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UniProtKB - Q8K1S7 (KREM2_MOUSE)
Protein
Kremen protein 2
Gene
Kremen2
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6 (PubMed:12050670).
Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation (PubMed:18505822).
2 PublicationsGO - Biological processi
- limb development Source: UniProtKB
- negative regulation of ossification Source: UniProtKB
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-MMU-3772470, Negative regulation of TCF-dependent signaling by WNT ligand antagonists |
Names & Taxonomyi
Protein namesi | Recommended name: Kremen protein 2Alternative name(s): Dickkopf receptor 2 Kringle domain-containing transmembrane protein 2 Kringle-containing protein marking the eye and the nose |
Gene namesi | Name:Kremen2 |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920266, Kremen2 |
VEuPathDBi | HostDB:ENSMUSG00000040680 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type I membrane protein Curated
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 363 | ExtracellularSequence analysisAdd BLAST | 339 | |
Transmembranei | 364 – 386 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 387 – 461 | CytoplasmicSequence analysisAdd BLAST | 75 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Animals with a double knockout of KREM1 and KREM2 exhibit enhanced Wnt signaling accompanied by ectopic postaxial forelimb digits and expanded apical ectodermal ridges. They also exhibit increased bone volume and bone formation rates. Triple knockout mice KREM1/KREM2/DKK1 exhibit enhanced growth of ectopic digits.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000021569 | 25 – 461 | Kremen protein 2Add BLAST | 437 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 35 ↔ 118 | By similarity | ||
Glycosylationi | 48 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 59 ↔ 99 | By similarity | ||
Disulfide bondi | 88 ↔ 113 | By similarity | ||
Disulfide bondi | 218 ↔ 244 | By similarity | ||
Glycosylationi | 221 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 243 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 350 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q8K1S7 |
PRIDEi | Q8K1S7 |
PTM databases
GlyGeni | Q8K1S7, 4 sites |
PhosphoSitePlusi | Q8K1S7 |
Expressioni
Developmental stagei
Expressed in the developing brain and developing limb buds.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040680, Expressed in embryo and 72 other tissues |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 215713, 1 interactor |
CORUMi | Q8K1S7 |
STRINGi | 10090.ENSMUSP00000046369 |
Miscellaneous databases
RNActi | Q8K1S7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 118 | KringlePROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 120 – 214 | WSCPROSITE-ProRule annotationCuratedAdd BLAST | 95 | |
Domaini | 218 – 325 | CUBPROSITE-ProRule annotationCuratedAdd BLAST | 108 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 329 – 357 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 429 – 452 | DisorderedSequence analysisAdd BLAST | 24 |
Domaini
Binding to ERLEC1 is mediated by the oligosaccharides linked to the kringle domain.1 Publication
Keywords - Domaini
Kringle, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4157, Eukaryota |
GeneTreei | ENSGT00940000162126 |
HOGENOMi | CLU_047976_0_0_1 |
InParanoidi | Q8K1S7 |
OMAi | RNCSWVV |
OrthoDBi | 516719at2759 |
PhylomeDBi | Q8K1S7 |
TreeFami | TF331319 |
Family and domain databases
CDDi | cd00041, CUB, 1 hit cd00108, KR, 1 hit |
Gene3Di | 2.40.20.10, 1 hit 2.60.120.290, 1 hit |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR017076, Kremen IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR035914, Sperma_CUB_dom_sf IPR002889, WSC_carb-bd |
Pfami | View protein in Pfam PF00431, CUB, 1 hit PF00051, Kringle, 1 hit PF01822, WSC, 1 hit |
