UniProtKB - Q8K0B2 (LMBD1_MOUSE)
Lysosomal cobalamin transport escort protein LMBD1
Lmbrd1
Functioni
GO - Molecular functioni
- cobalamin binding Source: UniProtKB-KW
- insulin receptor binding Source: MGI
GO - Biological processi
- insulin receptor internalization Source: MGI
- negative regulation of glucose import Source: MGI
- negative regulation of insulin receptor signaling pathway Source: MGI
- negative regulation of protein kinase B signaling Source: MGI
Keywordsi
Biological process | Transport |
Ligand | Cobalamin, Cobalt |
Enzyme and pathway databases
Reactomei | R-MMU-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Lysosomal cobalamin transport escort protein LMBD1By similarityShort name: LMBD1 Alternative name(s): LMBR1 domain-containing protein 1 Protein N90b |
Gene namesi | Name:Lmbrd1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915671, Lmbrd1 |
Subcellular locationi
Lysosome
- Lysosome membrane By similarity; Multi-pass membrane protein Sequence analysis
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- clathrin-coated endocytic vesicle Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | ExtracellularSequence analysis | 7 | |
Transmembranei | 8 – 28 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 47 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 48 – 68 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 69 – 97 | ExtracellularSequence analysisAdd BLAST | 29 | |
Transmembranei | 98 – 118 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 141 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 142 – 162 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 163 – 185 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 186 – 206 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 207 – 302 | CytoplasmicSequence analysisAdd BLAST | 96 | |
Transmembranei | 303 – 323 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 324 – 361 | ExtracellularSequence analysisAdd BLAST | 38 | |
Transmembranei | 362 – 382 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 383 – 405 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 406 – 426 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 427 – 483 | ExtracellularSequence analysisAdd BLAST | 57 | |
Transmembranei | 484 – 504 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 505 – 537 | CytoplasmicSequence analysisAdd BLAST | 33 |
Keywords - Cellular componenti
Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000260517 | 1 – 537 | Lysosomal cobalamin transport escort protein LMBD1Add BLAST | 537 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 75 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 85 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 166 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 167 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 235 | PhosphothreonineCombined sources | 1 | |
Glycosylationi | 344 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 445 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 454 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 525 | PhosphoserineCombined sources | 1 | |
Modified residuei | 528 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q8K0B2 |
PaxDbi | Q8K0B2 |
PeptideAtlasi | Q8K0B2 |
PRIDEi | Q8K0B2 |
PTM databases
GlyGeni | Q8K0B2, 7 sites |
iPTMneti | Q8K0B2 |
PhosphoSitePlusi | Q8K0B2 |
SwissPalmi | Q8K0B2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000073725, Expressed in saccule of membranous labyrinth and 286 other tissues |
Genevisiblei | Q8K0B2, MM |
Interactioni
Subunit structurei
Interacts with ABCD4; this interaction induces the translocation of ABCD4 from the endoplasmic reticulum to the lysosome.
Interacts with ABCD4 and MMACHC; this interaction ensures the transport of cobalamin from the lysosome to the cytoplasm.
By similarityGO - Molecular functioni
- insulin receptor binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000140783 |
Miscellaneous databases
RNActi | Q8K0B2, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQ2T, Eukaryota |
GeneTreei | ENSGT00390000002581 |
HOGENOMi | CLU_028341_1_0_1 |
InParanoidi | Q8K0B2 |
OMAi | IGIRFLW |
OrthoDBi | 744771at2759 |
PhylomeDBi | Q8K0B2 |
TreeFami | TF329170 |
Family and domain databases
InterProi | View protein in InterPro IPR006876, LMBR1-like_membr_prot |
Pfami | View protein in Pfam PF04791, LMBR1, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAAAELVI GWCIFGLLLL AILAFCWVYV RKYQSQRESE VVSTVTAIFS
60 70 80 90 100
LAVALITSAL LPVDIFLVSY MKNQNGTFKD WADANVTVQI ENTVLYGYYT
110 120 130 140 150
LYSVILFCVF FWIPFVYFYY EEKDEDDASK CTQIKTALKY TLGFVVICAL
160 170 180 190 200
LLLVGAFVPL HLPNNNNSTE WEKVKLLFED LGTGQGLAAL SFSISSLTLI
210 220 230 240 250
GMLAAITYTA YGMSALPLNL IKGTRSTAYE RLENTEDIEE VEQHIQTIRS
260 270 280 290 300
KSKDGRPLPA RDRRALKQCE ERLRTLRKRE RHLEFIENSW WTKFCGALRP
310 320 330 340 350
LKIIWGIFFI LVALLFVISL FLSNLDKALH SAGIDSGFII FGTNLSNPLN
360 370 380 390 400
MLLPLLQTVF PLDYILITII IMYFIFTSMA GIRNIGIWFF WIRLYKIRRG
410 420 430 440 450
RTRPQALLFL CMILLLIVLH TSYMIYSLAP QYVMYGSQNY LIESNITSDA
460 470 480 490 500
HKGNSTLAVP KRCDADAPKD QCTVTRTYIF LHKFWFFSAA YYFGNWAFLV
510 520 530
VFLIGLIVSC CKGKKSVIEG VDEDSDLSDD EPSAYSA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WS60 | A0A087WS60_MOUSE | Lysosomal cobalamin transport escor... | Lmbrd1 | 161 | Annotation score: | ||
A0A087WPS2 | A0A087WPS2_MOUSE | Lysosomal cobalamin transport escor... | Lmbrd1 | 143 | Annotation score: | ||
A0A087WR85 | A0A087WR85_MOUSE | Lysosomal cobalamin transport escor... | Lmbrd1 | 143 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | P → L in BAC27926 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 173 | K → Q in AAL38052 (Ref. 1) Curated | 1 | |
Sequence conflicti | 193 | S → P in AAL38052 (Ref. 1) Curated | 1 | |
Sequence conflicti | 386 | G → D in BAE31002 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 386 | G → D in BAE31829 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 496 | W → R in BAB22556 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021631 | 1 – 70 | Missing in isoform 3. 3 PublicationsAdd BLAST | 70 | |
Alternative sequenceiVSP_021632 | 1 – 18 | Missing in isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_021633 | 19 – 20 | LL → MQ in isoform 2. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY061989 mRNA Translation: AAL38052.1 AK003084 mRNA Translation: BAB22556.1 AK032560 mRNA Translation: BAC27926.1 AK152170 mRNA Translation: BAE31002.1 AK153237 mRNA Translation: BAE31829.1 AK158982 mRNA Translation: BAE34756.1 AK167080 mRNA Translation: BAE39240.1 BC025579 mRNA Translation: AAH25579.1 BC027774 mRNA Translation: AAH27774.1 BC031902 mRNA Translation: AAH31902.1 BC039053 mRNA Translation: AAH39053.1 |
CCDSi | CCDS35528.1 [Q8K0B2-1] CCDS78556.1 [Q8K0B2-3] |
RefSeqi | NP_001297412.1, NM_001310483.1 [Q8K0B2-3] NP_080995.2, NM_026719.2 [Q8K0B2-1] |
Genome annotation databases
Ensembli | ENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725 [Q8K0B2-3] ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725 [Q8K0B2-1] |
GeneIDi | 68421 |
KEGGi | mmu:68421 |
UCSCi | uc007amt.1, mouse [Q8K0B2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY061989 mRNA Translation: AAL38052.1 AK003084 mRNA Translation: BAB22556.1 AK032560 mRNA Translation: BAC27926.1 AK152170 mRNA Translation: BAE31002.1 AK153237 mRNA Translation: BAE31829.1 AK158982 mRNA Translation: BAE34756.1 AK167080 mRNA Translation: BAE39240.1 BC025579 mRNA Translation: AAH25579.1 BC027774 mRNA Translation: AAH27774.1 BC031902 mRNA Translation: AAH31902.1 BC039053 mRNA Translation: AAH39053.1 |
CCDSi | CCDS35528.1 [Q8K0B2-1] CCDS78556.1 [Q8K0B2-3] |
RefSeqi | NP_001297412.1, NM_001310483.1 [Q8K0B2-3] NP_080995.2, NM_026719.2 [Q8K0B2-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000140783 |
PTM databases
GlyGeni | Q8K0B2, 7 sites |
iPTMneti | Q8K0B2 |
PhosphoSitePlusi | Q8K0B2 |
SwissPalmi | Q8K0B2 |
Proteomic databases
jPOSTi | Q8K0B2 |
PaxDbi | Q8K0B2 |
PeptideAtlasi | Q8K0B2 |
PRIDEi | Q8K0B2 |
Protocols and materials databases
Antibodypediai | 17627, 82 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000095062; ENSMUSP00000092672; ENSMUSG00000073725 [Q8K0B2-3] ENSMUST00000191471; ENSMUSP00000140783; ENSMUSG00000073725 [Q8K0B2-1] |
GeneIDi | 68421 |
KEGGi | mmu:68421 |
UCSCi | uc007amt.1, mouse [Q8K0B2-1] |
Organism-specific databases
CTDi | 55788 |
MGIi | MGI:1915671, Lmbrd1 |
Phylogenomic databases
eggNOGi | ENOG502QQ2T, Eukaryota |
GeneTreei | ENSGT00390000002581 |
HOGENOMi | CLU_028341_1_0_1 |
InParanoidi | Q8K0B2 |
OMAi | IGIRFLW |
OrthoDBi | 744771at2759 |
PhylomeDBi | Q8K0B2 |
TreeFami | TF329170 |
Enzyme and pathway databases
Reactomei | R-MMU-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism |
Miscellaneous databases
BioGRID-ORCSi | 68421, 2 hits in 19 CRISPR screens |
ChiTaRSi | Lmbrd1, mouse |
PROi | PR:Q8K0B2 |
RNActi | Q8K0B2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000073725, Expressed in saccule of membranous labyrinth and 286 other tissues |
Genevisiblei | Q8K0B2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006876, LMBR1-like_membr_prot |
Pfami | View protein in Pfam PF04791, LMBR1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LMBD1_MOUSE | |
Accessioni | Q8K0B2Primary (citable) accession number: Q8K0B2 Secondary accession number(s): Q3U696 Q9CW67 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families