UniProtKB - Q8K1M4 (GLIS1_MOUSE)
Zinc finger protein GLIS1
Glis1
Functioni
Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807).
Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312).
By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251).
Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 366 – 391 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 400 – 427 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 433 – 457 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 463 – 487 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 493 – 517 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- fat cell differentiation Source: UniProtKB
- negative regulation of cell fate commitment Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- osteoblast differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein GLIS1Alternative name(s): GLI-similar 1 Gli homologous protein 11 Publication Short name: GliH11 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2386723, Glis1 |
VEuPathDBi | HostDB:ENSMUSG00000034762 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Biotechnological usei
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047210 | 1 – 789 | Zinc finger protein GLIS1Add BLAST | 789 |
Proteomic databases
MaxQBi | Q8K1M4 |
PaxDbi | Q8K1M4 |
PRIDEi | Q8K1M4 |
ProteomicsDBi | 267451 [Q8K1M4-1] 267452 [Q8K1M4-2] |
PTM databases
iPTMneti | Q8K1M4 |
PhosphoSitePlusi | Q8K1M4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000034762, Expressed in secondary oocyte and 174 other tissues |
ExpressionAtlasi | Q8K1M4, baseline and differential |
Genevisiblei | Q8K1M4, MM |
Interactioni
Subunit structurei
Interacts with KLF4.
Interacts with POU5F1 and/or POU5F1B.
Interacts with SOX2.
By similarityProtein-protein interaction databases
BioGRIDi | 230975, 16 interactors |
STRINGi | 10090.ENSMUSP00000035650 |
Miscellaneous databases
RNActi | Q8K1M4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 29 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 53 – 93 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 278 – 304 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 506 – 529 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 573 – 684 | DisorderedSequence analysisAdd BLAST | 112 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 511 – 527 | Bipartite nuclear localization signalSequence analysisAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 651 – 667 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 366 – 391 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 400 – 427 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 433 – 457 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 463 – 487 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 493 – 517 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000159218 |
InParanoidi | Q8K1M4 |
OMAi | GSSTNHN |
OrthoDBi | 703190at2759 |
PhylomeDBi | Q8K1M4 |
TreeFami | TF350216 |
Family and domain databases
InterProi | View protein in InterPro IPR043359, GLI-like IPR030430, GLIS1 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR45718, PTHR45718, 1 hit PTHR45718:SF3, PTHR45718:SF3, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHCEVAEALS DKRPKEAPGA PGQGRGPVSL GAHMAFRIAV SGGGCGDGNP
60 70 80 90 100
LDLLPRLPVP PPRAHDLLRP RSPRDYGVSK TGSGKVNGSY GHSSEKSLLD
110 120 130 140 150
LDLAEGPSPS CHQGLFLPAG TPPPRGHPPV CEKLLHFPHP NRSPRPQATF
160 170 180 190 200
VNGSLPAAQH IKQEALPDYQ AMVSAHTPLP THCRAPSSMG LPSDLDFPDR
210 220 230 240 250
GLTNPAPSCY LLGNEPISDL GPQPEAHLPE GSLKRCCLLG LPPTSSASSS
260 270 280 290 300
PCASSDINPV IHSSQTALVS CVNGLRSPPL PGDLGGPPKR SRPGPASSDG
310 320 330 340 350
QEGSLQLEAC RKSGFLKQEP MDEFSELFAP HHQGLPPPYP LPQLPTGPGL
360 370 380 390 400
GGLGLGLAGR MVAGRQACRW VDCCAAYEQQ EELVRHIEKS HIDQRKGEDF
410 420 430 440 450
TCFWAGCVRR YKPFNARYKL LIHMRVHSGE KPNKCMFEGC SKAFSRLENL
460 470 480 490 500
KIHLRSHTGE KPYLCQHPGC QKAFSNSSDR AKHQRTHLDT KPYACQIPGC
510 520 530 540 550
