UniProtKB - Q8K0L2 (ENTP8_MOUSE)
Ectonucleoside triphosphate diphosphohydrolase 8
Entpd8
Functioni
Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.
1 PublicationCatalytic activityi
- a ribonucleoside 5'-triphosphate + 2 H2O = a ribonucleoside 5'-phosphate + 2 H+ + 2 phosphate1 PublicationEC:3.6.1.51 Publication
Cofactori
Kineticsi
- KM=13 µM for ATP1 Publication
- KM=41 µM for ADP1 Publication
- KM=47 µM for UTP1 Publication
- KM=171 µM for UDP1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 168 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- 8-oxo-dGTP phosphohydrolase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- dATP phosphohydrolase activity Source: UniProtKB-EC
- dCTP phosphohydrolase activity Source: UniProtKB-EC
- dGTP phosphohydrolase activity Source: UniProtKB-EC
- dTTP phosphohydrolase activity Source: UniProtKB-EC
- dUTP phosphohydrolase activity Source: UniProtKB-EC
- GTP phosphohydrolase activity Source: UniProtKB-EC
- guanosine-diphosphatase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- nucleoside-diphosphatase activity Source: MGI
- nucleoside-triphosphatase activity Source: MGI
- uridine-diphosphatase activity Source: GO_Central
GO - Biological processi
- nucleoside diphosphate biosynthetic process Source: MGI
- nucleoside diphosphate catabolic process Source: GO_Central
- nucleoside monophosphate biosynthetic process Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.1.5, 3474 |
Reactomei | R-MMU-8850843, Phosphate bond hydrolysis by NTPDase proteins |
SABIO-RKi | Q8K0L2 |
Names & Taxonomyi
Protein namesi | Recommended name: Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)Short name: E-NTPDase 8 Short name: NTPDase 8 Short name: NTPDase8 |
Gene namesi | Name:Entpd8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919340, Entpd8 |
VEuPathDBi | HostDB:ENSMUSG00000036813 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 29 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 30 – 473 | ExtracellularSequence analysisAdd BLAST | 444 | |
Transmembranei | 474 – 494 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 495 – 497 | CytoplasmicSequence analysis | 3 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306883 | 1 – 497 | Ectonucleoside triphosphate diphosphohydrolase 8Add BLAST | 497 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 78 ↔ 102 | By similarity | ||
Disulfide bondi | 245 ↔ 294 | By similarity | ||
Glycosylationi | 306 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 331 ↔ 337 | By similarity | ||
Glycosylationi | 365 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 383 ↔ 405 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q8K0L2 |
MaxQBi | Q8K0L2 |
PaxDbi | Q8K0L2 |
PRIDEi | Q8K0L2 |
ProteomicsDBi | 275921 [Q8K0L2-1] 275922 [Q8K0L2-2] |
PTM databases
GlyGeni | Q8K0L2, 2 sites |
iPTMneti | Q8K0L2 |
PhosphoSitePlusi | Q8K0L2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000036813, Expressed in duodenum and 64 other tissues |
ExpressionAtlasi | Q8K0L2, baseline and differential |
Genevisiblei | Q8K0L2, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000110022 |
Miscellaneous databases
RNActi | Q8K0L2, protein |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1386, Eukaryota |
GeneTreei | ENSGT01020000230463 |
HOGENOMi | CLU_010246_2_3_1 |
InParanoidi | Q8K0L2 |
OMAi | SNVDVGW |
OrthoDBi | 1337265at2759 |
PhylomeDBi | Q8K0L2 |
TreeFami | TF332859 |
Family and domain databases
InterProi | View protein in InterPro IPR000407, GDA1_CD39_NTPase |
PANTHERi | PTHR11782, PTHR11782, 1 hit |
Pfami | View protein in Pfam PF01150, GDA1_CD39, 1 hit |
PROSITEi | View protein in PROSITE PS01238, GDA1_CD39_NTPASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG
60 70 80 90 100
SSHTSLFVYQ WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK
110 120 130 140 150
SCLEEALALI PQAQHPETPT FLGSTAGMRL LSQKNSSQAR DILAAVSQTL
160 170 180 190 200
