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Protein

Probable lysosomal cobalamin transporter

Gene

Lmbrd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors (By similarity).By similarity

GO - Molecular functioni

  • cobalamin binding Source: UniProtKB-KW
  • insulin receptor binding Source: MGI

GO - Biological processi

Keywordsi

Biological processTransport
LigandCobalamin, Cobalt

Enzyme and pathway databases

ReactomeiR-MMU-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lysosomal cobalamin transporter
Alternative name(s):
LMBR1 domain-containing protein 1
Protein N90b
Gene namesi
Name:Lmbrd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915671 Lmbrd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7ExtracellularSequence analysis7
Transmembranei8 – 28Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini29 – 47CytoplasmicSequence analysisAdd BLAST19
Transmembranei48 – 68Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini69 – 97ExtracellularSequence analysisAdd BLAST29
Transmembranei98 – 118Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini119 – 141CytoplasmicSequence analysisAdd BLAST23
Transmembranei142 – 162Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini163 – 185ExtracellularSequence analysisAdd BLAST23
Transmembranei186 – 206Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini207 – 302CytoplasmicSequence analysisAdd BLAST96
Transmembranei303 – 323Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini324 – 361ExtracellularSequence analysisAdd BLAST38
Transmembranei362 – 382Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini383 – 405CytoplasmicSequence analysisAdd BLAST23
Transmembranei406 – 426Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini427 – 483ExtracellularSequence analysisAdd BLAST57
Transmembranei484 – 504Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini505 – 537CytoplasmicSequence analysisAdd BLAST33

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002605171 – 537Probable lysosomal cobalamin transporterAdd BLAST537

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei235PhosphothreonineCombined sources1
Glycosylationi344N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi445N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi454N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei525PhosphoserineCombined sources1
Modified residuei528PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8K0B2
PeptideAtlasiQ8K0B2
PRIDEiQ8K0B2

PTM databases

iPTMnetiQ8K0B2
PhosphoSitePlusiQ8K0B2
SwissPalmiQ8K0B2

Expressioni

Gene expression databases

BgeeiENSMUSG00000073725 Expressed in 271 organ(s), highest expression level in saccule of membranous labyrinth
CleanExiMM_LMBRD1
ExpressionAtlasiQ8K0B2 baseline and differential
GenevisibleiQ8K0B2 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092672

Family & Domainsi

Sequence similaritiesi

Belongs to the LIMR family. LMBRD1 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFHV Eukaryota
ENOG410XPKG LUCA
GeneTreeiENSGT00390000002581
HOGENOMiHOG000068018
HOVERGENiHBG059142
InParanoidiQ8K0B2
KOiK14617
OMAiLNVPNNK
OrthoDBiEOG091G0BP6
PhylomeDBiQ8K0B2
TreeFamiTF329170

Family and domain databases

InterProiView protein in InterPro
IPR006876 LMBR1-like_membr_prot
PfamiView protein in Pfam
PF04791 LMBR1, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8K0B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS
60 70 80 90 100
LAVALITSAL LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT
110 120 130 140 150
LYSVILFCVF FWIPFVYFYY EEKDEDDASK CTQIKTALKY TLGFVVICAL
160 170 180 190 200
LLLVGAFVPL HLPNNNNSTE WEKVKLLFED LGTGQGLAAL SFSISSLTLI
210 220 230 240 250
GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE VEQHIQTIRS
260 270 280 290 300
KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP
310 320 330 340 350
LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN
360 370 380 390 400
MLLPLLQTVF PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG
410 420 430 440 450
RTRPQALLFL CMILLLIVLH TSYMIYSLAP QYVMYGSQNY LIESNITSDA
460 470 480 490 500
HKGNSTLAVP KRCDADAPKD QCTVTRTYIF LHKFWFFSAA YYFGNWAFLV
510 520 530
VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA
Length:537
Mass (Da):61,062
Last modified:October 1, 2002 - v1
Checksum:i7AAFF431479752F0
GO
Isoform 2 (identifier: Q8K0B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     19-20: LL → MQ

Note: No experimental confirmation available.
Show »
Length:519
Mass (Da):59,264
Checksum:i5F1EB67EA4D94D32
GO
Isoform 3 (identifier: Q8K0B2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:467
Mass (Da):53,440
Checksum:iBC4647DF0C55E55A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WPS2A0A087WPS2_MOUSE
Probable lysosomal cobalamin transp...
Lmbrd1
143Annotation score:
A0A087WS60A0A087WS60_MOUSE
Probable lysosomal cobalamin transp...
Lmbrd1
161Annotation score:
A0A087WR85A0A087WR85_MOUSE
Probable lysosomal cobalamin transp...
Lmbrd1
143Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62P → L in BAC27926 (PubMed:16141072).Curated1
Sequence conflicti173K → Q in AAL38052 (Ref. 1) Curated1
Sequence conflicti193S → P in AAL38052 (Ref. 1) Curated1
Sequence conflicti386G → D in BAE31002 (PubMed:16141072).Curated1
Sequence conflicti386G → D in BAE31829 (PubMed:16141072).Curated1
Sequence conflicti496W → R in BAB22556 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0216311 – 70Missing in isoform 3. 3 PublicationsAdd BLAST70
Alternative sequenceiVSP_0216321 – 18Missing in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_02163319 – 20LL → MQ in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061989 mRNA Translation: AAL38052.1
AK003084 mRNA Translation: BAB22556.1
AK032560 mRNA Translation: BAC27926.1
AK152170 mRNA Translation: BAE31002.1
AK153237 mRNA Translation: BAE31829.1
AK158982 mRNA Translation: BAE34756.1
AK167080 mRNA Translation: BAE39240.1
BC025579 mRNA Translation: AAH25579.1
BC027774 mRNA Translation: AAH27774.1
BC031902 mRNA Translation: AAH31902.1
BC039053 mRNA Translation: AAH39053.1
CCDSiCCDS35528.1 [Q8K0B2-1]
CCDS78556.1 [Q8K0B2-3]
RefSeqiNP_001297412.1, NM_001310483.1 [Q8K0B2-3]
NP_080995.2, NM_026719.2 [Q8K0B2-1]
UniGeneiMm.336563

Genome annotation databases

EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725 [Q8K0B2-3]
ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725 [Q8K0B2-1]
GeneIDi68421
KEGGimmu:68421
UCSCiuc007amt.1 mouse [Q8K0B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061989 mRNA Translation: AAL38052.1
AK003084 mRNA Translation: BAB22556.1
AK032560 mRNA Translation: BAC27926.1
AK152170 mRNA Translation: BAE31002.1
AK153237 mRNA Translation: BAE31829.1
AK158982 mRNA Translation: BAE34756.1
AK167080 mRNA Translation: BAE39240.1
BC025579 mRNA Translation: AAH25579.1
BC027774 mRNA Translation: AAH27774.1
BC031902 mRNA Translation: AAH31902.1
BC039053 mRNA Translation: AAH39053.1
CCDSiCCDS35528.1 [Q8K0B2-1]
CCDS78556.1 [Q8K0B2-3]
RefSeqiNP_001297412.1, NM_001310483.1 [Q8K0B2-3]
NP_080995.2, NM_026719.2 [Q8K0B2-1]
UniGeneiMm.336563

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092672

PTM databases

iPTMnetiQ8K0B2
PhosphoSitePlusiQ8K0B2
SwissPalmiQ8K0B2

Proteomic databases

PaxDbiQ8K0B2
PeptideAtlasiQ8K0B2
PRIDEiQ8K0B2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725 [Q8K0B2-3]
ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725 [Q8K0B2-1]
GeneIDi68421
KEGGimmu:68421
UCSCiuc007amt.1 mouse [Q8K0B2-1]

Organism-specific databases

CTDi55788
MGIiMGI:1915671 Lmbrd1

Phylogenomic databases

eggNOGiENOG410IFHV Eukaryota
ENOG410XPKG LUCA
GeneTreeiENSGT00390000002581
HOGENOMiHOG000068018
HOVERGENiHBG059142
InParanoidiQ8K0B2
KOiK14617
OMAiLNVPNNK
OrthoDBiEOG091G0BP6
PhylomeDBiQ8K0B2
TreeFamiTF329170

Enzyme and pathway databases

ReactomeiR-MMU-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism

Miscellaneous databases

PROiPR:Q8K0B2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073725 Expressed in 271 organ(s), highest expression level in saccule of membranous labyrinth
CleanExiMM_LMBRD1
ExpressionAtlasiQ8K0B2 baseline and differential
GenevisibleiQ8K0B2 MM

Family and domain databases

InterProiView protein in InterPro
IPR006876 LMBR1-like_membr_prot
PfamiView protein in Pfam
PF04791 LMBR1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLMBD1_MOUSE
AccessioniPrimary (citable) accession number: Q8K0B2
Secondary accession number(s): Q3U696
, Q8CCL7, Q8R3D6, Q8VH50, Q9CW67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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