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Protein

General transcription factor IIH subunit 5

Gene

Gtf2h5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Necessary for the stability of the TFIIH complex and for the presence of normal levels of TFIIH in the cell.By similarity

GO - Molecular functioni

  • rDNA binding Source: MGI

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 5
Alternative name(s):
General transcription factor IIH polypeptide 5
TFB5 ortholog
TFIIH basal transcription factor complex TTD-A subunit
Gene namesi
Name:Gtf2h5
Synonyms:D17Wsu155e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107227 Gtf2h5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192571 – 71General transcription factor IIH subunit 5Add BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K2X8
PaxDbiQ8K2X8
PeptideAtlasiQ8K2X8
PRIDEiQ8K2X8

PTM databases

iPTMnetiQ8K2X8
PhosphoSitePlusiQ8K2X8

Expressioni

Gene expression databases

BgeeiENSMUSG00000034345 Expressed in 257 organ(s), highest expression level in blastocyst
CleanExiMM_GTF2H5
GenevisibleiQ8K2X8 MM

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.By similarity

Protein-protein interaction databases

BioGridi211495, 1 interactor
STRINGi10090.ENSMUSP00000040355

Structurei

3D structure databases

ProteinModelPortaliQ8K2X8
SMRiQ8K2X8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB5 family.Curated

Phylogenomic databases

eggNOGiKOG3451 Eukaryota
ENOG41121VZ LUCA
GeneTreeiENSGT00390000004028
HOGENOMiHOG000199698
HOVERGENiHBG055393
InParanoidiQ8K2X8
KOiK10845
OMAiIMENMNP
OrthoDBiEOG091G1339
PhylomeDBiQ8K2X8
TreeFamiTF319487

Family and domain databases

Gene3Di3.30.70.1220, 1 hit
InterProiView protein in InterPro
IPR035935 TFB5-like_sf
IPR009400 TFIIH_TTDA/Tfb5
PANTHERiPTHR28580 PTHR28580, 1 hit
PfamiView protein in Pfam
PF06331 Tfb5, 1 hit
SMARTiView protein in SMART
SM01395 Tbf5, 1 hit
SUPFAMiSSF142897 SSF142897, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K2X8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVNVLKGVLI ECDPAMKQFL LYLDEANALG KKFIIQDIDD THVFVIAELV
60 70
NVLQERVGEL MDQNAFSLTQ K
Length:71
Mass (Da):8,037
Last modified:October 1, 2002 - v1
Checksum:i940B4D3C9BED9A8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7G → R in BAC25398 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003547 mRNA Translation: BAC25038.1
AK003579 mRNA Translation: BAC25043.1
AK011281 mRNA Translation: BAC25328.1
AK012257 mRNA Translation: BAC25362.1
AK013225 mRNA Translation: BAC25398.1
BC029238 mRNA Translation: AAH29238.1
CCDSiCCDS37423.1
RefSeqiNP_852057.2, NM_181392.3
XP_006523302.1, XM_006523239.3
XP_006523303.1, XM_006523240.3
UniGeneiMm.303441

Genome annotation databases

EnsembliENSMUST00000039487; ENSMUSP00000040355; ENSMUSG00000034345
ENSMUST00000100955; ENSMUSP00000098515; ENSMUSG00000034345
GeneIDi66467
KEGGimmu:66467
UCSCiuc008aga.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003547 mRNA Translation: BAC25038.1
AK003579 mRNA Translation: BAC25043.1
AK011281 mRNA Translation: BAC25328.1
AK012257 mRNA Translation: BAC25362.1
AK013225 mRNA Translation: BAC25398.1
BC029238 mRNA Translation: AAH29238.1
CCDSiCCDS37423.1
RefSeqiNP_852057.2, NM_181392.3
XP_006523302.1, XM_006523239.3
XP_006523303.1, XM_006523240.3
UniGeneiMm.303441

3D structure databases

ProteinModelPortaliQ8K2X8
SMRiQ8K2X8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211495, 1 interactor
STRINGi10090.ENSMUSP00000040355

PTM databases

iPTMnetiQ8K2X8
PhosphoSitePlusiQ8K2X8

Proteomic databases

MaxQBiQ8K2X8
PaxDbiQ8K2X8
PeptideAtlasiQ8K2X8
PRIDEiQ8K2X8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039487; ENSMUSP00000040355; ENSMUSG00000034345
ENSMUST00000100955; ENSMUSP00000098515; ENSMUSG00000034345
GeneIDi66467
KEGGimmu:66467
UCSCiuc008aga.2 mouse

Organism-specific databases

CTDi404672
MGIiMGI:107227 Gtf2h5

Phylogenomic databases

eggNOGiKOG3451 Eukaryota
ENOG41121VZ LUCA
GeneTreeiENSGT00390000004028
HOGENOMiHOG000199698
HOVERGENiHBG055393
InParanoidiQ8K2X8
KOiK10845
OMAiIMENMNP
OrthoDBiEOG091G1339
PhylomeDBiQ8K2X8
TreeFamiTF319487

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Miscellaneous databases

ChiTaRSiGtf2h5 mouse
PROiPR:Q8K2X8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034345 Expressed in 257 organ(s), highest expression level in blastocyst
CleanExiMM_GTF2H5
GenevisibleiQ8K2X8 MM

Family and domain databases

Gene3Di3.30.70.1220, 1 hit
InterProiView protein in InterPro
IPR035935 TFB5-like_sf
IPR009400 TFIIH_TTDA/Tfb5
PANTHERiPTHR28580 PTHR28580, 1 hit
PfamiView protein in Pfam
PF06331 Tfb5, 1 hit
SMARTiView protein in SMART
SM01395 Tbf5, 1 hit
SUPFAMiSSF142897 SSF142897, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTF2H5_MOUSE
AccessioniPrimary (citable) accession number: Q8K2X8
Secondary accession number(s): Q8BT75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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