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UniProtKB - Q8K3F7 (TDH_MOUSE)
Protein
L-threonine 3-dehydrogenase, mitochondrial
Gene
Tdh
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the NAD+-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate, mediating L-threonine catabolism.
1 PublicationCatalytic activityi
- EC:1.1.1.1031 Publication
Kineticsi
- KM=72.4 mM for L-threonine1 Publication
- KM=1.67 mM for NAD+1 Publication
: L-threonine degradation via oxydo-reductase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycine from L-threonine.1 Publication This subpathway is part of the pathway L-threonine degradation via oxydo-reductase pathway, which is itself part of Amino-acid degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 195 | Proton donor/acceptor1 Publication | 1 | |
Binding sitei | 195 | NADCombined sources1 Publication | 1 | |
Binding sitei | 199 | NADCombined sources1 Publication | 1 | |
Binding sitei | 225 | NAD; via amide nitrogenCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 62 – 67 | NAD bindingCombined sources1 Publication | 6 | |
Nucleotide bindingi | 88 – 90 | NAD bindingCombined sources1 Publication | 3 | |
Nucleotide bindingi | 106 – 107 | NAD bindingCombined sources1 Publication | 2 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- L-threonine 3-dehydrogenase activity Source: UniProtKB
GO - Biological processi
- L-threonine catabolic process to glycine Source: UniProtKB-UniPathway
- threonine catabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.1.1.103, 3474 |
UniPathwayi | UPA00046;UER00505 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TdhImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926231, Tdh |
VEuPathDBi | HostDB:ENSMUSG00000021953 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: MGI
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 133 | S → A: Decreased L-threonine 3-dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 180 | R → K: Decreased L-threonine 3-dehydrogenase activity. No effect on protein NAD(+)-binding. No gross effect on protein folding. No effect on protein stability. 1 Publication | 1 | |
Mutagenesisi | 237 | T → A: Decreased L-threonine 3-dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 333 | M → A: Decreased L-threonine 3-dehydrogenase activity. 1 Publication | 1 | |
Mutagenesisi | 333 | M → E: Decreased L-threonine 3-dehydrogenase activity. Decreased affinity for L-threonine. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000298784 | ? – 373 | L-threonine 3-dehydrogenase, mitochondrial |
Proteomic databases
PaxDbi | Q8K3F7 |
PeptideAtlasi | Q8K3F7 |
PRIDEi | Q8K3F7 |
ProteomicsDBi | 262738 |
PTM databases
iPTMneti | Q8K3F7 |
PhosphoSitePlusi | Q8K3F7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021953, Expressed in yolk sac and 65 other tissues |
ExpressionAtlasi | Q8K3F7, baseline and differential |
Genevisiblei | Q8K3F7, MM |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 208451, 4 interactors |
STRINGi | 10090.ENSMUSP00000022522 |
Miscellaneous databases
RNActi | Q8K3F7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q8K3F7 |
SMRi | Q8K3F7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the NAD(P)-dependent epimerase/dehydratase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2774, Eukaryota |
GeneTreei | ENSGT00390000014037 |
HOGENOMi | CLU_007383_19_1_1 |
InParanoidi | Q8K3F7 |
OMAi | HWHASPR |
OrthoDBi | 1327602at2759 |
PhylomeDBi | Q8K3F7 |
TreeFami | TF314544 |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q8K3F7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLFLGMLKQV VNGTAQSKAS SCRKLVLPLK FLGTSQHRIP ADANFHSTSI
60 70 80 90 100
