UniProtKB - Q8K0L9 (ZBT20_MOUSE)
Zinc finger and BTB domain-containing protein 20
Zbtb20
Functioni
May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses (By similarity).
Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 578 – 600 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 606 – 628 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 634 – 656 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 662 – 684 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 715 – 737 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: UniProtKB
GO - Biological processi
- cellular response to glucose stimulus Source: UniProtKB
- lipid homeostasis Source: UniProtKB
- negative regulation of gene expression Source: CACAO
- negative regulation of transcription, DNA-templated Source: CACAO
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of glycolytic process Source: UniProtKB
- positive regulation of interferon-beta production Source: CACAO
- positive regulation of interleukin-6 production Source: CACAO
- positive regulation of lipid biosynthetic process Source: UniProtKB
- positive regulation of tumor necrosis factor production Source: CACAO
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger and BTB domain-containing protein 20Alternative name(s): BTB/POZ domain zinc finger factor HOF Zinc finger protein 288 |
Gene namesi | Name:Zbtb20 Synonyms:Zfp288 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1929213, Zbtb20 |
VEuPathDBi | HostDB:ENSMUSG00000022708 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000325961 | 1 – 741 | Zinc finger and BTB domain-containing protein 20Add BLAST | 741 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 211 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 330 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 330 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 353 | PhosphoserineCombined sources | 1 | |
Modified residuei | 357 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 371 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 690 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 695 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 723 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q8K0L9 |
MaxQBi | Q8K0L9 |
PaxDbi | Q8K0L9 |
PeptideAtlasi | Q8K0L9 |
PRIDEi | Q8K0L9 |
ProteomicsDBi | 275122 [Q8K0L9-1] 275123 [Q8K0L9-2] |
PTM databases
iPTMneti | Q8K0L9 |
PhosphoSitePlusi | Q8K0L9 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022708, Expressed in rostral migratory stream and 320 other tissues |
ExpressionAtlasi | Q8K0L9, baseline and differential |
Genevisiblei | Q8K0L9, MM |
Interactioni
Subunit structurei
Can homodimerize. Binds to DNA.
Protein-protein interaction databases
BioGRIDi | 208015, 19 interactors |
IntActi | Q8K0L9, 2 interactors |
MINTi | Q8K0L9 |
STRINGi | 10090.ENSMUSP00000110339 |
Miscellaneous databases
RNActi | Q8K0L9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 167 | BTBPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 28 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 203 – 235 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 350 – 440 | DisorderedSequence analysisAdd BLAST | 91 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 15 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 208 – 235 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 373 – 389 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 415 – 440 | Polar residuesSequence analysisAdd BLAST | 26 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 578 – 600 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 606 – 628 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 634 – 656 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 662 – 684 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 715 – 737 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158617 |
