Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chromosome alignment-maintaining phosphoprotein 1

Gene

Champ1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri728 – 750C2H2-typePROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome alignment-maintaining phosphoprotein 1
Alternative name(s):
Zinc finger protein 828
Gene namesi
Name:Champ1
Synonyms:D8Ertd457e, Kiaa1802, Zfp828, Znf828
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1196398 Champ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483201 – 802Chromosome alignment-maintaining phosphoprotein 1Add BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei108PhosphoserineBy similarity1
Modified residuei204PhosphoserineBy similarity1
Modified residuei207PhosphoserineBy similarity1
Modified residuei234PhosphoserineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei243PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1
Modified residuei254PhosphoserineCombined sources1
Modified residuei265PhosphoserineBy similarity1
Modified residuei272PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei309PhosphoserineCombined sources1
Modified residuei334PhosphoserineBy similarity1
Modified residuei345PhosphoserineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei371PhosphothreonineCombined sources1
Modified residuei375PhosphoserineBy similarity1
Modified residuei394PhosphoserineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei421PhosphoserineBy similarity1
Modified residuei425PhosphoserineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei434PhosphoserineCombined sources1
Modified residuei441PhosphoserineCombined sources1
Modified residuei447PhosphothreonineBy similarity1
Modified residuei448PhosphoserineBy similarity1
Modified residuei451PhosphoserineBy similarity1
Modified residuei461PhosphoserineBy similarity1
Modified residuei479N6-acetyllysine; alternateBy similarity1
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei497PhosphoserineBy similarity1
Modified residuei502PhosphoserineBy similarity1
Modified residuei532PhosphoserineBy similarity1
Cross-linki555Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei593PhosphothreonineCombined sources1
Cross-linki596Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei603PhosphoserineCombined sources1
Modified residuei605PhosphoserineBy similarity1
Modified residuei617PhosphoserineCombined sources1
Modified residuei622PhosphoserineCombined sources1
Modified residuei641PhosphoserineCombined sources1
Modified residuei642PhosphoserineCombined sources1
Modified residuei643PhosphoserineCombined sources1
Cross-linki660Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei665PhosphoserineBy similarity1
Cross-linki679Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei726PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8K327
MaxQBiQ8K327
PaxDbiQ8K327
PeptideAtlasiQ8K327
PRIDEiQ8K327

PTM databases

iPTMnetiQ8K327
PhosphoSitePlusiQ8K327

Expressioni

Gene expression databases

BgeeiENSMUSG00000047710 Expressed in 251 organ(s), highest expression level in embryo
CleanExiMM_ZFP828
ExpressionAtlasiQ8K327 baseline and differential
GenevisibleiQ8K327 MM

Interactioni

Subunit structurei

Interacts with MAD2L2. Interacts with POGZ, CBX1, CBX3 and CBX5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi221774, 2 interactors
IntActiQ8K327, 2 interactors
MINTiQ8K327
STRINGi10090.ENSMUSP00000057270

Structurei

3D structure databases

ProteinModelPortaliQ8K327
SMRiQ8K327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni261 – 479Mediates interaction with MAD2L2By similarityAdd BLAST219
Regioni440 – 580Mediates localization to the spindle and the kinetochore and is required for the attachment of spindle microtubules to the kinetochoreBy similarityAdd BLAST141
Regioni581 – 802Mediates localization to the chromosome and the spindle and negatively regulates chromosome alignmentBy similarityAdd BLAST222

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi109 – 466Pro-richAdd BLAST358

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri728 – 750C2H2-typePROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IFC8 Eukaryota
ENOG410XXII LUCA
GeneTreeiENSGT00730000111351
HOGENOMiHOG000065731
HOVERGENiHBG062352
InParanoidiQ8K327
KOiK22593
OMAiTSKMYSN
OrthoDBiEOG091G02UO
PhylomeDBiQ8K327
TreeFamiTF350859

