UniProtKB - Q8JZR0 (ACSL5_MOUSE)
Protein
Long-chain-fatty-acid--CoA ligase 5
Gene
Acsl5
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (By similarity). ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL (By similarity). May have a role in the survival of glioma cells (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity).By similarity
Catalytic activityi
- EC:6.2.1.3By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphateBy similarityEC:6.2.1.15By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 14,15-epoxy-(5Z,8Z,11Z)-eicosatrienoate + ATP + CoA = 14,15-epoxy-(5Z,8Z,11Z)-eicosatrienoyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 11,12-epoxy-(5Z,8Z,14Z)-eicosatrienoate + ATP + CoA = 11,12-epoxy-(5Z,8Z,14Z)-eicosatrienoyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
GO - Molecular functioni
- acyl-CoA ligase activity Source: UniProtKB-EC
- arachidonate-CoA ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- decanoate-CoA ligase activity Source: UniProtKB-EC
- long-chain fatty acid-CoA ligase activity Source: UniProtKB
GO - Biological processi
- fatty acid transport Source: MGI
- lipid biosynthetic process Source: GO_Central
- long-chain fatty acid metabolic process Source: UniProtKB
- long-chain fatty-acyl-CoA biosynthetic process Source: MGI
- phospholipid biosynthetic process Source: MGI
- positive regulation of fatty acid beta-oxidation Source: MGI
- positive regulation of long-chain fatty acid import across plasma membrane Source: MGI
- positive regulation of triglyceride biosynthetic process Source: MGI
- regulation of extrinsic apoptotic signaling pathway Source: MGI
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.2.1.3, 3474 |
Reactomei | R-MMU-75876, Synthesis of very long-chain fatty acyl-CoAs |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919129, Acsl5 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
- Endoplasmic reticulum membrane By similarity; Single-pass type III membrane protein By similarity
Mitochondrion
- Mitochondrion By similarity
- Mitochondrion outer membrane By similarity; Single-pass type III membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrial outer membrane Source: UniProtKB-SubCell
- mitochondrion Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 33 – 683 | CytoplasmicSequence analysisAdd BLAST | 651 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193113 | 1 – 683 | Long-chain-fatty-acid--CoA ligase 5Add BLAST | 683 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 361 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q8JZR0 |
jPOSTi | Q8JZR0 |
MaxQBi | Q8JZR0 |
PaxDbi | Q8JZR0 |
PRIDEi | Q8JZR0 |
PTM databases
iPTMneti | Q8JZR0 |
PhosphoSitePlusi | Q8JZR0 |
SwissPalmi | Q8JZR0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024981, Expressed in small intestine and 330 other tissues |
ExpressionAtlasi | Q8JZR0, baseline and differential |
Genevisiblei | Q8JZR0, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 241124, 4 interactors |
IntActi | Q8JZR0, 2 interactors |
MINTi | Q8JZR0 |
STRINGi | 10090.ENSMUSP00000046585 |
Miscellaneous databases
RNActi | Q8JZR0, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1256, Eukaryota |
GeneTreei | ENSGT00940000156651 |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | Q8JZR0 |
OMAi | LAVPYIF |
OrthoDBi | 630541at2759 |
PhylomeDBi | Q8JZR0 |
TreeFami | TF313877 |
Family and domain databases
Gene3Di | 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q8JZR0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLFIFNFLFS PLPTPALICL LTFGTAIFLW LINRPQPVLP LIDLDNQSVG
