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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

Ndufs8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi119Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi123Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105 Respiratory electron transport
R-MMU-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
Name:Ndufs8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385079 Ndufs8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34MitochondrionBy similarityAdd BLAST34
ChainiPRO_000002001435 – 212NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialAdd BLAST178

Proteomic databases

EPDiQ8K3J1
MaxQBiQ8K3J1
PaxDbiQ8K3J1
PRIDEiQ8K3J1

2D gel databases

REPRODUCTION-2DPAGEiQ8K3J1

PTM databases

iPTMnetiQ8K3J1
PhosphoSitePlusiQ8K3J1
SwissPalmiQ8K3J1

Expressioni

Gene expression databases

BgeeiENSMUSG00000059734 Expressed in 271 organ(s), highest expression level in brown adipose tissue
CleanExiMM_NDUFS8
ExpressionAtlasiQ8K3J1 baseline and differential
GenevisibleiQ8K3J1 MM

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

BioGridi230440, 1 interactor
ComplexPortaliCPX-266 Mitochondrial respiratory chain complex I
CORUMiQ8K3J1
IntActiQ8K3J1, 4 interactors
MINTiQ8K3J1
STRINGi10090.ENSMUSP00000074600

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8K3J1
SMRiQ8K3J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 1334Fe-4S ferredoxin-type 1Add BLAST30
Domaini143 – 1724Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256 Eukaryota
COG1143 LUCA
GeneTreeiENSGT00390000003049
HOGENOMiHOG000228289
HOVERGENiHBG006547
InParanoidiQ8K3J1
KOiK03941
OMAiLCGLCIE
OrthoDBiEOG091G0OQD
PhylomeDBiQ8K3J1
TreeFamiTF105610

Family and domain databases

HAMAPiMF_01351 NDH1_NuoI, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR010226 NADH_quinone_OxRdtase_chainI
PfamiView protein in Pfam
PF12838 Fer4_7, 1 hit
TIGRFAMsiTIGR01971 NuoI, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3J1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM
60 70 80 90 100
KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AICPAQAITI EAEPRADGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE
210
IAANIQADYL YR
Length:212
Mass (Da):24,038
Last modified:October 1, 2002 - v1
Checksum:i1E06E024EA7829CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY096002 Genomic DNA Translation: AAM34451.1
CCDSiCCDS29402.1
PIRiPC7079
RefSeqiNP_001258372.1, NM_001271443.1
NP_001258373.1, NM_001271444.1
NP_659119.2, NM_144870.5
UniGeneiMm.44227

Genome annotation databases

EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734
GeneIDi225887
KEGGimmu:225887
UCSCiuc008fxn.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY096002 Genomic DNA Translation: AAM34451.1
CCDSiCCDS29402.1
PIRiPC7079
RefSeqiNP_001258372.1, NM_001271443.1
NP_001258373.1, NM_001271444.1
NP_659119.2, NM_144870.5
UniGeneiMm.44227

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6G2Jelectron microscopy3.30I1-212[»]
6G72electron microscopy3.90I1-212[»]
ProteinModelPortaliQ8K3J1
SMRiQ8K3J1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230440, 1 interactor
ComplexPortaliCPX-266 Mitochondrial respiratory chain complex I
CORUMiQ8K3J1
IntActiQ8K3J1, 4 interactors
MINTiQ8K3J1
STRINGi10090.ENSMUSP00000074600

PTM databases

iPTMnetiQ8K3J1
PhosphoSitePlusiQ8K3J1
SwissPalmiQ8K3J1

2D gel databases

REPRODUCTION-2DPAGEiQ8K3J1

Proteomic databases

EPDiQ8K3J1
MaxQBiQ8K3J1
PaxDbiQ8K3J1
PRIDEiQ8K3J1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734
GeneIDi225887
KEGGimmu:225887
UCSCiuc008fxn.2 mouse

Organism-specific databases

CTDi4728
MGIiMGI:2385079 Ndufs8

Phylogenomic databases

eggNOGiKOG3256 Eukaryota
COG1143 LUCA
GeneTreeiENSGT00390000003049
HOGENOMiHOG000228289
HOVERGENiHBG006547
InParanoidiQ8K3J1
KOiK03941
OMAiLCGLCIE
OrthoDBiEOG091G0OQD
PhylomeDBiQ8K3J1
TreeFamiTF105610

Enzyme and pathway databases

ReactomeiR-MMU-611105 Respiratory electron transport
R-MMU-6799198 Complex I biogenesis

Miscellaneous databases

PROiPR:Q8K3J1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059734 Expressed in 271 organ(s), highest expression level in brown adipose tissue
CleanExiMM_NDUFS8
ExpressionAtlasiQ8K3J1 baseline and differential
GenevisibleiQ8K3J1 MM

Family and domain databases

HAMAPiMF_01351 NDH1_NuoI, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR010226 NADH_quinone_OxRdtase_chainI
PfamiView protein in Pfam
PF12838 Fer4_7, 1 hit
TIGRFAMsiTIGR01971 NuoI, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS8_MOUSE
AccessioniPrimary (citable) accession number: Q8K3J1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2002
Last modified: September 12, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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