UniProtKB - Q8K2P7 (S38A1_MOUSE)
Protein
Sodium-coupled neutral amino acid transporter 1
Gene
Slc38a1
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.1 Publication
Activity regulationi
Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB).By similarity
Kineticsi
Decrease in pH from 8.0 to 7.0 results in decreased affinity for L-glutamine.
- KM=2400 µM for L-glutamine (at pH 7.0)1 Publication
- KM=890 µM for L-glutamine (at pH 7.5)1 Publication
- KM=540 µM for L-glutamine (at pH 8.0)1 Publication
GO - Molecular functioni
- amino acid transmembrane transporter activity Source: GO_Central
- L-amino acid transmembrane transporter activity Source: MGI
- L-glutamine transmembrane transporter activity Source: MGI
- neutral amino acid:sodium symporter activity Source: MGI
GO - Biological processi
- amino acid transmembrane transport Source: GO_Central
- female pregnancy Source: Ensembl
- glutamine transport Source: MGI
- L-alpha-amino acid transmembrane transport Source: MGI
- neutral amino acid transport Source: MGI
Keywordsi
Biological process | Amino-acid transport, Ion transport, Sodium transport, Symport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
Reactomei | R-MMU-210455, Astrocytic Glutamate-Glutamine Uptake And Metabolism R-MMU-352230, Amino acid transport across the plasma membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium-coupled neutral amino acid transporter 1Alternative name(s): Amino acid transporter A1 MNat2 N-system amino acid transporter 2 Solute carrier family 38 member 1 System A amino acid transporter 1 System N amino acid transporter 1 |
Gene namesi | Name:Slc38a1 Synonyms:Nat2, Snat1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2145895, Slc38a1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain.By similarity
Plasma Membrane
- basolateral plasma membrane Source: ARUK-UCL
- external side of apical plasma membrane Source: MGI
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: MGI
Other locations
- axon Source: MGI
- membrane Source: MGI
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 74 | CytoplasmicSequence analysisAdd BLAST | 74 | |
Transmembranei | 75 – 97 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 98 – 112 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 113 – 133 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 134 – 148 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 149 – 169 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 170 – 188 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 189 – 211 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 212 – 216 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 217 – 237 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 238 – 273 | ExtracellularSequence analysisAdd BLAST | 36 | |
Transmembranei | 274 – 294 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 295 – 310 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 311 – 331 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 332 – 348 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 349 – 369 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 370 – 391 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 392 – 412 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 413 – 414 | ExtracellularSequence analysis | 2 | |
Transmembranei | 415 – 435 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 436 – 450 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 451 – 471 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 472 – 485 | ExtracellularSequence analysisAdd BLAST | 14 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000310476 | 1 – 485 | Sodium-coupled neutral amino acid transporter 1Add BLAST | 485 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Modified residuei | 11 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 25 | PhosphoserineBy similarity | 1 | |
Modified residuei | 28 | PhosphoserineBy similarity | 1 | |
Modified residuei | 49 | PhosphoserineBy similarity | 1 | |
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 54 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 56 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 245 ↔ 262 | PROSITE-ProRule annotation | ||
Glycosylationi | 251 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 257 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q8K2P7 |
PaxDbi | Q8K2P7 |
PeptideAtlasi | Q8K2P7 |
PRIDEi | Q8K2P7 |
PTM databases
GlyGeni | Q8K2P7, 2 sites |
iPTMneti | Q8K2P7 |
PhosphoSitePlusi | Q8K2P7 |
SwissPalmi | Q8K2P7 |
Expressioni
Tissue specificityi
Specifically expressed in brain and retina (at protein level). Also detected in spleen, small intestine and lung.1 Publication
Inductioni
Up-regulated by BDNF.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000023169, Expressed in pituitary gland and 293 other tissues |
Genevisiblei | Q8K2P7, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 222905, 1 interactor |
STRINGi | 10090.ENSMUSP00000097833 |
Miscellaneous databases
RNActi | Q8K2P7, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid/polyamine transporter 2 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1305, Eukaryota |
GeneTreei | ENSGT00940000160716 |
HOGENOMi | CLU_009020_0_2_1 |
InParanoidi | Q8K2P7 |
OMAi | LVIYDWV |
OrthoDBi | 697331at2759 |
PhylomeDBi | Q8K2P7 |
TreeFami | TF328787 |
Family and domain databases
InterProi | View protein in InterPro IPR013057, AA_transpt_TM |
Pfami | View protein in Pfam PF01490, Aa_trans, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8K2P7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR
60 70 80 90 100
RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG
110 120 130 140 150
ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI
160 170 180 190 200
FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEDAFSAW YVDGRVLVVM
210 220 230 240 250
VTFGIILPLC LLKNLGYLGY TSGFSLSCMM FFLIVVIYKK FQTPCMSVEQ
260 270 280 290 300
NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR
310 320 330 340 350
SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL
360 370 380 390 400
ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLI
410 420 430 440 450
IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKGTQR
460 470 480
IWAALFLGLG VLFSLISIPL VIYDWACSSG TDEGH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 48 | E → G in BAE38067 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 244 | P → H in BAC33064 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 354 | V → A in AAG43433 (PubMed:11325958).Curated | 1 | |
Sequence conflicti | 360 | V → A in AAG43433 (PubMed:11325958).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029305 | 67 – 72 | IPGTTS → VSIICM in isoform 3. 1 Publication | 6 | |
Alternative sequenceiVSP_029306 | 73 – 485 | Missing in isoform 3. 1 PublicationAdd BLAST | 413 | |
Alternative sequenceiVSP_029307 | 333 – 344 | EKVQS…LHKYQ → GKLLPLAMPICT in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_029308 | 345 – 485 | Missing in isoform 2. 1 PublicationAdd BLAST | 141 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF184240 mRNA Translation: AAG43433.2 AK029189 mRNA Translation: BAC26341.1 AK034130 mRNA Translation: BAC28597.1 AK035098 mRNA Translation: BAC28944.1 AK047459 mRNA Translation: BAC33064.1 AK049294 mRNA Translation: BAC33663.1 AK050914 mRNA Translation: BAC34457.1 AK081724 mRNA Translation: BAC38310.1 AK162612 mRNA Translation: BAE36989.1 AK163914 mRNA Translation: BAE37532.1 AK165188 mRNA Translation: BAE38067.1 BC030378 mRNA Translation: AAH30378.1 BC066815 mRNA Translation: AAH66815.1 |
CCDSi | CCDS27777.1 [Q8K2P7-1] |
RefSeqi | NP_001159928.1, NM_001166456.1 [Q8K2P7-1] NP_001159930.1, NM_001166458.1 [Q8K2P7-1] NP_598847.2, NM_134086.4 [Q8K2P7-1] XP_006520298.1, XM_006520235.3 [Q8K2P7-1] |
Genome annotation databases
Ensembli | ENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169 [Q8K2P7-1] ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169 [Q8K2P7-1] ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169 [Q8K2P7-1] ENSMUST00000230767; ENSMUSP00000155160; ENSMUSG00000023169 [Q8K2P7-3] |
GeneIDi | 105727 |
KEGGi | mmu:105727 |
UCSCi | uc007xkh.2, mouse [Q8K2P7-1] uc007xkk.2, mouse [Q8K2P7-2] uc007xkl.2, mouse [Q8K2P7-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF184240 mRNA Translation: AAG43433.2 AK029189 mRNA Translation: BAC26341.1 AK034130 mRNA Translation: BAC28597.1 AK035098 mRNA Translation: BAC28944.1 AK047459 mRNA Translation: BAC33064.1 AK049294 mRNA Translation: BAC33663.1 AK050914 mRNA Translation: BAC34457.1 AK081724 mRNA Translation: BAC38310.1 AK162612 mRNA Translation: BAE36989.1 AK163914 mRNA Translation: BAE37532.1 AK165188 mRNA Translation: BAE38067.1 BC030378 mRNA Translation: AAH30378.1 BC066815 mRNA Translation: AAH66815.1 |
CCDSi | CCDS27777.1 [Q8K2P7-1] |
RefSeqi | NP_001159928.1, NM_001166456.1 [Q8K2P7-1] NP_001159930.1, NM_001166458.1 [Q8K2P7-1] NP_598847.2, NM_134086.4 [Q8K2P7-1] XP_006520298.1, XM_006520235.3 [Q8K2P7-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 222905, 1 interactor |
STRINGi | 10090.ENSMUSP00000097833 |
PTM databases
GlyGeni | Q8K2P7, 2 sites |
iPTMneti | Q8K2P7 |
PhosphoSitePlusi | Q8K2P7 |
SwissPalmi | Q8K2P7 |
Proteomic databases
EPDi | Q8K2P7 |
PaxDbi | Q8K2P7 |
PeptideAtlasi | Q8K2P7 |
PRIDEi | Q8K2P7 |
Protocols and materials databases
ABCDi | Q8K2P7, 2 sequenced antibodies |
Antibodypediai | 7400, 192 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169 [Q8K2P7-1] ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169 [Q8K2P7-1] ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169 [Q8K2P7-1] ENSMUST00000230767; ENSMUSP00000155160; ENSMUSG00000023169 [Q8K2P7-3] |
GeneIDi | 105727 |
KEGGi | mmu:105727 |
UCSCi | uc007xkh.2, mouse [Q8K2P7-1] uc007xkk.2, mouse [Q8K2P7-2] uc007xkl.2, mouse [Q8K2P7-3] |
Organism-specific databases
CTDi | 81539 |
MGIi | MGI:2145895, Slc38a1 |
Phylogenomic databases
eggNOGi | KOG1305, Eukaryota |
GeneTreei | ENSGT00940000160716 |
HOGENOMi | CLU_009020_0_2_1 |
InParanoidi | Q8K2P7 |
OMAi | LVIYDWV |
OrthoDBi | 697331at2759 |
PhylomeDBi | Q8K2P7 |
TreeFami | TF328787 |
Enzyme and pathway databases
Reactomei | R-MMU-210455, Astrocytic Glutamate-Glutamine Uptake And Metabolism R-MMU-352230, Amino acid transport across the plasma membrane |
Miscellaneous databases
BioGRID-ORCSi | 105727, 1 hit in 20 CRISPR screens |
ChiTaRSi | Slc38a1, mouse |
PROi | PR:Q8K2P7 |
RNActi | Q8K2P7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023169, Expressed in pituitary gland and 293 other tissues |
Genevisiblei | Q8K2P7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR013057, AA_transpt_TM |
Pfami | View protein in Pfam PF01490, Aa_trans, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S38A1_MOUSE | |
Accessioni | Q8K2P7Primary (citable) accession number: Q8K2P7 Secondary accession number(s): Q3TNM1 Q99PR1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families