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Protein

F-box/SPRY domain-containing protein 1

Gene

Fbxo45

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration. Plays a role in the regulation of neurotransmission at mature neurons (By similarity). May control synaptic activity by controlling UNC13A via ubiquitin dependent pathway. Specifically recognizes TP73, promoting its ubiquitination and degradation (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • anterior commissure morphogenesis Source: MGI
  • cellular response to DNA damage stimulus Source: MGI
  • cerebral cortex radially oriented cell migration Source: MGI
  • cerebral cortex tangential migration Source: MGI
  • corticospinal tract morphogenesis Source: MGI
  • innervation Source: MGI
  • neuron migration Source: MGI
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • synapse assembly involved in innervation Source: MGI
  • ubiquitin-dependent protein catabolic process Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processNeurogenesis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/SPRY domain-containing protein 1
Alternative name(s):
F-box only protein 45
Short name:
mFbxo45
Gene namesi
Name:Fbxo45Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2447775 Fbxo45

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Homozygous animals die soon after birth due to respiratory distress. Embryos show abnormal innervation of the diaphragm, impaired synapse formation at neuromuscular junctions, and aberrant development of axon fiber tracts in the brain.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42R → E: Allows binding to CUL1 and RBX1 and formation of an authentic SCF (SKP1-CUL1-F-box protein) complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001199472 – 286F-box/SPRY domain-containing protein 1Add BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8K3B1
MaxQBiQ8K3B1
PaxDbiQ8K3B1
PeptideAtlasiQ8K3B1
PRIDEiQ8K3B1

PTM databases

PhosphoSitePlusiQ8K3B1

Expressioni

Tissue specificityi

Expressed speciffically in the central nervous system, including cerebellum, medulla oblongata, olfactory bulb, hippocampus, cortex and brain stem.2 Publications

Gene expression databases

BgeeiENSMUSG00000035764
CleanExiMM_FBXO45
GenevisibleiQ8K3B1 MM

Interactioni

Subunit structurei

Forms a complex with MYCBP2 and SKP1 but does not appear to form an authentic SCF (SKP1-CUL1-F-box protein) complex. Interacts (via B30.2/SPRY domain) with TP73 (via SAM domain); leading to its ubiquitination and degradation (By similarity). Interacts (via SRY domain) with UNC13A; leading to its degradation by the proteasome.By similarity2 Publications

Protein-protein interaction databases

BioGridi234572, 4 interactors
IntActiQ8K3B1, 2 interactors
STRINGi10090.ENSMUSP00000040168

Structurei

3D structure databases

ProteinModelPortaliQ8K3B1
SMRiQ8K3B1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 82F-boxPROSITE-ProRule annotationAdd BLAST50
Domaini92 – 284B30.2/SPRYPROSITE-ProRule annotationAdd BLAST193

Sequence similaritiesi

Belongs to the FBXO45/Fsn family.Curated

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG056500
InParanoidiQ8K3B1
KOiK10319
OMAiEIWWEGP
OrthoDBiEOG091G0DF0
PhylomeDBiQ8K3B1
TreeFamiTF312822

Family and domain databases

CDDicd12907 SPRY_Fbox, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR003877 SPRY_dom
IPR035784 SPRY_FBXO45
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50181 FBOX, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGPGAGA ASGGASGGGA GAGGGASAGS GSSGVGGRLP SRVLELVFSY
60 70 80 90 100
LELSELRSCA LVCKHWYRCL HGDENSEVWR SLCARSLAEE ALRTDILCNL
110 120 130 140 150
PSYKAKVRAF QHAFSTNDCS RNVYIKKNGF TLHRNPIAQS TDGARTKIGF
160 170 180 190 200
SEGRHAWEVW WEGPLGTVAV IGIATKRAPM QCQGYVALLG SDDQSWGWNL
210 220 230 240 250
VDNNLLHNGE VNGSFPQCNN APKYQIGERI RVILDMEDKT LAFERGYEFL
260 270 280
GVAFRGLPKA CLYPAVSAVY GNTEVTLVYL GKPLDG
Length:286
Mass (Da):30,605
Last modified:October 25, 2005 - v2
Checksum:i78589911B5DA31B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27S → C in AAH26799 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026799 mRNA Translation: AAH26799.1
BC088738 mRNA Translation: AAH88738.1
BC098203 mRNA Translation: AAH98203.1
CCDSiCCDS28117.1
RefSeqiNP_775615.2, NM_173439.2
UniGeneiMm.256137

Genome annotation databases

EnsembliENSMUST00000042732; ENSMUSP00000040168; ENSMUSG00000035764
GeneIDi268882
KEGGimmu:268882
UCSCiuc007yyl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiFBSP1_MOUSE
AccessioniPrimary (citable) accession number: Q8K3B1
Secondary accession number(s): Q5M7B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: May 23, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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