SKRYTDPSSL RKHVKAHSAK EQQVRKKLHT GADPEADVLS ECLSLQQLQA
560 570 580 590 600
STLLPASRGK GSQTLSQELL PGVYPGSVTP QNGLASGILS PSHDVPSRHH
610 620 630 640 650
PLEVPTGSHH HLSPLPTAES TRDGLGPSLL SPMVSPLKGL GPPPLPPASQ
660 670 680 690 700
SQSPGGQSFS TVPSKPTYPS FQSPPPLPSP QGYQGSFHSI QNCFPYADCY
710 720 730 740 750
RATEPAASRD GLVGDAHGFN PLRPSTYSSL STPLSAPGYE TLAETPCPPA
760 770 780
LQPQPAEDLV PSGPEDCGFF PNGAFDHCLS HIPSIYTDT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1ASP5 | B1ASP5_MOUSE | Zinc finger protein GLIS1 | Glis1 | 601 | Annotation score: | ||
A2A8S0 | A2A8S0_MOUSE | Zinc finger protein GLIS1 | Glis1 | 104 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 173 | V → A in AAM93156 (PubMed:12042312).Curated | 1 | |
Sequence conflicti | 354 | G → R in AAM93156 (PubMed:12042312).Curated | 1 | |
Sequence conflicti | 378 | E → G in AAH66157 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 384 | V → E in AAH66157 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 466 | Q → R in AAM93156 (PubMed:12042312).Curated | 1 | |
Sequence conflicti | 634 | V → A in AAH66157 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 668 | Y → S in AAH66157 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_051623 | 1 – 171 | Missing in isoform 2. 1 PublicationAdd BLAST | 171 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF486579 mRNA Translation: AAM93156.1 AF220434 mRNA Translation: AAM12150.1 BC066157 mRNA Translation: AAH66157.1 Different initiation. |
CCDSi | CCDS38831.1 [Q8K1M4-1] |
RefSeqi | NP_671754.2, NM_147221.2 [Q8K1M4-1] XP_006503036.1, XM_006502973.3 [Q8K1M4-1] XP_006503037.1, XM_006502974.3 [Q8K1M4-1] XP_006503038.1, XM_006502975.1 [Q8K1M4-1] |
Genome annotation databases
Ensembli | ENSMUST00000046005; ENSMUSP00000035650; ENSMUSG00000034762 [Q8K1M4-1] |
GeneIDi | 230587 |
KEGGi | mmu:230587 |
UCSCi | uc008tzv.1, mouse [Q8K1M4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF486579 mRNA Translation: AAM93156.1 AF220434 mRNA Translation: AAM12150.1 BC066157 mRNA Translation: AAH66157.1 Different initiation. |
CCDSi | CCDS38831.1 [Q8K1M4-1] |
RefSeqi | NP_671754.2, NM_147221.2 [Q8K1M4-1] XP_006503036.1, XM_006502973.3 [Q8K1M4-1] XP_006503037.1, XM_006502974.3 [Q8K1M4-1] XP_006503038.1, XM_006502975.1 [Q8K1M4-1] |
3D structure databases
SMRi | Q8K1M4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230975, 16 interactors |
STRINGi | 10090.ENSMUSP00000035650 |
PTM databases
iPTMneti | Q8K1M4 |
PhosphoSitePlusi | Q8K1M4 |
Proteomic databases
MaxQBi | Q8K1M4 |
PaxDbi | Q8K1M4 |
PRIDEi | Q8K1M4 |
ProteomicsDBi | 267451 [Q8K1M4-1] 267452 [Q8K1M4-2] |
Protocols and materials databases
Antibodypediai | 748, 121 antibodies from 27 providers |
DNASUi | 230587 |
Genome annotation databases
Ensembli | ENSMUST00000046005; ENSMUSP00000035650; ENSMUSG00000034762 [Q8K1M4-1] |
GeneIDi | 230587 |
KEGGi | mmu:230587 |
UCSCi | uc008tzv.1, mouse [Q8K1M4-1] |
Organism-specific databases
CTDi | 148979 |
MGIi | MGI:2386723, Glis1 |
VEuPathDBi | HostDB:ENSMUSG00000034762 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000159218 |
InParanoidi | Q8K1M4 |
OMAi | GSSTNHN |
OrthoDBi | 703190at2759 |
PhylomeDBi | Q8K1M4 |
TreeFami | TF350216 |
Miscellaneous databases
BioGRID-ORCSi | 230587, 3 hits in 65 CRISPR screens |
ChiTaRSi | Glis1, mouse |
PROi | PR:Q8K1M4 |
RNActi | Q8K1M4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034762, Expressed in secondary oocyte and 174 other tissues |
ExpressionAtlasi | Q8K1M4, baseline and differential |
Genevisiblei | Q8K1M4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR043359, GLI-like IPR030430, GLIS1 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR45718, PTHR45718, 1 hit PTHR45718:SF3, PTHR45718:SF3, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GLIS1_MOUSE | |
Accessioni | Q8K1M4Primary (citable) accession number: Q8K1M4 Secondary accession number(s): Q6NZF6, Q8R4Z3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families