Family and domain databases

InterProiView protein in InterPro
IPR039330 CAMP
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR37354 PTHR37354, 1 hit
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8K327-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEVCQELRKP ALSLECGHCS FRGTDYENVQ LHMGSIHPEF CDDMDAGGLG
60 70 80 90 100
KLIFYQKSAK LFHCHKCFFT SKLYANVYYH ITARHAASDK WSEQPKEQPS
110 120 130 140 150
KDTESGKSPS PPERQNPAFD PAEARPTPAL PMEAQKTSPS LCPESQASGP
160 170 180 190 200
PVLEPQGAGP LISPEPQAPC LPAEASKAAP VPCPERVDPP CELPELEKPE
210 220 230 240 250
RGPSPESVKS ALVSSKPPKH SSFADTGAAP SALSPESPVL ATSPEPWGPS
260 270 280 290 300
LSASPESRKP ARTASPEPRK PSPAESPELW KPFPAIASEP RRPTPAVSPG
310 320 330 340 350
SWKPGPPGSP RPWKSSPSAT SGPWKSSKPV QPMSPGPWKP IPSVSPGPWK
360 370 380 390 400
PAPSMSTASW KSSVSSGSWK TPPTSPESWK SGPPELRKTA LPLSPEHWKA
410 420 430 440 450
VPPVSPELRR PGPPLSPEIR SPAGSPELKK PSSSPDLWKV SPDQRKTSPA
460 470 480 490 500
SLDFPEPQKS SCGSPPDLWK SSFIMESQKP NVFSETRKHT ASGSSESPKV
510 520 530 540 550
ASDIWKPVLS IDAEPRKSTL FPEPTKAVLP ASPEPRKRAL FPESRKHVFL
560 570 580 590 600
PELPKSAVFS DAQKAPELSE EIQLEAVDNA KCDSLAQEGL LATPKKLLDE
610 620 630 640 650
ALSPSSKKLK KDSQENSDAE LSSSEYIRAD LDTLDTKGQE SSSDQEQVDV
660 670 680 690 700
ESIDFSKENK MEMGSTEQAK NVLQFTEEKE AFISEEEIAK YMKRGKGKYY
710 720 730 740 750
CKICCCRAMK KGAVLHHLVN KHNVHSPYKC TICGKAFLLE SLLKNHVAAH
760 770 780 790 800
GQSLLKCPRC NFESNFPRGF KKHLTHCQSR HNEEVNKKLM EALESPLEEQ

QI
Length:802
Mass (Da):87,561
Last modified:October 1, 2002 - v1
Checksum:iD163571FE4492EA8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140T8S5A0A140T8S5_MOUSE
Chromosome alignment-maintaining ph...
Champ1 D8Ertd457e, mCG_60366
802Annotation score:

Sequence cautioni

The sequence BAC98254 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170C → S in BAC98254 (PubMed:14621295).Curated1
Sequence conflicti244P → T in BAC98254 (PubMed:14621295).Curated1
Sequence conflicti335P → H in BAE21972 (PubMed:16141072).Curated1
Sequence conflicti549F → L in BAC98254 (PubMed:14621295).Curated1
Sequence conflicti661M → K in BAE21972 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129444 mRNA Translation: BAC98254.1 Different initiation.
AK132164 mRNA Translation: BAE21006.1
AK133990 mRNA Translation: BAE21972.1
AK170835 mRNA Translation: BAE42063.1
BC028991 mRNA Translation: AAH28991.1
CCDSiCCDS22116.1
RefSeqiNP_862902.1, NM_181854.2
XP_006508742.1, XM_006508679.2
UniGeneiMm.289346
Mm.412576

Genome annotation databases

EnsembliENSMUST00000051870; ENSMUSP00000057270; ENSMUSG00000047710
ENSMUST00000128557; ENSMUSP00000120117; ENSMUSG00000047710
GeneIDi101994
KEGGimmu:101994
UCSCiuc009kyp.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129444 mRNA Translation: BAC98254.1 Different initiation.
AK132164 mRNA Translation: BAE21006.1
AK133990 mRNA Translation: BAE21972.1
AK170835 mRNA Translation: BAE42063.1
BC028991 mRNA Translation: AAH28991.1
CCDSiCCDS22116.1
RefSeqiNP_862902.1, NM_181854.2
XP_006508742.1, XM_006508679.2
UniGeneiMm.289346
Mm.412576

3D structure databases

ProteinModelPortaliQ8K327
SMRiQ8K327
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221774, 2 interactors
IntActiQ8K327, 2 interactors
MINTiQ8K327
STRINGi10090.ENSMUSP00000057270

PTM databases

iPTMnetiQ8K327
PhosphoSitePlusiQ8K327

Proteomic databases

EPDiQ8K327
MaxQBiQ8K327
PaxDbiQ8K327
PeptideAtlasiQ8K327
PRIDEiQ8K327

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051870; ENSMUSP00000057270; ENSMUSG00000047710
ENSMUST00000128557; ENSMUSP00000120117; ENSMUSG00000047710
GeneIDi101994
KEGGimmu:101994
UCSCiuc009kyp.2 mouse

Organism-specific databases

CTDi283489
MGIiMGI:1196398 Champ1
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IFC8 Eukaryota
ENOG410XXII LUCA
GeneTreeiENSGT00730000111351
HOGENOMiHOG000065731
HOVERGENiHBG062352
InParanoidiQ8K327
KOiK22593
OMAiTSKMYSN
OrthoDBiEOG091G02UO
PhylomeDBiQ8K327
TreeFamiTF350859

Miscellaneous databases

PROiPR:Q8K327
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047710 Expressed in 251 organ(s), highest expression level in embryo
CleanExiMM_ZFP828
ExpressionAtlasiQ8K327 baseline and differential
GenevisibleiQ8K327 MM

Family and domain databases

InterProiView protein in InterPro
IPR039330 CAMP
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR37354 PTHR37354, 1 hit
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHAP1_MOUSE
AccessioniPrimary (citable) accession number: Q8K327
Secondary accession number(s): Q3UZ85, Q6ZPI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again