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Protein

Lactase-like protein

Gene

Lctl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Caution

Although it belongs to the glycosyl hydrolase 1 family, Asp-200 is present instead of the conserved Glu which is an active site residue. It is therefore expected that this protein lacks glycosidase activity.Curated

GO - Molecular functioni

  • beta-glucosidase activity Source: GO_Central

GO - Biological processi

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Lactase-like protein
Alternative name(s):
Klotho/lactase-phlorizin hydrolase-related protein
Gene namesi
Name:Lctl
Synonyms:Klph
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2183549 Lctl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 540ExtracellularSequence analysisAdd BLAST520
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 566CytoplasmicSequence analysis5

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000004225221 – 566Lactase-like proteinAdd BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi244N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8K1F9
PRIDEiQ8K1F9

PTM databases

PhosphoSitePlusiQ8K1F9

Expressioni

Tissue specificityi

Kidney and skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000032401 Expressed in 19 organ(s), highest expression level in brown adipose tissue
CleanExiMM_LCTL
ExpressionAtlasiQ8K1F9 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034969

Structurei

3D structure databases

ProteinModelPortaliQ8K1F9
SMRiQ8K1F9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
GeneTreeiENSGT00550000074452
HOGENOMiHOG000088630
HOVERGENiHBG053101
InParanoidiQ8K1F9
OMAiITERNYP
OrthoDBiEOG091G0035
PhylomeDBiQ8K1F9
TreeFamiTF314803

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8K1F9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKPVWVIILG WILLVPRVGT AWRGPPEEAS FYYGTFPPGF SWGVGSSAYQ
60 70 80 90 100
TEGAWDEDGK GPSIWDAFTH GRKEQVLGGD TADTACDSYY KVQEDIALLK
110 120 130 140 150
ELQVSHYRFS LSWPRLLPTG VRAEQVNKRG IKFYSDFIDA LLKSNITPVV
160 170 180 190 200
TLHHWDLPQM LQVAYGGWQN VSMTRYFSDY ADLCFEVFGD RVKHWLTFSD
210 220 230 240 250
PRTMVEKGYE TGLHAPGLRL QGTGLYVAAH HIIKAHAQAW HSYNNTWRSK
260 270 280 290 300
QHGLVGISLN CDWGEPVDID NPDDIEAAER YLQFCLGWFA NPIYAGDYPQ
310 320 330 340 350
VMKDHIGTKS AEQGLEMSRL PTFSLQEKSY LKGTSDFLGL GHFTTRYITQ
360 370 380 390 400
RKYPSHQGPS YQNDRDLVEL VDPNWPEMGS PWLYSVPWGF RRLLNFAQTQ
410 420 430 440 450
YGDPPIYVTE SGAPQKLHCT QFCDEWRIQY LKGYINEMLK AIKDGVDIKG
460 470 480 490 500
YTSWSLLDKF EWEKGYADKY GFYYVEFNVR NKPRYPKASV QYYKEIITAS
510 520 530 540 550
GFPNPQEVES WRLKALETCS INNQMLATEP LLRHMHVASE IVVPTVCALS
560
ILTAALMLTL LLRRRG
Length:566
Mass (Da):64,826
Last modified:October 1, 2002 - v1
Checksum:iEC50A5730275DD84
GO
Isoform 3 (identifier: Q8K1F9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     529-566: EPLLRHMHVA...MLTLLLRRRG → GDVAETGSPL...SSRDVYFSGS

Note: No experimental confirmation available.
Show »
Length:407
Mass (Da):46,955
Checksum:iDD10330AC0BA30E7
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RI22D6RI22_MOUSE
Lactase-like protein
Lctl
235Annotation score:
D3YTQ7D3YTQ7_MOUSE
Lactase-like protein
Lctl mCG_12791
409Annotation score:

Sequence cautioni

The sequence AAH30631 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0158331 – 159Missing in isoform 3. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_015834529 – 566EPLLR…LRRRG → GDVAETGSPLHLHLSKHLFR ITLLFCCRSSRDVYFSGS in isoform 3. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309072 mRNA Translation: AAM77699.1
BC030631 mRNA Translation: AAH30631.1 Different initiation.
CCDSiCCDS23274.1 [Q8K1F9-1]
RefSeqiNP_665834.1, NM_145835.2 [Q8K1F9-1]
UniGeneiMm.436581

Genome annotation databases

EnsembliENSMUST00000034969; ENSMUSP00000034969; ENSMUSG00000032401 [Q8K1F9-1]
GeneIDi235435
KEGGimmu:235435
UCSCiuc009qbl.1 mouse [Q8K1F9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309072 mRNA Translation: AAM77699.1
BC030631 mRNA Translation: AAH30631.1 Different initiation.
CCDSiCCDS23274.1 [Q8K1F9-1]
RefSeqiNP_665834.1, NM_145835.2 [Q8K1F9-1]
UniGeneiMm.436581

3D structure databases

ProteinModelPortaliQ8K1F9
SMRiQ8K1F9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034969

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

PTM databases

PhosphoSitePlusiQ8K1F9

Proteomic databases

PaxDbiQ8K1F9
PRIDEiQ8K1F9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034969; ENSMUSP00000034969; ENSMUSG00000032401 [Q8K1F9-1]
GeneIDi235435
KEGGimmu:235435
UCSCiuc009qbl.1 mouse [Q8K1F9-1]

Organism-specific databases

CTDi197021
MGIiMGI:2183549 Lctl

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
GeneTreeiENSGT00550000074452
HOGENOMiHOG000088630
HOVERGENiHBG053101
InParanoidiQ8K1F9
OMAiITERNYP
OrthoDBiEOG091G0035
PhylomeDBiQ8K1F9
TreeFamiTF314803

Miscellaneous databases

PROiPR:Q8K1F9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032401 Expressed in 19 organ(s), highest expression level in brown adipose tissue
CleanExiMM_LCTL
ExpressionAtlasiQ8K1F9 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLCTL_MOUSE
AccessioniPrimary (citable) accession number: Q8K1F9
Secondary accession number(s): Q8BPR1, Q8K2M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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