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Protein

Lanosterol 14-alpha demethylase

Gene

Cyp51a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.By similarity

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi449Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-191273 Cholesterol biosynthesis
R-MMU-211976 Endogenous sterols
UniPathwayiUPA00770; UER00754

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.70)
Short name:
LDM
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name:
Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene namesi
Name:Cyp51a1
Synonyms:Cyp51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:106040 Cyp51

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3774294

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003767961 – 503Lanosterol 14-alpha demethylaseAdd BLAST503

Proteomic databases

EPDiQ8K0C4
MaxQBiQ8K0C4
PaxDbiQ8K0C4
PeptideAtlasiQ8K0C4
PRIDEiQ8K0C4

PTM databases

iPTMnetiQ8K0C4
PhosphoSitePlusiQ8K0C4
SwissPalmiQ8K0C4

Expressioni

Gene expression databases

BgeeiENSMUSG00000001467
GenevisibleiQ8K0C4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001507

Structurei

3D structure databases

ProteinModelPortaliQ8K0C4
SMRiQ8K0C4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00800000124060
HOGENOMiHOG000042780
InParanoidiQ8K0C4
KOiK05917
OMAiAPIHSIM
OrthoDBiEOG091G04XH
PhylomeDBiQ8K0C4
TreeFamiTF105091

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K0C4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLGLLQSG GWVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH
60 70 80 90 100
MVQLPAGAKS PPHIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM
110 120 130 140 150
VGKTFTYLLG SDAAALLFNS KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA
160 170 180 190 200
IFLEQKKIIK SGLNIAHFKQ YVPIIEKEAK EYFQSWGESG ERNVFEALSE
210 220 230 240 250
LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFTHAAWL LPAWLPLPSF
260 270 280 290 300
RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDEE
310 320 330 340 350
ISGMLIGLLL AGQHTSSTTS AWMGFFLAKD KPLQEKCYLE QKAVCGEDLP
360 370 380 390 400
PLTYDQLKDL NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ
410 420 430 440 450
VCVSPTVNQR LKDSWAERLD FNPDRYLQDN PASGEKFAYV PFGAGRHRCV
460 470 480 490 500
GENFAYVQIK TIWSTMLRLY EFDLINGYFP TVNYTTMIHT PENPVIRYKR

RSK
Length:503
Mass (Da):56,776
Last modified:October 1, 2002 - v1
Checksum:i6A4BBA350F1D85C7
GO

Sequence cautioni

The sequence AAF73986 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19E → A in AAF74562 (PubMed:10864439).Curated1
Sequence conflicti98F → L in AAF74562 (PubMed:10864439).Curated1
Sequence conflicti98F → L in AAF73986 (PubMed:10653123).Curated1
Sequence conflicti235T → A in BAC27231 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF204804
, AF204796, AF204797, AF204798, AF204799, AF204800, AF204801, AF204802, AF204803 Genomic DNA Translation: AAF74562.1
AF166266 mRNA Translation: AAF73986.1 Different initiation.
AK028355 mRNA Translation: BAC25900.1
AK028815 mRNA Translation: BAC26134.1
AK031059 mRNA Translation: BAC27231.1
AK076983 mRNA Translation: BAC36548.1
CH466600 Genomic DNA Translation: EDL14627.1
BC031813 mRNA Translation: AAH31813.1
CCDSiCCDS19071.1
RefSeqiNP_064394.2, NM_020010.2
UniGeneiMm.140158

Genome annotation databases

EnsembliENSMUST00000001507; ENSMUSP00000001507; ENSMUSG00000001467
GeneIDi13121
KEGGimmu:13121
UCSCiuc008wie.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCP51A_MOUSE
AccessioniPrimary (citable) accession number: Q8K0C4
Secondary accession number(s): Q8BSQ7, Q9JIP8, Q9JIY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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