SKSPVDFWGA KILAGQDEGA FGWITINYVL GMLLKYSSGQ WILPEEGMLV
210 220 230 240 250
GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT HSYLCFGRDQ
260 270 280 290 300
ILNRLLAKLA QDRLSSQVAP VRHPCYHSGY QAILPLSSLY DSPCIHTTDS
310 320 330 340 350
LNHTQNLTVE GTGDPGNCVV ALRSLFNFSS CKGQKDCAFN GIYQPPVHGQ
360 370 380 390 400
FYAFSNFYYT FHFLNLTSRQ SLNTVNDTVW KFCQKPWKLV EVSYPGQERW
410 420 430 440 450
LRDYCASGLY ILVLLLEGYK FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL
460 470 480 490
TGMIPAEAPT HWRAQSYSIW TAGVVFAVLT LVAILGAAAI QIFWTQD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YY80 | A0A0A6YY80_MOUSE | Ectonucleoside triphosphate diphosp... | Entpd8 | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 437 | D → N in AAH31143 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028560 | 263 – 265 | Missing in isoform 2. Curated | 3 | |
Alternative sequenceiVSP_028561 | 353 – 389 | Missing in isoform 2. CuratedAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY364442 mRNA Translation: AAQ84519.1 AL732585 Genomic DNA No translation available. BC031143 mRNA Translation: AAH31143.2 |
CCDSi | CCDS15748.1 [Q8K0L2-1] |
RefSeqi | NP_082369.1, NM_028093.1 [Q8K0L2-1] XP_006498425.1, XM_006498362.3 |
Genome annotation databases
Ensembli | ENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813 [Q8K0L2-1] ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813 [Q8K0L2-2] ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813 [Q8K0L2-1] |
GeneIDi | 72090 |
KEGGi | mmu:72090 |
UCSCi | uc008iqf.1, mouse [Q8K0L2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY364442 mRNA Translation: AAQ84519.1 AL732585 Genomic DNA No translation available. BC031143 mRNA Translation: AAH31143.2 |
CCDSi | CCDS15748.1 [Q8K0L2-1] |
RefSeqi | NP_082369.1, NM_028093.1 [Q8K0L2-1] XP_006498425.1, XM_006498362.3 |
3D structure databases
SMRi | Q8K0L2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000110022 |
PTM databases
GlyGeni | Q8K0L2, 2 sites |
iPTMneti | Q8K0L2 |
PhosphoSitePlusi | Q8K0L2 |
Proteomic databases
jPOSTi | Q8K0L2 |
MaxQBi | Q8K0L2 |
PaxDbi | Q8K0L2 |
PRIDEi | Q8K0L2 |
ProteomicsDBi | 275921 [Q8K0L2-1] 275922 [Q8K0L2-2] |
Protocols and materials databases
Antibodypediai | 19016, 156 antibodies from 20 providers |
Genome annotation databases
Ensembli | ENSMUST00000044078; ENSMUSP00000040628; ENSMUSG00000036813 [Q8K0L2-1] ENSMUST00000114376; ENSMUSP00000110017; ENSMUSG00000036813 [Q8K0L2-2] ENSMUST00000114380; ENSMUSP00000110022; ENSMUSG00000036813 [Q8K0L2-1] |
GeneIDi | 72090 |
KEGGi | mmu:72090 |
UCSCi | uc008iqf.1, mouse [Q8K0L2-1] |
Organism-specific databases
CTDi | 377841 |
MGIi | MGI:1919340, Entpd8 |
VEuPathDBi | HostDB:ENSMUSG00000036813 |
Phylogenomic databases
eggNOGi | KOG1386, Eukaryota |
GeneTreei | ENSGT01020000230463 |
HOGENOMi | CLU_010246_2_3_1 |
InParanoidi | Q8K0L2 |
OMAi | SNVDVGW |
OrthoDBi | 1337265at2759 |
PhylomeDBi | Q8K0L2 |
TreeFami | TF332859 |
Enzyme and pathway databases
BRENDAi | 3.6.1.5, 3474 |
Reactomei | R-MMU-8850843, Phosphate bond hydrolysis by NTPDase proteins |
SABIO-RKi | Q8K0L2 |
Miscellaneous databases
BioGRID-ORCSi | 72090, 1 hit in 65 CRISPR screens |
ChiTaRSi | Cant1, mouse |
PROi | PR:Q8K0L2 |
RNActi | Q8K0L2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036813, Expressed in duodenum and 64 other tissues |
ExpressionAtlasi | Q8K0L2, baseline and differential |
Genevisiblei | Q8K0L2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000407, GDA1_CD39_NTPase |
PANTHERi | PTHR11782, PTHR11782, 1 hit |
Pfami | View protein in Pfam PF01150, GDA1_CD39, 1 hit |
PROSITEi | View protein in PROSITE PS01238, GDA1_CD39_NTPASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTP8_MOUSE | |
Accessioni | Q8K0L2Primary (citable) accession number: Q8K0L2 Secondary accession number(s): A2AJ99, Q6UQ22 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 2, 2007 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families