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UniProtKB - Q8K045 (PKN3_MOUSE)
Protein
Serine/threonine-protein kinase N3
Gene
Pkn3
Organism
Mus musculus (Mouse)
Status
Functioni
Contributes to invasiveness in malignant prostate cancer.
1 PublicationCatalytic activityi
Activity regulationi
Two specific sites, Thr-707 (activation loop of the kinase domain) and Thr-849 (turn motif), need to be phosphorylated for its full activation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 577 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 673 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 554 – 562 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein kinase C activity Source: UniProtKB-EC
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
- small GTPase binding Source: InterPro
GO - Biological processi
- epithelial cell migration Source: MGI
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-5625740, RHO GTPases activate PKNs R-MMU-8980692, RHOA GTPase cycle R-MMU-9013026, RHOB GTPase cycle R-MMU-9013106, RHOC GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase N3 (EC:2.7.11.13)Alternative name(s): Protein kinase PKN-beta Protein-kinase C-related kinase 3 |
Gene namesi | Name:Pkn3 Synonyms:Pknbeta |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2388285, Pkn3 |
VEuPathDBi | HostDB:ENSMUSG00000026785 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- perinuclear region By similarity
Note: Nuclear and perinuclear Golgi region.By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055726 | 1 – 878 | Serine/threonine-protein kinase N3Add BLAST | 878 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 164 | PhosphoserineBy similarity | 1 | |
Modified residuei | 707 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 711 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 849 | PhosphothreonineBy similarityCurated | 1 |
Post-translational modificationi
Autophosphorylated.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q8K045 |
PaxDbi | Q8K045 |
PRIDEi | Q8K045 |
ProteomicsDBi | 288230 |
PTM databases
iPTMneti | Q8K045 |
PhosphoSitePlusi | Q8K045 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026785, Expressed in cardiac ventricle and 183 other tissues |
ExpressionAtlasi | Q8K045, baseline and differential |
Genevisiblei | Q8K045, MM |
Interactioni
GO - Molecular functioni
- small GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 234455, 4 interactors |
STRINGi | 10090.ENSMUSP00000041025 |
Miscellaneous databases
RNActi | Q8K045, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 77 | REM-1 1PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 86 – 165 | REM-1 2PROSITE-ProRule annotationAdd BLAST | 80 | |
Domaini | 169 – 238 | REM-1 3PROSITE-ProRule annotationAdd BLAST | 70 | |
Domaini | 548 – 807 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 260 | |
Domaini | 808 – 878 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 461 – 525 | DisorderedSequence analysisAdd BLAST | 65 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 476 – 492 | Polar residuesSequence analysisAdd BLAST | 17 |
Domaini
The C1 domain does not bind the diacylglycerol (DAG).
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0694, Eukaryota |
GeneTreei | ENSGT00940000161818 |
HOGENOMi | CLU_000288_132_1_1 |
InParanoidi | Q8K045 |
OMAi | NMNVAAW |
OrthoDBi | 520651at2759 |
PhylomeDBi | Q8K045 |
TreeFami | TF102005 |
Family and domain databases
CDDi | cd11622, HR1_PKN_1, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR035892, C2_domain_sf IPR011072, HR1_rho-bd IPR036274, HR1_rpt_sf IPR011009, Kinase-like_dom_sf IPR017892, Pkinase_C IPR037313, PKN_HR1_1 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF02185, HR1, 3 hits PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
SMARTi | View protein in SMART SM00742, Hr1, 3 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF46585, SSF46585, 3 hits SSF49562, SSF49562, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS51860, REM_1, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8K045-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEHRKPGTGQ RAPKDEKEMV RRAIQKELKI KEGMENMRRV ATDRRHLGHV
60 70 80 90 100
QQLLRASNRR LEQLHGELRE LHAQVLLPAS AEPVTSEPQP RAEQSRARLS
110 120 130 140 150
EALHRQLQVE LKVKQGAENM IHTCASGTPK ERKLLAAAQQ MLKDSQLKVA
160 170 180 190 200
LLRMKISSLE SSGSPEPGPD LLAEELQHRL RVEAAVAAGA KNVVKLLGGQ
210 220 230 240 250
RMQDRKALAE AQAQLQESSQ KLDLLRLALE LLLERLPPTH SLRSRVTQEL
260 270 280 290 300
WMAMLGNPQP LGTLVKPIAL TGTLQVRLLG CKDLLVAVPG RSPMAVLAGS
310 320 330 340 350
PSESWLRTRS RQQRGGGELA SEVLAVLKVD NRVVGQTGWG LVAEKSWDQS
360 370 380 390 400
FIISLDRARE LEIGVHWRDW RQLCGVAFLK LEDFLDNACH QLSLSLVPQG
410 420 430 440 450
RLFAQVTFCE PVIERRPRLQ RQRCIFSKRR GRDFMRASQM NLSMAAWGRL
460 470 480 490 500
VMSLLPPCSS PNTASPPKGR PSTAVCGTPS AASPSNFLPM KTLSKEDTKP
510 520 530 540 550
PPKPPRLYLQ EPAPGTPCTK RPHMDPRPAV VPALAALSTR KPPRLQDFRC
560 570 580 590 600
LAVLGRGHFG KVLLVQYKGT GKYYAIKALK KQEVLGRDEI DSLYCEKRIL
610 620 630 640 650
ETVGRTGHPF LLSLLACLQT SSHACFVTEF LPGGDLMAQI HEDVFPEPQA
660 670 680 690 700
CFYLACVVLG LQFLHEKRII YRDLKLDNLL LDAQGFLKIA DFGLCKEGIG
710 720 730 740 750
FGDRTSTFCG TPEFLAPEVL TQEAYTRAVD WWGLGVLLYE MLVGECPFPG
760 770 780 790 800
DTEEEVFECI VSADVPCPHF LSVQGLELIQ KLLQKSPEKR LGAGERDAEE
810 820 830 840 850
IKVQPFFRTT NWQALLARTV QPPFVPTLCG PADLRYFEGE FTSLPPTLTP
860 870
PVSQSSLTAR QQAAFRDFDF VSEQFLES
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2BE96 | A2BE96_MOUSE | Serine/threonine-protein kinase N3 | Pkn3 | 238 | Annotation score: | ||
A2BE97 | A2BE97_MOUSE | Serine/threonine-protein kinase N3 | Pkn3 | 113 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC034126 mRNA Translation: AAH34126.1 |
CCDSi | CCDS15867.1 |
RefSeqi | NP_722500.1, NM_153805.1 |
Genome annotation databases
Ensembli | ENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785 |
GeneIDi | 263803 |
KEGGi | mmu:263803 |
UCSCi | uc008jbb.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC034126 mRNA Translation: AAH34126.1 |
CCDSi | CCDS15867.1 |
RefSeqi | NP_722500.1, NM_153805.1 |
3D structure databases
SMRi | Q8K045 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234455, 4 interactors |
STRINGi | 10090.ENSMUSP00000041025 |
PTM databases
iPTMneti | Q8K045 |
PhosphoSitePlusi | Q8K045 |
Proteomic databases
MaxQBi | Q8K045 |
PaxDbi | Q8K045 |
PRIDEi | Q8K045 |
ProteomicsDBi | 288230 |
Protocols and materials databases
Antibodypediai | 31249, 186 antibodies from 28 providers |
DNASUi | 263803 |
Genome annotation databases
Ensembli | ENSMUST00000045246; ENSMUSP00000041025; ENSMUSG00000026785 |
GeneIDi | 263803 |
KEGGi | mmu:263803 |
UCSCi | uc008jbb.1, mouse |
Organism-specific databases
CTDi | 29941 |
MGIi | MGI:2388285, Pkn3 |
VEuPathDBi | HostDB:ENSMUSG00000026785 |
Phylogenomic databases
eggNOGi | KOG0694, Eukaryota |
GeneTreei | ENSGT00940000161818 |
HOGENOMi | CLU_000288_132_1_1 |
InParanoidi | Q8K045 |
OMAi | NMNVAAW |
OrthoDBi | 520651at2759 |
PhylomeDBi | Q8K045 |
TreeFami | TF102005 |
Enzyme and pathway databases
Reactomei | R-MMU-5625740, RHO GTPases activate PKNs R-MMU-8980692, RHOA GTPase cycle R-MMU-9013026, RHOB GTPase cycle R-MMU-9013106, RHOC GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 263803, 0 hits in 67 CRISPR screens |
ChiTaRSi | Pkn3, mouse |
PROi | PR:Q8K045 |
RNActi | Q8K045, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026785, Expressed in cardiac ventricle and 183 other tissues |
ExpressionAtlasi | Q8K045, baseline and differential |
Genevisiblei | Q8K045, MM |
Family and domain databases
CDDi | cd11622, HR1_PKN_1, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR035892, C2_domain_sf IPR011072, HR1_rho-bd IPR036274, HR1_rpt_sf IPR011009, Kinase-like_dom_sf IPR017892, Pkinase_C IPR037313, PKN_HR1_1 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF02185, HR1, 3 hits PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
SMARTi | View protein in SMART SM00742, Hr1, 3 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF46585, SSF46585, 3 hits SSF49562, SSF49562, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS51860, REM_1, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PKN3_MOUSE | |
Accessioni | Q8K045Primary (citable) accession number: Q8K045 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families