Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DENN domain-containing protein 1A

Gene

Dennd1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes (PubMed:17182770, PubMed:20159556). Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns3P (PubMed:20159556).2 Publications

Activity regulationi

The guanine nucleotide exchange factor (GEF) activity is autoinhibited. Autoinhibition may be the result of intramolecular interaction between the DENN domain and the C-terminus, which is disrupted upon phosphorylation. Activation is regulated by Akt activation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
Biological processProtein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 1A
Alternative name(s):
Connecdenn 11 Publication
Short name:
Connecdenn1 Publication
Gene namesi
Name:Dennd1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2442794 Dennd1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002426811 – 1016DENN domain-containing protein 1AAdd BLAST1016

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei473PhosphoserineCombined sources1
Modified residuei519PhosphothreonineBy similarity1
Modified residuei520PhosphoserineCombined sources1
Modified residuei522PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1
Modified residuei536PhosphoserineBy similarity1
Modified residuei538PhosphoserineCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei592PhosphoserineBy similarity1
Modified residuei750PhosphoserineBy similarity1
Modified residuei760Omega-N-methylarginineCombined sources1

Post-translational modificationi

Phosphorylated on serine and/or threonine in an Akt-dependent manner. Phosphorylation probably regulates the guanine nucleotide exchange factor (GEF) activity, possibly by disrupting an intramolecular interaction between the DENN domain and the C-terminus of the protein, thereby relieving the autoinhibition.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ8K382
MaxQBiQ8K382
PaxDbiQ8K382
PRIDEiQ8K382

PTM databases

iPTMnetiQ8K382
PhosphoSitePlusiQ8K382

Expressioni

Gene expression databases

BgeeiENSMUSG00000035392 Expressed in 248 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_DENND1A
ExpressionAtlasiQ8K382 baseline and differential
GenevisibleiQ8K382 MM

Interactioni

Subunit structurei

Interacts with RAB35 (PubMed:20159556). Interacts with clathrin and with the adapter protein complex 2, AP-2 (By similarity). Interacts with ITSN1 AND SH3GL2 (PubMed:17182770). Interacts (when phosphorylated) with YWHAE (By similarity).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8K382, 2 interactors
MINTiQ8K382
STRINGi10090.ENSMUSP00000099848

Structurei

3D structure databases

ProteinModelPortaliQ8K382
SMRiQ8K382
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 145uDENNPROSITE-ProRule annotationAdd BLAST133
Domaini162 – 298cDENNPROSITE-ProRule annotationAdd BLAST137
Domaini300 – 378dDENNPROSITE-ProRule annotationAdd BLAST79

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi381 – 385FXDXF motifBy similarity5
Motifi569 – 578Clathrin boxBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi649 – 1000Pro-richAdd BLAST352

Phylogenomic databases

eggNOGiKOG3569 Eukaryota
ENOG410XT3N LUCA
GeneTreeiENSGT00760000118819
HOGENOMiHOG000060237
HOVERGENiHBG059210
InParanoidiQ8K382
KOiK20160
OMAiDDECQRE
OrthoDBiEOG091G083L
PhylomeDBiQ8K382
TreeFamiTF343037

