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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

Eif3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 9UniRule annotation
eIF-3-etaUniRule annotation
eIF3 p116
Gene namesi
Name:Eif3b
Synonyms:Eif3s9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:106478 Eif3b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryonic death.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002234791 – 803Eukaryotic translation initiation factor 3 subunit BAdd BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineUniRule annotationBy similarity1
Modified residuei31PhosphothreonineCombined sources1
Modified residuei37PhosphoserineCombined sources1
Modified residuei40PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei72PhosphothreonineCombined sources1
Modified residuei74PhosphothreonineCombined sources1
Modified residuei75PhosphoserineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Modified residuei88PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Modified residuei111PhosphoserineCombined sources1
Modified residuei120PhosphoserineCombined sources1
Modified residuei123PhosphoserineCombined sources1
Modified residuei141PhosphoserineUniRule annotationBy similarity1
Modified residuei143PhosphoserineUniRule annotationBy similarity1
Modified residuei153PhosphoserineUniRule annotationBy similarity1
Modified residuei198N6-acetyllysineBy similarity1
Modified residuei228PhosphoserineUniRule annotationBy similarity1
Modified residuei277N6-acetyllysineBy similarity1
Modified residuei353N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8JZQ9
MaxQBiQ8JZQ9
PaxDbiQ8JZQ9
PRIDEiQ8JZQ9

PTM databases

iPTMnetiQ8JZQ9
PhosphoSitePlusiQ8JZQ9
SwissPalmiQ8JZQ9

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000056076 Expressed in 294 organ(s), highest expression level in testis
ExpressionAtlasiQ8JZQ9 baseline and differential
GenevisibleiQ8JZQ9 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 and HNRPD. Interacts with METTL3.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Eif4g1Q6NZJ62EBI-4286513,EBI-8175606

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205699, 5 interactors
IntActiQ8JZQ9, 14 interactors
MINTiQ8JZQ9
STRINGi10090.ENSMUSP00000098076

Structurei

3D structure databases

ProteinModelPortaliQ8JZQ9
SMRiQ8JZQ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini174 – 257RRMUniRule annotationAdd BLAST84
Repeati321 – 359WD 1Add BLAST39
Repeati361 – 406WD 2Add BLAST46
Repeati410 – 448WD 3Add BLAST39
Repeati549 – 590WD 4Add BLAST42
Repeati638 – 683WD 5Add BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 402Sufficient for interaction with EIF3EUniRule annotationAdd BLAST290
Regioni159 – 263Sufficient for interaction with EIF3JUniRule annotationAdd BLAST105

Domaini

The RRM domain mediates interaction with EIF3J.UniRule annotation

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2314 Eukaryota
COG5354 LUCA
GeneTreeiENSGT00550000074913
HOGENOMiHOG000265546
HOVERGENiHBG006127
InParanoidiQ8JZQ9
KOiK03253
OMAiNFAWEPK
OrthoDBiEOG091G02V5
PhylomeDBiQ8JZQ9
TreeFamiTF101521

Family and domain databases

CDDicd12278 RRM_eIF3B, 1 hit
Gene3Di2.130.10.10, 2 hits
3.30.70.330, 1 hit
HAMAPiMF_03001 eIF3b, 1 hit
InterProiView protein in InterPro
IPR011400 EIF3B
IPR034363 eIF3B_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR013979 TIF_beta_prop-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
PANTHERiPTHR14068 PTHR14068, 1 hit
PfamiView protein in Pfam
PF08662 eIF2A, 1 hit
PF00076 RRM_1, 1 hit
PIRSFiPIRSF036424 eIF3b, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8JZQ9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQDAENVAVP EAAEERAEPA RQQPASESPP TDEAAGSGGS EVGQTEDAEE
60 70 80 90 100
DAEAGPEPEV RAKPAAQSEE ETATSPAASP TPQSAERSPS QEPSAPGKAE
110 120 130 140 150
AVGEQARGHP SAGAEEEGGS DGSAAEAEPR ALENGEADEP SFSDPEDFVD
160 170 180 190 200
DVSEEELLGD VLKDRPQEAD GIDSVIVVDN VPQVGPDRLE KLKNVIHKIF
210 220 230 240 250
SKFGKIINDY YPEEDGKTKG YIFLEYASPA HAVDAVKNAD GYKLDKQHTF
260 270 280 290 300
RVNLFTDFDK YMTISDEWDI PEKQPFKDLG NLRYWLEEAE CRDQYSVIFE
310 320 330 340 350
SGDRTSIFWN DVKDPVSIEE RARWTETYVR WSPKGTYLAT FHQRGIALWG
360 370 380 390 400
GDKFKQIQRF SHQGVQLIDF SPCERYLVTF SPLMDTQDDP QAIIIWDILT
410 420 430 440 450
GHKKRGFHCE SSAHWPIFKW SHDGKFFARM TLDTLSIYET PSMGLLDKKS
460 470 480 490 500
LKISGIKDFS WSPGGNIIAF WVPEDKDIPA RVTLMQLPTR QEIRVRNLFN
510 520 530 540 550
VVDCKLHWQK NGDYLCVKVD RTPKGTQGVV TNFEIFRMRE KQVPVDVVEM
560 570 580 590 600
KETIIAFAWE PNGSKFAVLH GEAPRISVSF YHVKSNGKIE LIKMFDKQQA
610 620 630 640 650
NTIFWSPQGQ FVVLAGLRSM NGALAFVDTS DCTVMNIAEH YMASDVEWDP
660 670 680 690 700
TGRYVVTSVS WWSHKVDNAY WLWTFQGRLL QKNNKDRFCQ LLWRPRPPTL
710 720 730 740 750
LSQDQIKQIK KDLKKYSKIF EQKDRLSQSK ASKELVERRR TMMEDFRQYR
760 770 780 790 800
KMAQELYMKQ KNERLELRGG VDTDELDSNV DDWEEETIEF FVTEEVIPLG

