UniProtKB - Q8K1Z6 (GPR18_MOUSE)
Protein
N-arachidonyl glycine receptor
Gene
Gpr18
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (By similarity). However, conflicting results about the role of NAGly as an agonist are reported (PubMed:23104136). Can also be activated by plant-derived and synthetic cannabinoid agonists (By similarity). The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (By similarity). May contribute to regulation of the immune system (By similarity). Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs) in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (PubMed:25348153, PubMed:26197390). Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (PubMed:23461720, PubMed:27893106). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (By similarity).By similarity5 Publications
GO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
GO - Biological processi
- CD8-positive, alpha-beta intraepithelial T cell differentiation Source: MGI
- CD8-positive, gamma-delta intraepithelial T cell differentiation Source: MGI
- negative regulation of leukocyte chemotaxis Source: MGI
- negative regulation of tumor necrosis factor production Source: MGI
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway Source: GO_Central
- positive regulation of Rho protein signal transduction Source: GO_Central
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: N-arachidonyl glycine receptorShort name: NAGly receptor Alternative name(s): G-protein coupled receptor 18 |
Gene namesi | Name:Gpr18Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107859, Gpr18 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasmic vesicle membrane By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 26 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 27 – 47 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 48 – 56 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 57 – 77 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 78 – 95 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 96 – 116 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 117 – 138 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 139 – 159 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 160 – 191 | ExtracellularSequence analysisAdd BLAST | 32 | |
Transmembranei | 192 – 212 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 213 – 236 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 237 – 257 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 258 – 268 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 269 – 289 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 290 – 331 | CytoplasmicSequence analysisAdd BLAST | 42 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278174 | 1 – 331 | N-arachidonyl glycine receptorAdd BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 14 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 94 ↔ 172 | PROSITE-ProRule annotation | ||
Glycosylationi | 188 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 322 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q8K1Z6 |
PRIDEi | Q8K1Z6 |
PTM databases
GlyGeni | Q8K1Z6, 2 sites |
iPTMneti | Q8K1Z6 |
PhosphoSitePlusi | Q8K1Z6 |
Expressioni
Tissue specificityi
Expressed in the eye including cornea, retina, iris and ciliary epithelium (at protein level) (PubMed:23461720). Expressed in spleen, liver and lymphocytes with highest expression levels in intestinal intraepithelial lymphocytes (PubMed:25348153, PubMed:26197390).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000050350, Expressed in mesenteric lymph node and 76 other tissues |
Genevisiblei | Q8K1Z6, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q8K1Z6, 1 interactor |
STRINGi | 10090.ENSMUSP00000061410 |
Miscellaneous databases
RNActi | Q8K1Z6, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QT1V, Eukaryota |
GeneTreei | ENSGT01010000222366 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q8K1Z6 |
OMAi | CIMIGCY |
OrthoDBi | 1122184at2759 |
PhylomeDBi | Q8K1Z6 |
TreeFami | TF330775 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR028335, GPR18 |
PANTHERi | PTHR24232:SF1, PTHR24232:SF1, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8K1Z6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATLSNHNQL DLSNGSHPEE YKIAALVFYS CIFLIGLFVN VTALWVFSCT
60 70 80 90 100
TKKRTTVTIY MMNVALLDLV FILSLPFRMF YYAKGEWPFG EYFCHILGAL
110 120 130 140 150
VVFYPSLALW LLAFISADRY MAIVQPKYAK ELKNTGKAVL ACGGVWVMTL
160 170 180 190 200
TTTVPLLLLY EDPDKASSPA TCLKISDITH LKAVNVLNFT RLIFFFLIPL
210 220 230 240 250
FIMIGCYVVI IHSLLRGQTS KLKPKVKEKS IRIIMTLLLQ VLVCFVPFHI
260 270 280 290 300
CFAVLMLQGQ ENSYSPWGAF TTFLMNLSTC LDVVLYYIVS KQFQARVISV
310 320 330
MLYRNYLRSV RRKSVRSGSL RSLSNMNSEM L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 23 | I → V in BAE42987 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK156039 mRNA Translation: BAE33557.1 AK156868 mRNA Translation: BAE33880.1 AK157029 mRNA Translation: BAE33938.1 AK172401 mRNA Translation: BAE42987.1 BC034872 mRNA Translation: AAH34872.1 |
CCDSi | CCDS27342.1 |
RefSeqi | NP_877958.1, NM_182806.1 |
Genome annotation databases
Ensembli | ENSMUST00000055475; ENSMUSP00000061410; ENSMUSG00000050350 |
GeneIDi | 110168 |
KEGGi | mmu:110168 |
UCSCi | uc007vap.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK156039 mRNA Translation: BAE33557.1 AK156868 mRNA Translation: BAE33880.1 AK157029 mRNA Translation: BAE33938.1 AK172401 mRNA Translation: BAE42987.1 BC034872 mRNA Translation: AAH34872.1 |
CCDSi | CCDS27342.1 |
RefSeqi | NP_877958.1, NM_182806.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8K1Z6, 1 interactor |
STRINGi | 10090.ENSMUSP00000061410 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q8K1Z6, 2 sites |
iPTMneti | Q8K1Z6 |
PhosphoSitePlusi | Q8K1Z6 |
Proteomic databases
PaxDbi | Q8K1Z6 |
PRIDEi | Q8K1Z6 |
Protocols and materials databases
Antibodypediai | 2954, 358 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000055475; ENSMUSP00000061410; ENSMUSG00000050350 |
GeneIDi | 110168 |
KEGGi | mmu:110168 |
UCSCi | uc007vap.1, mouse |
Organism-specific databases
CTDi | 2841 |
MGIi | MGI:107859, Gpr18 |
Phylogenomic databases
eggNOGi | ENOG502QT1V, Eukaryota |
GeneTreei | ENSGT01010000222366 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q8K1Z6 |
OMAi | CIMIGCY |
OrthoDBi | 1122184at2759 |
PhylomeDBi | Q8K1Z6 |
TreeFami | TF330775 |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 110168, 0 hits in 17 CRISPR screens |
PROi | PR:Q8K1Z6 |
RNActi | Q8K1Z6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050350, Expressed in mesenteric lymph node and 76 other tissues |
Genevisiblei | Q8K1Z6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR028335, GPR18 |
PANTHERi | PTHR24232:SF1, PTHR24232:SF1, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPR18_MOUSE | |
Accessioni | Q8K1Z6Primary (citable) accession number: Q8K1Z6 Secondary accession number(s): Q3T9N3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries