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Protein

Syntaxin-5

Gene

Stx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates endoplasmic reticulum to Golgi transport. Together with p115/USO1 and GM130/GOLGA2, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus.By similarity

Caution

It is uncertain whether Met-1 or Met-55 is the initiator. However, for the rat ortholog it has been shown that the equivalent alternative isoform is produced by alternative initiation at a conserved initiator methionine at position 55.Curated

GO - Molecular functioni

  • protein N-terminus binding Source: MGI
  • SNAP receptor activity Source: MGI
  • SNARE binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811438 Intra-Golgi traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-5
Gene namesi
Name:Stx5
Synonyms:Stx5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1928483 Stx5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 333CytoplasmicSequence analysisAdd BLAST333
Transmembranei334 – 354Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini355VesicularSequence analysis1

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102061 – 355Syntaxin-5Add BLAST355

Proteomic databases

EPDiQ8K1E0
PaxDbiQ8K1E0
PeptideAtlasiQ8K1E0
PRIDEiQ8K1E0

PTM databases

iPTMnetiQ8K1E0
PhosphoSitePlusiQ8K1E0
SwissPalmiQ8K1E0

Expressioni

Gene expression databases

BgeeiENSMUSG00000010110 Expressed in 291 organ(s), highest expression level in secondary oocyte
CleanExiMM_STX5A
ExpressionAtlasiQ8K1E0 baseline and differential
GenevisibleiQ8K1E0 MM

Interactioni

Subunit structurei

Part of a ternary complex containing STX5A, NSFL1C and VCP. Part of a unique SNARE complex composed of the Golgi SNAREs GOSR1, GOSR2, YKT6 and VTI1A (PubMed:9705316). Interacts with COG4 (By similarity). Interacts with GM130/GOLGA2 (By similarity). Interacts (via IxM motif) with SEC24C and SEC24D; mediates STX5 packaging into COPII-coated vesicles (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207947, 1 interactor
IntActiQ8K1E0, 1 interactor
MINTiQ8K1E0
STRINGi10090.ENSMUSP00000073136

Structurei

3D structure databases

ProteinModelPortaliQ8K1E0
SMRiQ8K1E0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini263 – 325t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili287 – 318Sequence analysisAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi245 – 247IxM motif; signal for cargo packaging into COPII-coated vesiclesBy similarity3

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0812 Eukaryota
ENOG410Y2MB LUCA
GeneTreeiENSGT00390000000652
HOGENOMiHOG000194860
HOVERGENiHBG001973
InParanoidiQ8K1E0
KOiK08490
OMAiQDRTNEF
PhylomeDBiQ8K1E0
TreeFamiTF315068

Family and domain databases

InterProiView protein in InterPro
IPR010989 SNARE
IPR021538 Syntaxin-5_N
IPR006012 Syntaxin/epimorphin_CS
IPR000727 T_SNARE_dom
PfamiView protein in Pfam
PF05739 SNARE, 1 hit
PF11416 Syntaxin-5_N, 1 hit
SMARTiView protein in SMART
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
PROSITEiView protein in PROSITE
PS00914 SYNTAXIN, 1 hit
PS50192 T_SNARE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8K1E0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AISSSSDSTP LPTPVALVPS
60 70 80 90 100
PPDTMSCRDR TQEFQSACKS LQSRQNGIQT SKPALHAARQ CSEFTLMARR
110 120 130 140 150
IGKDLSNTFA KLEKLTILAK RKSLFDDKAV EIEELTYIIK QDINSLNKQI
160 170 180 190 200
AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ SKLASMSNDF KSVLEVRTEN
210 220 230 240 250
LKQQRNRREQ FSRAPVSALP LAPNNLGGGP IILGAESRAS RDVAIDMMDP
260 270 280 290 300
RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET
310 320 330 340 350
IQRIDENVLG AQLDVEAAHS EILKYFQSVT SNRWLMVKIF LILIVFFIIF

VVFLA
Note: No experimental confirmation available.
Length:355
Mass (Da):39,713
Last modified:September 5, 2006 - v3
Checksum:i29CFD4EA5665CFEF
GO
Isoform 2 (identifier: Q8K1E0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Note: Produced by alternative initiation at Met-55 of isoform 1.
Show »
Length:301
Mass (Da):34,103
Checksum:i561706F552EB9D93
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BJ02H3BJ02_MOUSE
Syntaxin 5A
Stx5a
259Annotation score:
H3BKP3H3BKP3_MOUSE
Syntaxin 5A
Stx5a
109Annotation score:
I1E4X1I1E4X1_MOUSE
Syntaxin 5A
Stx5a
442Annotation score:
H3BJ89H3BJ89_MOUSE
Syntaxin 5A
Stx5a
57Annotation score:
H3BJE3H3BJE3_MOUSE
Syntaxin 5A
Stx5a
262Annotation score:
H3BKJ7H3BKJ7_MOUSE
Syntaxin 5A
Stx5a
234Annotation score:
H3BKD6H3BKD6_MOUSE
Syntaxin 5A
Stx5a
77Annotation score:
H3BKP0H3BKP0_MOUSE
Syntaxin 5A
Stx5a
144Annotation score:
H3BL25H3BL25_MOUSE
Syntaxin 5A
Stx5a
101Annotation score:

Sequence cautioni

The sequence AAH04849 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH21883 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91C → S in AAH21883 (PubMed:15489334).Curated1
Sequence conflicti179 – 180LQ → FE in AAF36981 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0201181 – 54Missing in isoform 2. 2 PublicationsAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232709 mRNA Translation: AAF36981.1
AK166087 mRNA Translation: BAE38563.1
BC004849 mRNA Translation: AAH04849.1 Different initiation.
BC021883 mRNA Translation: AAH21883.1 Different initiation.
CCDSiCCDS29542.2 [Q8K1E0-1]
RefSeqiNP_001161271.1, NM_001167799.1 [Q8K1E0-1]
NP_062803.4, NM_019829.4 [Q8K1E0-1]
UniGeneiMm.153061
Mm.454464

Genome annotation databases

EnsembliENSMUST00000073430; ENSMUSP00000073136; ENSMUSG00000010110 [Q8K1E0-1]
ENSMUST00000176381; ENSMUSP00000134854; ENSMUSG00000010110 [Q8K1E0-1]
GeneIDi56389
KEGGimmu:56389
UCSCiuc008gmo.2 mouse [Q8K1E0-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232709 mRNA Translation: AAF36981.1
AK166087 mRNA Translation: BAE38563.1
BC004849 mRNA Translation: AAH04849.1 Different initiation.
BC021883 mRNA Translation: AAH21883.1 Different initiation.
CCDSiCCDS29542.2 [Q8K1E0-1]
RefSeqiNP_001161271.1, NM_001167799.1 [Q8K1E0-1]
NP_062803.4, NM_019829.4 [Q8K1E0-1]
UniGeneiMm.153061
Mm.454464

3D structure databases

ProteinModelPortaliQ8K1E0
SMRiQ8K1E0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207947, 1 interactor
IntActiQ8K1E0, 1 interactor
MINTiQ8K1E0
STRINGi10090.ENSMUSP00000073136

PTM databases

iPTMnetiQ8K1E0
PhosphoSitePlusiQ8K1E0
SwissPalmiQ8K1E0

Proteomic databases

EPDiQ8K1E0
PaxDbiQ8K1E0
PeptideAtlasiQ8K1E0
PRIDEiQ8K1E0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073430; ENSMUSP00000073136; ENSMUSG00000010110 [Q8K1E0-1]
ENSMUST00000176381; ENSMUSP00000134854; ENSMUSG00000010110 [Q8K1E0-1]
GeneIDi56389
KEGGimmu:56389
UCSCiuc008gmo.2 mouse [Q8K1E0-1]

Organism-specific databases

CTDi56389
MGIiMGI:1928483 Stx5a

Phylogenomic databases

eggNOGiKOG0812 Eukaryota
ENOG410Y2MB LUCA
GeneTreeiENSGT00390000000652
HOGENOMiHOG000194860
HOVERGENiHBG001973
InParanoidiQ8K1E0
KOiK08490
OMAiQDRTNEF
PhylomeDBiQ8K1E0
TreeFamiTF315068

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811438 Intra-Golgi traffic

Miscellaneous databases

PROiPR:Q8K1E0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010110 Expressed in 291 organ(s), highest expression level in secondary oocyte
CleanExiMM_STX5A
ExpressionAtlasiQ8K1E0 baseline and differential
GenevisibleiQ8K1E0 MM

Family and domain databases

InterProiView protein in InterPro
IPR010989 SNARE
IPR021538 Syntaxin-5_N
IPR006012 Syntaxin/epimorphin_CS
IPR000727 T_SNARE_dom
PfamiView protein in Pfam
PF05739 SNARE, 1 hit
PF11416 Syntaxin-5_N, 1 hit
SMARTiView protein in SMART
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
PROSITEiView protein in PROSITE
PS00914 SYNTAXIN, 1 hit
PS50192 T_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTX5_MOUSE
AccessioniPrimary (citable) accession number: Q8K1E0
Secondary accession number(s): Q3TM79, Q99J28, Q9JKP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: September 5, 2006
Last modified: November 7, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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