UniProtKB - Q8K2F0 (BRD3_MOUSE)
Bromodomain-containing protein 3
Brd3
Functioni
Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:21536911).
Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (PubMed:21536911).
1 PublicationGO - Molecular functioni
- chromatin binding Source: MGI
- lysine-acetylated histone binding Source: UniProtKB
GO - Biological processi
- chromatin remodeling Source: GO_Central
- regulation of transcription, DNA-templated Source: GO_Central
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Chromatin regulator |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Bromodomain-containing protein 3Alternative name(s): Bromodomain-containing FSH-like protein FSRG2 |
Gene namesi | Name:Brd3 Synonyms:Fsrg2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914632, Brd3 |
VEuPathDBi | HostDB:ENSMUSG00000026918 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Detected on chromatin.1 Publication
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211182 | 1 – 726 | Bromodomain-containing protein 3Add BLAST | 726 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 262 | PhosphoserineCombined sources | 1 | |
Modified residuei | 280 | PhosphoserineBy similarity | 1 | |
Cross-linki | 413 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 564 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8K2F0 |
jPOSTi | Q8K2F0 |
MaxQBi | Q8K2F0 |
PaxDbi | Q8K2F0 |
PRIDEi | Q8K2F0 |
ProteomicsDBi | 273760 [Q8K2F0-1] 273761 [Q8K2F0-2] |
PTM databases
iPTMneti | Q8K2F0 |
PhosphoSitePlusi | Q8K2F0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026918, Expressed in undifferentiated genital tubercle and 306 other tissues |
ExpressionAtlasi | Q8K2F0, baseline and differential |
Genevisiblei | Q8K2F0, MM |
Interactioni
Subunit structurei
Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro) (PubMed:21536911).
Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315' (PubMed:21555453).
Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues (PubMed:21555453).
2 PublicationsGO - Molecular functioni
- lysine-acetylated histone binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 212148, 3 interactors |
STRINGi | 10090.ENSMUSP00000028282 |
Miscellaneous databases
RNActi | Q8K2F0, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8K2F0 |
SMRi | Q8K2F0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8K2F0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 122 | Bromo 1PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 325 – 397 | Bromo 2PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 563 – 645 | NETPROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 34 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 77 – 79 | Acetylated histone H3 bindingBy similarity | 3 | |
Regioni | 147 – 172 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 236 – 308 | DisorderedSequence analysisAdd BLAST | 73 | |
Regioni | 418 – 576 | DisorderedSequence analysisAdd BLAST | 159 | |
Regioni | 638 – 726 | DisorderedSequence analysisAdd BLAST | 89 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 452 – 482 | Sequence analysisAdd BLAST | 31 | |
Coiled coili | 646 – 689 | Sequence analysisAdd BLAST | 44 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 28 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 246 – 265 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 272 – 308 | Basic and acidic residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 434 – 448 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 451 – 468 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 497 – 525 | Basic and acidic residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 535 – 557 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 653 – 674 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 696 – 726 | Polar residuesSequence analysisAdd BLAST | 31 |
Domaini
Keywords - Domaini
Bromodomain, Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG1474, Eukaryota |
GeneTreei | ENSGT00940000153385 |
InParanoidi | Q8K2F0 |
OMAi | CYAFNPD |
TreeFami | TF317345 |
Family and domain databases
CDDi | cd05497, Bromo_Brdt_I_like, 1 hit cd05498, Bromo_Brdt_II_like, 1 hit |
Gene3Di | 1.20.1270.220, 1 hit 1.20.920.10, 2 hits |
IDEALi | IID50153 |
InterProi | View protein in InterPro IPR043508, Bromo_Brdt_I IPR043509, Bromo_Brdt_II IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR018359, Bromodomain_CS IPR027353, NET_dom IPR038336, NET_sf |
Pfami | View protein in Pfam PF17035, BET, 1 hit PF00439, Bromodomain, 2 hits |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 2 hits |
SUPFAMi | SSF47370, SSF47370, 2 hits |
PROSITEi | View protein in PROSITE PS00633, BROMODOMAIN_1, 2 hits PS50014, BROMODOMAIN_2, 2 hits PS51525, NET, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSTTAAAPTG IPAVPGPVNP PPPEVSNPSK PGRKTNQLQY MQNVVVKTLW
60 70 80 90 100
KHQFAWPFYQ PVDAIKLNLP DYHKIIKNPM DMGTIKKRLE NNYYWSASEC
110 120 130 140 150
MQDFNTMFTN CYIYNKPTDD IVLMAQALEK IFLQKVAQMP QEEVELLPPA
160 170 180 190 200
PKGKGRKPAA GAQNAGSQQV AAVSSVSPAT PFQNIPPTVS QTPVIAATPV
210 220 230 240 250
PTITANVTSV PVPPPAAPPP PATPIVPVVP PTPPVVKKKG VKRKADTTTP
260 270 280 290 300
TTSAITASRS ESPPPLSEPK QAKVVARRES GGRPIKPPKK DLEDGEVPQH
310 320 330 340 350
AGKKGKLSEH LRHCDSILRE MLSKKHAAYA WPFYKPVDAE ALELHDYHDI
360 370 380 390 400
IKHPMDLSTV KRKMDSREYP DAQGFAADIR LMFSNCYKYN PPDHEVVAMA
410 420 430 440 450
RKLQDVFEMR FAKMPDEPME APALPAPTAP IVSKGAESSR SSEESSSDSG
460 470 480 490 500
SSDSEEERAT RLAELQEQLK AVHEQLAALS QAPVNKPKKK KEKKEKEKKK
510 520 530 540 550
KDKDKDKEKE KHKAKSEEEK KAKAAPAAKQ AQQKKAPTKK ANSTTTASRQ
560 570 580 590 600
LKKGGKQASA SYDSEEEEEG LPMSYDEKRQ LSLDINRLPG EKLGRVVHII
610 620 630 640 650
QSREPSLRDS NPDEIEIDFE TLKPTTLREL ERYVKSCLQK KQRKPLSTSG
660 670 680 690 700
KKQAAKSKEE LAQEKKKELE KRLQDVSGQL NSKKPTKKEK SGSAPSGGPS
710 720
RLSSSSSSES ASSSSSGSSS DSSDSE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AKA9 | A2AKA9_MOUSE | Bromodomain-containing protein 3 | Brd3 | 682 | Annotation score: | ||
A2AKB0 | A2AKB0_MOUSE | Bromodomain-containing protein 3 | Brd3 | 90 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 174 | S → F in BAC36359 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 216 | A → T in AAH31536 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 477 | A → D in AAF78072 (Ref. 1) Curated | 1 | |
Sequence conflicti | 659 – 660 | EE → VV in AAF78072 (Ref. 1) Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010249 | 468 | Q → QTGCGAFQDQLLNVSSVQ in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF269193 mRNA Translation: AAF78072.1 BC031536 mRNA Translation: AAH31536.1 AK037435 mRNA Translation: BAC29806.1 AK076472 mRNA Translation: BAC36359.1 |
CCDSi | CCDS15828.1 [Q8K2F0-1] CCDS50546.1 [Q8K2F0-2] |
RefSeqi | NP_001107045.1, NM_001113573.1 [Q8K2F0-1] NP_001107046.1, NM_001113574.1 [Q8K2F0-2] NP_075825.3, NM_023336.4 [Q8K2F0-1] |
Genome annotation databases
Ensembli | ENSMUST00000028282; ENSMUSP00000028282; ENSMUSG00000026918 [Q8K2F0-1] ENSMUST00000077737; ENSMUSP00000076918; ENSMUSG00000026918 [Q8K2F0-1] ENSMUST00000113941; ENSMUSP00000109574; ENSMUSG00000026918 [Q8K2F0-2] ENSMUST00000164296; ENSMUSP00000128812; ENSMUSG00000026918 [Q8K2F0-1] |
GeneIDi | 67382 |
KEGGi | mmu:67382 |
UCSCi | uc008ixm.2, mouse [Q8K2F0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF269193 mRNA Translation: AAF78072.1 BC031536 mRNA Translation: AAH31536.1 AK037435 mRNA Translation: BAC29806.1 AK076472 mRNA Translation: BAC36359.1 |
CCDSi | CCDS15828.1 [Q8K2F0-1] CCDS50546.1 [Q8K2F0-2] |
RefSeqi | NP_001107045.1, NM_001113573.1 [Q8K2F0-1] NP_001107046.1, NM_001113574.1 [Q8K2F0-2] NP_075825.3, NM_023336.4 [Q8K2F0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2L5E | NMR | - | A | 24-146 | [»] | |
AlphaFoldDBi | Q8K2F0 | |||||
SMRi | Q8K2F0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 212148, 3 interactors |
STRINGi | 10090.ENSMUSP00000028282 |
PTM databases
iPTMneti | Q8K2F0 |
PhosphoSitePlusi | Q8K2F0 |
Proteomic databases
EPDi | Q8K2F0 |
jPOSTi | Q8K2F0 |
MaxQBi | Q8K2F0 |
PaxDbi | Q8K2F0 |
PRIDEi | Q8K2F0 |
ProteomicsDBi | 273760 [Q8K2F0-1] 273761 [Q8K2F0-2] |
Protocols and materials databases
Antibodypediai | 31966, 387 antibodies from 34 providers |
DNASUi | 67382 |
Genome annotation databases
Ensembli | ENSMUST00000028282; ENSMUSP00000028282; ENSMUSG00000026918 [Q8K2F0-1] ENSMUST00000077737; ENSMUSP00000076918; ENSMUSG00000026918 [Q8K2F0-1] ENSMUST00000113941; ENSMUSP00000109574; ENSMUSG00000026918 [Q8K2F0-2] ENSMUST00000164296; ENSMUSP00000128812; ENSMUSG00000026918 [Q8K2F0-1] |
GeneIDi | 67382 |
KEGGi | mmu:67382 |
UCSCi | uc008ixm.2, mouse [Q8K2F0-1] |
Organism-specific databases
CTDi | 8019 |
MGIi | MGI:1914632, Brd3 |
VEuPathDBi | HostDB:ENSMUSG00000026918 |
Phylogenomic databases
eggNOGi | KOG1474, Eukaryota |
GeneTreei | ENSGT00940000153385 |
InParanoidi | Q8K2F0 |
OMAi | CYAFNPD |
TreeFami | TF317345 |
Miscellaneous databases
BioGRID-ORCSi | 67382, 1 hit in 77 CRISPR screens |
ChiTaRSi | Brd3, mouse |
EvolutionaryTracei | Q8K2F0 |
PROi | PR:Q8K2F0 |
RNActi | Q8K2F0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026918, Expressed in undifferentiated genital tubercle and 306 other tissues |
ExpressionAtlasi | Q8K2F0, baseline and differential |
Genevisiblei | Q8K2F0, MM |
Family and domain databases
CDDi | cd05497, Bromo_Brdt_I_like, 1 hit cd05498, Bromo_Brdt_II_like, 1 hit |
Gene3Di | 1.20.1270.220, 1 hit 1.20.920.10, 2 hits |
IDEALi | IID50153 |
InterProi | View protein in InterPro IPR043508, Bromo_Brdt_I IPR043509, Bromo_Brdt_II IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR018359, Bromodomain_CS IPR027353, NET_dom IPR038336, NET_sf |
Pfami | View protein in Pfam PF17035, BET, 1 hit PF00439, Bromodomain, 2 hits |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 2 hits |
SUPFAMi | SSF47370, SSF47370, 2 hits |
PROSITEi | View protein in PROSITE PS00633, BROMODOMAIN_1, 2 hits PS50014, BROMODOMAIN_2, 2 hits PS51525, NET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BRD3_MOUSE | |
Accessioni | Q8K2F0Primary (citable) accession number: Q8K2F0 Secondary accession number(s): Q8C665, Q8CAX7, Q9JI25 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | May 25, 2022 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references