SEAEPPRVLI TGGLGQLGVG LANLLRKRFG KDNVILSDIR KPPAHVFHSG
110 120 130 140 150
PFVYANILDY KSLREIVVNH RISWLFHYSA LLSAVGEANV SLARDVNITG
160 170 180 190 200
LHNVLDVAAE YNVRLFVPST IGAFGPTSPR NPAPDLCIQR PRTIYGVSKV
210 220 230 240 250
HTELMGEYYY YRYGLDFRCL RYPGIISADS QPGGGTTDYA VQIFHAAAKN
260 270 280 290 300
GTFECNLEAG TRLPMMYISD CLRATLEVME APAERLSMRT YNISAMSFTP
310 320 330 340 350
EELAQALRKH APDFQITYCV DPLRQAIAES WPMILDDSNA RKDWGWKHDF
360 370
DLPELVATML NFHGVSTRVA QVN
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6X7T7 | F6X7T7_MOUSE | L-threonine 3-dehydrogenase, mitoch... | Tdh | 219 | Annotation score: | ||
F6Y7H0 | F6Y7H0_MOUSE | L-threonine 3-dehydrogenase, mitoch... | Tdh | 191 | Annotation score: | ||
F7AJL7 | F7AJL7_MOUSE | L-threonine 3-dehydrogenase, mitoch... | Tdh | 169 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 82 | D → N in AAH58860 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 214 | G → V in AAF61395 (PubMed:12097150).Curated | 1 | |
Sequence conflicti | 232 | P → R in AAF61395 (PubMed:12097150).Curated | 1 | |
Sequence conflicti | 318 | Y → H in AAH58860 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 361 | N → S in AAF61395 (PubMed:12097150).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF134346 mRNA Translation: AAF61395.2 AY116662 mRNA Translation: AAM51557.1 AK077571 mRNA Translation: BAC36870.1 BC058860 mRNA Translation: AAH58860.1 |
CCDSi | CCDS27202.1 |
RefSeqi | NP_067455.5, NM_021480.5 |
Genome annotation databases
Ensembli | ENSMUST00000022522; ENSMUSP00000022522; ENSMUSG00000021953 |
GeneIDi | 58865 |
KEGGi | mmu:58865 |
UCSCi | uc007uhs.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF134346 mRNA Translation: AAF61395.2 AY116662 mRNA Translation: AAM51557.1 AK077571 mRNA Translation: BAC36870.1 BC058860 mRNA Translation: AAH58860.1 |
CCDSi | CCDS27202.1 |
RefSeqi | NP_067455.5, NM_021480.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4YR9 | X-ray | 2.80 | A/B/C/D/E/F | 47-373 | [»] | |
4YRA | X-ray | 2.65 | A/B/C/D/E/F/G/H/I/J/K/L | 47-373 | [»] | |
4YRB | X-ray | 3.25 | A/B/C/D/E/F | 47-373 | [»] | |
AlphaFoldDBi | Q8K3F7 | |||||
SMRi | Q8K3F7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 208451, 4 interactors |
STRINGi | 10090.ENSMUSP00000022522 |
PTM databases
iPTMneti | Q8K3F7 |
PhosphoSitePlusi | Q8K3F7 |
Proteomic databases
PaxDbi | Q8K3F7 |
PeptideAtlasi | Q8K3F7 |
PRIDEi | Q8K3F7 |
ProteomicsDBi | 262738 |
Protocols and materials databases
DNASUi | 58865 |
Genome annotation databases
Ensembli | ENSMUST00000022522; ENSMUSP00000022522; ENSMUSG00000021953 |
GeneIDi | 58865 |
KEGGi | mmu:58865 |
UCSCi | uc007uhs.1, mouse |
Organism-specific databases
CTDi | 157739 |
MGIi | MGI:1926231, Tdh |
VEuPathDBi | HostDB:ENSMUSG00000021953 |
Phylogenomic databases
eggNOGi | KOG2774, Eukaryota |
GeneTreei | ENSGT00390000014037 |
HOGENOMi | CLU_007383_19_1_1 |
InParanoidi | Q8K3F7 |
OMAi | HWHASPR |
OrthoDBi | 1327602at2759 |
PhylomeDBi | Q8K3F7 |
TreeFami | TF314544 |
Enzyme and pathway databases
UniPathwayi | UPA00046;UER00505 |
BRENDAi | 1.1.1.103, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 58865, 2 hits in 70 CRISPR screens |
ChiTaRSi | Tdh, mouse |
PROi | PR:Q8K3F7 |
RNActi | Q8K3F7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021953, Expressed in yolk sac and 65 other tissues |
ExpressionAtlasi | Q8K3F7, baseline and differential |
Genevisiblei | Q8K3F7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TDH_MOUSE | |
Accessioni | Q8K3F7Primary (citable) accession number: Q8K3F7 Secondary accession number(s): Q6PD91, Q9JLU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 21, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families