SQE
Length:803
Mass (Da):91,370
Last modified:October 1, 2002 - v1
Checksum:iD770AC70BFE9832F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JG48A0A0G2JG48_MOUSE
Eukaryotic translation initiation f...
Eif3b
175Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033722 mRNA Translation: BAC28445.1
AK167618 mRNA Translation: BAE39671.1
AK170938 mRNA Translation: BAE42128.1
BC007175 mRNA Translation: AAH07175.1
BC031704 mRNA Translation: AAH31704.1
CCDSiCCDS19819.1
PIRiJC7862
RefSeqiNP_598677.1, NM_133916.2
UniGeneiMm.21671

Genome annotation databases

EnsembliENSMUST00000100507; ENSMUSP00000098076; ENSMUSG00000056076
GeneIDi27979
KEGGimmu:27979
UCSCiuc009ahr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033722 mRNA Translation: BAC28445.1
AK167618 mRNA Translation: BAE39671.1
AK170938 mRNA Translation: BAE42128.1
BC007175 mRNA Translation: AAH07175.1
BC031704 mRNA Translation: AAH31704.1
CCDSiCCDS19819.1
PIRiJC7862
RefSeqiNP_598677.1, NM_133916.2
UniGeneiMm.21671

3D structure databases

ProteinModelPortaliQ8JZQ9
SMRiQ8JZQ9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205699, 5 interactors
IntActiQ8JZQ9, 14 interactors
MINTiQ8JZQ9
STRINGi10090.ENSMUSP00000098076

PTM databases

iPTMnetiQ8JZQ9
PhosphoSitePlusiQ8JZQ9
SwissPalmiQ8JZQ9

Proteomic databases

EPDiQ8JZQ9
MaxQBiQ8JZQ9
PaxDbiQ8JZQ9
PRIDEiQ8JZQ9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100507; ENSMUSP00000098076; ENSMUSG00000056076
GeneIDi27979
KEGGimmu:27979
UCSCiuc009ahr.1 mouse

Organism-specific databases

CTDi8662
MGIiMGI:106478 Eif3b

Phylogenomic databases

eggNOGiKOG2314 Eukaryota
COG5354 LUCA
GeneTreeiENSGT00550000074913
HOGENOMiHOG000265546
HOVERGENiHBG006127
InParanoidiQ8JZQ9
KOiK03253
OMAiNFAWEPK
OrthoDBiEOG091G02V5
PhylomeDBiQ8JZQ9
TreeFamiTF101521

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

ChiTaRSiEif3b mouse
PROiPR:Q8JZQ9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056076 Expressed in 294 organ(s), highest expression level in testis
ExpressionAtlasiQ8JZQ9 baseline and differential
GenevisibleiQ8JZQ9 MM

Family and domain databases

CDDicd12278 RRM_eIF3B, 1 hit
Gene3Di2.130.10.10, 2 hits
3.30.70.330, 1 hit
HAMAPiMF_03001 eIF3b, 1 hit
InterProiView protein in InterPro
IPR011400 EIF3B
IPR034363 eIF3B_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR013979 TIF_beta_prop-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
PANTHERiPTHR14068 PTHR14068, 1 hit
PfamiView protein in Pfam
PF08662 eIF2A, 1 hit
PF00076 RRM_1, 1 hit
PIRSFiPIRSF036424 eIF3b, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3B_MOUSE
AccessioniPrimary (citable) accession number: Q8JZQ9
Secondary accession number(s): Q922K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2002
Last modified: October 10, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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