60 70 80 90 100
IEGGARRGAF QKNNDLILYY FSDAKTLYEN FQRGLAVSDN GPCLGYRKPN
110 120 130 140 150
QPYKWISYKQ VSDRAEYLGS CLLHKGYKSS QDQFVGIFAQ NRPEWVISEL
160 170 180 190 200
ACYTYSMVAV PLYDTLGTEA IIFVINRADI PVVICDTPQK ATMLVENVEK
210 220 230 240 250
GLTPGLKTII LMDPFDDDLM KRGEKCGVEM LSLHDAENIG KENFKKPVPP
260 270 280 290 300
KPEDLSVICF TSGTTGDPKG AMLTHENVVS NMAAFLKFLE PIFQPTSDDV
310 320 330 340 350
TISYLPLAHM FERLVQGILF SCGGKIGFFQ GDIRLLPDDM KALKPTVFPT
360 370 380 390 400
VPRLLNRVYD KVQNEAKTPL KKFLLNLAII SKFNEVKNGI IRRDSLWDKL
410 420 430 440 450
VFSKIQGSLG GKVRLMITGA APISTPVLTF FRAAMGCWVF EAYGQTECTG
460 470 480 490 500
GCSITSPGDW TAGHVGTPVA CNFVKLEDVA DMNYFSVNNE GEICIKGNNV
510 520 530 540 550
FKGYLKDPEK TQEVLDKDGW LHTGDIGRWL PNGTLKIVDR KKNIFKLAQG
560 570 580 590 600
EYIAPEKIEN VYSRSRPVLQ VFVHGESLRS FLIGVVVPDP DSLPSFAAKI
610 620 630 640 650
GVKGSFEELC KNQCVKEAIL EDLQKIGKEG GLKSFEQVKS IFVHPEPFTI
660 670 680
ENGLLTPTLK AKRVELAKFF QTQIKSLYES IEE
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A286YE29 | A0A286YE29_MOUSE | Long-chain-fatty-acid--CoA ligase 5 | Acsl5 | 95 | Annotation score: | ||
A0A286YD68 | A0A286YD68_MOUSE | Long-chain-fatty-acid--CoA ligase 5 | Acsl5 | 117 | Annotation score: | ||
A0A286YCF3 | A0A286YCF3_MOUSE | Long-chain-fatty-acid--CoA ligase 5 | Acsl5 | 62 | Annotation score: | ||
A0A286YCG4 | A0A286YCG4_MOUSE | Long-chain-fatty-acid--CoA ligase 5 | Acsl5 | 96 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC031544 mRNA Translation: AAH31544.1 |
CCDSi | CCDS29908.1 |
PIRi | S49487 |
RefSeqi | NP_082252.1, NM_027976.2 XP_006527241.1, XM_006527178.2 |
Genome annotation databases
Ensembli | ENSMUST00000043150; ENSMUSP00000046585; ENSMUSG00000024981 |
GeneIDi | 433256 |
KEGGi | mmu:433256 |
UCSCi | uc008hxp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC031544 mRNA Translation: AAH31544.1 |
CCDSi | CCDS29908.1 |
PIRi | S49487 |
RefSeqi | NP_082252.1, NM_027976.2 XP_006527241.1, XM_006527178.2 |
3D structure databases
SMRi | Q8JZR0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 241124, 4 interactors |
IntActi | Q8JZR0, 2 interactors |
MINTi | Q8JZR0 |
STRINGi | 10090.ENSMUSP00000046585 |
PTM databases
iPTMneti | Q8JZR0 |
PhosphoSitePlusi | Q8JZR0 |
SwissPalmi | Q8JZR0 |
Proteomic databases
EPDi | Q8JZR0 |
jPOSTi | Q8JZR0 |
MaxQBi | Q8JZR0 |
PaxDbi | Q8JZR0 |
PRIDEi | Q8JZR0 |
Protocols and materials databases
Antibodypediai | 31809, 257 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043150; ENSMUSP00000046585; ENSMUSG00000024981 |
GeneIDi | 433256 |
KEGGi | mmu:433256 |
UCSCi | uc008hxp.1, mouse |
Organism-specific databases
CTDi | 51703 |
MGIi | MGI:1919129, Acsl5 |
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
GeneTreei | ENSGT00940000156651 |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | Q8JZR0 |
OMAi | LAVPYIF |
OrthoDBi | 630541at2759 |
PhylomeDBi | Q8JZR0 |
TreeFami | TF313877 |
Enzyme and pathway databases
BRENDAi | 6.2.1.3, 3474 |
Reactomei | R-MMU-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
BioGRID-ORCSi | 433256, 0 hits in 18 CRISPR screens |
ChiTaRSi | Acsl5, mouse |
PROi | PR:Q8JZR0 |
RNActi | Q8JZR0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024981, Expressed in small intestine and 330 other tissues |
ExpressionAtlasi | Q8JZR0, baseline and differential |
Genevisiblei | Q8JZR0, MM |
Family and domain databases
Gene3Di | 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACSL5_MOUSE | |
Accessioni | Q8JZR0Primary (citable) accession number: Q8JZR0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families