PIRSFi | PIRSF036961, Kremen, 1 hit |
SMARTi | View protein in SMART SM00042, CUB, 1 hit SM00130, KR, 1 hit SM00321, WSC, 1 hit |
SUPFAMi | SSF49854, SSF49854, 1 hit SSF57440, SSF57440, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS51212, WSC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8K1S7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTPHLQGFL LLFPLLLRLH GASAGSLHSP GLSECFQVNG ADYRGHQNYT
60 70 80 90 100
GPRGAGRPCL FWDQTQQHSY SSASDPQGRW GLGAHNFCRN PDGDVQPWCY
110 120 130 140 150
VAETEEGIYW RYCDIPTCHM PGYLGCFVDS GAPPALSGPS GTSTKLTVQV
160 170 180 190 200
CLRFCRMKGY QLAGVEAGYA CFCGSESDLA RGRPAPATDC DQICFGHPGQ
210 220 230 240 250
LCGGDGRLGI YEVSVGSCQG NWSAPQGVIY SPDFPDEYGP DRNCSWVLGQ
260 270 280 290 300
LGAVLELTFR LFELADSRDR LELRDVSSGN LLRAFDGAHP PPPGPLRLRT
310 320 330 340 350
AALLLTFRSD ARGHAQGFAL TYRGLQDTVE GRASPEDSTE SLAGDPDGAN
360 370 380 390 400
ASCSPKPGAA QASIGARVFS TVTAFSVLLL LLLSLLRLLR RRSCLLAPGK
410 420 430 440 450
GSLAMGPSRG PGRSWAVWYR RPRGVALPCP PGDSQAEGPA AGYRPLSASS
460
QSSLRSLVSA L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ457192 mRNA Translation: CAD29805.1 BC120532 mRNA Translation: AAI20533.1 BC125438 mRNA Translation: AAI25439.1 |
CCDSi | CCDS28462.1 |
RefSeqi | NP_082692.1, NM_028416.2 XP_006525047.1, XM_006524984.3 |
Genome annotation databases
Ensembli | ENSMUST00000046525; ENSMUSP00000046369; ENSMUSG00000040680 |
GeneIDi | 73016 |
KEGGi | mmu:73016 |
UCSCi | uc008atd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ457192 mRNA Translation: CAD29805.1 BC120532 mRNA Translation: AAI20533.1 BC125438 mRNA Translation: AAI25439.1 |
CCDSi | CCDS28462.1 |
RefSeqi | NP_082692.1, NM_028416.2 XP_006525047.1, XM_006524984.3 |
3D structure databases
SMRi | Q8K1S7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 215713, 1 interactor |
CORUMi | Q8K1S7 |
STRINGi | 10090.ENSMUSP00000046369 |
PTM databases
GlyGeni | Q8K1S7, 4 sites |
PhosphoSitePlusi | Q8K1S7 |
Proteomic databases
PaxDbi | Q8K1S7 |
PRIDEi | Q8K1S7 |
Protocols and materials databases
Antibodypediai | 1159, 122 antibodies from 25 providers |
DNASUi | 73016 |
Genome annotation databases
Ensembli | ENSMUST00000046525; ENSMUSP00000046369; ENSMUSG00000040680 |
GeneIDi | 73016 |
KEGGi | mmu:73016 |
UCSCi | uc008atd.1, mouse |
Organism-specific databases
CTDi | 79412 |
MGIi | MGI:1920266, Kremen2 |
VEuPathDBi | HostDB:ENSMUSG00000040680 |
Phylogenomic databases
eggNOGi | KOG4157, Eukaryota |
GeneTreei | ENSGT00940000162126 |
HOGENOMi | CLU_047976_0_0_1 |
InParanoidi | Q8K1S7 |
OMAi | RNCSWVV |
OrthoDBi | 516719at2759 |
PhylomeDBi | Q8K1S7 |
TreeFami | TF331319 |
Enzyme and pathway databases
Reactomei | R-MMU-3772470, Negative regulation of TCF-dependent signaling by WNT ligand antagonists |
Miscellaneous databases
BioGRID-ORCSi | 73016, 4 hits in 65 CRISPR screens |
PROi | PR:Q8K1S7 |
RNActi | Q8K1S7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040680, Expressed in embryo and 72 other tissues |
Family and domain databases
CDDi | cd00041, CUB, 1 hit cd00108, KR, 1 hit |
Gene3Di | 2.40.20.10, 1 hit 2.60.120.290, 1 hit |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR017076, Kremen IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR035914, Sperma_CUB_dom_sf IPR002889, WSC_carb-bd |
Pfami | View protein in Pfam PF00431, CUB, 1 hit PF00051, Kringle, 1 hit PF01822, WSC, 1 hit |
PIRSFi | PIRSF036961, Kremen, 1 hit |
SMARTi | View protein in SMART SM00042, CUB, 1 hit SM00130, KR, 1 hit SM00321, WSC, 1 hit |
SUPFAMi | SSF49854, SSF49854, 1 hit SSF57440, SSF57440, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS51212, WSC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KREM2_MOUSE | |
Accessioni | Q8K1S7Primary (citable) accession number: Q8K1S7 Secondary accession number(s): Q0VBR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 23, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot