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Protein

Hyaluronan-binding protein 2

Gene

Habp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei360Charge relay systemBy similarity1
Active sitei409Charge relay systemBy similarity1
Active sitei507Charge relay systemBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.033

Names & Taxonomyi

Protein namesi
Gene namesi
Name:Habp2
Synonyms:Phbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1196378 Habp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000002790324 – 311Hyaluronan-binding protein 2 50 kDa heavy chainAdd BLAST288
ChainiPRO_000002790427 – 311Hyaluronan-binding protein 2 50 kDa heavy chain alternate formAdd BLAST285
ChainiPRO_0000027905312 – 558Hyaluronan-binding protein 2 27 kDa light chainAdd BLAST247
ChainiPRO_0000027906318 – 558Hyaluronan-binding protein 2 27 kDa light chain alternate formAdd BLAST241

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi75 ↔ 86By similarity
Disulfide bondi80 ↔ 95By similarity
Disulfide bondi97 ↔ 106By similarity
Disulfide bondi113 ↔ 123By similarity
Disulfide bondi118 ↔ 134By similarity
Disulfide bondi136 ↔ 145By similarity
Disulfide bondi152 ↔ 163By similarity
Disulfide bondi157 ↔ 174By similarity
Disulfide bondi176 ↔ 185By similarity
Disulfide bondi192 ↔ 274By similarity
Disulfide bondi213 ↔ 255By similarity
Disulfide bondi244 ↔ 269By similarity
Disulfide bondi299 ↔ 433Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi345 ↔ 361By similarity
Disulfide bondi445 ↔ 513By similarity
Disulfide bondi475 ↔ 491By similarity
Disulfide bondi503 ↔ 531By similarity

Post-translational modificationi

Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-311 or Lys-317 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Arg-168 or Arg-169 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-478 to give rise to inactive 17 kDa and 8 kDa fragments.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei167 – 168Cleavage2
Sitei168 – 169Cleavage2
Sitei478 – 479Cleavage2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiQ8K0D2

PTM databases

PhosphoSitePlusiQ8K0D2

Expressioni

Tissue specificityi

Liver and kidney.1 Publication

Gene expression databases

CleanExiMM_HABP2

Interactioni

Subunit structurei

Heterodimer; disulfide-linked. Heterodimer of a 50 kDa heavy and a 27 kDa light chain linked by a disulfide bond.

Structurei

3D structure databases

ProteinModelPortaliQ8K0D2
SMRiQ8K0D2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 107EGF-like 1PROSITE-ProRule annotationAdd BLAST37
Domaini109 – 146EGF-like 2PROSITE-ProRule annotationAdd BLAST38
Domaini148 – 186EGF-like 3PROSITE-ProRule annotationAdd BLAST39
Domaini191 – 274KringlePROSITE-ProRule annotationAdd BLAST84
Domaini312 – 553Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000237314
HOVERGENiHBG106385
InParanoidiQ8K0D2
KOiK08648
PhylomeDBiQ8K0D2

Family and domain databases

CDDicd00108 KR, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 3 hits
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8K0D2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFVRMLVFRV LLLIALVGKS VIGLSLMSFI APPDPDWTPD DYYYSYEQSS
60 70 80 90 100
PDEDPSVTQT TPENPDWYYE DDDPCQSNPC EHGGDCIIRG DTFSCSCPAP
110 120 130 140 150
FSGSRCQTAQ NKCKDNPCVH GDCLITQKHP YYRCACKYPY TGPDCSKVLP
160 170 180 190 200
ACRPNPCQNG GVCSRHRRRS RFTCACPDQY KGKFCEIGPD DCYVGDGYSY
210 220 230 240 250
RGKVSKTVNQ NPCLYWNSHL LLQETYNMFM EDAETHGIAE HNFCRNPDGD
260 270 280 290 300
HKPWCFVKVN SEKVKWEYCD VTVCPVPDTP NPVESLLEPV MELPGFESCG
310 320 330 340 350
KTEVAEHAVK RIYGGFKSTA GKHPWQVSLQ TSLPLTTSMP QGHFCGGALI
360 370 380 390 400
HPCWVLTAAH CTDINTKHLK VVLGDQDLKK TESHEQTFRV EKILKYSQYN
410 420 430 440 450
ERDEIPHNDI ALLKLKPVGG HCALESRYVK TVCLPSDPFP SGTECHISGW
460 470 480 490 500
GVTETGEGSR QLLDAKVKLI ANPLCNSRQL YDHTIDDSMI CAGNLQKPGS
510 520 530 540 550
DTCQGDSGGP LTCEKDGTYY VYGIVSWGQE CGKKPGVYTQ VTKFLNWIKT

TMHREAGL
Length:558
Mass (Da):62,357
Last modified:August 30, 2005 - v2
Checksum:i0580CB38F6708E41
GO
Isoform 2 (identifier: Q8K0D2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-72: Missing.

Note: No experimental confirmation available.
Show »
Length:521
Mass (Da):57,860
Checksum:i184D3F7935B202CD
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3V1J8Q3V1J8_MOUSE
Hyaluronan-binding protein 2
Habp2
517Annotation score:
E9Q092E9Q092_MOUSE
Hyaluronan-binding protein 2
Habp2
549Annotation score:
E9QM92E9QM92_MOUSE
Hyaluronan-binding protein 2
Habp2
554Annotation score:
E9Q0C5E9Q0C5_MOUSE
Hyaluronan-binding protein 2
Habp2
274Annotation score:
F7B276F7B276_MOUSE
Hyaluronan-binding protein 2
Habp2
220Annotation score:
F7D2A6F7D2A6_MOUSE
Hyaluronan-binding protein 2
Habp2
124Annotation score:

Sequence cautioni

The sequence AAH31775 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01539536 – 72Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031775 mRNA Translation: AAH31775.1 Different initiation.
PIRiJC5878
RefSeqiNP_666213.1, NM_146101.2
UniGeneiMm.25791

Genome annotation databases

GeneIDi226243
KEGGimmu:226243
UCSCiuc008hyt.1 mouse [Q8K0D2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031775 mRNA Translation: AAH31775.1 Different initiation.
PIRiJC5878
RefSeqiNP_666213.1, NM_146101.2
UniGeneiMm.25791

3D structure databases

ProteinModelPortaliQ8K0D2
SMRiQ8K0D2
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.033

PTM databases

PhosphoSitePlusiQ8K0D2

Proteomic databases

PRIDEiQ8K0D2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi226243
KEGGimmu:226243
UCSCiuc008hyt.1 mouse [Q8K0D2-1]

Organism-specific databases

CTDi3026
MGIiMGI:1196378 Habp2

Phylogenomic databases

HOGENOMiHOG000237314
HOVERGENiHBG106385
InParanoidiQ8K0D2
KOiK08648
PhylomeDBiQ8K0D2

Miscellaneous databases

ChiTaRSiHabp2 mouse
PROiPR:Q8K0D2
SOURCEiSearch...

Gene expression databases

CleanExiMM_HABP2

Family and domain databases

CDDicd00108 KR, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 3 hits
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHABP2_MOUSE
AccessioniPrimary (citable) accession number: Q8K0D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 7, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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