UniProtKB - Q8K2H1 (PPHLN_MOUSE)

BLAST

>sp|Q8K2H1|PPHLN_MOUSE Periphilin-1 OS=Mus musculus OX=10090 GN=Pphln1 PE=1 SV=1
MWSEGRYDYDRLPRERVPPRSHPSDGYHRVVNVVPKRPPLLDKRPPLLDKRPPLLARPDE
GGYSRYYSHVDCRVCDEGRSFSHDRRSGPSHSGDESGYRWLRDDHSTSRQPDYRDMRDGF
RRKSFYSSHYSRDRSPHKRDAPFFRESPVGRKDSPHSRSGSSVSSRSYSPERSRTHSFHQ
SQHRKSSRVGASYKRQNEAIRGRGKERSIQSVKTSRDASPSSSSAVASSKALDKPSRLTE
KELAEAESKWANETLEKSDESNLAEMNEFEAGSTAPLFIDQTEEPESNTVDGTELYEDSQ
LSNRSKAIASKTKEIEQVYRQDCETFGMVVKMLIEKDPSLEKSVQFALRQNLHEIGERCV
EELKRFITEYDNSARDFGDPF

Align

Format
Add to basket Added to basket
History
Entry version 107 (07 Nov 2018)
Sequence version 1 (01 Oct 2002)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

Periphilin-1

Gene

Pphln1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin. Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex. May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation.By similarity

GO - Biological processⁱ

keratinization Source: UniProtKB-KW
negative regulation of transcription, DNA-templated Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Repressor
Biological process	Keratinization, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Periphilin-1By similarity
Gene namesⁱ	Name:Pphln1Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:1917029 Pphln1

Keywords - Cellular componentⁱ

Chromosome, Cytoplasm, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Embryonic lethality. Embryos die during early embryogenesis.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000058538	1 – 381	Periphilin-1Add BLAST		381

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	123	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	124	PhosphoserineBy similarity	1
Modified residueⁱ	128	PhosphoserineBy similarity	1
Modified residueⁱ	147	PhosphoserineBy similarity	1
Modified residueⁱ	154	PhosphoserineBy similarity	1
Modified residueⁱ	181	PhosphoserineBy similarity	1
Cross-linkⁱ	194	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	211	PhosphoserineBy similarity	1
Cross-linkⁱ	213	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	215	PhosphoserineBy similarity	1
Modified residueⁱ	219	PhosphoserineCombined sources	1
Cross-linkⁱ	241	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	249	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	249	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residueⁱ	339	PhosphoserineBy similarity	1
Cross-linkⁱ	342	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q8K2H1
PeptideAtlas More...PeptideAtlasⁱ	Q8K2H1
PRIDEⁱ	Q8K2H1

PTM databases

iPTMnetⁱ	Q8K2H1
PhosphoSitePlusⁱ	Q8K2H1

Miscellaneous databases

PMAP-CutDBⁱ	Q8K2H1

PMAP-CutDBⁱ

Q8K2H1

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed. Strong expression in the developing somites and limbs, the embryonic nervous system and the adult brain.1 Publication

Gene expression databases

CleanExⁱ	MM_PPHLN1

CleanExⁱ

MM_PPHLN1

Interactionⁱ

Subunit structureⁱ

Homodimer. Component of the HUSH complex; at least composed of FAM208A/TASOR, PPHLN1 and MPHOSPH8. Interacts with SIN3A and HDAC1. Interacts with PPL.By similarity

Protein-protein interaction databases

BioGridⁱ	230202, 3 interactors
IntActⁱ	Q8K2H1, 1 interactor
STRINGⁱ	10090.ENSMUSP00000042762

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	117 – 123	Nuclear localization signalBy similarity		7

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	154 – 229	Ser-richAdd BLAST		76

Phylogenomic databases

eggNOGⁱ	ENOG410IFSB Eukaryota ENOG4111JYA LUCA
HOGENOMⁱ	HOG000074159
HOVERGENⁱ	HBG053653
InParanoidⁱ	Q8K2H1
KEGG Orthology (KO) More...KOⁱ	K21872
PhylomeDBⁱ	Q8K2H1

Family and domain databases

InterProⁱ	View protein in InterPro IPR028851 Pphln1
PANTHERⁱ	PTHR15836 PTHR15836, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q8K2H1-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWSEGRYDYD RLPRERVPPR SHPSDGYHRV VNVVPKRPPL LDKRPPLLDK 
        60         70         80         90        100
RPPLLARPDE GGYSRYYSHV DCRVCDEGRS FSHDRRSGPS HSGDESGYRW 
       110        120        130        140        150
LRDDHSTSRQ PDYRDMRDGF RRKSFYSSHY SRDRSPHKRD APFFRESPVG 
       160        170        180        190        200
RKDSPHSRSG SSVSSRSYSP ERSRTHSFHQ SQHRKSSRVG ASYKRQNEAI 
       210        220        230        240        250
RGRGKERSIQ SVKTSRDASP SSSSAVASSK ALDKPSRLTE KELAEAESKW 
       260        270        280        290        300
ANETLEKSDE SNLAEMNEFE AGSTAPLFID QTEEPESNTV DGTELYEDSQ 
       310        320        330        340        350
LSNRSKAIAS KTKEIEQVYR QDCETFGMVV KMLIEKDPSL EKSVQFALRQ 
       360        370        380 
NLHEIGERCV EELKRFITEY DNSARDFGDP F

Note: No experimental confirmation available.

Length:381

Mass (Da):43,835

Last modified:October 1, 2002 - v1

Checksum:ⁱ90C3AC70BA644D77

Isoform 2 (identifier: Q8K2H1-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
25-93: Missing.

« Hide

        10         20         30         40         50
MWSEGRYDYD RLPRERVPPR SHPSDESGYR WLRDDHSTSR QPDYRDMRDG 
        60         70         80         90        100
FRRKSFYSSH YSRDRSPHKR DAPFFRESPV GRKDSPHSRS GSSVSSRSYS 
       110        120        130        140        150
PERSRTHSFH QSQHRKSSRV GASYKRQNEA IRGRGKERSI QSVKTSRDAS 
       160        170        180        190        200
PSSSSAVASS KALDKPSRLT EKELAEAESK WANETLEKSD ESNLAEMNEF 
       210        220        230        240        250
EAGSTAPLFI DQTEEPESNT VDGTELYEDS QLSNRSKAIA SKTKEIEQVY 
       260        270        280        290        300
RQDCETFGMV VKMLIEKDPS LEKSVQFALR QNLHEIGERC VEELKRFITE 
       310 
YDNSARDFGD PF

Note: No experimental confirmation available.

Show »

Length:312

Mass (Da):35,985

Checksum:ⁱ0FCE5AA9928B753E

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

G3X959	G3X959_MOUSE	Periphilin 1, isoform CRA_a Periphilin 1, isoform CRA_a (Periphilin-1)	Pphln1 mCG_114279	381	Annotation score:
Q3UBL8	Q3UBL8_MOUSE	Periphilin 1, isoform CRA_d Periphilin 1, isoform CRA_d (Periphilin-1)	Pphln1 mCG_114279	312	Annotation score:
G3UWD4	G3UWD4_MOUSE	Periphilin 1, isoform CRA_b Periphilin 1, isoform CRA_b (Periphilin-1)	Pphln1 mCG_114279	293	Annotation score:
A0A2R8W6Q1	A0A2R8W6Q1_MOUSE	Periphilin-1 Periphilin-1	Pphln1	125	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	375	R → Q in BAC38270 (PubMed:16141072).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_009908	25 – 93	Missing in isoform 2. 1 PublicationAdd BLAST		69

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK081606 mRNA Translation: BAC38270.1 BC031486 mRNA Translation: AAH31486.3
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27767.1 [Q8K2H1-1] CCDS27768.1 [Q8K2H1-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_780572.1, NM_175363.4 XP_006520920.1, XM_006520857.1
UniGeneⁱ	Mm.74208

Genome annotation databases

GeneIDⁱ	223828
KEGGⁱ	mmu:223828

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8K2H1	Periphilin 1		RAT		410	UniRef90_Q8K2H1
UPI000A308EB4		MUSPA		381
Periphilin 1		RAT		406
UPI0003D07D26		RAT		468
UPI0000182A4E		RAT		380
+1
Q8K2H1-2	Periphilin 1, isoform CRA_d		MOUSE		312	UniRef90_Q8K2H1-2
UPI000A316A85		MUSCR		342
UPI000B4EB167		MERUN		331
UPI000454ABC5		CRIGR		313
UPI000A30752D		MUSPA		312
+9

Protein	Similar proteins		Species	Score	Length	Source
Q8K2H1	Periphilin 1		RAT		410	UniRef50_Q8K2H1
Uncharacterized protein		RHIBE		427
Periphilin-1		HETGA		403
Uncharacterized protein		CAVPO		269
Periphilin 1		GORGO		380
+527
Q8K2H1-2	Periphilin 1, isoform CRA_d		MOUSE		312	UniRef50_Q8K2H1-2
Periphilin-1		CRIGR		413
UPI000A316A85		MUSCR		342
UPI000B4EB167		MERUN		331
UPI000A30752D		MUSPA		312
+14

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK081606 mRNA Translation: BAC38270.1 BC031486 mRNA Translation: AAH31486.3
CCDSⁱ	CCDS27767.1 [Q8K2H1-1] CCDS27768.1 [Q8K2H1-2]
RefSeqⁱ	NP_780572.1, NM_175363.4 XP_006520920.1, XM_006520857.1
UniGeneⁱ	Mm.74208

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	230202, 3 interactors
IntActⁱ	Q8K2H1, 1 interactor
STRINGⁱ	10090.ENSMUSP00000042762

PTM databases

iPTMnetⁱ	Q8K2H1
PhosphoSitePlusⁱ	Q8K2H1

Proteomic databases

PaxDbⁱ	Q8K2H1
PeptideAtlasⁱ	Q8K2H1
PRIDEⁱ	Q8K2H1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	223828
KEGGⁱ	mmu:223828

Organism-specific databases

CTDⁱ	51535
MGIⁱ	MGI:1917029 Pphln1

Phylogenomic databases

eggNOGⁱ	ENOG410IFSB Eukaryota ENOG4111JYA LUCA
HOGENOMⁱ	HOG000074159
HOVERGENⁱ	HBG053653
InParanoidⁱ	Q8K2H1
KOⁱ	K21872
PhylomeDBⁱ	Q8K2H1

Miscellaneous databases

ChiTaRSⁱ	Pphln1 mouse
PMAP-CutDBⁱ	Q8K2H1
Protein Ontology More...PROⁱ	PR:Q8K2H1
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_PPHLN1

CleanExⁱ

MM_PPHLN1

Family and domain databases

InterProⁱ	View protein in InterPro IPR028851 Pphln1
PANTHERⁱ	PTHR15836 PTHR15836, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PPHLN_MOUSE
Accessionⁱ	Q8K2H1Primary (citable) accession number: Q8K2H1 Secondary accession number(s): Q8BUZ6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
	Last sequence update:	October 1, 2002
	Last modified:	November 7, 2018
	This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8K2H1 (PPHLN_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Periphilin-1

Pphln1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Golgi apparatus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Compositional bias

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