HOGENOMi | CLU_019055_0_0_1 |
InParanoidi | Q8K0L9 |
OMAi | EQQFMAG |
PhylomeDBi | Q8K0L9 |
TreeFami | TF335684 |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 5 hits |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 5 hits PS50157, ZINC_FINGER_C2H2_2, 5 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLERKKPKTA ENQKASEENE ITQPGGSSAK PALPCLNFEA VLSPAPALIH
60 70 80 90 100
STHSLTNSHA HTGSSDCDIS CKGMTERIHS INLHNFSNSV LETLNEQRNR
110 120 130 140 150
GHFCDVTVRI HGSMLRAHRC VLAAGSPFFQ DKLLLGYSDI EIPSVVSVQS
160 170 180 190 200
VQKLIDFMYS GVLRVSQSEA LQILTAASIL QIKTVIDECT RIVSQNVGDV
210 220 230 240 250
FPGIQDSGQD TPRGTPESGT SGQSSDTESG YLQSHPQHSV DRIYSALYAC
260 270 280 290 300
SMQNGSGERS FYSGAVVSHH ETALGLPRDH HMEDPSWITR IHERSQQMER
310 320 330 340 350
YLSTTPETTH CRKQPRPVRI QTLVGNIHIK QEMEDDYDYY GQQRVQILER
360 370 380 390 400
NESEECTEDT DQAEGTESEP KGESFDSGVS SSIGTEPDSV EQQFGAAAPR
410 420 430 440 450
DGQAEPAQPE QAAEAPAESS AQPNQLEPGA SSPERSNESE MDNTVITVSN
460 470 480 490 500
SSDKGVLQQP SVNTSIGQPL PSTQLYLRQT ETLTSNLRMP LTLTSNTQVI
510 520 530 540 550
GTAGNTYLPA LFTTQPAGSG PKPFLFSLPQ PLTGQQTQFV TVSQPGLSTF
560 570 580 590 600
TAQLPAPQPL ASSAGHSTAS GQGDKKPYEC TLCNKTFTAK QNYVKHMFVH
610 620 630 640 650
TGEKPHQCSI CWRSFSLKDY LIKHMVTHTG VRAYQCSICN KRFTQKSSLN
660 670 680 690 700
VHMRLHRGEK SYECYICKKK FSHKTLLERH VALHSASNGT PPAGTPPGAR
710 720 730 740
AGPPGVVACT EGTTYVCSVC PAKFDQIEQF NDHMRMHVSD G
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CYJ9 | E0CYJ9_MOUSE | Zinc finger and BTB domain-containi... | Zbtb20 | 131 | Annotation score: | ||
E0CX28 | E0CX28_MOUSE | Zinc finger and BTB domain-containi... | Zbtb20 | 63 | Annotation score: | ||
E0CXK1 | E0CXK1_MOUSE | Zinc finger and BTB domain-containi... | Zbtb20 | 75 | Annotation score: | ||
E0CYH3 | E0CYH3_MOUSE | Zinc finger and BTB domain-containi... | Zbtb20 | 138 | Annotation score: | ||
E0CXT9 | E0CXT9_MOUSE | Zinc finger and BTB domain-containi... | Zbtb20 | 60 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | N → D in BAB23755 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 421 | A → D in AAF06015 (PubMed:11744704).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032504 | 1 – 73 | Missing in isoform 2. 2 PublicationsAdd BLAST | 73 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | Q8K0L9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208015, 19 interactors |
IntActi | Q8K0L9, 2 interactors |
MINTi | Q8K0L9 |
STRINGi | 10090.ENSMUSP00000110339 |
PTM databases
iPTMneti | Q8K0L9 |
PhosphoSitePlusi | Q8K0L9 |
Proteomic databases
jPOSTi | Q8K0L9 |
MaxQBi | Q8K0L9 |
PaxDbi | Q8K0L9 |
PeptideAtlasi | Q8K0L9 |
PRIDEi | Q8K0L9 |
ProteomicsDBi | 275122 [Q8K0L9-1] 275123 [Q8K0L9-2] |
Protocols and materials databases
Antibodypediai | 16495, 160 antibodies from 25 providers |
DNASUi | 56490 |
Genome annotation databases
Organism-specific databases
CTDi | 26137 |
MGIi | MGI:1929213, Zbtb20 |
VEuPathDBi | HostDB:ENSMUSG00000022708 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158617 |
HOGENOMi | CLU_019055_0_0_1 |
InParanoidi | Q8K0L9 |
OMAi | EQQFMAG |
PhylomeDBi | Q8K0L9 |
TreeFami | TF335684 |
Miscellaneous databases
BioGRID-ORCSi | 56490, 2 hits in 63 CRISPR screens |
ChiTaRSi | Zbtb20, mouse |
PROi | PR:Q8K0L9 |
RNActi | Q8K0L9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022708, Expressed in rostral migratory stream and 320 other tissues |
ExpressionAtlasi | Q8K0L9, baseline and differential |
Genevisiblei | Q8K0L9, MM |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 5 hits |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 5 hits PS50157, ZINC_FINGER_C2H2_2, 5 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZBT20_MOUSE | |
Accessioni | Q8K0L9Primary (citable) accession number: Q8K0L9 Secondary accession number(s): A6X916, Q9DBD4, Q9QZ87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot