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Protein

Thyrotropin-releasing hormone-degrading ectoenzyme

Gene

Trhde

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific inactivation of TRH after its release.By similarity

Catalytic activityi

Release of the N-terminal pyroglutamyl group from pGlu-|-His-Xaa tripeptides and pGlu-|-His-Xaa-Gly tetrapeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi441Zinc; catalyticPROSITE-ProRule annotation1
Active sitei442Proton acceptorPROSITE-ProRule annotation1
Metal bindingi445Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi464Zinc; catalyticPROSITE-ProRule annotation1
Sitei528Transition state stabilizerBy similarity1

GO - Molecular functioni

  • metalloaminopeptidase activity Source: GO_Central
  • peptide binding Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.008

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotropin-releasing hormone-degrading ectoenzyme (EC:3.4.19.6)
Short name:
TRH-DE
Short name:
TRH-degrading ectoenzyme
Alternative name(s):
Pyroglutamyl-peptidase II
Short name:
PAP-II
TRH-specific aminopeptidase
Thyroliberinase
Gene namesi
Name:Trhde
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2384311 Trhde

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 40CytoplasmicSequence analysisAdd BLAST40
Transmembranei41 – 61Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini62 – 1025ExtracellularSequence analysisAdd BLAST964

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3600275

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000951191 – 1025Thyrotropin-releasing hormone-degrading ectoenzymeAdd BLAST1025

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30Phosphothreonine; by PKCSequence analysis1
Disulfide bondi68InterchainBy similarity
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi223N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi339N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi606N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi635N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi650N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi664N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi685N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi801N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi907N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8K093
PRIDEiQ8K093

PTM databases

PhosphoSitePlusiQ8K093

Expressioni

Gene expression databases

BgeeiENSMUSG00000050663 Expressed in 68 organ(s), highest expression level in pigmented layer of retina
CleanExiMM_TRHDE
GenevisibleiQ8K093 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057449

Structurei

3D structure databases

ProteinModelPortaliQ8K093
SMRiQ8K093
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni405 – 409Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046 Eukaryota
COG0308 LUCA
GeneTreeiENSGT00760000119082
HOGENOMiHOG000106482
HOVERGENiHBG095698
InParanoidiQ8K093
KOiK01306
OMAiYNVFNEY
OrthoDBiEOG091G01GH
PhylomeDBiQ8K093
TreeFamiTF300395

Family and domain databases

CDDicd09601 M1_APN_2, 1 hit
InterProiView protein in InterPro
IPR024571 ERAP1-like_C_dom
IPR034016 M1_APN-typ
IPR001930 Peptidase_M1
IPR014782 Peptidase_M1_N
IPR015570 TRH-DE
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF40 PTHR11533:SF40, 1 hit
PfamiView protein in Pfam
PF11838 ERAP1_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K093-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGEDDAALRA SGRGLSDPWA DSVGVRPRTT ERHIAVHKRL VLAFAVSIVA
60 70 80 90 100
LLAVTMLAVL LSLRFDECGA SAAMPGTDGG LGGFPERDSN SSFPGSARRN
110 120 130 140 150
HHAGGESSQR ESGEVGTPGT PSAQPPSEEE REQWQPWTQL RLSGHLKPLH
160 170 180 190 200
YNLMLTAFME NFTFSGEVNV EIACRNATRY VVLHASRVAV EKVQVAEDRA
210 220 230 240 250
FGAVPVAGFF LYPQTQVLVV VLNRTLDAQR HYNLKIIYNA LIENELLGFF
260 270 280 290 300
RSSYVIHGER RFLGVTQFSP THARKAFPCF DEPIYKATFK ISIKHQATYL
310 320 330 340 350
SLSNMPVETS VFEEDGWVTD HFSQTPLMST YYLAWAICNF TYRETTTKSG
360 370 380 390 400
VVVRLYARPD AIRRGSGDYA LHITKRLIEF YEDYFKVPYS LPKLDLLAVP
410 420 430 440 450
KHPYAAMENW GLSIFVEQRI LLDPSVSSIS YLLDVTMVIV HEICHQWFGD
460 470 480 490 500
LVTPVWWEDV WLKEGFAHYF EFVGTDYLYP AWNMEKQRFL TDVLHEVMLL
510 520 530 540 550
DGLASSHPVS QEVLRATDID RVFDWIAYKK GAALIRMLAN FMGHSVFQRG
560 570 580 590 600
LQDYLTIHKY GNAARNDLWN TLSEALRRNG KYVNIQEVMD QWTLQMGYPV
610 620 630 640 650
ITILGNTTAE NRILITQQHF IYDIGAKTKA LQLQNSSYLW QIPLTIVVGN
660 670 680 690 700
RSHVSSEAII WVSNKSEHHR IAYLDRGSWI LGNINQTGYF RVNYDLRNWR
710 720 730 740 750
LLIDQLIRNH EVLSVSNRAA LIDDAFSLAR AGYLPQNIPL EIIRYLSEEK
760 770 780 790 800
DFLPWHAASR ALYPLDKLLD RMENYNIFNE YILKQVATTY IKLGWPRNNF
810 820 830 840 850
NGSLVQASYQ HEELRREVIM LACSFGNKHC HQQASTLISD WISSNRNRIP
860 870 880 890 900
LNVRDIVYCT GVSLLDEDVW EFIWMKFHST TAVSEKKILL EALTCSDDRN
910 920 930 940 950
LLSRLLNLSL NSEVVLDQDA IDVIIHVARN PHGRDLAWKF FRDKWKILNT
960 970 980 990 1000
RYGEALFMNS KLISGVTEFL NTEGELKELK NFMKSYDGVA SASFSRAVET
1010 1020
VEANVRWKRF YQDELFQWLG KAMRH
Length:1,025
Mass (Da):117,458
Last modified:October 1, 2002 - v1
Checksum:i0990B2A78A13B8AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032288 mRNA Translation: AAH32288.1
CCDSiCCDS24175.1
RefSeqiNP_666353.1, NM_146241.2
UniGeneiMm.445550

Genome annotation databases

EnsembliENSMUST00000061632; ENSMUSP00000057449; ENSMUSG00000050663
GeneIDi237553
KEGGimmu:237553
UCSCiuc007hat.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032288 mRNA Translation: AAH32288.1
CCDSiCCDS24175.1
RefSeqiNP_666353.1, NM_146241.2
UniGeneiMm.445550

3D structure databases

ProteinModelPortaliQ8K093
SMRiQ8K093
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057449

Chemistry databases

ChEMBLiCHEMBL3600275

Protein family/group databases

MEROPSiM01.008

PTM databases

PhosphoSitePlusiQ8K093

Proteomic databases

PaxDbiQ8K093
PRIDEiQ8K093

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061632; ENSMUSP00000057449; ENSMUSG00000050663
GeneIDi237553
KEGGimmu:237553
UCSCiuc007hat.2 mouse

Organism-specific databases

CTDi29953
MGIiMGI:2384311 Trhde

Phylogenomic databases

eggNOGiKOG1046 Eukaryota
COG0308 LUCA
GeneTreeiENSGT00760000119082
HOGENOMiHOG000106482
HOVERGENiHBG095698
InParanoidiQ8K093
KOiK01306
OMAiYNVFNEY
OrthoDBiEOG091G01GH
PhylomeDBiQ8K093
TreeFamiTF300395

Miscellaneous databases

ChiTaRSiTrhde mouse
PROiPR:Q8K093
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050663 Expressed in 68 organ(s), highest expression level in pigmented layer of retina
CleanExiMM_TRHDE
GenevisibleiQ8K093 MM

Family and domain databases

CDDicd09601 M1_APN_2, 1 hit
InterProiView protein in InterPro
IPR024571 ERAP1-like_C_dom
IPR034016 M1_APN-typ
IPR001930 Peptidase_M1
IPR014782 Peptidase_M1_N
IPR015570 TRH-DE
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF40 PTHR11533:SF40, 1 hit
PfamiView protein in Pfam
PF11838 ERAP1_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRHDE_MOUSE
AccessioniPrimary (citable) accession number: Q8K093
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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