UniProtKB - Q8K1R7 (NEK9_MOUSE)
Serine/threonine-protein kinase Nek9
Nek9
Functioni
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (By similarity).
By similarityCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 81 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 176 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 58 – 66 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein kinase binding Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase Nek9 (EC:2.7.11.1)Alternative name(s): Nercc1 kinase Never in mitosis A-related kinase 9 Short name: NimA-related protein kinase 9 |
Gene namesi | Name:Nek9 Synonyms:Nercc |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2387995, Nek9 |
VEuPathDBi | HostDB:ENSMUSG00000034290 |
Subcellular locationi
Cytoskeleton
- centrosome Source: Ensembl
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000086436 | 2 – 984 | Serine/threonine-protein kinase Nek9Add BLAST | 983 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 16 | PhosphoserineBy similarity | 1 | |
Modified residuei | 20 | PhosphoserineBy similarity | 1 | |
Modified residuei | 52 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 76 | PhosphoserineBy similarity | 1 | |
Modified residuei | 210 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 254 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 331 | PhosphoserineCombined sources | 1 | |
Modified residuei | 333 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 741 | PhosphoserineBy similarity | 1 | |
Modified residuei | 808 | PhosphoserineBy similarity | 1 | |
Modified residuei | 839 | PhosphoserineBy similarity | 1 | |
Modified residuei | 891 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 949 | PhosphoserineBy similarity | 1 | |
Modified residuei | 983 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8K1R7 |
jPOSTi | Q8K1R7 |
MaxQBi | Q8K1R7 |
PaxDbi | Q8K1R7 |
PRIDEi | Q8K1R7 |
ProteomicsDBi | 287368 |
PTM databases
iPTMneti | Q8K1R7 |
PhosphoSitePlusi | Q8K1R7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034290, Expressed in ureter and 304 other tissues |
Genevisiblei | Q8K1R7, MM |
Interactioni
Subunit structurei
Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP.
Interacts with NEK6, NEK7 and BICD2.
Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex.
Interacts with DYNLL1; phosphorylation at Ser-949 strongly reduces DYNLL1 binding (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 229954, 30 interactors |
DIPi | DIP-56880N |
IntActi | Q8K1R7, 28 interactors |
MINTi | Q8K1R7 |
STRINGi | 10090.ENSMUSP00000049056 |
Miscellaneous databases
RNActi | Q8K1R7, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8K1R7 |
SMRi | Q8K1R7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 52 – 308 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 257 | |
Repeati | 388 – 444 | RCC1 1Add BLAST | 57 | |
Repeati | 445 – 498 | RCC1 2Add BLAST | 54 | |
Repeati | 499 – 550 | RCC1 3Add BLAST | 52 | |
Repeati | 551 – 615 | RCC1 4Add BLAST | 65 | |
Repeati | 616 – 668 | RCC1 5Add BLAST | 53 | |
Repeati | 669 – 726 | RCC1 6Add BLAST | 58 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 43 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 732 – 896 | Interaction with NEK6By similarityAdd BLAST | 165 | |
Regioni | 744 – 790 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 940 – 984 | DisorderedSequence analysisAdd BLAST | 45 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 896 – 945 | Sequence analysisAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 953 – 967 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0589, Eukaryota KOG1426, Eukaryota |
GeneTreei | ENSGT00940000156145 |
HOGENOMi | CLU_000288_123_1_1 |
InParanoidi | Q8K1R7 |
OMAi | TKRMSCD |
OrthoDBi | 1062377at2759 |
PhylomeDBi | Q8K1R7 |
TreeFami | TF106472 |
Family and domain databases
CDDi | cd08221, STKc_Nek9, 1 hit |
Gene3Di | 2.130.10.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR042767, Nek9_STKc IPR000719, Prot_kinase_dom IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00415, RCC1, 4 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50012, RCC1_3, 6 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSVLGEYERH CDSINSDFGS ESGGGGDSGP GPSAVPGPRA GGGAAEQEEL
60 70 80 90 100
HYIPIRVLGR GAFGEATLYR RTEDDSLVVW KEVDLTRLSE KERRDALNEI
