Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CCR4-NOT transcription complex subunit 3

Gene

Cnot3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.2 Publications

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 3
Alternative name(s):
CCR4-associated factor 3
Gene namesi
Name:Cnot3
Synonyms:Not3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385261 Cnot3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983341 – 751CCR4-NOT transcription complex subunit 3Add BLAST751

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei292PhosphothreonineBy similarity1
Modified residuei299PhosphoserineCombined sources1
Modified residuei540PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K0V4
MaxQBiQ8K0V4
PaxDbiQ8K0V4
PeptideAtlasiQ8K0V4
PRIDEiQ8K0V4

PTM databases

iPTMnetiQ8K0V4
PhosphoSitePlusiQ8K0V4

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells and in inner cell mass (ICM) of the blastocyst.1 Publication

Inductioni

Decreased levels in liver and white adipose tissues upon fasting,.1 Publication

Gene expression databases

BgeeiENSMUSG00000035632 Expressed in 194 organ(s), highest expression level in bone marrow
CleanExiMM_CNOT3
ExpressionAtlasiQ8K0V4 baseline and differential
GenevisibleiQ8K0V4 MM

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.1 Publication

Protein-protein interaction databases

BioGridi231295, 18 interactors
DIPiDIP-46844N
IntActiQ8K0V4, 21 interactors
MINTiQ8K0V4
STRINGi10090.ENSMUSP00000039098

Structurei

3D structure databases

ProteinModelPortaliQ8K0V4
SMRiQ8K0V4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni659 – 751Repressor domainBy similarityAdd BLAST93

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi314 – 395Pro-richAdd BLAST82
Compositional biasi396 – 511Ser-richAdd BLAST116

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

eggNOGiKOG2150 Eukaryota
COG5665 LUCA
GeneTreeiENSGT00390000014743
HOGENOMiHOG000184850
HOVERGENiHBG051042
InParanoidiQ8K0V4
KOiK12580
OMAiETQMEKF
OrthoDBiEOG091G040M
PhylomeDBiQ8K0V4
TreeFamiTF321963

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR007282 NOT
IPR007207 Not_N
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.iShow all

Q8K0V4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK
60 70 80 90 100
EIKKLQRLRD QIKTWVASNE IKDKRQLIEN RKLIETQMER FKVVERETKT
110 120 130 140 150
KAYSKEGLGL AQKVDPAQKE KEEVGQWLTN TIDTLNMQVD QFESEVESLS
160 170 180 190 200
VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV RMLETILRML DNDSILVDAI
210 220 230 240 250
RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA TSPPSHSHME
260 270 280 290 300
DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP
310 320 330 340 350
AKNGSKPVHS NQHPQSPAVP PTYPSGPPPT TSALSSTPGN NGASTPAAPT
360 370 380 390 400
SALGPKASPA PSHNSGTPAP YAQAVAPPNA SGPSNAQPRP PSAQPSGGSG
410 420 430 440 450
GGSGGSSSNS NSGTGGGAGK QNGATSYSSV VADSPAEVTL SSSGGSSASS
460 470 480 490 500
QALGPTSGPH NPAPSTSKES STAAPSGAGN VASGSGNNSG GPSLLVPLPV
510 520 530 540 550
NPPSSPTPSF SEAKAAGTLL NGPPQFSTTP EIKAPEPLSS LKSMAERAAI
560 570 580 590 600
SSGIEDPVPT LHLTDRDIIL SSTSAPPTSS QPPLQLSEVN IPLSLGVCPL
610 620 630 640 650
GPVSLTKEQL YQQAMEEAAW HHMPHPSDSE RIRQYLPRNP CPTPPYHHQM
660 670 680 690 700
PPPHSDTVEF YQRLSTETLF FIFYYLEGTK AQYLAAKALK KQSWRFHTKY
710 720 730 740 750
MMWFQRHEEP KTITDEFEQG TYIYFDYEKW GQRKKEGFTF EYRYLEDRDL

Q
Length:751
Mass (Da):81,946
Last modified:October 1, 2002 - v1
Checksum:i47C2A7321EDAB13A
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6YBV1F6YBV1_MOUSE
CCR4-NOT transcription complex subu...
Cnot3
340Annotation score:
F6WUZ2F6WUZ2_MOUSE
CCR4-NOT transcription complex subu...
Cnot3
103Annotation score:
A0A0U1RNN8A0A0U1RNN8_MOUSE
CCR4-NOT transcription complex subu...
Cnot3
100Annotation score:
F6UAW9F6UAW9_MOUSE
CCR4-NOT transcription complex subu...
Cnot3
88Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC030332 mRNA Translation: AAH30332.1
BC053437 mRNA Translation: AAH53437.1
BC141041 mRNA Translation: AAI41042.1
CCDSiCCDS20721.1
RefSeqiNP_666288.1, NM_146176.3
XP_011248803.1, XM_011250501.2
XP_017177635.1, XM_017322146.1
UniGeneiMm.358731

Genome annotation databases

EnsembliENSMUST00000038913; ENSMUSP00000039098; ENSMUSG00000035632
GeneIDi232791
KEGGimmu:232791
UCSCiuc009evl.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCNOT3_MOUSE
AccessioniPrimary (citable) accession number: Q8K0V4
Secondary accession number(s): B2RUA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2002
Last modified: September 12, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again