UniProtKB - Q8K0E8 (FIBB_MOUSE)
Protein
Fibrinogen beta chain
Gene
Fgb
Organism
Mus musculus (Mouse)
Status
Functioni
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.By similarity
GO - Molecular functioni
- cell adhesion molecule binding Source: Ensembl
- chaperone binding Source: MGI
- signaling receptor binding Source: GO_Central
- structural molecule activity Source: MGI
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- blood coagulation, fibrin clot formation Source: MGI
- cell adhesion Source: GO_Central
- cell-matrix adhesion Source: MGI
- cellular protein-containing complex assembly Source: MGI
- cellular response to interleukin-1 Source: Ensembl
- cellular response to leptin stimulus Source: Ensembl
- fibrinolysis Source: MGI
- induction of bacterial agglutination Source: MGI
- innate immune response Source: UniProtKB-KW
- negative regulation of endothelial cell apoptotic process Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- plasminogen activation Source: MGI
- platelet aggregation Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of exocytosis Source: MGI
- positive regulation of heterotypic cell-cell adhesion Source: MGI
- positive regulation of peptide hormone secretion Source: MGI
- positive regulation of protein secretion Source: MGI
- positive regulation of vasoconstriction Source: MGI
- protein polymerization Source: MGI
- response to calcium ion Source: MGI
Keywordsi
Biological process | Adaptive immunity, Blood coagulation, Hemostasis, Immunity, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-216083, Integrin cell surface interactions R-MMU-354192, Integrin signaling R-MMU-354194, GRB2:SOS provides linkage to MAPK signaling for Integrins R-MMU-372708, p130Cas linkage to MAPK signaling for integrins R-MMU-5674135, MAP2K and MAPK activation R-MMU-5686938, Regulation of TLR by endogenous ligand |
Names & Taxonomyi
Protein namesi | Recommended name: Fibrinogen beta chainCleaved into the following 2 chains: |
Gene namesi | Name:Fgb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99501, Fgb |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- blood microparticle Source: MGI
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular space Source: MGI
- fibrinogen complex Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
Other locations
- cell cortex Source: MGI
- cell surface Source: MGI
- cytoplasm Source: MGI
- platelet alpha granule Source: MGI
- synapse Source: SynGO
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | By similarityAdd BLAST | 19 | |
PeptideiPRO_0000009078 | 20 – 34 | Fibrinopeptide BBy similarityAdd BLAST | 15 | |
ChainiPRO_0000009077 | 35 – 481 | Fibrinogen beta chainAdd BLAST | 447 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 213 | Interchain (with alpha chain)PROSITE-ProRule annotation | ||
Disulfide bondi | 217 | Interchain (with gamma chain)PROSITE-ProRule annotation | ||
Disulfide bondi | 221 ↔ 306 | PROSITE-ProRule annotation | ||
Disulfide bondi | 231 ↔ 260 | PROSITE-ProRule annotation | ||
Glycosylationi | 384 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 414 ↔ 427 | PROSITE-ProRule annotation |
Post-translational modificationi
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 34 – 35 | Cleavage; by thrombin; to release fibrinopeptide BBy similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-3332 |
jPOSTi | Q8K0E8 |
MaxQBi | Q8K0E8 |
PaxDbi | Q8K0E8 |
PeptideAtlasi | Q8K0E8 |
PRIDEi | Q8K0E8 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00279079 Q8K0E8 |
PTM databases
GlyConnecti | 2312, 1 N-Linked glycan (1 site) |
GlyGeni | Q8K0E8, 1 site |
iPTMneti | Q8K0E8 |
PhosphoSitePlusi | Q8K0E8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000033831, Expressed in liver and 96 other tissues |
Genevisiblei | Q8K0E8, MM |
Interactioni
Subunit structurei
Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain (By similarity).
By similarityGO - Molecular functioni
- cell adhesion molecule binding Source: Ensembl
- chaperone binding Source: MGI
- signaling receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 225325, 13 interactors |
ComplexPortali | CPX-1916, Fibrinogen |
IntActi | Q8K0E8, 8 interactors |
MINTi | Q8K0E8 |
STRINGi | 10090.ENSMUSP00000039472 |
Miscellaneous databases
RNActi | Q8K0E8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 222 – 478 | Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST | 257 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 37 | Beta-chain polymerization, binding distal domain of another fibrinBy similarity | 3 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 149 – 213 | Sequence analysisAdd BLAST | 65 |
Domaini
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure (By similarity).By similarity
Keywords - Domaini