Family and domain databases

InterProiView protein in InterPro
IPR001194 cDENN_dom
IPR005112 dDENN_dom
IPR040032 DENND1A/B/C
IPR037516 Tripartite_DENN
IPR005113 uDENN_dom
PANTHERiPTHR13196 PTHR13196, 2 hits
PfamiView protein in Pfam
PF03455 dDENN, 1 hit
PF02141 DENN, 1 hit
PF03456 uDENN, 1 hit
SMARTiView protein in SMART
SM00801 dDENN, 1 hit
SM00799 DENN, 1 hit
SM00800 uDENN, 1 hit
PROSITEiView protein in PROSITE
PS50211 DENN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q8K382-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSRIKQNPE TTFEVYVEVA YPRTGGTLSD PEVQRQFPED YSDQEVLQTL
60 70 80 90 100
TKFCFPFYVD SLTVSQVGQN FTFVLTDIDS KQRFGFCRLS SGAKSCFCIL
110 120 130 140 150
SYLPWFEVFY KLLNILADYT TKRQESQWNE LLETLHRLPI PDPGVSVHLS
160 170 180 190 200
VHSYFTVPDS RELPSIPENR NLTEYFVAVD VNNMLHLYAS MLYERRILII
210 220 230 240 250
CSKLSTLTAC IHGSAAMLYP MYWQHVYIPV LPPHLLDYCC APMPYLIGIH
260 270 280 290 300
LSLMEKVRNM ALDDVVILNV DTNTLETPFD DLQSLPNDVI SSLKNRLKKV
310 320 330 340 350
STTTGDGVAR AFLKAQAAFF GSYRNALKIE PEEPITFSEE AFVSHYRSGA
360 370 380 390 400
MKQFLQNATQ LQLFKQFIDG RLDLLNSGEG FSDVFEEEIN MGEYAGSDKL
410 420 430 440 450
YHQWLSTVRK GSGAILNTVK TKANPAMKTV YKFAKDHAKM GIKEVKNRLK
460 470 480 490 500
QKDITENGCV SSAEDPLPKT MPSPQAETQD PRLREDRRPI TVHFGQVRPP
510 520 530 540 550
RPHVVRRPKS NITVEGRRTS VSSPEQPQPY RTLKESDSAE GDETESPEQL
560 570 580 590 600
VREPWGPTPA PPDRAASIDL LEDVFSSLDV EAPLQPLGQA KSLEDLRAPK
610 620 630 640 650
DLREQPGSFD YQRLDLCRSE RGLSMAAALK LAHPYTKLWS LGQDDMAIPS
660 670 680 690 700
KPSITSPEKP SALLGTSPAL PLRPQNQEGI LSPSIKEETP IPTPGSITIP
710 720 730 740 750
RPQGRKTPEL GIVPPPPTAR PAKLQAAGGP LGDFSSEPLQ MDRERQAALS
760 770 780 790 800
PALLSGLLPR AVPQGPTELL QPPSPAPGAA GTGSDALLAL LDPLNTAWSG
810 820 830 840 850
STIPSHPATP SAATPFIPQL SFPPTVTPTP FVQTPLNPFV PSVPVVPPSM
860 870 880 890 900
PLSSTPARPF GTPPASLGPA YAPSILLSSS GFYAPHRSQP NLSALSMPNL
910 920 930 940 950
FGQIPMGAHT SPLQPLGPPA VAPSRIRTLP LARSSARAAE AKQGLALRPG
960 970 980 990 1000
ESPLLPPRPP QSLQPTPQPS VPTQARDPFE DLLRKTKQDV SPSPAPALAP
1010
ASTSVEQLRR QWETFE
Length:1,016
Mass (Da):111,538
Last modified:July 1, 2008 - v2
Checksum:i352A1A6F940D7382
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RH88D6RH88_MOUSE
DENN domain-containing protein 1A
Dennd1a
103Annotation score:
A2AUP6A2AUP6_MOUSE
DENN domain-containing protein 1A
Dennd1a
105Annotation score:
D6RI65D6RI65_MOUSE
DENN domain-containing protein 1A
Dennd1a
47Annotation score:
A2ALU3A2ALU3_MOUSE
DENN domain-containing protein 1A
Dennd1a
277Annotation score:
D6RHP6D6RHP6_MOUSE
DENN domain-containing protein 1A
Dennd1a
80Annotation score:
D6RHL0D6RHL0_MOUSE
DENN domain-containing protein 1A
Dennd1a
49Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152H → Y in ABD93329 (PubMed:17182770).Curated1
Sequence conflicti152H → Y in AAH27786 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ448594 mRNA Translation: ABD93329.1
AL805959
, AL928810, AL929186, AL929238 Genomic DNA Translation: CAM22966.1
AL929186
, AL805959, AL928810, AL929238 Genomic DNA Translation: CAM23322.1
AL929238
, AL805959, AL928810, AL929186 Genomic DNA Translation: CAM25676.1
AL928810
, AL805959, AL929186, AL929238 Genomic DNA Translation: CAM26757.1
BC027786 mRNA Translation: AAH27786.1
CCDSiCCDS16007.1
RefSeqiNP_666234.3, NM_146122.3
UniGeneiMm.314245
Mm.461078

Genome annotation databases

EnsembliENSMUST00000102787; ENSMUSP00000099848; ENSMUSG00000035392
GeneIDi227801
KEGGimmu:227801
UCSCiuc008jng.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ448594 mRNA Translation: ABD93329.1
AL805959
, AL928810, AL929186, AL929238 Genomic DNA Translation: CAM22966.1
AL929186
, AL805959, AL928810, AL929238 Genomic DNA Translation: CAM23322.1
AL929238
, AL805959, AL928810, AL929186 Genomic DNA Translation: CAM25676.1
AL928810
, AL805959, AL929186, AL929238 Genomic DNA Translation: CAM26757.1
BC027786 mRNA Translation: AAH27786.1
CCDSiCCDS16007.1
RefSeqiNP_666234.3, NM_146122.3
UniGeneiMm.314245
Mm.461078

3D structure databases

ProteinModelPortaliQ8K382
SMRiQ8K382
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K382, 2 interactors
MINTiQ8K382
STRINGi10090.ENSMUSP00000099848

PTM databases

iPTMnetiQ8K382
PhosphoSitePlusiQ8K382

Proteomic databases

EPDiQ8K382
MaxQBiQ8K382
PaxDbiQ8K382
PRIDEiQ8K382

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102787; ENSMUSP00000099848; ENSMUSG00000035392
GeneIDi227801
KEGGimmu:227801
UCSCiuc008jng.2 mouse

Organism-specific databases

CTDi57706
MGIiMGI:2442794 Dennd1a

Phylogenomic databases

eggNOGiKOG3569 Eukaryota
ENOG410XT3N LUCA
GeneTreeiENSGT00760000118819
HOGENOMiHOG000060237
HOVERGENiHBG059210
InParanoidiQ8K382
KOiK20160
OMAiDDECQRE
OrthoDBiEOG091G083L
PhylomeDBiQ8K382
TreeFamiTF343037

Enzyme and pathway databases

ReactomeiR-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiDennd1a mouse
PROiPR:Q8K382
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035392 Expressed in 248 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_DENND1A
ExpressionAtlasiQ8K382 baseline and differential
GenevisibleiQ8K382 MM

Family and domain databases

InterProiView protein in InterPro
IPR001194 cDENN_dom
IPR005112 dDENN_dom
IPR040032 DENND1A/B/C
IPR037516 Tripartite_DENN
IPR005113 uDENN_dom
PANTHERiPTHR13196 PTHR13196, 2 hits
PfamiView protein in Pfam
PF03455 dDENN, 1 hit
PF02141 DENN, 1 hit
PF03456 uDENN, 1 hit
SMARTiView protein in SMART
SM00801 dDENN, 1 hit
SM00799 DENN, 1 hit
SM00800 uDENN, 1 hit
PROSITEiView protein in PROSITE
PS50211 DENN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDEN1A_MOUSE
AccessioniPrimary (citable) accession number: Q8K382
Secondary accession number(s): A0MCI0, A2ALU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 1, 2008
Last modified: November 7, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again