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Protein

Protein CASC3

Gene

Casc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Stress response, Translation regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72187 mRNA 3'-end processing
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CASC3
Alternative name(s):
Cancer susceptibility candidate gene 3 protein homolog
Metastatic lymph node gene 51 protein homolog
Short name:
MLN 51 homolog
Protein barentsz1 Publication
Short name:
Btz1 Publication
Short name:
mBtz1 Publication
Gene namesi
Name:Casc3
Synonyms:Mln51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2179723 Casc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi464L → A: Accumulation in the nucleus. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000893251 – 698Protein CASC3Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei115PhosphoserineBy similarity1
Modified residuei145PhosphoserineCombined sources1
Modified residuei262PhosphoserineBy similarity1
Modified residuei263PhosphoserineCombined sources1
Modified residuei354PhosphothreonineBy similarity1
Modified residuei360PhosphoserineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei472PhosphoserineBy similarity1

Post-translational modificationi

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination (By similarity).By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8K3W3
MaxQBiQ8K3W3
PaxDbiQ8K3W3
PeptideAtlasiQ8K3W3
PRIDEiQ8K3W3

PTM databases

iPTMnetiQ8K3W3
PhosphoSitePlusiQ8K3W3

Expressioni

Tissue specificityi

High levels in heart, brain, including hippocampus and cerebellum, liver, kidney and testis; lower levels in muscle, lung and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000078676 Expressed in 266 organ(s), highest expression level in cumulus cell
CleanExiMM_CASC3
GenevisibleiQ8K3W3 MM

Interactioni

Subunit structurei

Component of the mRNA splicing-dependent exon junction complex (EJC), which contains at least CASC3, EIF4A3, MAGOH, NXF1 and RBM8A/Y14 (By similarity). Forms homooligomers (By similarity). Interacts with STAU in an RNA-dependent manner (PubMed:12843282).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228651, 3 interactors
ComplexPortaliCPX-635 Exon junction core complex, Magoh variant
CPX-683 Exon junction core complex, Magohb variant
IntActiQ8K3W3, 1 interactor
STRINGi10090.ENSMUSP00000017384

Structurei

3D structure databases

ProteinModelPortaliQ8K3W3
SMRiQ8K3W3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 280Necessary for RNA-binding, interaction with MAGOH and localization in nucleus specklesBy similarityAdd BLAST147
Regioni134 – 280Sufficient to form the EJCBy similarityAdd BLAST147
Regioni374 – 698Necessary for localization in cytoplasmic stress granulesBy similarityAdd BLAST325

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili98 – 127Sequence analysisAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 207Nuclear localization signal 1Sequence analysis7
Motifi251 – 259Nuclear localization signal 2Sequence analysis9
Motifi457 – 466Nuclear export signal10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 45Poly-Gly6
Compositional biasi638 – 642Poly-Pro5
Compositional biasi686 – 689Poly-Pro4

Domaini

The coiled coil domain may be involved in oligomerization.By similarity

Sequence similaritiesi

Belongs to the CASC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4264 Eukaryota
ENOG410ZW5Y LUCA
GeneTreeiENSGT00390000006930
HOGENOMiHOG000069997
HOVERGENiHBG050799
InParanoidiQ8K3W3
KOiK14323
OMAiPAKTGNW
OrthoDBiEOG091G0JLP
PhylomeDBiQ8K3W3
TreeFamiTF329663

Family and domain databases

InterProiView protein in InterPro
IPR018545 Btz_dom
IPR028544 CASC3
PANTHERiPTHR13434 PTHR13434, 1 hit
PfamiView protein in Pfam
PF09405 Btz, 1 hit
SMARTiView protein in SMART
SM01044 Btz, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K3W3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADRRRQRAS QDTEDEESGA SGSDSGSPAR GGGSCSGSVG GGGSGSLPSQ
60 70 80 90 100
RGGRGGGLHL RRVESGGAKS AEESECESED GMEGDAVLSD YESAEDSEGE
110 120 130 140 150
EDYSEEENSK VELKSEANDA ADSSAKEKGE EKPESKGTVT GERQSGDGQE
160 170 180 190 200
STEPVENKVG KKGPKHLDDD EDRKNPAYIP RKGLFFEHDL RGQTQEEEVR
210 220 230 240 250
PKGRQRKLWK DEGRWEHDKF REDEQAPKSR QELIALYGYD IRSAHNPDDI
260 270 280 290 300
KPRRIRKPRF GSSPQRDPNW IGDRSSKSHR HQGPGGNLPP RTFINRNTAG
310 320 330 340 350
TGRMSASRNY SRSGGFKDGR TSFRPVEVAG QHGGRSAETL KHEASYRSRR
360 370 380 390 400
LEQTPVRDPS PEPDAPLLGS PEKEEVASET PAAVPDITPP APDRPIEKKS
410 420 430 440 450
YSRARRTRTK VGDAVKAAEE VPPPSEGLAS TATVPETTPA AKTGNWEAPV
460 470 480 490 500
DSTTGGLEQD VAQLNIAEQS WSPSQPSFLQ PRELRGVPNH IHMGAGPPPQ
510 520 530 540 550
FNRMEEMGVQ SGRAKRYSSQ RQRPVPEPPA PPVHISIMEG HYYDPLQFQG
560 570 580 590 600
PIYTHGDSPA PLPPQGMIVQ PEMHLPHPGL HPHQSPGPLP NPGLYPPPVS
610 620 630 640 650
MSPGQPPPQQ LLAPTYFSAP GVMNFGNPNY PYAPGALPPP PPPHLYPNTQ
660 670 680 690
APPQVYGGVT YYNPAQQQVQ PKPSPPRRTP QPVSIKPPPP EVVSRGSS
Length:698
Mass (Da):75,770
Last modified:July 7, 2009 - v3
Checksum:i3B718FC297A5E0F3
GO

Sequence cautioni

The sequence AAH60672 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297N → D in BAE24081 (PubMed:16141072).Curated1
Sequence conflicti474S → G in AAH34533 (PubMed:15489334).Curated1
Sequence conflicti589L → V in BAE24081 (PubMed:16141072).Curated1
Sequence conflicti638Missing in CAC27775 (PubMed:12843282).Curated1
Sequence conflicti638Missing in AAH60672 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF526276 mRNA Translation: AAM88396.1
AJ292072 mRNA Translation: CAC27775.1
AK139608 mRNA Translation: BAE24081.1
AL590963 Genomic DNA Translation: CAM46181.1
CH466556 Genomic DNA Translation: EDL16170.1
BC034533 mRNA Translation: AAH34533.1
BC060672 mRNA Translation: AAH60672.1 Different initiation.
BC141296 mRNA Translation: AAI41297.1
CCDSiCCDS36302.1
RefSeqiNP_619601.2, NM_138660.2
UniGeneiMm.40120

Genome annotation databases

EnsembliENSMUST00000017384; ENSMUSP00000017384; ENSMUSG00000078676
ENSMUST00000169695; ENSMUSP00000130926; ENSMUSG00000078676
GeneIDi192160
KEGGimmu:192160
UCSCiuc007lhq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF526276 mRNA Translation: AAM88396.1
AJ292072 mRNA Translation: CAC27775.1
AK139608 mRNA Translation: BAE24081.1
AL590963 Genomic DNA Translation: CAM46181.1
CH466556 Genomic DNA Translation: EDL16170.1
BC034533 mRNA Translation: AAH34533.1
BC060672 mRNA Translation: AAH60672.1 Different initiation.
BC141296 mRNA Translation: AAI41297.1
CCDSiCCDS36302.1
RefSeqiNP_619601.2, NM_138660.2
UniGeneiMm.40120

3D structure databases

ProteinModelPortaliQ8K3W3
SMRiQ8K3W3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228651, 3 interactors
ComplexPortaliCPX-635 Exon junction core complex, Magoh variant
CPX-683 Exon junction core complex, Magohb variant
IntActiQ8K3W3, 1 interactor
STRINGi10090.ENSMUSP00000017384

PTM databases

iPTMnetiQ8K3W3
PhosphoSitePlusiQ8K3W3

Proteomic databases

EPDiQ8K3W3
MaxQBiQ8K3W3
PaxDbiQ8K3W3
PeptideAtlasiQ8K3W3
PRIDEiQ8K3W3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017384; ENSMUSP00000017384; ENSMUSG00000078676
ENSMUST00000169695; ENSMUSP00000130926; ENSMUSG00000078676
GeneIDi192160
KEGGimmu:192160
UCSCiuc007lhq.1 mouse

Organism-specific databases

CTDi22794
MGIiMGI:2179723 Casc3

Phylogenomic databases

eggNOGiKOG4264 Eukaryota
ENOG410ZW5Y LUCA
GeneTreeiENSGT00390000006930
HOGENOMiHOG000069997
HOVERGENiHBG050799
InParanoidiQ8K3W3
KOiK14323
OMAiPAKTGNW
OrthoDBiEOG091G0JLP
PhylomeDBiQ8K3W3
TreeFamiTF329663

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72187 mRNA 3'-end processing
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

ChiTaRSiCasc3 mouse
PROiPR:Q8K3W3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078676 Expressed in 266 organ(s), highest expression level in cumulus cell
CleanExiMM_CASC3
GenevisibleiQ8K3W3 MM

Family and domain databases

InterProiView protein in InterPro
IPR018545 Btz_dom
IPR028544 CASC3
PANTHERiPTHR13434 PTHR13434, 1 hit
PfamiView protein in Pfam
PF09405 Btz, 1 hit
SMARTiView protein in SMART
SM01044 Btz, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCASC3_MOUSE
AccessioniPrimary (citable) accession number: Q8K3W3
Secondary accession number(s): A3KFP7
, Q3UT99, Q8K219, Q99NF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: July 7, 2009
Last modified: November 7, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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