Coiled coil, SignalPhylogenomic databases
eggNOGi | KOG2579, Eukaryota |
GeneTreei | ENSGT00940000158122 |
HOGENOMi | CLU_038628_13_0_1 |
InParanoidi | Q8K0E8 |
OMAi | VYPDAGG |
OrthoDBi | 357340at2759 |
PhylomeDBi | Q8K0E8 |
TreeFami | TF336658 |
Family and domain databases
CDDi | cd00087, FReD, 1 hit |
InterProi | View protein in InterPro IPR036056, Fibrinogen-like_C IPR002181, Fibrinogen_a/b/g_C_dom IPR012290, Fibrinogen_a/b/g_coil_dom IPR037580, Fibrinogen_beta IPR020837, Fibrinogen_CS |
PANTHERi | PTHR19143:SF332, PTHR19143:SF332, 1 hit |
Pfami | View protein in Pfam PF08702, Fib_alpha, 1 hit PF00147, Fibrinogen_C, 1 hit |
SMARTi | View protein in SMART SM00186, FBG, 1 hit SM01212, Fib_alpha, 1 hit |
SUPFAMi | SSF56496, SSF56496, 1 hit |
PROSITEi | View protein in PROSITE PS00514, FIBRINOGEN_C_1, 1 hit PS51406, FIBRINOGEN_C_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8K0E8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRHLWLLLLL CVFSVQTQAA DDDYDEPTDS LDARGHRPVD RRKEEPPSLR
60 70 80 90 100
PAPPPISGGG YRARPAKATA NQKKVERRPP DAGGCLHADT DMGVLCPTGC
110 120 130 140 150
TLQQTLLNQE RPIKSSIAEL NNNIQSVSDT SSVTFQYLTL LKDMWKKKQA
160 170 180 190 200
QVKENENVIN EYSSILEDQR LYIDETVNDN IPLNLRVLRS ILEDLRSKIQ
210 220 230 240 250
KLESDISAQM EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE TSEMYLIQPD
260 270 280 290 300
TSIKPYRVYC DMKTENGGWT VIQNRQDGSV DFGRKWDPYK KGFGNIATNE
310 320 330 340 350
DAKKYCGLPG EYWLGNDKIS QLTRMGPTEL LIEMEDWKGD KVKAHYGGFT
360 370 380 390 400
VQNEASKYQV SVNKYKGTAG NALMDGASQL VGENRTMTIH NGMFFSTYDR
410 420 430 440 450
DNDGWVTTDP RKQCSKEDGG GWWYNRCHAA NPNGRYYWGG LYSWDMSKHG
460 470 480
TDDGVVWMNW KGSWYSMRRM SMKIRPFFPQ Q
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC031715 mRNA Translation: AAH31715.1 AF413205 mRNA Translation: AAL02225.1 |
CCDSi | CCDS17432.1 |
RefSeqi | NP_862897.1, NM_181849.3 |
Genome annotation databases
Ensembli | ENSMUST00000048246; ENSMUSP00000039472; ENSMUSG00000033831 |
GeneIDi | 110135 |
KEGGi | mmu:110135 |
UCSCi | uc008pph.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC031715 mRNA Translation: AAH31715.1 AF413205 mRNA Translation: AAL02225.1 |
CCDSi | CCDS17432.1 |
RefSeqi | NP_862897.1, NM_181849.3 |
3D structure databases
SMRi | Q8K0E8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225325, 13 interactors |
ComplexPortali | CPX-1916, Fibrinogen |
IntActi | Q8K0E8, 8 interactors |
MINTi | Q8K0E8 |
STRINGi | 10090.ENSMUSP00000039472 |
PTM databases
GlyConnecti | 2312, 1 N-Linked glycan (1 site) |
GlyGeni | Q8K0E8, 1 site |
iPTMneti | Q8K0E8 |
PhosphoSitePlusi | Q8K0E8 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00279079 Q8K0E8 |
Proteomic databases
CPTACi | non-CPTAC-3332 |
jPOSTi | Q8K0E8 |
MaxQBi | Q8K0E8 |
PaxDbi | Q8K0E8 |
PeptideAtlasi | Q8K0E8 |
PRIDEi | Q8K0E8 |
Protocols and materials databases
Antibodypediai | 855, 570 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000048246; ENSMUSP00000039472; ENSMUSG00000033831 |
GeneIDi | 110135 |
KEGGi | mmu:110135 |
UCSCi | uc008pph.1, mouse |
Organism-specific databases
CTDi | 2244 |
MGIi | MGI:99501, Fgb |
Phylogenomic databases
eggNOGi | KOG2579, Eukaryota |
GeneTreei | ENSGT00940000158122 |
HOGENOMi | CLU_038628_13_0_1 |
InParanoidi | Q8K0E8 |
OMAi | VYPDAGG |
OrthoDBi | 357340at2759 |
PhylomeDBi | Q8K0E8 |
TreeFami | TF336658 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-216083, Integrin cell surface interactions R-MMU-354192, Integrin signaling R-MMU-354194, GRB2:SOS provides linkage to MAPK signaling for Integrins R-MMU-372708, p130Cas linkage to MAPK signaling for integrins R-MMU-5674135, MAP2K and MAPK activation R-MMU-5686938, Regulation of TLR by endogenous ligand |
Miscellaneous databases
BioGRID-ORCSi | 110135, 1 hit in 17 CRISPR screens |
ChiTaRSi | Fgb, mouse |
PROi | PR:Q8K0E8 |
RNActi | Q8K0E8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033831, Expressed in liver and 96 other tissues |
Genevisiblei | Q8K0E8, MM |
Family and domain databases
CDDi | cd00087, FReD, 1 hit |
InterProi | View protein in InterPro IPR036056, Fibrinogen-like_C IPR002181, Fibrinogen_a/b/g_C_dom IPR012290, Fibrinogen_a/b/g_coil_dom IPR037580, Fibrinogen_beta IPR020837, Fibrinogen_CS |
PANTHERi | PTHR19143:SF332, PTHR19143:SF332, 1 hit |
Pfami | View protein in Pfam PF08702, Fib_alpha, 1 hit PF00147, Fibrinogen_C, 1 hit |
SMARTi | View protein in SMART SM00186, FBG, 1 hit SM01212, Fib_alpha, 1 hit |
SUPFAMi | SSF56496, SSF56496, 1 hit |
PROSITEi | View protein in PROSITE PS00514, FIBRINOGEN_C_1, 1 hit PS51406, FIBRINOGEN_C_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FIBB_MOUSE | |
Accessioni | Q8K0E8Primary (citable) accession number: Q8K0E8 Secondary accession number(s): Q91ZP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot