UniProtKB - Q8K183 (PDXK_MOUSE)
Protein
Pyridoxal kinase
Gene
Pdxk
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (By similarity). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions (By similarity).By similarity
Catalytic activityi
- EC:2.7.1.35By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.1.35By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.1.35By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
Activity is increased in the presence of K+or Na+.By similarity
: pyridoxal 5'-phosphate salvage Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxal.By similarityProteins known to be involved in this subpathway in this organism are:
- Pyridoxal kinase (Pdxk)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxal, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.
Pathwayi: pyridoxal 5'-phosphate salvage
This protein is involved in step 1 of the subpathway that synthesizes pyridoxine 5'-phosphate from pyridoxine.By similarityProteins known to be involved in this subpathway in this organism are:
- Pyridoxal kinase (Pdxk)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from pyridoxine, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.
Pathwayi: pyridoxal 5'-phosphate salvage
This protein is involved in step 1 of the subpathway that synthesizes pyridoxamine 5'-phosphate from pyridoxamine.By similarityProteins known to be involved in this subpathway in this organism are:
- Pyridoxal kinase (Pdxk)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxamine 5'-phosphate from pyridoxamine, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | PyridoxalBy similarity | 1 | |
Binding sitei | 47 | Pyridoxal or pyridoxal 5'-phosphateBy similarity | 1 | |
Metal bindingi | 113 | SodiumBy similarity | 1 | |
Binding sitei | 113 | ATPBy similarity | 1 | |
Metal bindingi | 118 | MagnesiumBy similarity | 1 | |
Metal bindingi | 148 | SodiumBy similarity | 1 | |
Metal bindingi | 186 | SodiumBy similarity | 1 | |
Binding sitei | 233 | ATPBy similarity | 1 | |
Active sitei | 235 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 150 – 153 | ATPBy similarity | 4 | |
Nucleotide bindingi | 186 – 187 | ATPBy similarity | 2 | |
Nucleotide bindingi | 226 – 228 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: MGI
- drug binding Source: MGI
- lithium ion binding Source: MGI
- magnesium ion binding Source: MGI
- potassium ion binding Source: MGI
- protein homodimerization activity Source: MGI
- pyridoxal binding Source: MGI
- pyridoxal kinase activity Source: MGI
- pyridoxal phosphate binding Source: MGI
- sodium ion binding Source: MGI
- zinc ion binding Source: MGI
GO - Biological processi
- negative regulation of apoptotic process Source: MGI
- pyridoxal 5'-phosphate salvage Source: MGI
- pyridoxal phosphate biosynthetic process Source: MGI
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Sodium, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation R-MMU-964975, Vitamins B6 activation to pyridoxal phosphate |
UniPathwayi | UPA01068;UER00298 UPA01068;UER00299 UPA01068;UER00300 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351869, Pdxk |
Subcellular locationi
Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213336 | 1 – 312 | Pyridoxal kinaseAdd BLAST | 312 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 59 | PhosphoserineCombined sources | 1 | |
Modified residuei | 164 | PhosphoserineBy similarity | 1 | |
Modified residuei | 213 | PhosphoserineBy similarity | 1 | |
Modified residuei | 285 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8K183 |
jPOSTi | Q8K183 |
MaxQBi | Q8K183 |
PaxDbi | Q8K183 |
PeptideAtlasi | Q8K183 |
PRIDEi | Q8K183 |
PTM databases
iPTMneti | Q8K183 |
PhosphoSitePlusi | Q8K183 |
SwissPalmi | Q8K183 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032788, Expressed in embryo and 348 other tissues |
Genevisiblei | Q8K183, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 229701, 6 interactors |
IntActi | Q8K183, 5 interactors |
MINTi | Q8K183 |
STRINGi | 10090.ENSMUSP00000038540 |
Miscellaneous databases
RNActi | Q8K183, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 234 – 235 | Pyridoxal 5'-phosphate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the pyridoxine kinase family.Curated
Phylogenomic databases
eggNOGi | KOG2599, Eukaryota |
GeneTreei | ENSGT00390000003874 |
HOGENOMi | CLU_046496_1_1_1 |
InParanoidi | Q8K183 |
OMAi | CPNQLEL |
OrthoDBi | 1091630at2759 |
PhylomeDBi | Q8K183 |
TreeFami | TF315004 |
Family and domain databases
CDDi | cd01173, pyridoxal_pyridoxamine_kinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR013749, PM/HMP-P_kinase-1 IPR004625, PyrdxlKinase IPR029056, Ribokinase-like |
PANTHERi | PTHR10534, PTHR10534, 1 hit |
Pfami | View protein in Pfam PF08543, Phos_pyr_kin, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00687, pyridox_kin, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8K183-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGECRVLSI QSHVVRGYVG NRAAMFPLQV LGFEVDAVNS VQFSNHTGYA
60 70 80 90 100
HWKGQVLKSQ ELHELYEGLK VNDVNKYDYV LTGYTRDKSF LAMVVDIVRE
110 120 130 140 150
LKQQNSRLVY VCDPVMGDKW NGEGSMYVPQ DLLPVYRDKV VPVADIITPN
160 170 180 190 200
QFEAELLSGR KIHSQEEAFE VMDMLHCMGP DTVVITSSDL PSSQGSDYLI
210 220 230 240 250
ALGSQRMRKP DGSTVTQRIR MEMRKVEAVF VGTGDLFAAM LLAWTHKHPD
260 270 280 290 300
NLKVACEKTV SAMQHVLQRT IRCAKAEAGE GQKPSPAQLE LRMVQSKRDI
310
EDPEIVVQAT VL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 | S → F in BAC38041 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK039194 mRNA Translation: BAC30274.1 AK080846 mRNA Translation: BAC38041.1 AK145470 mRNA Translation: BAE26454.1 AK166078 mRNA Translation: BAE38559.1 AK166464 mRNA Translation: BAE38792.1 BC027745 mRNA Translation: AAH27745.1 |
CCDSi | CCDS23965.1 |
RefSeqi | NP_742146.1, NM_172134.2 |
Genome annotation databases
Ensembli | ENSMUST00000041616; ENSMUSP00000038540; ENSMUSG00000032788 |
GeneIDi | 216134 |
KEGGi | mmu:216134 |
UCSCi | uc007fxu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK039194 mRNA Translation: BAC30274.1 AK080846 mRNA Translation: BAC38041.1 AK145470 mRNA Translation: BAE26454.1 AK166078 mRNA Translation: BAE38559.1 AK166464 mRNA Translation: BAE38792.1 BC027745 mRNA Translation: AAH27745.1 |
CCDSi | CCDS23965.1 |
RefSeqi | NP_742146.1, NM_172134.2 |
3D structure databases
SMRi | Q8K183 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229701, 6 interactors |
IntActi | Q8K183, 5 interactors |
MINTi | Q8K183 |
STRINGi | 10090.ENSMUSP00000038540 |
PTM databases
iPTMneti | Q8K183 |
PhosphoSitePlusi | Q8K183 |
SwissPalmi | Q8K183 |
Proteomic databases
EPDi | Q8K183 |
jPOSTi | Q8K183 |
MaxQBi | Q8K183 |
PaxDbi | Q8K183 |
PeptideAtlasi | Q8K183 |
PRIDEi | Q8K183 |
Protocols and materials databases
Antibodypediai | 24020, 330 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000041616; ENSMUSP00000038540; ENSMUSG00000032788 |
GeneIDi | 216134 |
KEGGi | mmu:216134 |
UCSCi | uc007fxu.2, mouse |
Organism-specific databases
CTDi | 8566 |
MGIi | MGI:1351869, Pdxk |
Phylogenomic databases
eggNOGi | KOG2599, Eukaryota |
GeneTreei | ENSGT00390000003874 |
HOGENOMi | CLU_046496_1_1_1 |
InParanoidi | Q8K183 |
OMAi | CPNQLEL |
OrthoDBi | 1091630at2759 |
PhylomeDBi | Q8K183 |
TreeFami | TF315004 |
Enzyme and pathway databases
UniPathwayi | UPA01068;UER00298 UPA01068;UER00299 UPA01068;UER00300 |
Reactomei | R-MMU-6798695, Neutrophil degranulation R-MMU-964975, Vitamins B6 activation to pyridoxal phosphate |
Miscellaneous databases
BioGRID-ORCSi | 216134, 5 hits in 18 CRISPR screens |
ChiTaRSi | Pdxk, mouse |
PROi | PR:Q8K183 |
RNActi | Q8K183, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032788, Expressed in embryo and 348 other tissues |
Genevisiblei | Q8K183, MM |
Family and domain databases
CDDi | cd01173, pyridoxal_pyridoxamine_kinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR013749, PM/HMP-P_kinase-1 IPR004625, PyrdxlKinase IPR029056, Ribokinase-like |
PANTHERi | PTHR10534, PTHR10534, 1 hit |
Pfami | View protein in Pfam PF08543, Phos_pyr_kin, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00687, pyridox_kin, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDXK_MOUSE | |
Accessioni | Q8K183Primary (citable) accession number: Q8K183 Secondary accession number(s): Q3TM83, Q8BJQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families