | Magnesium 2By similarity | 1 |
GO - Molecular functioni
- damaged DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-directed DNA polymerase activity Source: UniProtKB
- enzyme binding Source: MGI
- lyase activity Source: UniProtKB
- metal ion binding Source: MGI
- microtubule binding Source: MGI
GO - Biological processi
- aging Source: Ensembl
- apoptotic process Source: MGI
- base-excision repair Source: UniProtKB
- base-excision repair, gap-filling Source: MGI
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA replication Source: UniProtKB-KW
- double-strand break repair via nonhomologous end joining Source: GO_Central
- homeostasis of number of cells Source: MGI
- immunoglobulin heavy chain V-D-J recombination Source: MGI
- inflammatory response Source: MGI
- intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- lymph node development Source: MGI
- neuron apoptotic process Source: MGI
- pyrimidine dimer repair Source: MGI
- response to ethanol Source: Ensembl
- response to gamma radiation Source: Ensembl
- response to hyperoxia Source: Ensembl
- salivary gland morphogenesis Source: MGI
- somatic diversification of immunoglobulins Source: MGI
- somatic hypermutation of immunoglobulin genes Source: MGI
- spleen development Source: MGI
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication, DNA synthesis |
Ligand | Magnesium, Metal-binding, Sodium |
Enzyme and pathway databases
Reactomei | R-MMU-110362, POLB-Dependent Long Patch Base Excision Repair R-MMU-110373, Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-MMU-110381, Resolution of AP sites via the single-nucleotide replacement pathway R-MMU-5649702, APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway R-MMU-5651801, PCNA-Dependent Long Patch Base Excision Repair R-MMU-5689880, Ub-specific processing proteases R-MMU-73930, Abasic sugar-phosphate removal via the single-nucleotide replacement pathway |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Polb |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97740, Polb |
Subcellular locationi
Cytoskeleton
- microtubule Source: MGI
- spindle microtubule Source: MGI
Nucleus
- nucleus Source: MGI
- synaptonemal complex Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218779 | 1 – 335 | DNA polymerase betaAdd BLAST | 335 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 41 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 61 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 72 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 81 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 83 | Omega-N-methylarginine; by PRMT6By similarity | 1 | |
Modified residuei | 152 | Omega-N-methylarginine; by PRMT6By similarity | 1 |
Post-translational modificationi
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.By similarity
Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Ubl conjugationProteomic databases
EPDi | Q8K409 |
MaxQBi | Q8K409 |
PaxDbi | Q8K409 |
PeptideAtlasi | Q8K409 |
PRIDEi | Q8K409 |
PTM databases
iPTMneti | Q8K409 |
PhosphoSitePlusi | Q8K409 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031536, Expressed in spermatid and 314 other tissues |
ExpressionAtlasi | Q8K409, baseline and differential |
Genevisiblei | Q8K409, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47 (By similarity).
Interacts with FAM168A (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
- microtubule binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202289, 8 interactors |
IntActi | Q8K409, 2 interactors |
MINTi | Q8K409 |
STRINGi | 10090.ENSMUSP00000033938 |
Chemistry databases
BindingDBi | Q8K409 |
Miscellaneous databases
RNActi | Q8K409, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 183 – 192 | DNA bindingBy similarity | 10 |
Domaini
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.By similarity
Sequence similaritiesi
Belongs to the DNA polymerase type-X family.Curated
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000156918 |
HOGENOMi | CLU_008698_1_0_1 |
InParanoidi | Q8K409 |
OMAi | ITDMLME |
OrthoDBi | 1328151at2759 |
PhylomeDBi | Q8K409 |
TreeFami | TF103002 |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR002008, DNA_pol_X_beta-like IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR043519, NT_sf IPR029398, PolB_thumb |
Pfami | View protein in Pfam PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00870, DNAPOLXBETA |
SMARTi | View protein in SMART SM00278, HhH1, 2 hits SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS00522, DNA_POLYMERASE_X, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8K409-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKRKAPQET LNGGITDMLV ELANFEKNVS QAIHKYNAYR KAASVIAKYP
60 70 80 90 100
HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL
110 120 130 140 150
TRVTGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFEDFEKRI
160 170 180 190 200
PREEMLQMQD IVLNEIKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPNF
210 220 230 240 250
TSESSKQPKL LHRVVEQLQK VHFITDTLSK GETKFMGVCQ LPSEKDGKEY
260 270 280 290 300
PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP
310 320 330
LGVTGVAGEP LPVDSEQDIF DYIQWRYREP KDRSE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GT62 | A0A1B0GT62_MOUSE | DNA polymerase beta | Polb | 111 | Annotation score: |
Sequence cautioni
The sequence AAH06681 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF513911 mRNA Translation: AAM49616.1 AK077127 mRNA Translation: BAC36630.1 AK146745 mRNA Translation: BAE27405.1 AK151436 mRNA Translation: BAE30399.1 BC006681 mRNA Translation: AAH06681.1 Sequence problems. BC060998 mRNA Translation: AAH60998.1 |
CCDSi | CCDS22181.1 |
RefSeqi | NP_035260.1, NM_011130.2 |
Genome annotation databases
Ensembli | ENSMUST00000033938; ENSMUSP00000033938; ENSMUSG00000031536 |
GeneIDi | 18970 |
KEGGi | mmu:18970 |
UCSCi | uc009ldk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF513911 mRNA Translation: AAM49616.1 AK077127 mRNA Translation: BAC36630.1 AK146745 mRNA Translation: BAE27405.1 AK151436 mRNA Translation: BAE30399.1 BC006681 mRNA Translation: AAH06681.1 Sequence problems. BC060998 mRNA Translation: AAH60998.1 |
CCDSi | CCDS22181.1 |
RefSeqi | NP_035260.1, NM_011130.2 |
3D structure databases
BMRBi | Q8K409 |
SMRi | Q8K409 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202289, 8 interactors |
IntActi | Q8K409, 2 interactors |
MINTi | Q8K409 |
STRINGi | 10090.ENSMUSP00000033938 |
Chemistry databases
BindingDBi | Q8K409 |
ChEMBLi | CHEMBL4565 |
PTM databases
iPTMneti | Q8K409 |
PhosphoSitePlusi | Q8K409 |
Proteomic databases
EPDi | Q8K409 |
MaxQBi | Q8K409 |
PaxDbi | Q8K409 |
PeptideAtlasi | Q8K409 |
PRIDEi | Q8K409 |
Protocols and materials databases
Antibodypediai | 3175, 388 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033938; ENSMUSP00000033938; ENSMUSG00000031536 |
GeneIDi | 18970 |
KEGGi | mmu:18970 |
UCSCi | uc009ldk.2, mouse |
Organism-specific databases
CTDi | 5423 |
MGIi | MGI:97740, Polb |
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000156918 |
HOGENOMi | CLU_008698_1_0_1 |
InParanoidi | Q8K409 |
OMAi | ITDMLME |
OrthoDBi | 1328151at2759 |
PhylomeDBi | Q8K409 |
TreeFami | TF103002 |
Enzyme and pathway databases
Reactomei | R-MMU-110362, POLB-Dependent Long Patch Base Excision Repair R-MMU-110373, Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-MMU-110381, Resolution of AP sites via the single-nucleotide replacement pathway R-MMU-5649702, APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway R-MMU-5651801, PCNA-Dependent Long Patch Base Excision Repair R-MMU-5689880, Ub-specific processing proteases R-MMU-73930, Abasic sugar-phosphate removal via the single-nucleotide replacement pathway |
Miscellaneous databases
BioGRID-ORCSi | 18970, 0 hits in 17 CRISPR screens |
ChiTaRSi | Polb, mouse |
PROi | PR:Q8K409 |
RNActi | Q8K409, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031536, Expressed in spermatid and 314 other tissues |
ExpressionAtlasi | Q8K409, baseline and differential |
Genevisiblei | Q8K409, MM |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR002008, DNA_pol_X_beta-like IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR043519, NT_sf IPR029398, PolB_thumb |
Pfami | View protein in Pfam PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00870, DNAPOLXBETA |
SMARTi | View protein in SMART SM00278, HhH1, 2 hits SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS00522, DNA_POLYMERASE_X, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOLB_MOUSE | |
Accessioni | Q8K409Primary (citable) accession number: Q8K409 Secondary accession number(s): Q3UAB6, Q922Z7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families