110 120 130 140 150
VILALLQHDN IIAYYNHFMD NTTLLIELEY CNGGNLYDKI LRQKDKLFEE
160 170 180 190 200
EMVVWYLFQI VSAVSCIHKA GILHRDIKTL NIFLTKANLI KLGDYGLAKK
210 220 230 240 250
LNSEYSMAET LVGTPYYMSP ELCQGVKYNF KSDIWAVGCV IFELLTLKRT
260 270 280 290 300
FDATNPLNLC VKIVQGIRAM EVDSSQYSLE LIQLVHACLD QDPEQRPAAD
310 320 330 340 350
ALLDLPLLRT RRREMEEKVT LLNAPTKRPR SSTVTEAPIA VVTSRTSEVY
360 370 380 390 400
VWGGGKSTPQ KLDVIKSGCS ARQVCAGNTH FAVVTVEKEL YTWVNMQGGT
410 420 430 440 450
KLHGQLGHGD KASYRQPKHV EKLQGKAIHQ VSCGDDFTVC VTDEGQLYAF
460 470 480 490 500
GSDYYGCMGV DKVSGPEVLE PMQLNFFLSN PVEQVSCGDN HVVVLTRNKE
510 520 530 540 550
VYSWGCGEYG RLGLDSEEDY YTPQRVDVPK ALIIVAVQCG CDGTFLLTQS
560 570 580 590 600
GKVLACGLNE FNKLGLNQCM SGIINHEAYH EVPYTTSFTL AKQLSFYKIR
610 620 630 640 650
TIAPGKTHTA AIDERGRLLT FGCNKCGQLG VGNYKKRLGI NLLGGPLGGK
660 670 680 690 700
QVIRVSCGDE FTIAATDDNH IFAWGNGGNG RLAMTPTERP HGSDICTSWP
710 720 730 740 750
RPIFGSLHHV PDLSCRGWHT ILIVEKVLNS KTIRSNSSGL SIGTVVQSSS
760 770 780 790 800
PGGGIGGGGG GGGGGGGEEE DSQQESETPD PSGGFRGTME ADRGMEGLIS
810 820 830 840 850
PTEAVGNSCG ASSSCPGWLR KELENAEFIP MPDSPAPLSA AFSQSEKDTL
860 870 880 890 900
PYEELQGLKV ASEVPPEPQR AAGAWPPRLD PAVPCVGKAL TSAACACSAL
910 920 930 940 950
QVEVDRLQAL VLKCLEEQQK LQQENLQMFT QLQKLNKKLE GGQQVGMHSR
960 970 980
GTQTAKEEME MDPKPDLDSE SWCLLGTDSC RPSL
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 929 | F → S in CAD34025 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ489828 mRNA Translation: CAD34025.1 CH466590 Genomic DNA Translation: EDL02865.1 BC024926 mRNA Translation: AAH24926.1 Different initiation. BC117971 mRNA Translation: AAI17972.1 BC117972 mRNA Translation: AAI17973.1 |
CCDSi | CCDS26057.1 |
RefSeqi | NP_660120.2, NM_145138.2 |
Genome annotation databases
Ensembli | ENSMUST00000040992; ENSMUSP00000049056; ENSMUSG00000034290 |
GeneIDi | 217718 |
KEGGi | mmu:217718 |
UCSCi | uc007ogx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ489828 mRNA Translation: CAD34025.1 CH466590 Genomic DNA Translation: EDL02865.1 BC024926 mRNA Translation: AAH24926.1 Different initiation. BC117971 mRNA Translation: AAI17972.1 BC117972 mRNA Translation: AAI17973.1 |
CCDSi | CCDS26057.1 |
RefSeqi | NP_660120.2, NM_145138.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5DE2 | X-ray | 2.78 | C/D | 817-835 | [»] | |
AlphaFoldDBi | Q8K1R7 | |||||
SMRi | Q8K1R7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 229954, 30 interactors |
DIPi | DIP-56880N |
IntActi | Q8K1R7, 28 interactors |
MINTi | Q8K1R7 |
STRINGi | 10090.ENSMUSP00000049056 |
PTM databases
iPTMneti | Q8K1R7 |
PhosphoSitePlusi | Q8K1R7 |
Proteomic databases
EPDi | Q8K1R7 |
jPOSTi | Q8K1R7 |
MaxQBi | Q8K1R7 |
PaxDbi | Q8K1R7 |
PRIDEi | Q8K1R7 |
ProteomicsDBi | 287368 |
Protocols and materials databases
Antibodypediai | 104, 491 antibodies from 33 providers |
DNASUi | 217718 |
Genome annotation databases
Ensembli | ENSMUST00000040992; ENSMUSP00000049056; ENSMUSG00000034290 |
GeneIDi | 217718 |
KEGGi | mmu:217718 |
UCSCi | uc007ogx.2, mouse |
Organism-specific databases
CTDi | 91754 |
MGIi | MGI:2387995, Nek9 |
VEuPathDBi | HostDB:ENSMUSG00000034290 |
Phylogenomic databases
eggNOGi | KOG0589, Eukaryota KOG1426, Eukaryota |
GeneTreei | ENSGT00940000156145 |
HOGENOMi | CLU_000288_123_1_1 |
InParanoidi | Q8K1R7 |
OMAi | TKRMSCD |
OrthoDBi | 1062377at2759 |
PhylomeDBi | Q8K1R7 |
TreeFami | TF106472 |
Enzyme and pathway databases
Reactomei | R-MMU-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 |
Miscellaneous databases
BioGRID-ORCSi | 217718, 4 hits in 77 CRISPR screens |
ChiTaRSi | Nek9, mouse |
PROi | PR:Q8K1R7 |
RNActi | Q8K1R7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034290, Expressed in ureter and 304 other tissues |
Genevisiblei | Q8K1R7, MM |
Family and domain databases
CDDi | cd08221, STKc_Nek9, 1 hit |
Gene3Di | 2.130.10.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR042767, Nek9_STKc IPR000719, Prot_kinase_dom IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00415, RCC1, 4 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50012, RCC1_3, 6 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NEK9_MOUSE | |
Accessioni | Q8K1R7Primary (citable) accession number: Q8K1R7 Secondary accession number(s): Q148U2, Q8R3P1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families