UniProtKB - Q8K2A8 (ALG3_MOUSE)
Protein
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Gene
Alg3
Organism
Mus musculus (Mouse)
Status
Functioni
Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.
Catalytic activityi
- α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-mannosyl phosphate = α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+EC:2.4.1.258
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- alpha-1,3-mannosyltransferase activity Source: MGI
- dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity Source: GO_Central
GO - Biological processi
- protein glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT58, Glycosyltransferase Family 58 |
Names & Taxonomyi
Protein namesi | Recommended name: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)Alternative name(s): Asparagine-linked glycosylation protein 3 homolog Dol-P-Man-dependent alpha(1-3)-mannosyltransferase Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase Not56-like protein |
Gene namesi | Name:Alg3 Synonyms:Not, Not56l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1098592, Alg3 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 41 – 61 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 95 – 115 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 123 – 143 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 149 – 169 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 203 – 223 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 231 – 251 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 289 – 309 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 332 – 352 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 356 – 376 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 407 – 427 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080567 | 1 – 438 | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferaseAdd BLAST | 438 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8K2A8 |
MaxQBi | Q8K2A8 |
PaxDbi | Q8K2A8 |
PeptideAtlasi | Q8K2A8 |
PRIDEi | Q8K2A8 |
PTM databases
PhosphoSitePlusi | Q8K2A8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000033809, Expressed in ear and 282 other tissues |
ExpressionAtlasi | Q8K2A8, baseline and differential |
Genevisiblei | Q8K2A8, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 228994, 3 interactors |
STRINGi | 10090.ENSMUSP00000045272 |
Miscellaneous databases
RNActi | Q8K2A8, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 58 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2762, Eukaryota |
GeneTreei | ENSGT00390000013904 |
HOGENOMi | CLU_035382_3_0_1 |
InParanoidi | Q8K2A8 |
OMAi | AAHVYIY |
OrthoDBi | 774318at2759 |
TreeFami | TF105870 |
Family and domain databases
InterProi | View protein in InterPro IPR007873, Glycosyltransferase_ALG3 |
PANTHERi | PTHR12646, PTHR12646, 1 hit |
Pfami | View protein in Pfam PF05208, ALG3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 12 potential isoforms that are computationally mapped.Show allAlign All
Q8K2A8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAGLRKRGQ PASVGQPAGI WKQWLQRAWQ ERYLLLREPR YTLLVASCLC
60 70 80 90 100
IAEVGITFWV IHRVAYTEID WKAYMAQVEG FINGTYDYTQ LQGDTGPLVY
110 120 130 140 150
PAGFLYIFTG LFYATDRGTD IPMAQNIFAV LYLVTLVLVF LIYHQTSKVP
160 170 180 190 200
PFVFFFMCCA SYRVHSIFVL RLFNDPVAMA LLFLSINLFL AQCWSWGCCC
210 220 230 240 250
FSLAVSVKMN VLLFAPGLLF LLLTQFGFRG ALPKLAICAA LQVVLGLPFL
260 270 280 290 300
LENPIGYLSR SFDLGRQFLF QWTVNWRFLP ETIFLHRAFH LALLAAHLSL
310 320 330 340 350
LLLFALCRWH RTGESILALL KDPSKRKVPP QALTPNQIVS ILFTSNFIGI
360 370 380 390 400
CFSRSLHYQF YVWYFHTLPY LLWAMPARWL THLLRLLVLG LIELSWNTYP
410 420 430
STSFSSAALH LCHAVVLLQL WLSPESFPKS IQPSRKTH
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6XUH0 | F6XUH0_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 173 | Annotation score: | ||
A0A338P6K1 | A0A338P6K1_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 134 | Annotation score: | ||
A0A338P6N0 | A0A338P6N0_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 208 | Annotation score: | ||
A0A338P722 | A0A338P722_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 188 | Annotation score: | ||
A0A338P726 | A0A338P726_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 316 | Annotation score: | ||
A0A338P6Q6 | A0A338P6Q6_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 266 | Annotation score: | ||
A0A338P6Y8 | A0A338P6Y8_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 364 | Annotation score: | ||
A0A338P794 | A0A338P794_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 242 | Annotation score: | ||
D6RCW8 | D6RCW8_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 107 | Annotation score: | ||
A0A338P7B5 | A0A338P7B5_MOUSE | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol al... | Alg3 | 69 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 354 | R → H in AAH31893 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC087898 Genomic DNA No translation available. BC031893 mRNA Translation: AAH31893.1 |
CCDSi | CCDS37289.1 |
RefSeqi | NP_666051.2, NM_145939.2 |
Genome annotation databases
Ensembli | ENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809 |
GeneIDi | 208624 |
KEGGi | mmu:208624 |
UCSCi | uc007yqe.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC087898 Genomic DNA No translation available. BC031893 mRNA Translation: AAH31893.1 |
CCDSi | CCDS37289.1 |
RefSeqi | NP_666051.2, NM_145939.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 228994, 3 interactors |
STRINGi | 10090.ENSMUSP00000045272 |
Protein family/group databases
CAZyi | GT58, Glycosyltransferase Family 58 |
PTM databases
PhosphoSitePlusi | Q8K2A8 |
Proteomic databases
EPDi | Q8K2A8 |
MaxQBi | Q8K2A8 |
PaxDbi | Q8K2A8 |
PeptideAtlasi | Q8K2A8 |
PRIDEi | Q8K2A8 |
Protocols and materials databases
Antibodypediai | 52417, 12 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809 |
GeneIDi | 208624 |
KEGGi | mmu:208624 |
UCSCi | uc007yqe.2, mouse |
Organism-specific databases
CTDi | 10195 |
MGIi | MGI:1098592, Alg3 |
Phylogenomic databases
eggNOGi | KOG2762, Eukaryota |
GeneTreei | ENSGT00390000013904 |
HOGENOMi | CLU_035382_3_0_1 |
InParanoidi | Q8K2A8 |
OMAi | AAHVYIY |
OrthoDBi | 774318at2759 |
TreeFami | TF105870 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Miscellaneous databases
BioGRID-ORCSi | 208624, 8 hits in 19 CRISPR screens |
ChiTaRSi | Psen2, mouse |
PROi | PR:Q8K2A8 |
RNActi | Q8K2A8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033809, Expressed in ear and 282 other tissues |
ExpressionAtlasi | Q8K2A8, baseline and differential |
Genevisiblei | Q8K2A8, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007873, Glycosyltransferase_ALG3 |
PANTHERi | PTHR12646, PTHR12646, 1 hit |
Pfami | View protein in Pfam PF05208, ALG3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALG3_MOUSE | |
Accessioni | Q8K2A8Primary (citable) accession number: Q8K2A8 Secondary accession number(s): E9QKP9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families