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Protein

CDK5 regulatory subunit-associated protein 2

Gene

Cdk5rap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion (PubMed:20627074). Involved in regulation of mitotic spindle orientation (PubMed:20460369). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Required for the recruitment of AKAP9 to centrosomes (By similarity). Plays a role in neurogenesis (PubMed:20471352). Contrary to higher mammalian orthologs, including human, chimpanzee, bovine and dog, does not interact with EB1/MAPRE1, therefore its function in the regulation of microtubule dynamics is unclear (PubMed:19553473).By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
CDK5 regulatory subunit-associated protein 2
Alternative name(s):
CDK5 activator-binding protein C48
Gene namesi
Name:Cdk5rap2
Synonyms:Kiaa1633
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2384875 Cdk5rap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000898371 – 1822CDK5 regulatory subunit-associated protein 2Add BLAST1822

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485PhosphoserineCombined sources1
Modified residuei544PhosphoserineBy similarity1
Modified residuei1195PhosphothreonineCombined sources1
Modified residuei1243PhosphoserineBy similarity1
Modified residuei1245PhosphoserineCombined sources1
Modified residuei1497PhosphoserineCombined sources1
Modified residuei1592PhosphoserineBy similarity1
Modified residuei1595PhosphoserineBy similarity1
Modified residuei1822PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in vitro by CDK5.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K389
MaxQBiQ8K389
PaxDbiQ8K389
PRIDEiQ8K389

PTM databases

iPTMnetiQ8K389
PhosphoSitePlusiQ8K389

Expressioni

Tissue specificityi

Expressed in testis, thymus, heart and brain.1 Publication

Developmental stagei

Detected in the developing cortex at 9.5 dpc. Expression peaks between 10.5 and 13.5 dpc, remains robust at 15.5 dpc and declines thereafter. This peak corresponds to periods of active neurogenesis.2 Publications

Gene expression databases

BgeeiENSMUSG00000039298 Expressed in 201 organ(s), highest expression level in testis
CleanExiMM_CDK5RAP2
ExpressionAtlasiQ8K389 baseline and differential
GenevisibleiQ8K389 MM

Interactioni

Subunit structurei

Interacts with CDK5R1 (p35 form) (By similarity). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. May form a complex with CDK5R1 and CDK5 (By similarity). Interacts (via C-terminus) with PCNT; the interaction is leading to centrosomal localization of CDK5RAP2 and PCNT. Interacts with AKAP9; the interaction is leading to Golgi localization of CDK5RAP2 and AKAP9 (By similarity). Interacts with TUBG1; the interaction is leading to the centrosomal localization of CDK5RAP2 and TUBG1 (By similarity). Interacts with TUBGCP3 (By similarity). Interacts with CALM1 (By similarity). Interacts with CDC20 (By similarity). Interacts with CEP68; degradation of CEP68 in early mitosis leads to removal of CDK5RAP2 from the centrosome which promotes centriole disengagement and subsequent centriole separation (By similarity). Interacts with NCKAP5L (By similarity). Interacts with LGALS3BP; this interaction may connect the pericentrosomal complex to the gamma-tubulin ring complex (gamma-TuRC) to promote microtubule assembly and acetylation (By similarity). Contrary to human, chimpanzee, bovine and dog orthologous proteins, does not interact with EB1/MAPRE1, possibly due to a divergence at the level of the critical residue 940, which is a proline in MAPRE1-binding orthologs and a leucine in mouse and rat (PubMed:19553473).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229527, 20 interactors
IntActiQ8K389, 20 interactors
STRINGi10090.ENSMUSP00000119891

Structurei

3D structure databases

SMRiQ8K389
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 195Interaction with NCKAP5LBy similarityAdd BLAST139
Regioni1201 – 1822Interaction with PCNT and AKAP91 PublicationAdd BLAST622
Regioni1655 – 1822Required for centrosomal attachment, Golgi targeting and CALM1 interactionBy similarityAdd BLAST168
Regioni1655 – 1697Interaction with CDK5R1By similarityAdd BLAST43
Regioni1688 – 1822Interaction with PCNT1 PublicationAdd BLAST135
Regioni1790 – 1799Required for centrosomal attachment, Golgi localization and CALM1 interactionBy similarity10

Phylogenomic databases

eggNOGiENOG410J2NR Eukaryota
ENOG411281S LUCA
GeneTreeiENSGT00530000063845
HOVERGENiHBG050976
InParanoidiQ8K389
KOiK16542
OMAiSEICPPD
OrthoDBiEOG091G008W
PhylomeDBiQ8K389
TreeFamiTF329233

Family and domain databases

InterProiView protein in InterPro
IPR012943 Cnn_1N
PfamiView protein in Pfam
PF07989 Cnn_1N, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q8K389-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSGMEEEGA LPGTLSGCSG LHPVLPSDLD VISDTSGLGN GVLPSMSEEK
60 70 80 90 100
VSPTRARNMK DFENQITELK KENFNLKLRI YFLEERIQQE FAGPTEHIYK
110 120 130 140 150
TNIELKVEVE SLKRELQEKD QLLVKASKAV ESLAERGGSE VQRVKEDARK
160 170 180 190 200
KVQQVEDLLT KRIHLLEEDV KAAQAELEKA FAGTETEKAL RLSLESKLSA
210 220 230 240 250
MKKMQEGDLE MTLALEEKDR LIEELKLSLK SKEALIQCLK EEKSQMASPD
260 270 280 290 300
ENVSSGELRG LSATLREEKE RDAEEWQKER NHFEERIQAL QEDLREKERE
310 320 330 340 350
IATEKKNNLK SYKAIQGLTM ALKSKEREVE ELDSKIKEVT TDSTKGREDP
360 370 380 390 400
LKTQIPRFQL REGSEDCEAA LVEKEALLAK LHSENVTKST ENHRLLRNVK
410 420 430 440 450
KVTQELNDLK KEKLRLEQAL EEAHQEGNRG ARTIHDLRNE VEKLRKEVSE
460 470 480 490 500
REKAVEKHYK SLPGESKTKF HTQEQVVRSL TGSGSQEDLL LQKSNEKDLE
510 520 530 540 550
AIQQNCYLMT AEELKFGSDG LITEKCSQQS PDSKLIFSKE KQQSEYEGLT
560 570 580 590 600
GDLKAEQNIY AHLAKTQDTD SVSNLQAELK EVLALRKQLE QDVLAYRNLQ
610 620 630 640 650
KALQEQLSEI RSREEEPFSF YSDQTSYLSI CLEEHNQFQL EHFSQEELKK
660 670 680 690 700
KVSDLIQLVK DLHTDNQHLK KTIFDLSSVG FQGSDRLELT KQEELVASKE
710 720 730 740 750
DEDTLKFEAD VETPFQSDQH LEQSREIMED YAEGGCKSGY GRHMDSNILG
760 770 780 790 800
HDGAQTPGAS EEHTLEDELL GLLATLFSKK ATPLLESRPD LLKALGALLL
810 820 830 840 850
ERICLAEQGR PGDHLDSKTE KALQQVAVQL RDELGHSFPA NSFSKSYNEV
860 870 880 890 900
KSMWGNWLVK TGDEDTVELK SVSVQTMAIE DTPHGFKPQS KRDAWAEKQE
910 920 930 940 950
EAIFSTELES EALGEMPGQQ ATHLSFPSAI NPDAEKTGLL IQLKTPELLE
960 970 980 990 1000
NLYNLPASPE VVVAQLQGQV LELQRELKEF KTRNKQLHEK LILAEAMMEG
1010 1020 1030 1040 1050
LPVPNSALVN VPAAQAVVRT AFQDNPGEQE GPETTQSAGR DKDMDSDQYT
1060 1070 1080 1090 1100
SFEIDSEICP PDDLALLPAC KENLEDLLGP SSIATYLDSK SQLSVKVSVN
1110 1120 1130 1140 1150
GTDQSENINI PDDTEDLKQK IHDLQTELEG YRNIIVQLLK HSQCSEAIIT
1160 1170 1180 1190 1200
VLCGTEGAQD GLNKPKGHID EEEMTFSSLH QVRYVKHMKI LRPLTPEMID
1210 1220 1230 1240 1250
GKMLESLKQQ LVDQEQELQK EQDLNLELFG EIHDLQNKFQ DLSPSRYDSL
1260 1270 1280 1290 1300
VQSQARELSL QRQQIKDSHG ICVIYRQHMS TMIKAFEELL QASDVDSCVA
1310 1320 1330 1340 1350
EGFREQLTQC AGLLEQLERL FLHGKSARVE PHPQNELLKG LRTVEGNLPY
1360 1370 1380 1390 1400
HHLLPESPEP SASHALSDDE MSEKSFLSRD PKPDSDTEKY PAMASHFPQD
1410 1420 1430 1440 1450
LLMEHIQEIR TLRKHLEESI KTNEKLRKQL ERQGSETDQG SRNVSACGLA
1460 1470 1480 1490 1500
LHSSLTSEIH FLRKQNEALS MMLEKGSKDK QKESEKLRES LARKAESLEQ
1510 1520 1530 1540 1550
LQLEYTSVRE ENERLQRDII EKERHNQELT EEVCSSRQEL SRVQEEAKSR
1560 1570 1580 1590 1600
QQLLSQKDKL LQSLQMELKV YEKLAEEHPR LQQDGSKCPE ASDNSFDLFE
1610 1620 1630 1640 1650
STQAMAPKSA SETPLLSGTD VDSLSCDSTS SATSPTSMPC LVAGHHMWAS
1660 1670 1680 1690 1700
KSGHHMLGLI EDYDALYKQI SWGQTLLAKM DVQTQEALSP TSHKLGPKGS
1710 1720 1730 1740 1750
SSVPLSKFLS SMNTAKLVLE KASRLLKLFW RVSVPTNGQC SLHCEQIGEM
1760 1770 1780 1790 1800
KAENTKLHKK LFEQEKKLQN TAKLLQQSKH QEKVIFDQLV ITHQVLRKAR
1810 1820
GNLELRPGAT RPGASSPSRP GS
Length:1,822
Mass (Da):205,945
Last modified:December 20, 2005 - v3
Checksum:iDDD6CD7B8CFC8DA9
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SP75Q5SP75_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap2
390Annotation score:
F6R2K5F6R2K5_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap2
92Annotation score:
H7BX43H7BX43_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap2
76Annotation score:

Sequence cautioni

The sequence BAC98221 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129411 mRNA Translation: BAC98221.1 Different initiation.
AL929409, AL845502 Genomic DNA Translation: CAM23429.1
BC059253 mRNA Translation: AAH59253.1
CCDSiCCDS38779.1
RefSeqiNP_666102.2, NM_145990.4
UniGeneiMm.370777
Mm.379344

Genome annotation databases

EnsembliENSMUST00000144099; ENSMUSP00000119891; ENSMUSG00000039298
GeneIDi214444
KEGGimmu:214444
UCSCiuc008thy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129411 mRNA Translation: BAC98221.1 Different initiation.
AL929409, AL845502 Genomic DNA Translation: CAM23429.1
BC059253 mRNA Translation: AAH59253.1
CCDSiCCDS38779.1
RefSeqiNP_666102.2, NM_145990.4
UniGeneiMm.370777
Mm.379344

3D structure databases

SMRiQ8K389
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229527, 20 interactors
IntActiQ8K389, 20 interactors
STRINGi10090.ENSMUSP00000119891

PTM databases

iPTMnetiQ8K389
PhosphoSitePlusiQ8K389

Proteomic databases

EPDiQ8K389
MaxQBiQ8K389
PaxDbiQ8K389
PRIDEiQ8K389

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000144099; ENSMUSP00000119891; ENSMUSG00000039298
GeneIDi214444
KEGGimmu:214444
UCSCiuc008thy.1 mouse

Organism-specific databases

CTDi55755
MGIiMGI:2384875 Cdk5rap2
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410J2NR Eukaryota
ENOG411281S LUCA
GeneTreeiENSGT00530000063845
HOVERGENiHBG050976
InParanoidiQ8K389
KOiK16542
OMAiSEICPPD
OrthoDBiEOG091G008W
PhylomeDBiQ8K389
TreeFamiTF329233

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

ChiTaRSiCdk5rap2 mouse
PROiPR:Q8K389
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039298 Expressed in 201 organ(s), highest expression level in testis
CleanExiMM_CDK5RAP2
ExpressionAtlasiQ8K389 baseline and differential
GenevisibleiQ8K389 MM

Family and domain databases

InterProiView protein in InterPro
IPR012943 Cnn_1N
PfamiView protein in Pfam
PF07989 Cnn_1N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCK5P2_MOUSE
AccessioniPrimary (citable) accession number: Q8K389
Secondary accession number(s): A2AVA1, Q6PCN1, Q6ZPL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 20, 2005
Last modified: September 12, 2018
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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